PRIMING OF SEEDS WITH DIFFERENT SEAWEED EXTRACTS TO MITIGATE SALINITY STRESS IN PADDY var.

ANNA 4. Ambika, S*, K. Sujatha and K. Balakrishnan

Department of Seed Science and Technology, Agricultural College and Research Institute, Madurai-625 104

*Corresponding author: E-mail: ambikasingaram@gmail.com

Ambika, S*, K. Sujatha and K. Balakrishnan

Department of Seed Science and Technology, Agricultural College and Research Institute, Madurai-625 104

*Corresponding author: E-mail: ambikasingaram@gmail.com

Rice is one of the most important food crops for over half of the world’s population. In Asia alone, 21.5 million ha are affected, 12 million ha of

which are saline and 9.5 million ha are alkaline/sodic (Lautte et al., 2006). Effects of salt stress include decreased germination and seedling growth (Ahmad et

al., 2010) and dry matter production. Seaweeds - rich source - growth promoting substances, metabolic enhancers, macro and micro elements, amino acids,

vitamins -results -early seed germination and establishment, improved crop performance and yield, elevated resistance to biotic and abiotic stress (Bluden,

1994).

Collection and preparation of extractsFresh - Mandapam coast

 Washed -remove all epiphytes and sand particles with tap water

Shade dried for five days and oven drying at 40oC for 24 h and powdered

150 ml of alcohol was added to 20 g powder and kept for overnight with intermittent stirring

SLF extracted through rotary evaporator at 40oC and 45 RPM.

The 100% seaweed extract was collected stored in air tight container

Different oncentrations of (10, 20, and 30%) of seaweed extracts were prepared from 100% concentrated solution

Fig 3 . Effect os seaweed extracts on vigour index of rice seeds under saline conditions

Seaweeds Concentrations Soaking duration Salinity level (%)

Sargassum myricocystum 10 12 h

0.25 %

Gracillaria edulis 20 0.50 %

Caulerpa racemosa 30 0.75%

1.0%Untreated control

Seeds primed with S. myricocystum (10 %) enhanced the germination in all the

salinity level and per cent increase over control was 4.2, 6.5, 9.3 and 6.4 under 0.25, 0.5,

0.75 and 1.0 % salinity, respectively (fFig 1 and 2).

S. myricocystum (10 %) primed seeds enhanced the seedling length in all the salinity

level and per cent increase over untreated control was 5.6, 5.7, 11.4 and 9.6 under 0.25,

0.5, 0.75 and 1.0 % salinity, respectively .

Percent of vigour index increase over untreated control seed was 9.6, 11.9, 19.6 and

15.5 under 0.25, 0.5, 0.75 and 1.0 % salinity, respectively for seeds primed with S.

myricocystum (10 %) . Dry matter production in S. myricocystum (10 %) was 0.13,

0.09, 0.08 and 0.05 (g seedlings-10) at 0.25, 0.5, 0.75 and 1.0%, respectively where as

control recorded 0.10, 0.08, 0.07 and 0.043 at 0.25, 0.5, 0.75 and 1.0%, respectively.

The seeds primed with S. myricocystum (20 and 30%), G. edulis (10, 20 and 30 %)

and C. racemosa (10, 20 and 30 %) recorded nil germination (%), seedling length (cm),

vigour index and dry matter production in all the salinity level.

Bean seeds primed with Sargassum vulgare (0.2 and 0.5%) recorded higher

germination rate,seedling length and Seedling fresh and dry weight under salt stress

conditions (Latique Salma et al., 2014).

Paddy seeds primed with S. myricocystum (10 %) can with stand the

low and high salinity levels and enhances faster and uniform

germination, seelding length , vigour index and dry weight compared

to control.

 

Fig 1. Effect os seaweed extracts on speed of germination (%) of rice seeds under saline condition (1.0 %)

Control

S. myricocystum (10%)

Salt stress- 1.0 %

Fig 2. Effect os seaweed extracts on speed of germination (%) of rice seeds under saline condition (1.0 %)

Control

S. myricocystum (10%)

S. myricocystum (10%)

Latique Salma, Elouaer Mohamed Aymen, Souguir Maher, Aloui Hassen, Hannachi Cherif, Chernane Halima, Mansori Mounir, Elkaoua Mimoun. 2014. Effect of seaweed extract of S. vulgare on germination behavior of two bean cultivars under salt stress. Journal of Agriculture and Veterinary Science, 7(2): 116-120.