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Proceeding of 2 nd ICST 2017 Influence of Coconut Shell Biochar and Dose Biocompost (Granules and Liquid Form) Fermented with Trichoderma spp. Against Growth and Wilt Disease on Soybean U’ul Efriyanti Prayoba 1)* , I Made Sudantha 2) , Suwardji 2) 1) Student of Postgraduate Study Program on Dryland Resources Management, University of Mataram Jl. Pendidikan No. 37 Mataram 83125. 2) Lecturer of Postgraduate Study Program on Dryland Resources Management, University of Mataram Jl. Pendidikan No. 37 Mataram 83125. Corresponding Author: [email protected] ABSTRACT The purpose of this study was to determine the effect of cocunut shell biochar and the dosage of biocompost (granules and liquid form) fermented with Trichoderma spp. fungus on the growth of soybean and the incident of wilt disease in soybean. The study used Split Plot Design with two factors. As the main plot is cocunut shell biochar which consists of two levels, namely: without biochar and with biochar. As a subplot is a biocompost (granules and liquid form) dose consisting of seven levels, ie without biocompost, 2.5 g granules form/ plant, 5.0 g granules form/ plant, 7.5 g granules form/ plant, 2.5 ml biocompost liquid form/ plant, 5.0 ml liquid form/ plant, and 7.5 ml liquid form/ plant. The treatment was a combination of biochar factor and biocompost (granules and liquid form) dose, each repeated three times so that there were 42 experimental units. The results showed that the higher doses of biocompost granules and liquid form that accompanied the provision of cocunut shell biochar can increase soybean plant growth and can suppress the incident of wilt disease in soybean. Keywords: Biochar, biocompost, Trichoderma spp., wilt disease, soybean. 1. Introduction In the area of NTB, soybeans are the main crops grown with soybean-soybean-corn or soybean-corn-soybean cropping patterns. The productivity of soybean yield at the farmer level has reached 1.0 ton / ha, and this result is still low because the biological potential of soybean yield in dry land in NTB can reach 3.3 ton / ha, and the average research result has reached 2, 5 tons / ha or 75 percent of its biological potential. This means that soybean crops have the potential to be developed and improved productivity (Sudantha 2009). One of the main limiting factors in the development of soybean crops on dry land is the health of soybean crops due to the onslaught of soil pathogens such as the fungus Fusarium oxysporum f. sp. glycine causes of the disease falling sprouts and wither. This soil pathogens 442

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Page 1: Proceeding of 2nd ICST 2017 - UNRAMeprints.unram.ac.id/4495/1/Proceeding of 2nd ICST 2017... · 2018. 5. 31. · Proceeding of 2nd ICST 2017 Influence of Coconut Shell Biochar and

Proceeding of 2nd ICST 2017 Influence of Coconut Shell Biochar and Dose Biocompost (Granules and

Liquid Form) Fermented with Trichoderma spp. Against Growth and Wilt

Disease on Soybean

U’ul Efriyanti Prayoba1)*, I Made Sudantha2), Suwardji2)

1) Student of Postgraduate Study Program on Dryland Resources Management, University of

Mataram Jl. Pendidikan No. 37 Mataram 83125.

2) Lecturer of Postgraduate Study Program on Dryland Resources Management, University of

Mataram

Jl. Pendidikan No. 37 Mataram 83125.

Corresponding Author: [email protected]

ABSTRACT

The purpose of this study was to determine the effect of cocunut shell biochar and the dosage

of biocompost (granules and liquid form) fermented with Trichoderma spp. fungus on the

growth of soybean and the incident of wilt disease in soybean. The study used Split Plot Design with two factors. As the main plot is cocunut shell biochar which consists of two

levels, namely: without biochar and with biochar. As a subplot is a biocompost (granules and

liquid form) dose consisting of seven levels, ie without biocompost, 2.5 g granules form/

plant, 5.0 g granules form/ plant, 7.5 g granules form/ plant, 2.5 ml biocompost liquid form/ plant, 5.0 ml liquid form/ plant, and 7.5 ml liquid form/ plant. The treatment was a

combination of biochar factor and biocompost (granules and liquid form) dose, each repeated

three times so that there were 42 experimental units. The results showed that the higher doses of biocompost granules and liquid form that accompanied the provision of cocunut shell

biochar can increase soybean plant growth and can suppress the incident of wilt disease in

soybean.

Keywords: Biochar, biocompost, Trichoderma spp., wilt disease, soybean.

1. Introduction

In the area of NTB, soybeans are the main crops grown with soybean-soybean-corn or

soybean-corn-soybean cropping patterns. The productivity of soybean yield at the farmer

level has reached 1.0 ton / ha, and this result is still low because the biological potential of

soybean yield in dry land in NTB can reach 3.3 ton / ha, and the average research result has

reached 2, 5 tons / ha or 75 percent of its biological potential. This means that soybean crops

have the potential to be developed and improved productivity (Sudantha 2009).

One of the main limiting factors in the development of soybean crops on dry land is the

health of soybean crops due to the onslaught of soil pathogens such as the fungus Fusarium

oxysporum f. sp. glycine causes of the disease falling sprouts and wither. This soil pathogens

442

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are difficult to control, one of the reasons for the difficulty in controlling these pathogens is

due to the ability of the pathogen to maintain itself in the soil even though no host is available

in the form of a resting structure resistant to extreme soil conditions of clamidospores with a

survival ability of up to 10 years . This propagule is very important as a primary inoculum for

the occurrence of infection in the next planting season (Sudantha, 2009).

Preliminary results of the use of endophytic fungus Trichoderma polysporum isolates

ENDO-04 and saprophytic fungus T. harzianum SAPRO-07 isolates in-vitro and in-vivo (in

laboratory) and in-situ (in greenhouse) effectively controlled wilt disease caused by the

fungus F. oxysporum f. sp. glycine up to 90%. Similarly, both types of fungus Trichoderma

spp. this as a biocompost can spur vegetative growth and early flower formation (Sudantha,

2010).

Biochar is a black charcoal made from organic biomass heating process in limited

oxygen state, used as an alternative to soil enhancers especially for soils and degraded soils

or low fertility soils (Sudantha and Suwardji, 2014).

The purpose of this study was to determine the effect of cocunut shell biochar and the

dosage of biocompost (granules and liquid form) fermented with Trichoderma spp. fungus on

the growth of soybean and the incident of wilt disease in soybean.

2. Material and Method

Materials Research

The biocompost used is a biocompost of granules and liquid formulations. The granules

and liquid formulations were prepared based on the Sudantha method (2009). The fungi

cultures used were T. koningii isolates ENDO-02 and T. harzianum isolates SAPRO-07

which is a fungus in pure culture grown on PDA media.

Biochar comes from the remnants of coconut shell charcoal from Small Industry of

Charcoal Making in Bengkaung Village, Batulayar Sub District, West Lombok Regency.

Further biochar sprayed with a solution of mixture of endophytic fungus T. koningii (ENDO-

02 isolate) and saprofit fungus T. harzianum (SAPRO-07 isolate) evenly while the compost

material is stirred, until the water content reaches 30-40%. Furthermore, the pile of biochar

closed tightly with tarpaulins and left for 3 weeks with a reversal every once a week.

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Soybean seeds used in the research are Anjasmoro varieties obtained from Central

Main Seed of Agriculture NTB Province Jl. Raya Peninjauan Km 8, Narmada West Lombok

District.

Research methods

The design used is Split Plot Design with two factors. Main plot is biochar which

consists of two levels, namely: without biochar, and with biochar. The plot is a dose of

biocompost (a form of fermented granules and liquid fungus Trichoderma spp.) Consisting of

seven levels, namely: without biocompost, biocompost 2.5 g granules / plant, biocompost 5.0

g granules / plant, biocompost 7.5 g granules / plant, biocompost 2.5 ml liquid / plant,

biocompost 5.0 ml liquid / plant, biocompost 7.5 ml liquid / plant. The treatment was a

combination of biochar factor and biocompost dose (granules and liquid form) each repeated

three times so that there were 42 experimental units. Ways of working

Soil treatment is done using hoe to level the soil and then made a plot with size 2 m × 4

m for each treatment plot. After basic soil fertilization using Phonska fertilizer 100 kg / ha

(50% from recommendation). Provision of basic fertilizer is done by means immersed in the

next planting hole.

Application of biochar and biocompost is done at the time of soybean seeds. The

application of biochar (15 g / plant) and biocompost granules (15 g / plant) or liquid

biocompost (15 ml / plant) was performed simultaneously with the applying of soybean seeds

on the sides of the plant hole (about 5 cm from the planting hole). The soybean seed is placed

into a hole with a depth of 2 cm and the hole is closed again with soil. Planting done with

spacing 20 × 40 cm.

Plant maintenance includes fertilizing, irrigation and weeding. Subsequent fertilization

at 5 weeks after planting plants. Subsequent fertilization using urea fertilizer 165 kg / ha and

KCl 50 kg / ha. Fertilization is done by placing the fertilizer next to the base of the stem of

the plant with a distance of ± 2cm. Irrigation is done in the morning or afternoon and done by

way of experiments dileb through the channel on the trial plot to the wet soil. Weeding is

done by removing weeds that grow around the plant.

The variables observed were plant growth (plant height) and incidence of Fusarium wilt

disease. Plant height is measured from the base of the stem to the highest tip of soybean crop

(cm). Plant height measurements were performed until the plant was 56 days after planting.

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Observation of disease incidence is done by counting the number of wilting plants,

observations made until the plant is 56 days after planting. The incidence of the disease (%)

is calculated using the following formula:

where:

I= Percentage of disease incidence

a= Number of plants showing symptoms of the

disease b = Total number of plants observed

Data analysis

The observed data were analyzed using Analysis of Variance (ANOVA) with 5% real

level using Minitab for Windows Rel. 13. If there is a variation then tested continued by

using the test of Honestly Significant Difference (HSD) at a real level of 5%.

3. Results and Discussion

High Soybean Plants

The results of the analysis showed that there was a real interaction between biochar

application and the biocompost dose fermented with Trichoderma sp fungus. Further test

results to know which interactions were significantly different were presented in Table 1.

In Table 1 it was shown that all treated doses of fermented biocomposts of

Trichoderma sp. in the form of granuless and liquid different from the control both on

treatment without biochar and with biochar to soybean plant height at age 56 HST. The

higher the biocompost dosage form of granuless and liquid soybean crop is also higher

growth. Each dose of biocompost application that is accompanied by biochar gives a better

soybean plant growth when compared with no biochar.

This difference in plant height is thought to be due to the role of biochar fermented with

Trichoderma spp fungus, namely: Saprofit fungus T. harzainum isolates SAPRO-07 and

endophytic fungus T. koningii isolates ENDO-02 used for biochar fermentation can colonize

well in biochar which is then given into the soil. In this study was found population of

Trichoderma spp. in biochar and in the soybean plant 6.0 x 106 propagule / g soil. This

means that biochar is a good host for Trichoderma spp. According to Sudantha (2007) that

these two species of mushrooms have different characters, namely saprophytic fungus T.

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Proceeding of 2nd ICST 2017 harzainum isolates SAPRO-07 colonize in soybean plant rhizosphere and endophytic fungus

T. koningii isolates ENDO-02 enter into soybean plant tissue. Furthermore Sudantha (2010b)

said that endophytic fungi in soybean plant tissue play a more important role in spurring

vegetative growth compared to generative, and vice versa saprofit fungi is more important in

spurring generative growth compared with vegetative.

In the role of endophytic fungus T. koningii isolates ENDO-02 in soybean plant tissue

stimulates ethylene in spurring elongation of the cell to increase the height of the plant,

whereas the Saprofit T. harzainum SAPRO-07 saprophytic fungus in the rhizosphere or root

zone of soybean plants dispenses ethylene to diffuse crop body through silem that contribute

to spur generative growth. Biochar that has been fermented with both species of fungi has a

direct effect on improving the physical, chemical and biological properties of the soil. Thus,

good physical, chemical and biological properties of the soil can increase the growth and

yield of soybean crops. In this study, soil analysis after biochar administration showed that

soil pH increased from 6.0 to 6.2; there was an increase of organic C from 1.93% to 2.09%;

total N increase from 0.16% to 0.44%; an increase of P is available from 0.46 ppm to 43.86

ppm, an increase in K is switched from 1.28 M% to 2.68 M%; and an increase in CEC from

11.25% Me to 17.67 Me%. According to Sukartono and Utumo (2012), the increase of soil

pH at biochar treatment is related to raw material of biochar which is used is coconut shell

which has high alkaline level. Further Priyono (2005) said that soil pH effect on the

availability of nutrients because it is one of the important soil chemical properties that can

determine the quality of soil as a medium for growing plants. Lehman et al. (2006) says that

the increase of C-Organic on biochar treatment caused by the C-organic content of biochar

has an aromatic C structure that is more resistant to decomposition, so that the presence of C-

Organic on the soil increases even longer.

According to Islami (2012) that the increase of C-Organic soil has an implication to the

increase of organic material content in the soil and will also have an effect on soil quality

improvement and nutrient presence in the soil because soil organic matter is one of the keys

that determine fertility and soil productivity. Organic materials are the main source of some

nutrient elements, especially N, P, S and most of K. Priyono (2005) argues that the increase

of CEC value in biochar treatment shows improvement of soil properties after application

because the CEC value of a soil has a close relationship with the level soil fertility.

Furthermore, Islami (2012) stated that the increase of fertilizer efficiency occurs as a result of

the high CEC in the biochar treatment so that it can absorb nutrients in fertilizer and

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subsequently minimize nutrient loss due to leaching. Sukartono (2011) said that the increase

of Total N content in soils after biochar application is closely related to the increase of C-

Organic in soil. Increasing C-Organic in the soil can further increase the content of organic

matter so that it will also affect the increase of nitrogen in the soil because one of the main

sources of nitrogen in the soil is organic matter. Nurida and Rachman (2012) say that this

increase in available P and K is correlated with increasing the pH and CEC in the soil after

the biochar application. CEC value of soil can be an indicator of soil fertility in this case is

able to provide nutrients P and K in the soil for the growth and development of plants.

Tabel 1. The average height of soybean plant at age 56 days after planting on biochar interaction and dose of fermented biocompost of Trichoderma sp.

Biochar HSD 5%

Dose of Biocompost

Without biochar

With biochar

0 g granuless/plants 43,30 a *) 49,07 b

A **) A

2,5 g granuless/plants 45,44 a 52,10 b

B B

5,0 g granuless/plants 48,19 a 54,19 b

C C

7,5 g granuless/plants 52,00 a 56,26 b

D D 2,04

2,5 ml liquids/plants 47,50 a

54,00 b

B B

5,0 ml liquids/plants 50,19 a 56,10 b

C C

7,5 ml liquids/plants 52,40 a 58,10 b

D D

HSD 5% 2,04

*) The numbers followed by the same letter in the same column are not significantly different in the 5% HSD

test. **) The numbers followed by the same letter in the same line are not significantly different in the 5% HSD test.

447

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The incidence of lute disease in soybean plants

The results showed that there was a real interaction between biochar application and

biochar dose fermented with Trichoderma sp fungus. Further test results to know which

interaction was significantly different were presented in Table 2.

In Table 2 it was shown that all treated doses of fermented biocomposts of

Trichoderma sp. in granules and liquid form differed with controls in both biochar and

biochar treatments against wilt disease in soybean plants of 56 HST. The higher the dosage of

biocompost form of granules and liquid the percentage of wilt disease in soybean crop is

lower. Each application of biocompost dose accompanied by biochar showed that the

percentage of wilting disease was lower when compared with no biochar

Tabel 2. The mean percentage of wilt disease in soybean plants was 56 days after planting in biochar interaction and dose of fermented biocompost of Trichoderma sp.

Dose of Biocompost Biochar HSD 5%

Without With

biochar biochar

0 g granuless/plants 15,17 a *) 10,07 b

A **) A

2,5 g granuless/plants 7,24 a 5,44 b

B B

5,0 g granuless/plants 5,15 a 3,19 b

C C

7,5 g granuless/plants 3,20 a 1,00 b

D D 2,10

2,5 ml liquids/plants 7,30 a 5,34 b

B B

5,0 ml liquids/plants 5,20 a 3,15 b

C C

7,5 ml liquids/plants 3,25 a 1,15 b

D D

HSD 5% 2,10

*) The numbers followed by the same letter in the same column are not significantly different in the 5% HSD

test. **) The numbers followed by the same letter in the same line are not significantly different in the 5% HSD test.

448

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Proceeding of 2nd ICST 2017 The existence of this plant height difference is thought to be due to the role of

biocompos fermented with Trichoderma spp fungus, ie: biocompos doses containing T.

koningii isolates Endo-02 and T. harzianum isolates Sapro-07 can inhibit the development of

wilt disease in soybean plants because both this fungus acts as an antagonist that can suppress

the fungus of F. oxysporum. Some earlier researchers such as Sudantha (2007) reported that

invitro fungal Trichoderma spp. effectively inhibits the growth of fungus F. oxysporum f. sp.

vanillae physically (space competition and mikoparasit) and removing antibiotics. Similar

results were reported by Abd-El Moity and Shatla (1981), Trichoderma spp. is a mikoparasit

that can penetrate to the mycelium and sclerotia fungi S. rolfsii resulting in lysis and. Further

Papavizas (1985) states that the mechanism of mycoparasitism begins with the softening of

host cells by enzymes produced by micoparasites before the damage and death of host cells.

According to Hadar et al. (1979), the fungus T. harzianum produces extra-cellular enzymes

ß- (1,3) glucanase and chitinase capable of damaging R. solani cell walls. Cook and Baker

(1983) say that certain strains of Trichoderma produce viridin antibiotics that can inhibit the

growth of other fungi.

4. Conclusion

The results showed that:

1. Higher doses of biocompost granules form (2.5 - 7.5 g granules / plant) and liquid

(2.5 ml - 7,5 ml liquid / plant) accompanied by giving biochar can increase the height

of soybean.

2. Higher doses of biocompost granules form (2.5 - 7.5 g granules / plant) and liquid

(2.5 ml - 7,5 ml liquid / plant) accompanied by biochar can suppress the occurrence of

wilt disease in soybean.

Based on the results of research that has been implemented it can be suggested things

as follows:

1. Further research on maximal dose use to know the height of plants and the incidence

of Fusarium wilt disease in different soil types, time and place.

449

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Proceeding of 2nd ICST 2017

2. Biocompost granuless with a dose of 7.5 g / plant and biocompost with a dose of 7.5

ml / plant can be considered for use in soybean.

Acknowledgements

Thanks to the Director of Research and Community Service Kemenristekdikti, Rector

of the University of Mataram and Chairman of LPPM Mataram University who has provided

Research Fund Graduate Team Fiscal Year 2017, so this article which is part of the research

Postgraduate Team can be arranged well.

References

Brundrett, M, et al. 1996. Working With Mycorrhizas in Forestry and Agriculture.

International Mycorrhizal Workshop. Kaiping China.

Cook dan Baker, 1983. The Nature and Practice of Biological Control of Plant Pathogens.

The American Phytopathol Society Paul MN. 539 p.

Liderman, R. G., 1988. Mychorrizal interaction with the rhizosphere microflora. The mychorrizosphere effect. Phytopathology. 78(3):366-371. http://www.bumn.go.id/ptpn10/galeri/artikel/pelatihan-pembuatan-dan-perbanyakan-mikoriza-di-ugm/. [Diakses 22 Januari 2016].

Mosse, B. 1981. Vesicular abuscular mycorrhiza research for tropical agriculture. Res. Bull.

Hawaii Inst. Trop. Agric. And Human ReSumbers. 82 hal.

Sasli, I., 2004. The Role of Mycorrhizal Vesicles Arbuscular (MVA) in Increasing Plant Resistance Against Drought Stress. Bogor Agricultural University Press. Bogor.

Salisbury, F. B. and C. W. Ross, 1995. Plant Physiology Volume 3. Development plants and plant physiology (Translation D. R. Lukman and Sumaryono). Publisher ITB Bandung.

Setiadi, Y. 2001. The role of arbuscular mycorrhizal in the rehabilitation of critical land in Indonesia. Presented in the Framework of Seminar on the Use of Mycorrhizal Fungi in Organic Farming Systems and Critical Land Rehabilitation.

http://fordamof.org/files/aplikasi_mikoriza_untuk_memacu_pertumbuhan. pdf. [accessed on 22 January 2016].

Sudantha, I. M. 2009. Trichoderma spp Fungi Application (Isolates ENDO-02 and 04 and SAPRO-07 and 09) as Biofungisida, Decomposer and Bioaktivator Growth and Flowering of Vanilla Plant and Its Development on Horticultural and Other Food Crops in NTB. Research Report Grant Competence DP2M Dikti, Mataram.

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Proceeding of 2nd ICST 2017 Sudantha, I. M. 2010. Book of Appropriate Technology: Application of Biofungisida and

Biokompos on Organic Farming. Faculty of Agriculture University of Mataram, Mataram.

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Sudantha, I. M. 2011. Test of Application of Some Types of Biocompos (Fermented Fungi T.

koningii isolate Endo-02 and T. harzianum isolate sapro-07) on Two Soybean Varieties against Fusarium Wilt Diseases and Soybean. Agroteksos No.1: 2.

Windham, M. Y. Elad and R. Baker. 1985. A. Mechanism of increased Plant Growth Induced

by Trichoderma spp. Ohytopathology 76; 518-521.

Xavier L.J.C & Boyetchko S.M. 2004. Arbuscular Mycorrhizal Fungi In Plant Disease Control. In: Arora DK (ed.). Fungal Biotechnology in Agricultural, Food, and Environmental Applications. pp. 183–194 Dekker, New York.

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PROCEEDINGS

The 2nd

International Conference on Science and Technology 2017 “Joint International Conference on Science and Technology in The Tropic”

Mataram, August, 23th

-24th

2017

PUBLISHED BY :

Universitas Mataram

Jl. Majapahit, No 62 Mataram West Nusa Tenggara, Indonesia 83125

Telp: +62-0370-633007

Fax: +62-0370-636041

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PROCEEDINGS

The 2nd

International Conference on Science and Technology 2017 “Joint International Conference on Science and Technology in The Tropic”

Mataram, August, 23th

-24th

2017

Person In Charge : Dr.ret.net. Lalu Rudyat Telly Savalas

Council Committee : Prof. Dr. Ir. H. Lalu Wiresapta Karyadi, M.Si

Head of Steering Committee : Prof. Ir Sunarpi, Ph.D

Deputy of Steering Committee : Prof. Dr. Dato’ Azizan Abu Samah

Peer Reviewer :

Prof. James Gannon, Ph.D Prof. Dr. Mohammad F.R. Hassanien Prof. Dr. Lim Phaik Eem Prof. Yong Hoi Sen Prof. Helmut Erdman Prof. Ir. Sunarpi., Ph.D Prof. Ir. Sri Widuastuti, Ph.D

Prof. I Made Sudarma Prof. I Komang Damar Jaya., Ph.D Dr. Islamul Hadi Dr. rer.nat. Teti Zubaidah, ST., MT I Wayan Sudiarta,.Ph.D Dr. Imam Bachtiar Dr. Syahrul Aluh Nikmatullah., Ph.D

Editor and layout:

Dr.ret.net. Lalu Rudyat Telly Savalas Cahyo Mustiko, ST.,M.Sc., Ph.D Ir. Aluh Nikmatullah, Ph.D Nanda Diniarti, S.Pi., M.Si Dewi Nur’aeni Setyowati, S.Pi, M.Biotech

ISBN : 978 - 602 - 61265 - 1 - 1

Cetakan pertama : Agustus 2017

Published by :

Universitas Mataram Jl. Majapahit, No 62 Mataram West Nusa Tenggara, Indonesia 83125 Telp: +62-0370-633007 / Fax: +62-0370-636041

Copyright law protected Don’t to quote, reproduce and translate some or all of these books without the permission ofthe publisher

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Proceeding of 2nd ICST 2017

PREFACE

Bismillaahirrahmaanirrahiim Assalaamu'alaikumwarahmatullaahwabarakaatuh.

Peace be upon us.

Praise always we pray to God Almighty for giving us the abundance of grace, guidance and inayah, so

that we all can meet here in the “2nd

International Conference on Science and Technology (ICST)

2017”. The theme of this conference is “The Emergence of Science for Human Prosperity and Health” where this conference is joint international conference between Mataram and Malaya University.

First of all, I would like to welcome you all to West Nusa Tenggara Province specially Lombok Island,

“the Island of Thousand Mosques”, which is famous to its many natural resource and beautiful tourism

destinations where you can enjoy them while attending the conference. This conference will be held

for two days, from 23rd

to 24th

August 2017, and took place in campus of the University of Mataram.

So far, we received one hundred fifty papers from various universities and research institutions in

Indonesia and from overseas. The paper have been selected and grouped based on the similarity of the

research field, which then are presented and discussed. Presentation of the papers will be held in seven

parallel classes and poster presentation. The Selected papers will be published in Malaysian Journal of

Science (Special Issue) which index by Scopus, and the rest will be published in the Conference

Proceedings. Additionally, selected paper in aquaculture have the opportunity to be published in Jurnal

Akuakultur Indonesia.

At this moment, the organizing committee would like to express our gratitude to all of you for your

participation on this conference, especially to the all keynote speakers, presenters who have submitted

for both oral and posters presentations and also to all participants. Our special gratitude also goes to

the Rector of the University of Mataram and Vice Chancellor of Malaya University, who have been

highly supporting this conference. Critics and suggestions on the implementation of this conference

will be appreciated and as much as possible we will improve the next ICST. Last but not least, the

organizing committee would like to thank to all of you who have supported this conference.

Have an enjoyable conference. Wassalamu'alaikum warohmatullahi wabarakatuh.

Chairman of 2nd

ICST 2017

Dr.rer.nat. Lalu Rudyat Telly Savalas, M.Si.

i

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Proceeding of 2nd ICST 2017

OPENING SPEECH - RECTOR THE UNIVERSITY OF MATARAM The

2nd

International Conference on Science and Technology 2017 Joint International Conference on Science and Technology in The Tropic Beetwen

Mataram and Malaya Universiti

Respected Guests, Keynote speakers, Conference participants, and all other participants.

On Behalf of all staffs of the University of Mataram, I welcome you all to Lombok, a beautiful island in

West Nusa Tenggara Province, where the University of Mataram is located. Lombok is known for its

natural and cultural diversity where you can enjoy traditional cuisines, beaches, waterfalls, mountain,

traditional villages and handicraft of many ethnics including Sasak, Samawa, Mbojo, Balinese,

Chinese, Arabic, and many others.

As the Rector of the University of Mataram, it is a great honour for me to address the opening of “The

2nd

International Conference on Science and Technology” here at the University of Mataram, which

will be held from 23rd to 24th August 2017, with a theme “The Emergence of Science for Human

Prosperity and Health”. The main aim of this seminar is to gather scientist from all over the world to

share their ideas, knowledge and experiences and to build network for possible future collaboration. As we are aware that sharing knowledge and experiences from speakers are extremely valuable in a

conference, therefore I would like to express my high appreciation, first, to the keynote speakers from

overseas and from Indonesia for their willingness to come to Lombok to share their acknowledged

works. Your effort and contribution to this conference are absolutely valuable. Second, my high

appreciation also goes to the national speakers and all other participants, including the speakers from

University of Mataram and local universities in West Nusa Tenggara Province, your participation in this

conference not only will give incredible share of ideas, skills and knowledge that you have, but also will

improve the academic environment that we are developing in this university. I hope this conference will

be a good forum, not only for communicating and sharing ideas, knowledge and experiences, but also

for building networking for future collaboration.

I would also like to take this opportunity to express my appreciation to the sponsors which have given

some contribution to this conference. Last but not least, I would like to thank the organizing committee

as well as all other supporters and participants, without their effort, commitment and hard work, this

conference will not run well.

Finally, I wish you most successful conference, enjoy Lombok Island and hope to see you again in

other forum here at the University of Mataram.

Rector of the University of Mataram

Prof. Ir. Sunarpi, Ph.D

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Proceeding of 2nd ICST 2017

TABLE OF CONTENTS

Title Preface Opening Speech Table of Contents Agribusiness System in The Agro Tourism Area of Gumantar, North Lombok Zainuri, Taslim Sjah, Ahmad Syauqi, Jayaputra Farming system in Dryland Area of North Lombok

Taslim Sjah1*, I Gusti Lanang Parta Tanaya1, Halil1, Budy Wiryono2

Improving Maize Yield in Tropical Drylands Through Optimum Sunlight Interception by The Plant Canopy

I Komang Damar Jaya1*, Sudirman1, Jayaputra1

Potential of Ant Plant (Myrmecodiapendans) Infuse as an Acute Diarrhea Medicine: An experiment on Rat as a model

Yoni Astuti1*, Idiani Darmawati2, Tantri Wahyu Utami3

Three Dimensional Media and Computer Simulation for The Concept of Heat in Physical Learning

Hikmawati1*, Kosim1, Sutrio1

The Effect of Fractionated Solvent Combination and Sample Concentration on Sun Protection Factor (SPF) Value of Corn Silk Methanol Extract Rosalina Ariesta Laeliocattleya Uniformity Evaluation of Self Compacting Concrete Properties in Beam-Column Structural Elements Using Non-Destructive Testing

Ni Nyoman Kencanawati1*, Akmaluddin1, Hariyadi1, Suryawan Murtiadi1, Hafiz Hamdani1

The Contribution of Soil Management Strategies to Plant and Soil Physical Health, and Soil Microbial Community

Lily Ishak1*, Philip Brown2

Effectiveness of Goat Milk Yogurt Starter Against Various levels of HDL, LDL and Triglycerides in male white rats (Rattus norvegicus Wistar strain)

Sujono1,Wardoyo, I1, Putra,L.Y.1

Adsorption of Free Fatty Acid from Crude Palm Oil on Natural Zeolite Activated with Sodium Hydroxide

Zhilal Shadiq1, Sang Kompiang Wirawan1*, Arief Budiman1

Hypoglycemic Effect of Brucea javanica (L) Merr Leaves and Seed Extract in Alloxan-induced Diabetic Rats

Handa Muliasari1*, Candra Dwipayana Hamdin1, Agus Dwi Ananto1, Muhsinul Ihsan2

Capability Analysis to Regional Innovation Development Based on the Locality Characteristics in Jawa Timur Province Niniek Fajar Puspita1*, Udisubakti Ciptomulyono2, Bambang

Syairudin2, Arya Yudhi Wijaya3

Aquaculture Waste as Ingredients For Cyprinus carpio Feed

Haerudin1*, Zaenal Abidin1*, Ayu Adhita Darmayanti1

Yield and Growth Mungbean (Phaseolus radiates L.) with additional Organic Fertilizer under Intercropping System Eka Widiastuti*, Fitria Zulhaedar dan Lia Hadiawati Desaining And Developing Rechargeable Aluminium-Ion Battery Based On Corncob As Activated Charcoal

Fitriah 1*, Sri Wahyuni1, Aris Doyan2

Urea Recovery from Industrial Wastewater by Adsorption using Porous Carbon and Its Utilization as Fertilizer

iii

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5-11

12-19

20-24

25-31

32-36

37-43

44-50

51-54

55-61

62-67

68-75

76-80

81-91

92-99

100-106

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Proceeding of 2nd ICST 2017

Theresia Evila1*, Agus Prasetya1,2 Teguh Ariyanto, Imam Prasetyo1,2

Imagined Regional Communities: Language Issue in a Decentralisation Policy in Indonesia Ahmad Sirulhaq Magnetotelluric Data Processing Based on Hilbert – Huang Transform

Marenda Dwi Jatmiko1*, Warsa1

Study on Performance and Environmental Impact of Sugarcane-Bagasse Gasification

Shafwan Amrullah1*, Indra Perdana1, Arief Budiman1,2

The Effective Extension Methods for Prevention of Anthrax Diseases on Smallholders Farming in Supproting West Nusa Tenggara Province as A World Halal Tourism Destination Mashur Formula Optimisation of Milk Chocolate Bars Based on the Physical Quality and Organoleptic Characteristics

Dedy Rahmad1*, Muhammad Ikhsan Sulaiman2, Eti Indarti2

Effect of Design Variation of Corncob Biomass Gasification on Boiling Time and Flaming Duration

Joniarta I Wayan1*, Wijana Made1

Activity of Bamboo Rhizobacteria to Inhibit Fusarium oxysporum with In Vitro Screening

Baiq Yunika Hayatin1*, Faturrahman1, Ernin Hidayati1

Isolation and Identification of Chitinolytic Bacteria from Intestinal Tissue or Tilapia (Oreochromis niloticus) Alis Mukhlis1*, Sadikin Amir1, Nurliah1, Bagus Dwi Hari Setyono1, Endang

Wulandari Suryaningtyas2

Structural Behaviour of Spherical Hollow Reinforced Concrete Beam under Flexural Loading

Suryawan Murtiadi1*, Akmaluddin1, Maskimi1

New Record Phoniscusatrox in The Developed Ecotourism Area of South Lombok Islan, West Nusa Tenggara

Siti Rabiatul Fajri1, Sri Nopita Primawati1, Islamul Hadi2, Galuh Tresnani2

The Application of Ground Pumice in Foamed Concrete for Accoustic Material

Maria Asunta Hana Pramudawati1*, Iman Satyarno1 , M. Fauzi Siswanto1

Chemical Compounds Analysis of Green Bean and Its Effect on KopiLuwak Cupping Quality

Murna Muzaifa1*, Dian Hasni1, Anshar Patria 1, Febriani2, Amhar Abubakar3 Quality of Gayo Arabica Coffee Affected by Farm Altitude and Coffee Varieties Yusya

Abubakar1, Dian Hasni1*, Heru Prono Widayat1, Murna Muzaifa1, Mahdi2 Profesional Caring Factor Factors in Providing Services to Clients in Puskesmas Ermina

Istiqomah1*, Dwi Nur Rachmah1, Sudjatmiko Setyobudihono2, Fendy Suhariadi3

Case Report: A-27-years-old female with ST-Elevation Electrocardiography: STEMI or Pericarditis?

Y. Pintaningrum1, S. Alifiya2

Developing of Rechargeable Allumuium-Ion (Al-ion) Battery with Basic Material Activated Coconut Shell Charcoal

S. Wahyuni1*, Fitriah1, A. Doyan1

Simulation of Monthly Rainfall Data of Dodokan Watersheed Using Nonparametric Statistical Downscaling Model

Mustika Hadijati1*, Desy Komalasari1, Nurul Fitriyani1

Simulation of Monthly Rainfall Data of Dodokan Watersheed Using Nonparametric Statistical Downscaling Model

iv

107-111

112-120

121-127

128-135

136-142

143-147

148-152

153-158

159-162

163-165

166-173

174-182

183-189

190-202

203-206

207-212

213-219

220-229

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Proceeding of 2nd ICST 2017

Mustika Hadijati1*, Desy Komalasari1, Nurul Fitriyani1

Enhancement Flexural Strength of Brick Masonry Wall with Polypropylene Bands

Andreas Sugijopranoto1*, Andreas Triwiyono1, Henricus Priyosulistyo1

Utilization of NanoChitosan as Biomordant of Natural Indigo Dye on Cotton Fabrics

Ira Purnawati1*, Yuni Kusumastuti2*, Hary Sulistyo1

Flexural Properties of Tropical Natural Fibres Reinforced Epoxy Composites Prepared Using Vacuum Bagging Method

Jauhar Fajrin1*, Pathurahman1, Suparjo1, Teti Handayani1

The Efficacy of Various Croping Paterns to Utilize a Carry Over Fertilizer Aplication from Tobacco.

Nani Herawati1*, Putu Cakra Putra Adnyana1, Sudarto1, Baiq Nurul Hidayah1

El-Nino Characteristics Based On Reservoir Volumetric

I Wayan Yasa1*, Moh. Bisri2, Moch. Solichin2, Ussy Andawayanti2

Finite Element Modelling of Creep Glued-Laminated Bamboo

Ngudiyono1*,2, Bambang Suhendro3, Ali Awaludin3, Andreas Triwiyono3

Physicochemical and Sensory Properties of Chicken Stock Powder Added with Carrots Extract (Daucus carota, L.)

Ismed*, Aisman, Suci Eka Amalia Growth, Yield and Seed Quality of Corn (Zea mays L.) due to Leaf Defoliation After Tassel ini Dry Land of Lombok Island Indonesia

Hiryana Windiyani1,2*, Eko Widaryanto2, Titiek Islami2

Folate Content In Spinach (Amaranthus sp.), Katuk Leaves (Sauropus androgynous, (L.) Merr) and Singgalang Radish (Brassica oleracea) After Being Processed

Cesar Welya Refdi1*, Fauzan Azima1, Prima Yaumil Fajri2

The Effect of Various Macroalgaes Extract in Lombok to Mortality of Artemia salina Larvae Novita Hidayatun Nufus1, Mursal Ghazali2, Rina Kurnianingsih2, Aluh

Nikmatullah3, Sunarpi2*

Tuned Mass Damper as Alternative Solution for Pedestrian Comforet in Existing Truss Bridge

Yumna Cici Olyvia1*, Bambang Suhendro1, Ashar Saputra1

Children's Playground and Safety at the Kahayan Riverbank Settlement, Palangkaraya Parmonangan Manurung The Potency of Kersen Leaves (Muntingia calabura Linn.) to Decrease High Blood Sugar Level

Ihlana Nairfana1*, I Ketut Suter2

Subsurface Structure Interpretation of Southern Flank of Tangkuban Parahu Based on Gravity Method

Hasdi Abdullah1*, Djoko Santoso2

The Importance of Early Maturing Hybrid Maize in The Limited Rainfall Areas to Anticipate Climate Changes

Baiq Tri Ratna Erawati1*, Awaludin Hipi1, Andi Takdir M.2

A Neural Network Model for Indonesia’s Foreign Reserves Model Syamsul Bahri The Use of Near Infrared Technology for Rapid Measurement of Soil Nitrogen in Rice Paddy Field of Lombok Island Bambang Hari Kusumo Sea Horse (Hippocampus sp.) Broodstock Culture in Controlled Tank

Dewi Nur’aeni Setyowati1*, Nanda Diniarti1, Nunik Cokrowati1

v

230-236

237-242

243-249

250-256

257-262

263-268

269-275

276-282

283-286

287-290

291-298

299-304

305-308

309-318

319-324

325-331

332-336

337-339

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Proceeding of 2nd ICST 2017

Analysis on Heat Energy Utilization on White Copra Drying using Solar Energy Tray

Drier Murad1*, Rahmat Sabani1, Guyup Mahardhian Dwi Putra1, Diah Ajeng Setiawati1

Application of Hydrological Water Supply Model to Calculate Discharge of Maronggek River in East Lombok Sirajuddin Haji Abdullah1*, Hera Damayanti1, Guyup Mahardhian Dwi Putra1 , Diah

Ajeng Setiawati1

Utilization of Ulva lactuca to Increase The Growth of Eucheuma cottonii Nunik Cokrowati Sociotechnical Adaptive Water Governance: A Case Study of Water Governnce in Lombok Indonesia

Alex Laplaza1*, I Gusti L.P. Tanaya2, Suwardji3

Characterization of Rice Husk and Wood Biochars and Their Effect on Soil Chemical and Agronomic Properties of Lettuce (Lactuca sativa L.)

Mulyati*, Tejowulan, S., Baharuddin, AB., Sukartono, Suwardji Seagrass Ecosystems Monitoring as Related to Coral Reef in Coastal Waters of Sekotong, West Lombok, Indonesia

Abdul Syukur1*, Didik Santoso1

Fatty acid Composition of Ethanolic Extract of Seahorse (Hippocampus barbouri) from Ekas Bay, Lombok Island, West Nusa Tenggara

Seto Priyambodo1*, Dewi Nur’aeni Setyowati2, Nunik Cokrowati2, Nanda Diniarti2

Effectiveness of Ulva lactuca Extract on Kappaphycus alvarezii Growth

Muhammad Fadlillah1*, Nunik Cokrowati1, M. Masyarul Rusdani Composition of Plankton on Floating Area in Batunampar Beach, East lombok Nanda Diniarti Water quality of Kertasari Bay West Sumbawa as Sustainability of Seaweed Cultivation Center

Edi Sulman1, Nunik Cokrowati2, Arziahningsih1, Rinto Basuki1

Mapping Consumer Preferences and Physical Quality of Sie Reuboh (Cooked Meat) a Traditional Cuisine of Aceh Dian Hasni, Novia Mehra Erfiza, Muhammad Faiz, Ulva Syahrina Analysis of The Role of Midwives and Nutritionists in Early Detection of Malnutrition and Growth Disorder in Two Year Old Babies at Narmada Public Health Care Working Area

Lina Nurbaiti1*, Lalu Bramawangsa Banjar Getas1*, Sandra Yuliana Andini Putri1*,

Felix Santoso1*, Ni Putu Ayu Dewanthi1*, Amalia Asfarina1*

Correlation betweenBCL-2 and LMP-1 expression in patients with Nasopharyngeal Carcinoma WHO Type III

Hamsu Kadriyan1, Didit Yudhanto1, Fathul Djannah2,I Gusti Ayu Trisa Aryani3,

Muhammad Alfian3, Markus Rambu3, Muhammad Rizqi Kholifaturrohmy1

340-347

348-357

358-363

364-376

377-384

385-392

393-397

398-404

405-410

411-413

414-421

422-426

427-431

Influence of Arbuscular Fungi Mycorrhiza and Dose Bioactivator (Tablet and Liquid Form) Fermented with Trichoderma spp. Against Growth and Wilt Disease on Soybean Siwi Yudhiarti1)*, I Made Sudantha2), M. Taufik Fauzi2)

432-441

Influence of Coconut Shell Biochar and Dose Biocompost (Granules and Liquid Form)

Fermented with Trichoderma spp. Against Growth and Wilt Disease on Soybean

U’ul Efriyanti Prayoba1)*, I Made Sudantha2), Suwardji2)

442-451

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