process model for her2 signaling pathways
TRANSCRIPT
Process model for HER2 Signaling Pathways Student: Vishakha Sharma1, Yifei Bao1,
Advisor: Adriana B. Compagnoni1, Joseph S. Glavy2 Department of Computer Science1, Department of Chemistry, Chemical Biology and Biomedical Engineering2,
Schaefer School of Engineering and Science, Stevens InsJtute of Technology, Hoboken, NJ 07030
IntroducJon • HER2 is a cell membrane surface-‐bound
receptor tyrosine kinase and is involved in signal transducJon pathways leading to cell growth and differenJaJon.
• HER2 is a protein responsible of higher aggressiveness in breast cancers.
HER2 Signaling Pathways • AKT/PI3K Pathway • ERK Pathway • STAT/IL6 Pathway
Results • DifferenJal HER2 concentraJons
a. 100 HER2 are iniJalized b. 30 HER2 are iniJalized • Overview of different species
• Crosstalk of ERK and PI3K pathways
a. ERK Pathways with b. ERK Pathways without
PI3K Pathway PI3K Pathway • InhibiJon of Raf acJvaJon(acJvaJon
rate is changed from 27.0 to 1.0E-‐4 )
Experiment SPiM – StochasJc Pi Machine • SPiM is a programming language used for designing and simulaJng
computer models of biological processes.
• This language is based on a mathemaJcal formalism known as pi-‐calculus and simulaJon algorithm is based on standard kineJc theory of physical chemistry.
• It features a simple graphical notaJon for modeling a range of biological and can be used to model large systems incrementally, by directly composing simpler models of subsystems.
SPiM Model for AKT/PI3K Pathway
SPiM Model for ERK Pathway
SPiM Model for STAT/IL6 Pathway
Conclusion • Using a process model simulaJon, we can know the effect of the differenJal concentraJons of components and we can research on the crosstalk between different signal transducJon pathways.
• We can also simulate the inhibiJon on specific reacJons in the system.
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