proposal mpba kelompok 4 flavonoid
TRANSCRIPT
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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PRAKTIKUM METODE PEMISAHAN BAHAN ALAM
PEMISAHAN, ISOLASI, DAN IDENTIFIKASI FLAVONOID KUERSETIN
DARI TANAMAN MAHKOTA DEWA (PHALERIA MACROCARPA)
KELOMPOK PRAKTIKUM: IV
HARI/TANGGAL PRAKTIKUM:
KAMIS/ 02 SEPTEMBER-11 DESEMBER 2014
DISUSUN OLEH:
M!" P#$% A!$&''# 110*0*0+0
"#$.$" P$ 110*0*0+2
N3% T#$ W$#$.$ 110*0*0++
A A' I.#$ S$. I!#$ 110*0*0+
I! A' M!" K"'% D"5$ 110*0*0+
LABORATORIUM FARMAKOGNOSI DAN FITOKIMIA
JURUSAN FARMASI
FAKULTAS MATEMATIKA DAN ILMU PENGETAHUAN ALAM
2014
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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s$onin+ D#n !)%o' de/ di*$o"%n !en&nd#n& sen(/ *%*oid3 s$onin3 dn
$o*i-eno* *i&nin+ Sedn&%n %#*i' ,#) !en&nd#n& *%*oid3 s$onin3 dn -*8onoid
D*i!"')3 2007+ Knd#n&n -*8onoid (n& 'e"d$' $d %#*i' ,#) !)%o' de/
din'"n( %e!$-e"o*3 !("ie'in3 n"in&in3 dn "#'in+ Sedn&%n %nd#n&n n"in&in
dn ?#e"e'in 'e"d$' $d d&in& ,#) !eso%"$ d"i !)%o' de/ Hend" et al.,
2011+ Pene*i'in (n& di*%#%n o*e) Hend" et al. (2011 !en('%n ,)/ sen(/
-*8onoid (n& 'e"%nd#n& d*! ,#) !)%o' de/ Phaleria macrocarpa !e!i*i%i
%'i8i's 'in&&i se,&i n'io%sidn+ Se*in i'#3 'n!n !)%o' de/ 6#& di*$o"%n
!e!i*i%i %'i8i's se,&i n'i,%'e"i3 dn n'i in-*!si Ao et al+3 200=+ Be,e"$
sen(/ -*8onoid d"i !)%o' de/ se$e"'i %#e"se'in !e!i*i%i %'i8i's 'in&&i
se,&i n'i,%'e"i d*! !e!,#n#) ,%'e"i $')o&en den&n !e"#s% sin'esis DNA
d"i ,%'e"i $')o&en 'e"se,#' Hend" et al.,2011+
Be"ds"%n in-o"!si di's3 'e*) di%e')#i sen(/ -*8onoid d*! 'n!n
!)%o' de/ Phaleria macrocarpa !e!i*i%i $o'ensi (n& ,es" se,&i ,)n o,'+
Bn(%n( %nd#n&n %i!i (n& 'e"d$' $d 'n!n !)%o' de/ Phaleria
macrocarpa !e!,#' $"%'i%n 'e"'"i% #n'#% !en&iso*si sen(/ -*8onoid (n&
'e"%nd#n& did*!n(+ Hsi* iso*si sen(/ -*8onoid ini di)"$%n d$'
din%n se,&i #n d*! $"oses iso*si sen(/ -*8onoid d"i ,#) !)%o'
de/ Phaleria macrocarpa+
1626 R'%' M&
D"i *'" ,e*%n& di 's di$e"o*e) $e"!s*)n ,&i!n " !en&iso*si
sen(/ F*8onoid (n& 'e"d$' d*! ,#) !)%o' de/ Phaleria macrocarpa@
16+6 T'7' P#.$'%
T#6#n d"i $"%'i%#! ini d*) #n'#% !en&iso*si dn iden'i-i%si iden'i-i%si
%"%'e"issisen(/ -*8onoid d"i ,#) !)%o' de/ Phaleria macrocarpa+
1646 I38$ B#' M3!$9$$ S"!"# D$.%;$&
Pd $"%'i%#! ini di*%#%n ino8si $d !e'ode $e!#"nin+ Me'ode
$e!#"nin (n& din%n $d 6#"n* #n d*) %"is'*issi+ Ino8si ,"# (n&
di*%#%n s' $e!#"nin sen(/ (i'# di&n'i !en&n%n K"o!'o&"-i L$is
Ti$is P"e$"'i- KLTP+ Pe"'i!,n&n !en&n%n K"o!'o&"-i L$is Ti$is
P"e$"'i- di,ndin&%n !en&n%n !e'ode "e%"is'*issi d*) $e*"#' (n&
din%n *e,i) sedi%i' di,ndin&%n !en&n%n !e'ode %"is'*issi di,#'#)%n
$e*"#' (n& *e,i) ,n(%3 se"' di*i)' d"i e-isiensi /%'#3 den&n !en&n%n
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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!e'ode KLT P"e$"'i- *e,i) e$' di,ndin&%n !en&n%n !e'ode "e%"is'*issi
di,#'#)%n /%'# (n& *!+Modi-i%si sede")n (n& di*%#%n@@@
7
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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BAB II
TIN
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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'# ,#*' 'e*#"3 $n6n& 4. !3 di!e'e" 7. !3 $e"!#%n *iin3 ,e"*#"3
,e"/"n !e") 6i% !'n& dn ,e"/"n %e)i6#n s' ,e*#! !'n&+ Bi6i
,#*'3 %e"s3 /"n o%*'+ A%" '#n&&n&3 %#nin& %eo%*'n Sdi%#n3 et.al.,
2017+
2616+6K!' ! K$. T%
Be",&i %nd#n&n %i!i 'e*) di'e!#%n d*! 'n!nP. macrocarpa
den&n %onsen'"si (n& ,e",ed.,ed din'"n( !)%oside A3 s!
dode%noi%3 s! $*!i'i%3 des.e'(* -*8io"din.A3 -*8io"din.A3 -*8io"din.
D3 -*8io"din.A &*#%osid3 e'i* s'e"'3 *i&nns dn s#%"os+ Pd ,&in ,'n&
dn ,#)n( %( %n %nd#n&n s$onin3 *%*ois3 $o*i-eno*3 -eno*3 -*8noid3
*i&nns dn 'nnin+ Sedn&%n $d ,&in $e"i%$ d"i ,#) 'e"d$' sen(/.
sen(/ -*8onoid se$e"'i %e!$-e"o*3 !("ie'in3 n"in&in dn "#'in+ Un'#%
sen(/ N"in&in dn ?#e"i'in d$' di'e!#%n 6#& $d ,&in !eso%$ dn
,i6i d"i !)%o' de/ Sdi%#n3 et.al., 2017+
G!," 2+ Knd#n&n ,io%'i- (n& diiso*si d"i e%s'"%Phaleria macrocarpa:
-e8io"din.A3 , -e8io"din.D3 !&ni-e"in3 d %e!$-e"o*3 e !("ie'in3 -
n"in&in3 & &**i id3 ) "#'in3 i ?#e"i'in3 6 *"ii"esino*3 % $ino"esino*3
*!'i"esino* Sdi%#n3 e'+*+3 2017
1
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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Pd $ene*i'in se,e*#!n(3 !e',o*i' se%#nde" d"iP. macrocarpa,e"#$
Ke!$-e"o*.7.O..D.&*#%osid (n& 'e"d$' d*! ,#) 'e",#%'i d$'
!e*ind#n&i )e$'o! H4IIE 'i%#s 'e")d$ s'"es o%sid'i-+ Se*in i'#3 di$e"o*e)
)si* iso*si d"i 'i& sen(/ i"iside 973 -*e"in3 dn Mn&i-e"in (n& ,e"s*
d"i ,#) !)%o' de/+ Di!n i"iside 973 !en#n6#%%n %'i8i's
8so"e*%sn *!,' 'e")d$ %on'"%si ind#%si no"d"en*in di*i)' d"i o"'
'i%#s Hend" et al.,2011+
G!," 7+ Po*i%ons'i'#en (n& diiso*si d"iPhaleria macrocarpa
Se" e!$i"is3 di(%ini ,)/ !)%o' de/ !e!i*i%i $o'ensi #n'#%
!en&o,'i )i$e"'ensi3 di,e'es3 %n%e" dn se,&i di#"e'i%+ Sedn&%n3 se"
phytochemical 'e*) di*$o"%n3 !)%o' de/ !e!i*i%i ,e",&i %'i8i's
,io*o&is 'e"!s#% n'io%sidn3 n'i!i%"o, dn n'i.in-*!si+ E%s'"% !e'no*
d"i ,#) Phaleria macrocarpa sn&' ,i% se,& n'i!i%"o,+ Me%nis!e
-*8onoid n'i!i%"o,n( d$' di%*si-i%si%n se,&i $en&)!,' sin'esis
s! n#%*e'3 !en&&n& -#n&si !e!,"n si'o$*s!3 dn !en&)!,'
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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!e',o*is!e ene"&i Hend" et al., 2011+ D*! s'#di *inn(3 di%'%n
%nd#n&n -*8onoid se$e"'i "#'in se" #!#! !e!i*i%i %'i8i's se,&i n'i
in-*!si3 dn n'i)i$e"&*i%e!i+ Sedn&%n Ke!$-e"o*s !e!i*i%i %'i8i's
se,&i n'i,%'e"i 'e")d$ S. aureus, Enterococcus faecalis, Escherichia coli
dnP. aeroginosa. Ke!$-e"o* 6#& !e!i*i%i %'i8i's d*! !en#"#n%n "esi%o
$en(%i' %"dio8s%#*" (n& se" sine"&is ,e%e"6 den&n i"iside se,&i
8so"e*%sn dn !en#"#n%n 'e%nn d") Sdi%#n3 et.al., 2017+ K#e"se'in
!e!i*i%i %'i8i's 'in&&i se,&i n'i,%'e"i d*! !e!,#n#) ,%'e"i $')o&en
den&n !e"#s% sin'esis DNA d"i ,%'e"i $')o&en 'e"se,#' Hend" et al.,2011
2626 M".3!" E.#$
262616M"#$
E%s'"%si !e"#$%n %e&i'n $en"i%n >' (n& d$' *"#' d"i ,)n
(n& 'id% d$' *"#' den&n $e*"#' i"+ P"oses (n& 'e"6di se*! e%s'"%si
di!#*i d"i %on'% $e*"#' den&n dindin& se* '#!,#)n3 $ene'"si $e*"#' %e
d*! se* '#!,#)n3 $e*"#'n >' %'i- d*! se*3 di-#si >' %'i- %e *#" se*3 dn
$en!$#*n >' %'i- (n& 'e*) 'e"e%s'"%si S'i)e"3 200=+
Mse"si !e"#$%n $"oses e%s'"%si den&n " din&in+ Me'ode !se"si
di*%#%n den&n !e"end! se",#% si!$*isi den&n $e*"#' (n& ses#i se*!
,e,e"$ )"i $d 'e!$e"'#" %!" Hnd3 200=+ P"oses (n& 'e"6di d*)
i"n $en("i %n !ene!,#s dindin& se* '# !s#% %e d*! "on&& se* (n&
!en&nd#n& >' %'i-3 >' %'i- 'e"se,#' %n *"#' %"en dn( $e",edn
%onsen'"si n'" *"#'n >' %'i- di d*! se* den&n (n& di *#" se*+ L"#'n
(n& *e,i) $e%' (n& ,e"d di d*! se* dides% %e*#" se*3 !s#% %e d*!
*"#'n di *#" se*+ Pe"is'i/ 'e"se,#' ,e"#*n& se)in&& 'e"6di %ese'i!,n&n%onsen'"si n'" *"#'n di *#" se* dn di d*! se*+ P"oses !se"si %n *e,i)
o$'i!* 6i% di*%#%n $en&d#%n den&n %oo%n $d /d) '#
!en&n%n !&ne'i% s'i"e" dn $e*"#' (n& din%n )"#s di$e",)"#i
)in&& "ende!en (n& di$e"o*e) *e,i) ,n(% 9)nne*3 1
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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E%s'"%si i".i" din%n se,&i " #n'#% $" $e"*%#n s!$e* '#
clean-up s!$e* #n'#% !e!is)%n n*i'.n*i' d"i %o!$onen ;%o!$onen
!'"i%s (n& !#n&%in !en&&n& $d s' %#n'i-i%si '# de'e%si n*i'
Gnd6" dn Ro)!n3 200+ Di!n3 e%s'"%si i" ; i" sendi"i d*) s#'#
$"oses $e!is)n s#,s'nsi d"i !$#"nn( den&n !en&n%n $e*"#' (n&
ses#i di!n s#,s'nsi (n& die%s'"%si 'e"d$' d*! !$#"nn( (n&
,e",en'#% i" K"is'n'i d%%3 200=+ Pe*"#' (n& din%n d*) $e*"#'
o"&ni% (n& !e!$#n(i %e*"#'n "end) d*! i" C103 d$' !en$
se)in&& !e!#d)%n $en&)i*n&n $e*"#' se'e*) di*%#%n e%s'"%si3 dn
!e!$#n(i %e!#"nin (n& 'in&&i #n'#% !e!ini!*%n dn( %on'!insi
s!$e* !is*n(3 i": !e'no* dn ,#'no* dn dn( $en!,)n H9L 10+
Fse i" ,e"d di,/)3 %"en ,e"' 6enis d"i i" *e,i) ,es" di,ndin& ,#'no*+
Be"' 6enis i" 03
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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K"o!'o&"-i *$is 'i$is KLT !e"#$%n s*) s'# 'e%ni% (n& se"in&
din%n d*! !en&iden'i-i%si s#'# n*i' d*! n*isis 'o%si%o*o&i dn
din%n se" *#s d*! $e!is)n dn iden'i-i%si o,' %"en 'e%ni% ini
e$'3 !en&)si*%n )si* den&n "e,i*i's (n& 'in&&i3 dn !e!e"*#%n sedi%i'
,i( Mo--' et al.3 200+
P"!e'e" $d KLT (n& din%n #n'#% iden'i-i%si d*) ni*i R-+ R-
di%"%'e"issi den&n 6"% !i&"si so*#' 'e")d$ 6"% #6#n& -se &e"%n(+
F%'o" "e'"dsi so*#' R- dide-inisi%n se,&i:
Ni*i !%si!#! R- d*) 1 dn ini di$i %e'i% so*#' !e!$#n(i
$e",ndin&n dis'"i,#si D dn -%'o" "e'ensi % s! den&n 0 (n& ,e""'i
so*#' ,e"!i&"si den&n %ee$'n (n& s! den&n -se &e"%+ Ni*i !ini!#!
R-3 d*) 0 dn ini 'e"!'i 6i% so*#' 'e"')n $d $osisi 'i'i% /* di
$e"!#%n -se di! Gnd6" dn Ro)!n3 200+
26+646K#3%.3#9$ V'% $#
T#6#n d"i %"o!'o&"-i 8%#! i" d*) #n'#% !e!is)%n %o!$onen
sen(/ (n& 'e"%nd#n& d*! s#'# e%s'"% %ed*! ,e,e"$ -"%si,e"ds"%n %e$o*"n K#s!"di(ni dn N//i3 1
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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'e'$i 'id% oo% #n'#% %o!$onen sen(/ (n& $o*" %"en sen(/ 'e"se,#'
%n dii%' %#' o*e) dso",en K#s!"di(ni dn N//i3 1
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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)id"o%si* !en&)si*%n $e"&ese"n )i$so%"o!i% !en&%i,'%n M"%)!3
1
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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se'on3 se",#% )*#s s! ,o"'3 se",#% )*#s s! o%s*'3 e'e"3 *"#'n ,esi III
%*o"id3 s! se'' n)id"'3 s! s#*-'3 %*o"o-o"!3 ?#des'3 n.)e%sn3 e'i* se''3
$*' KLT si*i% &e* 0 GF243 !oni3 NOH3 A*9*7+
+62 P#3"!'# P""&$.$
+6261 P"$; B
B#) !)%o' de/ (n& !si) se&" di#i s!$i ,e"si) %e!#din dii"is 'i$is
dn di%e"in&%n den&n " din&in.n&in%n+ B#) (n& 'e*) %e"in& %e!#din
di,*ende" )in&& !en6di se",#%+ Se",#% %e"in& %e!#din disi!$n di /d) (n&
'e"*ind#n& )(+
+6262 E.#$ ! P"'; P"'.
Se,n(% 0 &"! se",#% ,#) !)%o' de/ di!se"si den&n 20 !L
!e'no*se*! 1 )"i s!,i* did#% sese%*i+ Se*n6#'n(3 !$#"n 'e"se,#' dis"in&+
Fi*'"' disi!$n dn !$s di"e!se"si den&n 200 !L !e'no*se*! 1 )"i s!,i*
did#% sese%*i+ 9!$#"n %e!#din dis"in& dn -i*'"' di%#!$#*%n+ A!$s
di"e!se"si %e!,*i den&n 200 !L !e'no* se*! 1 )"i s!,i* did#% sese%*i+
9!$#"n %e!#din dis"in& dn se!# -i*'"' di%#!$#*%n+ Mse"' di$e%'%n
den&n rotary evaporator$d s#)# 409 se)in&& di$e"o*e) e%s'"% %en'* !e'no*+
+626+ S#$$ F$.3$%$ E.#
. P"%='. L#'. U7$
Pe!,#'n *"#'n #6i #n'#% s%"inin& -i'o%i!i di*%#%n den&n !e*"#'%n
70 !& e%s'"% !e'no* ,#) !)%o' de/ d*! 70 !L !e'no*+
. U7$ A&&3$!
Se,n(% 2 !L *"#'n #6i di#$%n di's /n $o"se*en )in&& di$e"o*e)
"esid#+ Resid# %e!#din di*"#'%n den&n !L H9* 2N+ L"#'n (n& did$'
%e!#din di,&i %e d*! 4 ',#n& "e%si+ T,#n& $e"'! di'!,)%n s!ene" (n& ,e"-#n&si se,&i ,*n%o3 $d ',#n& %ed# di'!,)%n 7 'e'es
$e"e%si D"&end"o--5 $d ',#n& %e'i& di'!,)%n 7 'e'es $e"e%si M(e"5
dn $d ',#n& %ee!$' di'!,)%n $e"e%si &ne"+ Te",en'#%n( end$n
6in&& $d ',#n& %ed#3 end$n %#nin& $d ',#n& %e'i& dn end$n
o%*' $d ',#n& %ee!$' !en#n6#%%n dn( *%*oid Pd!s"i d%%+3
2017+
. U7$ F&833$!
=
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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Se,n(% 1 !L *"#'n #6i di#$%n )in&& %e"in&3 sisn( di,s)%n
den&n se'on P3 di'!,)%n sedi%i' se",#% )*#s s! ,o"' P3 dn se",#% )*#s
s! o%s*' P3 di$ns%n )'i.)'i di 's $enn&s i" dn di)ind"i $e!nsn
,e"*e,i)n+ Sis (n& di$e"o*e) di!$#" den&n 10 !L e'e" P+ Di!'i den&n
sin" U 7 n!5 *"#'n ,e"-*#o"esensi %#nin& in'ensi- !en#n6#%%n dn(
sen(/ -*8onoid Pd!s"i d%%+3 2017+
. U7$ T$
Se,n(% 1 !L *"#'n e%s'"% #6i di"e%si%n den&n *"#'n ,esi III
%*o"id 10+ Adn( 'nin di'#n6#%%n den&n 'e",en'#%n( /"n ,i"# '# '#
)i'! %e)i6#n Pd!s"i d%%+3 2017+
. U7$ G&$3$!
Se,n(% 031 !L *"#'n #6i di#$%n di 's $enn&s i"+ Resid# %e!#din
di*"#'%n d*! !L s! se'' n)id"' P dn di'!,)%n 10 'e'es s!
s#*-' P+ 'e",en'#%n( /"n ,i"# '# )i6# !en#n6#%%n dn( &*i%osid
"e%si Lie,e"!nn.B#")"d Pd!s"i d%%+3 2017+
. U7$ S;3$
Se,n(% 10 !L *"#'n e%s'"% #6i d*! ',#n& "e%si di%oo% 8e"'i%*
se*! 10 de'i%3 %e!#din di,i"%n se*! 10 de'i%+ A!'i %e6din
'e",en'#%n( ,#s+ Pe!,en'#%n ,#s se'in&&i 1 ! ; 10 ! (n& s',i* se*!
'id% %#"n& d"i 10 !eni'3 !en#n6#%%n dn( s$onin+ Pd $en!,)n 1
'e'es H9L 2N3 ,#s 'id% )i*n& Pd!s"i d%%+3 2017+
. U7$ S."#3$! ! T#$."#;"3$!
Pe!e"i%sn s'e"oid dn 'e"$enoid di*%#%n den&n "e%si Lie,e"!nn.
B#")"d+ Se,n(% 2 !L *"#'n #6i di#$%n d*! /n $o"se*en+ Resid#
di*"#'%n den&n 03 !L %*o"o-o"!3 %e!#din di'!,)%n 03 !L s! se''n)id"'+ Se*n6#'n( di'!,)%n 2 !L s! s#*-' $e%' !e**#i dindin&
',#n&+ A$,i* 'e",en'#%n( inin %eo%*'n '# 8io*e' $d $e",'sn
*"#'n !en#n6#%%n dn( 'e"$enoid3 sedn&%n ,i* !#n#* inin ,i"#
%e)i6#n !en#n6#%%n dn( s'e"oid Pd!s"i d%%+3 2017+
. U7$ M$ A.$#$
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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Se,n(% 1 !L *"#'n #6i di#$%n di's /n $o"se*en )in&& di$e"o*e)
"esid#+ Hsi* $osi'i- !in(% 'si"i di'ndi den&n "o! %)s di)si*%n o*e)
"esid# 'e"se,#' Pd!s"i d%%+3 2017+
+6264 F#$$ E.# (E.#$ $#-$#) ! P"'; F#$
Se,n(% 10 &"! e%s'"% %en'* !e'no* )si* e%s'"%si di*"#'%n den&n 70
!L H9* 13 %e!#din di'!,)%n den&n 10 !L n.)e%sn dn di!s#%%n %e d*!
o"on& $is)+ 9!$#"n 'e"se,#' %e!#din di&o6o&3 se)in&& di$e"o*e) -"%si ,e"i"
s! dn -"%si n.)e%sn+ Ln&%) 'e"se,#' di*%#%n se,n(% 'i& %*i+ Se!#
-"%si n.)e%sn %e!#din di'!$#n& dn -"%si ,e"i" (n& di$e"o*e) %e!#din
di$"'isi %e!,*i den&n 10 !L e'i* se''+ P"'isi den&n e'i* se'' 6#& di*%#%nse,n(% 'i& %*i+ Msin&.!sin& -"%si -"%si ,e"i"3 -"%si n.)e%sn3 dn -"%si e'i*
se'' di#$%n $e*"#'n(+ Se!# -"%si %en'* (n& di$e"o*e) di'i!,n&+
+626* K#3%.3#9$ L;$ T$;$ (KLT) H$& F#$$
+ Pen(i$n P*'
Disi$%n $*' KLT Si*i% Ge* GF24den&n #%#"n 410 !3 %e!#din $d
,&in 's dn ,/) di,e"i ,'s 1 !+ P*' %e!#din di#i d*! chamber
(n& ,e"isi 10 !L !e'no* dn die*#si s!$i se!# $*' 'e",s)i+ P*'
%e!#din di%'i8si d*! o8en $d s#)# 1109 se*! 1 !eni'+
,+ Pen(i$n E*#en
Di,#' e*#en (n& 'e"di"i d"i %*o"o-o"!:se'on:!e'no* :13:13 88
se,n(% 10 !L+ Di$i$e' %*o"o-o"! se,n(% !L3 se'on se,n(% 13 !L dn
!e'no* se,n(% 13 !L %e d*! *,# #%#" 10 !L %e!#din di&o6o& )in&&
)o!o&en+ Chamber%e!#din di6en#)%n den&n e*#en (n& 'e*) di,#'++ Peno'o*n dn Pen&e*#sin
F"%si ,e"i"3 n.)e%sn3 e'i* se''3 dn e%s'"% )si* !se"si !sin&.!sin&
di'i!,n& se,n(% 10 !& dn di*"#'%n den&n 1 !L !e'no*+ Se*n6#'n(
!sin&.!sin& -"%si di'o'o*%n se,n(% L $d $*' KLT den&n 6"% 1 !
n'" 'o'o*n+ Ke!#din $*' die*#si den&n e*#en d*! chamber s!$i ,'s
's $*'+
d+ Iden'i-i%si
P*' (n& 'e*) die*#si3 di!'i s$o'.s$o' (n& 'e",en'#% $d $*' di ,/)
*!$# U den&n $n6n& &e*o!,n& 7 n!+ S$o' !en&nd#n& -*8onoid
10
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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$,i* s$o' ,e"/"n %#nin&.o"n(e+ P*' 'e"se,#' %e!#din di$$"%n #$
!oni dn di*i)' %e!,*i di ,/) sin" U 7 n!+ S$o' !en&nd#n&
-*8onoid di'ndi den&n sedi%i' '# 'n$ $e"#,)n /"n+
+626> S'=9#$$ (K#3%.3#9$ K3&3% V'% $#)
Iso*si s%o$o*e'in %#e"se'ind"i ,#) !en&%#d# !)%o' de/ di*%#%n
den&n !e'odeVacuum Liui! Chromatograph( L9 !en&n%n si*i% &e*
0 se,&i -se di! dn n.)e%sn3 e'i* se''3 dn !e'no* se,&i -s &e"%
,e"ds"%n 'e%ni% "Step #ra!ient Polarit( SGP+ F"%si e'i* se''
di'!,)%n sedi%i' n.)e%sn!e'no* )in&& 'e"*"#' %e!#din di'!,)%n
si*i% s! ,n(% den&n ,e"' -"%si e'i* se''%*o"o-o"!1:1 #n'#% di,#'
!en6di se",#% $"edso",si+ Pe*"#'n( di#$%n se" in vacuo sSe)in&&
di$e"o*e) !$#"n si*i% &e* dn s!$e* ,e"#$ ,#,#% %e"in&+ Ko*o! di,#'
den&n!e*$isi %o*o! G.7 !en&n%n %e"'s s"in& $d ,&in ,/)n(
&" se",#% si*i% 'id% 6'#) d"i o"on& G7+ Se*n6#'n( diisi dso",en si*i% &e*
0 s!$i se'in&&i 23 ! 10 &"!3 %e!#din di%e'#%.%e'#% den&n '#6#n &"
'in&&i d"i dso",en d*! %o*o! "' $d se*#"#) sisi se"' !en"n&i dn(
"on&& #d" $d %o*o! se)in&& %o*o! !en6di *e,i) %o!$%+ Se'e*) se",#%
si*i% G0 di'#n& %ed*! o"on&3 %e!#din di's o"on& di*e'%%n %e"'s
s"in& den&n '#6#n #n'#% !e*ind#n&i se",#% si*i% %e'i% $en#n&n -se &e"%+
Se,e*#! di*%#%n e*#si3 %o*o! die%#i*i,"si 'e"*e,i) d)#*# den&n n.)e%sn
#n'#% !e!,s)%n si*i%3 !e!d'%n dn !e!s'i%n ,)/ %o*o! (n&
di)si*%n 'e*) %o!$% se)in&& $"oses e*#si den&n !en&n%n $e*"#'
,e"i%#'n( d$' ,e"6*n den&n ,i%+ !e!s#%%n 10 &"! si*i% (n& 'e*)
di'!,)%n $e*"#'n.)e%sn %e d*! %o*o! s!,i* di%e'o%.%e'o% )in&& $d'
dn )o!o&en+ Se'e*) si*i% $d'3 ,#,#" $"edso",si di*e'%%n se" )'i.)'i
dn !e"' di 's ,#,#" si*i% (n& 'e*) $d t+ Ko*o! %e!#din die*#si den&n
sis'e! Step #ra!ien Polaritydi!#*i d"i $e*"#' n.)e%sn3 n;)e%sn: e'i* se''3
e'i* se'':!e'no*3 dn !e'no* s6 (n& %e$o*"nn( di'in&%'%n se"
,e"')$ !e**#i ,e,e"$ $e",ndin&n se)in&& di$e"o*e) $e!is)n (n&
,i%+ E*#en di,#' se,n(% 10 !L den&n %o!$osisi se,&i ,e"i%#':
E*#en Ko!$osisi Pe",ndin&n 88
I n.)e%sn:e'i* se'':!e'no* 10:0:0
II n.)e%sn:e'i* se'':!e'no* =:2:0
11
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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III n.)e%sn:e'i* se'':!e'no* :4:0
I n.)e%sn:e'i* se'':!e'no* 4::0
n.)e%sn:e'i* se'':!e'no* 2:=:0
I n.)e%sn:e'i* se'':!e'no* 0:=:2
II n.)e%sn:e'i* se'':!e'no* 0::4III n.)e%sn:e'i* se'':!e'no* 0:4:
I n.)e%sn:e'i* se'':!e'no* 0:2:=
n.)e%sn:e'i* se'':!e'no* 0:0:10
+626 K#3%.3#9$ L;$ T$;$ (KLT) H$& S'=9#$$
+ Pen(i$n P*'
Disi$%n $*' KLT Si*i% Ge* GF24den&n #%#"n 1010 !3 %e!#din
$d ,&in 's dn ,/) di,e"i ,'s 1 !+ P*' %e!#din di#i d*!
chamber (n& ,e"isi 10 !L !e'no* dn die*#si s!$i se!# $*' 'e",s)i+ P*'
%e!#din di%'i8si d*! o8en $d s#)# 1109 se*! 1 !eni'+
,+ Pen(i$n E*#en
Di,#' e*#en (n& 'e"di"i d"i %*o"o-o"!:se'on:!e'no* :13:13 88
se,n(% 10 !L+ Di$i$e' %*o"o-o"! se,n(% !L3 se'on se,n(% 13 !L dn
!e'no* se,n(% 13 !L %e d*! *,# #%#" 10 !L %e!#din di&o6o& )in&&
)o!o&en+ Chamber%e!#din di6en#)%n den&n e*#en (n& 'e*) di,#'+
+ Peno'o*n dn Pen&e*#sin
S#,-"%si (n& 'e*) di$e%'%n $e*"#'n(3 !sin&.!sin& di'i!,n&
se,n(% 10 !& dn di*"#'%n den&n 1 !L !e'no*+ Se*n6#'n( !sin&.
!sin& -"%si di'o'o*%n se,n(% L $d $*' KLT den&n 6"% 1 ! n'"
'o'o*n+ Ke!#din $*' die*#si den&n e*#en d*! chamber s!$i ,'s 's
$*'+
d+ Iden'i-i%si
P*' (n& 'e*) die*#si3 di!'i s$o'.s$o' (n& 'e",en'#% $d $*' di ,/)
*!$# U den&n $n6n& &e*o!,n& 7 n!+ S$o' !en&nd#n& -*8onoid
$,i* s$o' ,e"/"n %#nin&.o"n(e+ P*' 'e"se,#' %e!#din di$$"%n #$
!oni dn di*i)' %e!,*i di ,/) sin" U 7 n!+ S$o' !en&nd#n&
-*8onoid di'ndi den&n sedi%i' '# 'n$ $e"#,)n /"n+
+
+626 K#3%.3#9$ L;$ T$;$ P#";#.$9
+ Pen(i$n P*'
Disi$%n $*' KLT Si*i% Ge* GF24den&n #%#"n 1010 !3 %e!#din
$d ,&in 's dn ,/) di,e"i ,'s 1 !+ P*' %e!#din di#i d*!
chamber (n& ,e"isi 10 !L !e'no* dn die*#si s!$i se!# $*' 'e",s)i+ P*'
%e!#din di%'i8si d*! o8en $d s#)# 1109 se*! 1 !eni'+
12
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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,+ Pen(i$n E*#en
Di,#' e*#en (n& 'e"di"i d"i %*o"o-o"!:se'on:!e'no* :13:13 88
se,n(% 10 !L+ Di$i$e' %*o"o-o"! se,n(% !L3 se'on se,n(% 13 !L dn
!e'no* se,n(% 13 !L %e d*! *,# #%#" 10 !L %e!#din di&o6o& )in&&
)o!o&en+ Chamber%e!#din di6en#)%n den&n e*#en (n& 'e*) di,#'+
+ Peno'o*n dn Pen&e*#sin
S#,-"%si (n& 'e*) $osi'i- !en&nd#n& -*8onoid %e!#din di,#' $i'
$d $*' (n& 'e*) di%'i8si+ P*' %e!#din die*#si den&n e*#en d*!
chamber s!$i ,'s 's $*'+
d+ Iden'i-i%si
P*' (n& 'e*) die*#si3 di!'i s$o'.s$o' (n& 'e",en'#% $d $*' di ,/)
*!$# U den&n $n6n& &e*o!,n& 7 n!+ S$o' !en&nd#n& -*8onoid
$,i* s$o' ,e"/"n %#nin&.o"n(e+ S$o' (n& ,e"/"n %#nin&.o"n(e
%e!#din di%e"o% dn di*"#'%n den&n !L !e'no* dn dis"in&+
+626? U7$ K"%'#$ I3&. !" KLT D' D$%"$
+ Pen(i$n P*'
Disi$%n $*' KLT Si*i% Ge* GF24den&n #%#"n 1010 !3 %e!#din
$d ,&in 's3 ,/)3 %nn3 dn %i"i di,e"i ,'s 1 !+ P*' %e!#din di#i
d*! chamber (n& ,e"isi 10 !L !e'no* dn die*#si s!$i se!# $*'
'e",s)i+ P*' %e!#din di%'i8si d*! o8en $d s#)# 1109 se*! 1 !eni'+
,+ Pen(i$n E*#en
Di,#' e*#en (n& 'e"di"i d"i %*o"o-o"!:se'on:!e'no* :13:13 88
se,n(% 10 !L+ Di$i$e' %*o"o-o"! se,n(% !L3 se'on se,n(% 13 !L dn
!e'no* se,n(% 13 !L %e d*! *,# #%#" 10 !L %e!#din di&o6o& )in&&
)o!o&en+ Chamber%e!#din di6en#)%n den&n e*#en (n& 'e*) di,#'+
+ Peno'o*n dn Pen&e*#sin
Iso*' )si* KLT P"e$"'i- di'o'o*%n se,n(% L $d #6#n& $*' KLT+Ke!#din $*' die*#si den&n e*#en d*! chamber s!$i ,'s 's $*'+ P*'
di%e"in&%n3 %e!#din die*#si %e!,*i den&n e*#en (n& s! $d ") (n&
,e",ed $*' di$#'"
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
19/21
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
20/21
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8/10/2019 Proposal Mpba Kelompok 4 Flavonoid
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Sdi%#n3 A+3 A+ De/3 R+ A*'-3 M++ As!/i3 nd M+I+ U!"+ 2017+ P)('o)e!is'"(
nd !ediin* $"o$e"'ies o- P)*e"i !"o"$ S)e--+ Boe"*+ E'"'s+
Pharmacogn 8ev Vol 9 ($$5. P&e: 7.=0+
S'i)e"3 O+ 200=+ N'#"* P"od#' Iso*'ion+ N'+ P"od+ Re$3 o*+ 2+ P+1.4
S#!"di% dn J/i+ 2012+ E%s'"% Ai" D#n U,i J*" Un Me!$e",i%i P"o-i* Li$id
dn Menin&%'%n Kd" SOD D") Ti%#s n& Di,e"i M%nn Tin&&i
Ko*es'e"o*+/e!icina+o*+ 47+ No 2+ Mei 2012+
1