quantitative metabolism 1. quantitative metabolism
TRANSCRIPT
Quantitative Metabolism
Niether ADP/ATP nor NAD(H)/NAD+ is allowed to accumulate in the cell and so the ADP/ATP and NAD(H )/NAD+ “pool” are very tightly controlled within the cell.
“Currency of the Cell”Concentration of ATP in cell = 6 µmol / g Cell Concentration of ADP in cell = 2.5 µmol / g Cell Concentration of NAD(H) in cell = 1.5 µmol / g CellConcentration of NAD+ in cell = 2.5 µmol / g Cell
Rate of ATP Consumption = µ/YATP = 0.4/10.5 mol / g Cell/h= 38,000 µmol / g Cell / h
Rate of NAD(H) Consumption = QO2 * 2 = 4 mmol/ g Cell/h= 4,000 µmol / g Cell / h
If no ATP produced, the ATP within the cell would be used up in (6 µmol / g Cell) /(38,000 µmol / g Cell / h) = 0.56 secIf no NAD(H) produced, the NAD(H) within the cell would be used up in (1.5 µmol / g Cell) /(4,000 µmol / g Cell / h) = 1.35 sec
Maximum Rate of ATP Production (Full Respiration)= QS*4 = 59,200 µmol / g Cell / hMaximum Rate of NAD(H) Production(Full Respiration) = QS*12 = 177,600 µmol / g Cell / h
If no ATP used, the ADP within the cell would be used up in (2.5 µmol / g Cell) /(59,200 µmol / g Cell / h) = 0.15 secIf no NAD(H) used, the NAD+ within the cell would be used up in (2.5 µmol / g Cell) /(177,600 µmol / g Cell / h) = 0.05 sec
Conclusion
The cell VERY TIGHTLY controls the ATP/ADP levels and the NAD(H)/NAD+ levels to ensure that the pools of these intermediates keep within very fine tolerances. This is done by elaborate cellular controls which control the rate of formation and the rate of use of these intermediates – broadly by regulating energy substrate uptake (production) and cellular growth (use).
“Comparison” with the HKMA
Foreign Currency Reserves = 122.3 billion US$
Exports = 628,137 million $HK = $US 80,530 millionImports = 576,328 million $HK=$US 73,888 million
IF NO INCOME GENERATED (no exports), the cost of imports would drain the surplus in 122,300/78,888 = 1.65 years
Quantitative Metabolism
What determines whether a particular reaction is “capable” of generating ATP or NAD(H)???
Quantitative Metabolism
A B C D E F G H
G0A G0
B G0C G0
D G0E G0
F G0G
HFA HF
B HFC HF
D HFE HF
F HFG
HRA HR
B HRC HC
D HRE HR
F HRG
HCA HC
B HCC HC
D HCE HC
F HCG
Some measure of the amount of energy released is necessary
Quantitative Metabolism
A requisite for ATp formation is that the enrgy released from a reaction is sufficient to “drive” the formation of ATP from ADP
Questions:
1. Is this a sufficient requirement?
2. If there is sufficicent energy for 2 or nATP to be formed , will they be formed??
Quantitative MetabolismThe concept of
“Substrate Level Phosphorylation”
is important here
The formation of ATP at the molecular level within a certain reaction step requires a particular type of enzyme
Quantitative Metabolism
ATP will ONLY be formed if the appropriate enzyme is present (an enzyme capable of substrate level phosphorylation) and the number of ATP formed is (almost?) always 1
Excess enrgy release is usually lost as HEAT
Quantitative Metabolism
For NAD(H) production, the only requirement is for an oxidation reaction to occur ,releasing one or more H+
Questions:1. Is this a sufficient requirement?2. If there is sufficicent H+ released for 2 or
nNAD(H) to be formed , will they be formed??
Quantitative Metabolism
Yes, this condition is both a requisite and sufficient condition. Since the H+ exchange doe not occur via an enzyme similar to SLP, then more than one NAD(H) may be formed. The stoiciometry of this reaction is simply related to how many H+ are relased in the coupled reaction. NAD+ hydrogenases simply interact with the H+ released and each H+ released can inteact with a separate hydrogenase. This is unlike a SLP reaction, where both the reactant is bound to the enzyme in conjunction with the ADP form which the ATP is formed. Without an effective NAD+ hydrogenase, the pH of the immediate environment of the reaction would fall very rapidly
Many other electron acceptors may be used by microorganisms, including: Sulfate, Nitrate, Metal Ions etc .These all also use NADH2- and NAD+ as “linked” or “coupled” reactions.
For example:SO4
2- + 8H+ + 8e- = S2- + 4H20actually represents two reactions:
4NAD(H) + 4H+ = 4NAD+ + 8H+ + 8e-
SO42- + 8H+ + 8e- = S2- + 4H20
_______________________________________________
SO42- + 4NAD(H) + 4H+ = S2- + 4NAD+ +4H20
Nitrification and Denitrification
Nitrification is an aerobic process (requiring oxygen).
The overall reactions are the following:
Nitrification:
NH3 + 1.5O2 = HNO2 + H20HNO2 + 0.5O2 = HNO3
What actually happens in terms of H+ and e- is the following:
Nitrification:
NH3 + 2H2O = HNO2 + 6H+ + 6e-
HNO2 + H2O = HNO3 + 2H+ + 2e-
Denitrification:
2HNO3 + 10H+ + 10e- = N2 + 6H20
Hence Nitrification produces H+ and e- and Dentrification requires H+ and e-.
As usual, these H+ and e - come from the reaction:
NAD(H) + H+ = NAD+ + 2H+ + 2e-
The balanced reactions for nitrification and denitrification (in terms of NAD(H) and NAD+) then become:
Nitrification:
NH3 + 3NAD+ + 2H2O = HNO2 + 3NAD(H) + 3H+
HNO2 + NAD+ + H2O = HNO3 + NAD(H) + H+
Denitrification:
2HNO3 + 5NAD(H) + 5H+ = N2 + 6H2O + 5NAD+
In nitrification, oxygen is used to regenerate the NAD(H) formed:
NAD(H) + H+ = NAD+ + 2H+ + 2e-
0.5O2 + 2e- = O2-
O2- + 2H+ = H2O-----------------------------------------------------------NAD(H) + 0.5 O2 + H+ = NAD+ + H2O
In nitrification, there is a nett use of NAD(H) from the energy generating pathways (using CO2 as a carbon source) and this is provided by the nitrification reaction.
In denitrification, a carbon and energy source provides the NAD(H) required to drive the denitrification reaction.