quantum pesticide residue detection

Pesticide Detection with Quantum Methods

Jun Steed Huang, Ph.D advisorSouthern University of Science and Technology

Zhiqiang Richard Zuo,Ph.D candidateZhejiang University

Yan Xu,Ph.D candidateNanjing University

May 6 2017 Weekly Graduate Talk

Majority pesticide is either Organophosphorus or Organochlorine

Organophosphorus compounds are organic compounds containing phosphorus. They are used primarily in pest control as an alternative to chlorinated hydrocarbons that persist in the environment. These compounds are highly effective insecticides, though some are also lethal to humans at minuscule doses and include some of the most toxic substances ever created by man, including sarin and VX nerve agents.

An organochlorine or chlorinated hydrocarbon is an organic compound containing at least one covalently bonded atom of chlorine that has an effect on the chemical behavior of the molecule.

Organophosphorus or Organochlorine are harmful

Quantum dot excitation of neurotransmitter or quantum effect alkali metal amplif ication

We simulated the toxic reaction mechanism of animal brain nerve to organic phosphorus, and designed

organic phosphorus detector.

We simulated the toxic reaction mechanism of the plant apical nerve to the organic chlorine, and designed the

organic chlorine detector.

Microfluidic Chip for Pesticides Detection （农药检测）

Pesticide Orgnic P

Primarily, chlorpyrifos interfere with signaling from the neurotransmitter acetylcholine.

One chlorpyrifos metabolite, chlorpyrifos-oxon, binds permanently to the enzyme acetylcholinesterase,

preventing this enzyme from deactivating acetylcholine in the synapse.

By irreversibly inhibiting acetylcholinesterase, chlorpyrifos leads to a build-up of acetylcholine

between neurons and a stronger, longer-lasting signal to the next neuron.


Neurotransmitter


TMB 色显剂


System

chip

pump

meter

frame light

display

receiver

power

Microfluidic Chip for Pesticides Detection （农药检测）（ 1 ） CuFe2O4/GQDs nano-material colorimetric micro-flow control

MixNano

More amplification better detection limit.

Principle


Formulae

BoxidizedTMTMBOH

OHMNPs →+

→

→

22

22胆碱氧化酶

乙酰胆碱酯酶

胆碱胆碱乙酰胆碱

Org P

×

Color reaction

Inhibit the reaction

Peroxidase Nanoparticles

（ 1 ） Copper ferrite CuFe2O4/ GQDs (Graphene Quantum

Dots) Magnetic Nano-Particles (MNP)

Ach AchE


Trace

~1mm/sec

Microfluidic Chip for Pesticides Detection

A

0 20 40 60 80 100

-200

0

200

400

600

800

Intis

ity /

a.u.

2 Theta / degree

a

b

c

d

B Test1

Comparison of catalyt ic activity

CuFe2O4/GQDs Characterization and

performance

Observation

0.0 0.6 1.2 1.8 2.4 3.0

20

40

60

80

100

Rel

ativ

e A

ctiv

ity /

%

TMB / mM

C

15 25 35 45 55 65

20

40

60

80

100

Rel

ativ

e A

ctiv

ity /

%

Temperature / oC

A

1 3 5 7 9 11

20

40

60

80

100

Rel

ativ

e A

ctiv

ity /

%

pH

B

Param

eter optimization 图 2.8 基于 CuFe2O4/GQDs 比色法实验条件优化

Fig. 2.8 Conditions optimization of colorimetric assay based on CuFe2O4/GQDs

图 2.4 CuFe2O4/GQDs 合磁性米材料的复纳 TEM和 XRD 图Fig. 2.4 TEM andXRD image for the prepared CuFe2O4/GQDs magnetic

nanoparticles.

Test2

图 2.6 基于 CuFe2O4, GQDs 和 CuFe2O4/GQDs比色色反比显应对图Fig.2.6 Comparison of color reactions of as-prepared nonmaterials GQDs (a),

CuFe2O4 (b) and CuFe2O4/GQDs (c)by colorimetric assays

图 2.9 CuFe2O4/GQDs 的稳态动力学测试。D

y

n

a

m

i

c

s


More results

图 2.9 CuFe2O4/GQDs 的稳态动力学测试。Fig. 2.9 Steady-state kinetic assay of CuFe2O4/GQDs.

500 560 620 680 740 8000.000

0.125

0.250

0.375

0.500

Abs

/ a.

u.

Wavelength / nm

500 300 150 50 400 200 100 2

H2O

2(mM)A

0 120 240 360 480 6000.000

0.125

0.250

0.375

0.500

Abs

/ a.

u.C

H2O2 / mM

B


Mix Structure

Optimization

Inner r ib type Cycle merge type


More mix structures

Optimization +

Compound


A

B

Chips

Linearity

comparison

Interference

Star mixer


Data samples

Portable Organochlorine Detector with Wireless Connections

项目介绍

意课题义

系统设计硬件设计

化学设计件软设计

课题意义化学设计系统设计硬件设计结论宿迁学院

18

算法设计

结论

软件设计算法设计

19

课题意义化学设计系统设计硬件设计结论软件设计算法设计

Men who work with 2,4-D are at risk for abnormally shaped sperm and thus fertility problems

Research background and significance

In the international trade of agricultural products, the world put quality and safety issues in the first place. At present, China's agricultural product quality and safety are often less than the export of technical indicators. To solve the problem of quality and safety of agricultural products need to start from the agricultural production process and quality inspection system.

Relative to foreign countries, China's detection technology and testing standards are relatively backward. The system uses advanced optoelectronic rapid detection technology, in the detection speed and detection stability have a certain advantage

The project research, collection of photoelectric conversion technology, chemiluminescence technology, photoelectric rapid detection technology, two-dimensional code reading and writing technology, embedded technology and data fusion technology and other disciplines of the latest achievements, the completion of agricultural products from the production, transit and consumption. The whole process of monitoring the basket. As shown on the right:

物流企业

品加工企农产业

政府管部监门

消者费品生企农产产业

数据库

品零售商农产

品流产数据流

无基站线

种植、培育信

息

加工信息

物流信息

消费信息

查询

请求

查询

请求

返回查询结果

检测仪

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21


pota

ssiu

m p

erm

anga

nate

Administrator, 11/10/2016

Current Technologies:

1. Biometric technology: the use of biological physiological and biochemical reactions to determine pesticide residues and pollution. This biometric technique does not require pretreatment of the sample, but it is highly demanding on the test organism. The results can not determine the pesticide variety, the biological culture time is long, and there may be false positive or false negative.

2. Rapid detection method: the current application of the most rapid measurement method - enzymatic, according to the organic phosphorus and carbamate insecticide target catase - acetylcholinesterase (AChE) inhibited the degree of detection of the two types of killer pesticide residue. The method has the advantages of quick and easy method, simple pretreatment, no need for instrumentation, but only organic phosphorus and amino acid ester insecticides. The classification sensitivity is closely related to the enzyme, color reaction time and temperature.

3. Electrochemistry: the use of the measured substance in the solution in the chemical reaction, the detection of the potential or changes in the amount of quantitative and qualitative analysis of the method, belonging to physical and chemical analysis methods.

4. Photoelectric detection method: the use of pesticide chemiluminescence principle, through the photoelectric conversion technology, data acquisition and processing technology, data, fusion technology to achieve pesticide concentration - light intensity - the relationship between the voltage, and then measure the concentration of pesticides.

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Electrochemistry

Electrical <- > Chemistry

oxidize reduce

The project is developed into a portable agricultural product detector by means of photoelectric rapid detection technology, embedded technology and LabVIEW technology. The instrument has the advantages of small volume, fast detection speed and information collection, and can collect the name, time, place and agricultural product The information passed to the agricultural product monitoring center, and through two-dimensional code reading and writing function to write or scan the information found in the agricultural products to form a regional pesticide residue detection and early warning system.

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Quantum effect alkali metal amplifier

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UV

steed

The first choice of 2,4-dichlorophenoxyacetic acid (2,4-D dimethylamine, Herbicide herbicide) as a pesticide reagent and potassium permanganate reaction, due to the use of light conversion chemiluminescence method can be more. The conversion of a weak luminescent substance or a non-luminescent substance into a strong luminescent material can significantly improve the sensitivity of the photodetection. UV degradation of 2,4-dichlorophenoxyacetic acid generated by the polyphenols degradation products 2-chloro hydroquinone and 4-chloro catechol and potassium permanganate in H2SO4 medium reaction will have 690nm optical radiation. It is precisely because Mn (II) in the excited state to emit photoelectron into the ground state when the chemical spectrum is 690nm.

2 drops of liquor, beer or wine are used as catalyser to boost the reactions:

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第二次选择新农药杀虫剂 Insecticide （ C15H11BrClF3N2O&C22H17ClF3N3O7 ），

4- 溴 -2-(4- 氯苯基 )-1- 乙氧基甲基 -5- 三氟甲基吡咯 -3- 腈与 7- 氯 -2,5- 四氢 -2-[ 甲氧

基羰基（ 4- 三氟甲氧基苯基）氨基甲酰基 ] 茚并 [1,2-e][1,3,4-] 恶二嗪 -4a- 羧酸甲酯

与高锰酸钾进行反应，原理同理第一次实验。The second choice of new pesticide Insecticide (C15H11BrClF3N2O &

C22H17ClF3N3O7),

4-bromo-2- (4-chlorophenyl) -1-ethoxymethyl-5-trifluoromethylpyrrole- Chloro-2,5-tetrahydro-2- [methoxycarbonyl (4-trifluoromethoxyphenyl) carbamoyl] indeno [1,2-e] [1,3,4] -carboxylic acid methyl ester

with potassium permanganate reaction, the principle of the same reason the first experiment.

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第三次选择防霉剂 Fungicide N- （ 1-cyano-1,2-dimethylpropl ） - （ 2,4-

dichlorophenoxy ） proganamide 由日本与巴斯夫公司（原美国氟胺公司）在 1995 年

共同开发成功的内吸传导型稻瘟病防治药剂。

(2,4-dichlorophenoxy) proganamide was developed by Japan and BASF Corporation

(Former US Fluoramine Corporation) in 1995 to develop a successful combination of

Fungicide N- (1-cyano-1,2-dimethylpropl) Suction - type rice blast control agents.

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Trial design:

1, ARM + touch screen + hardware

equipment;

2, single-chip + hardware equipment;

3, FPGA + hardware equipment;

4, 4-channel DTMF controller +

hardware device.

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Design 1: ARM + touch screen + hardware equipment

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Figure 1 Schematic diagram of a chemical test


First generation product

Figure2 Touch screen

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Figure 4 the internal structure of quantum detector

Figure 3 touch screen display interface


Advantages: touch screen control, high integration, portable.

Disadvantages: A / D chip bit number is not enough, interfere with the signal more, affecting the test results.

Design 2: single-chip + hardware equipment

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The second generation

Advantages: wireless control, simple operation, the host computer control, to achieve the combination of the Internet +.

Disadvantages: low sampling rate, strong interference signal, stability is not strong

Design 3 、 4 channel DTMF controller + hardware

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The third generationAdvantages: strong control, strong operation

Disadvatages: Need to tune the level of the sound to avoid interference

Future Design:

FPGA+hardware

Advantages: can be cut, can be expanded, can be upgraded

Disadvantages: difficult

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System software design: The design of the fingerprint camera, the design of the authentication camera, the design of the examination system, the control design of the lower computer, and the protection of the green base and the rights and interests of the consumers by strengthening the management of the supervisors 。

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用户登录程序检测主程序程序


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The algorithm group applies a data fusion method suitable for repeated sampling of single sensor to the treatment of pesticide residue detection data. The data grouping is carried out by statistical theory, and the support probability is fuzzified. The determination of the support interval is discussed by section, So that the fusion data than the previous use of absolute support probability and support the results obtained more stable, and then to achieve the concentration - light intensity (voltage) between the relationship. Quantum Gaussian Distribution


Solved problem: (1) completed the design of a portable chemical test box, the

chemical reaction can be done without going to the chemical laboratory.

(2) completed the pesticide residue detection system of the initial debugging and packaging, the photoelectric quantum signal and noise signal can be divided.

(3) completed the "Internet +", "test certification" applications, to achieve the system in the detection of data at the same time. Data is able to be uploaded to the database in real time.

Reached the goal: (1) The detection speed is controlled within 10 minutes per

batch. (2) can be 1 * 10-8g / ml within a variety of pesticide.

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项目简介硬件设计课题任务分配

软件设计结论算法设计化学反应

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项目简介硬件设计课题任务分配

软件设计结论算法设计化学反应

[email protected]

Thanks

https://www.researchgate.net/publication/313853976_Portable_Pesticide_Detector_using_Quantum_Effect_Photon_Multipliers_and_Data_Fusion_Divisions_based_on_Chemiluminescence

https://www.researchgate.net/publication/314112437_A_Microfluidic_Colorimetric_Biosensor_for_Chlorpyrifos_Determination_based_on_Peroxidase-like_CuFe2O4GQDs_Magnetic_Nanoparticles

N P KO S Ca

quantum pesticide residue detection

Environment