Code No: D0308JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABADM.Tech. II Semester Examinations April 2011ADVANCED ANIMAL CELL SCIENCE AND TECHNOLOGY (BIOTECHNOLOGY)Time: 3 hours Max. Marks.60Answer any Five QuestionsAll questions carry equal marks- - -1. Explain in detail the various factors that influence the growth of cells in a media. [12]2. Explain the following in reference to immortalizationa) Oncogene b) Telomerase [12]3. Discuss the origin, characteristic and maintenance of cell lines. [12]4. Give an account on a choice of techniques used for authentication of cell lines. [12]5. Write short notes ona) Somatic cell genetics.b) RNA silencing. [12]6. Illustrate how somatic cell fusion is achieved and write about their use in therapeuticcloning. [12]7. Explain how to elucidate the mechanism(s) involved in the phenotypic effects caused by a mutant allele. [12]8. How do induce gene knockout in a mouse carrying targeted mutation. Explain the process with a suitable example.

1.(a) Animal tissue & cell culture

(b) Biotechnology

(c) Interrelationships between (a) and (b) and significance (IL)

2.Cell lines in Biomedical Research

(d) Primary cell lines,their advantages and disadvantages

(e) Production process.

3.Mammalian cell culture and production process

(a) Application of Biotechnology in production of mammalian cell cultures

(b)Production process.

(i) Primary cells as raw materials

(ii)Condition for cell culture:aseptic media,aeration,freezing and storage etc.

(iii) Primary culture,cell lines and cloning

(iv) Isolation of issues

(v) Enzymatic degradation

(vi) Mechanical disaggregation

(vii) Slide coverslip culture

(viii) Flask culture (6 L)

4. Separation of viable and non viable cells and cell counting (IL)

5. Laminer-flow cabinet & modification (IL)

6. Metabolism of animal tissue culture (2 L)

7.Characteristic and growth of animal cell cultures

(a) Batch-culture

(b) Continuous culture

(c) Fed-back culture

8.Bioreactiors for animal's cells, monitoring and control of bioreactors in animals cell cultures.

(a) Importance of bioreactors for animal cell culture

(b) Chemostats and turbiostats

(c) Gas-distribution bioreactors

(d) Stirred DNA technology of animal cell

9.Recombinant DNA technology of animal cell

(a) Overview of recombinant DNA and recombinant DNA technology

(i) Recombinant DNA and significance

(ii) Vectors,restriction enzymes,and clones

(iii) Gene splicing and synthesis of new proteins

(iv) Hybridoma; production & selection(4 L)

10.Genetic engineering of Animals cells

(b) Bulk production of proteins by genetically engineered animal cells

(c) Genetically engineered proteins in medicine, agriculture and industries(3 L)

11.Transgenic Animals

(a) Definition and significance

(b) Processing for developing transgenic animals.

12.Down stream processing and protein production recovery

(a) Definition and significance

(b) Processing steps

13.Blood serum fractionation and growth factors

(a) Overview of blood plasma, serum etc.

(b) Functions and origin of plasma proteins

(c) Fractionation of serum proteins

(d) Growth factors

(i) Definition and characteristic features

(ii) Nerve growth factor (NGF)

(iii) Epidermal growth factor (EGF)

(iv) Transgenic growth factory (TGF) BI,B2, B2

(v) Platelet-derived growth factors (PDGF)

(vi) Fibroblasts growth factors(PDGF)

(vii) Others peptides(9L)

Draw neat and labelled diagrams wherever necessary? ? ? ? ?

1. Explain in detail the three dimensional culture. Add a note on tissue engineering.

2. (a) What are different equipments used for animal cell culture?(b) Explain cell cultured based vaccines.(c) Write characters of cultured cells.

3. (a) Describe the role of CO2 in culture medium preparation.(b) Write briefly the measurement of cell death.(c) Describe disaggregation of tissue and primary culture.

4. Explain the biology and characterization of culture cells. Add a note on their applications.

5. (a) Write briefly on patterns of growth measurement.(b) Describe the embryonic stem cells.(c) Write briefly on somatic cell genetics.

6. (a) Write about maintenance of cell culture.(b) Describe briefly the structure of animal cell.(c) Explain cell synchronization.

7. Describe various techniques involved in invitro mammalian cell culture.

8. (a) Explain the chemical functions of constituents of culture medium.

(b) Describe simple growth medium.(c) Describe briefly the steps involved in cell cloning.

1. Write an essay on chemical and physical functions of different constituents of culture medium.2. (a) Explain primary cell line culture.(b) Describe cell cultured based vaccines.(c) Describe the application of serum and protein free defined media in culture medium preparation.

3. (a) Explain briefly Apoptosis.(b) Write about scaling-up of animal cell culture.(c) Write briefly about measurement of cell death.

4. What are different steps involved in cell cloning? Add a note on micromanipulation.

5. (a) Write briefly on measurement of viability.(b) Explain cell separation.(c) Give an account of the applications of embryonic stem cells.

6. (a) Describe the patterns of growth measurement.(b) Explain briefly somatic cell genetics.(c) Describe organ culture.

7. Describe in detail the three dimensional culture. Add a note on tissue engineering.

8. (a) Explain role of supplements in culture medium preparation.(b) Write about cell transformation.(c) Describe briefly the structural organization of animal cell. 

1. What are different steps involved in cell cloning? Add a note on micromanipulation.

2. (a) Describe simple growth medium.(b) Explain role of CO2 in culture medium preparation.(c) Write briefly on histolytic culture.

3. (a) Explain briefly the applications of embryonic stemcells.(b) Describe disaggregation of tissue and primary culture.(c) Write briefly about cell transformation.

4. Describe the detail of three dimensional culture. Add a note on tissue engineering.

5. (a) What are different types of equipments & materials used in animal cell culture techniques?(b) Explain role of supplements in culture medium preparation.

6. (a) Describe briefly stem cell culture.(b) Write about patterns of growth measurements.(c) Explain cell-cultured based vaccines.

7. Write an essay on Apoptosis.

8. (a) Describe cell synchronization.(b) Describe the Biology of cultured cells.(c) Explain briefly the metabolic functions of different constituents of culture medium. 

All questions carry equal marksDraw neat and labelled diagrams wherever necessary? 

1. Describe in detail the ultra structure of animal cell.

2. (a) Explain the role of CO2 in culture medium preparation.(b) Write briefly about cell transformation.(c) Write about patterns of growth measurements.

3. (a) Describe simple growth medium.(b) Write briefly about cell separation.(c) What are the characters of cultured cells?

4. Explain various techniques involved in invitro mammalian cell culture.5. (a) Write briefly about measurement of cell death.(b) Explain cell cultured based vaccines.

6. (a) Describe briefly Somatic cell genetics.(b) Explain briefly on organ culture.(c) Write about primary cell line culture.

7. Describe in detail the three dimensional culture.Add a note on tissue engineering.

8. (a) Explain the role of serum in culture medium preparation.(b) Write about measurement of viability.(c) Write briefly about sealing-up of animal cell culture.

1) List the basic infrastructure required to start an animal cell culture laboratory. What arethe precautions and aseptic conditions to be maintained while working in the animal cellculture lab and why?2) Write the physicochemical properties of a culture medium. What are the advantages anddisadvantages of adding serum in the animal culture medium?3) Give an account of different techniques used for the authentication of cell lines.4) What are the methods used for isolation of single animal cells? Differentiate primaryculture and established culture.5) Define transfection? Describe the various techniques utilized for introducing foreignDNA into animal cells. Explain the therapeutic and diagnostic application of recombinantproteins.6) Give a detailed account of therapeutic cloning? What are the applications of therapeuticcloning?7) What are caspases? What are the different types of caspases? How caspases are involvedin cell death pathway. Add a note on roles of BCl2 family in apoptotic events.8) What is tissue engineering? How is it plays a significant role in regenerative medicine.

Code No: 2422301IV B.Tech II Semester Regular Examinations, April/May 2009ANIMAL CELL SCIENCE & TECHNOLOGY

set-2(Bio Technology )Time: 3 HoursMax. Marks 80Answer any FIVE questionsAll questions carry equal marks********1) Describe aspects of biology relating to cellular organelles. Explain how cell organizationdepends on dynamic network of cytoskeleton.2) What is protein free defined media? How is it different from serum free media?

Discussbriefly the main components of protein free defined media. Write the advantages andlimitations of protein free defined media.3) Give an account of different assay for measuring cell viability and cytotoxicity.4) What are the characteristic features of primary cell culture? What are the steps involvedin the generation of primary cell culture.5) Define cell synchronization? What are the different techniques available forsynchronizing the cells at a particular growth phase? Discuss briefly metabolic blockadesused for cell synchronization.6) Define somatic cell fusion. How are they used in the process of reproductive cloning?Give an account of therapeutic application of somatic cell nucleus transfer.7) What is an organ culture? What are the types of organ cultures? Discuss briefly variousfactors that greatly influence the viable state of an organ culture.8) Give an account of current status and future prospective of tissue engineeringtechnologies. Give an account of unique goals of tissue engineering technology.*********

Code No: 2422301IV B.Tech II Semester Regular Examinations, April/May 2009ANIMAL CELL SCIENCE & TECHNOLOGY

set-3(Bio Technology )Time: 3 HoursMax. Marks 80Answer any FIVE questionsAll questions carry equal marks********1) What is biosafety cabinet? Discuss in detail the different types of BSC available forcell culture purpose and their suitability to a particular cell culture technique?2) Discuss briefly the important constituents of a typical culture medium and theirsignificance in the context of cell growth in culture medium.3) Give an account of factors that favor the spreading, migration and proliferation ofcells in culture. Discuss about the cell adhesion molecules that allow the cells toadhere to surface and form a monolayer culture.4) Describe the technique involved in the subculturing/passaging of monolayer culture.What is the passaging protocol for suspension culture? How are dead cells removedfrom suspension culture? Describe the important criteria that should be taken intoaccount for subculture.5) What are the products of animal cell culture? Write the applications of importantproducts from animal cell culture technology.6) Discuss briefly about stem cell plasticity. Give an account of medical

implication ofstem cell plasticity.7) Discuss different stress signals responsible for triggering apoptosis.8) Stem cell based therapy and tissue engineering – explain how they are interlinked

Code No: 2422301IV B.Tech II Semester Regular Examinations, April/May 2009ANIMAL CELL SCIENCE & TECHNOLOGY(Bio Technology )

set-4

Time: 3 HoursMax. Marks 80Answer any FIVE questionsAll questions carry equal marks********1) What are the important characteristics of monolayer and suspension culture? Giveexamples of cells that can be grown as monolayer culture and as suspension culture.2) Discuss the physical, chemical and metabolic functions of major constituents of a culturemedium.3) Describe in details the different assays commonly used in the cytotoxicity testing ofdrugs and chemicals.4) Write the principles of FACS. Discuss the techniques involved in the separation of cellsusing FACS.5) Discuss any two techniques used exclusively for the scaling up of a monolayer culture.6) What are the sources of pluripotent embryonic stem cells? What are the properties ofpluripotent stem cells? How are stem cells isolated from those sources?7) Explain the biochemical changes occurred in the cells during apoptosis. Discuss theintrinsic pathway of apoptosis with illustrations.8) Discuss briefly about the various aspecthttp://www.blogger.com/post-edit.g?blogID=5896984323109998991&postID=2353572191224520916s of engineering design in the creation of artificialtissues. Give an account of potential economic benefits that could be derived from tissueengineering technologies.