QUIZ Lab 2QUIZ Lab 2

Ag-Ab Reaction Ag-Ab Reaction

10 points, 10 minutes10 points, 10 minutes

Assist.Prof.Suwin Wongwajana,M.D.,M.Sc.Assist.Prof.Suwin Wongwajana,M.D.,M.Sc.

Dept.of Microbiology, Fac of Medicine, KKU.Dept.of Microbiology, Fac of Medicine, KKU.

Write yourWrite yournamename,,student codestudent code,,lab lab

gr.gr.DateDate

on the top of the paperon the top of the paper

8 items8 items , , 10 marks, 10 marks, 10 min.10 min.

1.1. Patient serum was diluted inPatient serum was diluted in serial 4-serial 4-folded manner. After adding the Agfolded manner. After adding the Ag and let the and let the reaction occurred. The reaction occurred. The precipitate was found until tube No.3. precipitate was found until tube No.3. TheThe antibody titer=antibody titer= ??(If(If dilution in tube No.1 = 1:4 ) dilution in tube No.1 = 1:4 )

a. 1:4

b. 1:16

c. 1:24

d. 1:64

e. 1: 256

2.2. About single radial immunodiffusion About single radial immunodiffusion (SRID), (SRID), Which sentence is correct?Which sentence is correct?

a. It is an agglutination technique.

b. It uses electricity in the test.

c. It is sensitive than ELISA.

d. It is a quantitative technique.

e. All of the above.

3.3. The steps inThe steps in immunoelectrophoresis immunoelectrophoresis (IEP) test included: (IEP) test included: a. let Ag react with Aba. let Ag react with Abbb. . puncture the gel, add Ag into the wellpuncture the gel, add Ag into the wellc. run the electrophoresis c. run the electrophoresis d. add Ab into the troughd. add Ab into the trough

Which sequence is correct?

a. abcd b. bcda c. cdba

d. dabc e. No correct answer

4.4. The results of The results of immunodiffusion was immunodiffusion was shown, shown, Which sentence is correct?Which sentence is correct?

a. Ag1and Ag2 are non-identity

b. Ag1and Ag2 are partial identity

c. Ag1and Ag3 are partial identity

d. Ag2 and Ag3 are identity

e. No correct answer

Ab Ab

Ag3Ag2Ag1

5.5. About blood grouping test, If you About blood grouping test, If you have blood gr.Ohave blood gr.O, , Which sentence is Which sentence is correct?correct?

a. RBC have Ag A and Ag B.

b. In serum have anti-A, No anti-B

c. In serum have anti-B, No anti-A.

d. RBC have no Ag A and no Ag B.

e. No correct answer.

6.6. AboutAbout ELISA technique, ELISA technique, Which sentence is incorrect?Which sentence is incorrect?

a. Use enzyme as the labelling agent.b. Can detect both Ag or Ab.c. Must add the substrate then observing

the color changes.d. The intensity of the color changed is

direct correlated with the concentration of the unknown.

e. Fluorescent microscope was required in order to assess the result.

7.7. Which sentence is incorrect?Which sentence is incorrect?

a.Gr O can give the RBC to the Gr.B

b.Gr O can give the RBC to the Gr.AB

c.Gr A can give the RBC to the Gr.AB

d.The lowest percentage of Thai Bl.Gr.is AB

e.The highest percentage of Thai Bl.Gr.is B

1ml

99

4.5

0. 5

ml

9.9

0. 1

ml

1:41:80

3

1ml

Dilution

Final Dilution

.............

...

.............. ......

..........

.............

... .............

...

..............

8.8.

AnswerAnswerNo.No. -17 -17, , 1 mark 1 mark eacheach No.No. 8, 0.5 8, 0.5mark eachmark each

Total Total 10 10marksmarks

= 4 X 4 X 4= 4 X 4 X 4

1.1. Patient serum was diluted inPatient serum was diluted in serial 4-serial 4-folded manner. After adding the Agfolded manner. After adding the Ag and let the and let the reaction occurred. The reaction occurred. The precipitate was found until tube No.3. precipitate was found until tube No.3. TheThe antibody titer=antibody titer= ??(If(If dilution in tube No.1 = 1:4 ) dilution in tube No.1 = 1:4 )

a. 1:4

b. 1:16

c. 1:24

d. 1:64

e. 1: 256

a. It is an agglutination technique.

b. It uses electricity in the test.

c. It is sensitive than ELISA.

d. It is a quantitative technique.

e. All of the above.

2.2. About single radial immunodiffusion About single radial immunodiffusion (SRID), (SRID), Which sentence is correct?Which sentence is correct?

3.3. The steps inThe steps in immunoelectrophoresis immunoelectrophoresis (IEP) test included: (IEP) test included: a. let Ag react with Aba. let Ag react with Abbb. . puncture the gel, add Ag into the wellpuncture the gel, add Ag into the wellc. run the electrophoresis c. run the electrophoresis d. add Ab into the troughd. add Ab into the trough

Which sequence is correct?

a. abcd b. bcda c. cdba

d. dabc e. No correct answer

Ab Ab

Ag3Ag2Ag1

4.4. The results of The results of immunodiffusion was immunodiffusion was shown, shown, Which sentence is correct?Which sentence is correct?

a. Ag1and Ag2 are non-identity

b. Ag1and Ag2 are partial identity

c. Ag1and Ag3 are partial identity

d. Ag2 and Ag3 are identity

e. No correct answer

5.5. About blood grouping test, If you About blood grouping test, If you have blood gr.Ohave blood gr.O, , Which sentence is Which sentence is correct?correct?

a. RBC have Ag A and Ag B.

b. In serum have anti-A, No anti-B

c. In serum have anti-B, No anti-A.

d. RBC have no Ag A and no Ag B.

e. No correct answer.

6.6. AboutAbout ELISA technique, ELISA technique, Which sentence is incorrect?Which sentence is incorrect?

a. Use enzyme as the labelling agent.b. Can detect both Ag or Ab.c. Must add the substrate then observing

the color changes.d. The intensity of the color changed is

direct correlated with the concentration of the unknown.

e. Fluorescent microscope was required in order to assess the result.

= O= O

7.7. Which sentence is incorrect?Which sentence is incorrect?

a.Gr O can give the RBC to the Gr.B

b.Gr O can give the RBC to the Gr.AB

c.Gr A can give the RBC to the Gr.AB

d.The lowest percentage of Thai Bl.Gr.is AB

e.The highest percentage of Thai Bl.Gr.is B

1ml

99

1:1001:8,000

4.5

1:101:80

,000

0. 5

ml

9.9

0. 1

ml

1:41:80

1:1001:8,0

00,000

1ml

3

Dilution

Final Dilution

8.8.

Write the-Write the-name of the name of the checkerchecker

--Total scoreTotal scoreSend them back to teacherSend them back to teacher

No.No. -17 -17, , 1 mark 1 mark eacheach No.No. 8, 0.5 8, 0.5mark eachmark each

Total Total 10 10marksmarks

•Good LuckGood Luck•Good LuckGood Luck