rainwater monitoring of rust spores
DESCRIPTION
Rainwater monitoring of rust spores. Charlie Barnes USDA-ARS Cereal Disease Laboratory. Rainwater monitoring of rust spores. Van Bowersox Karen Harlin Illinois State Water Survey NADP/NTN. Les J. Szabo Jerry L. Johnson Kim P. Nguyen USDA ARS CDL University of Minnesota. - PowerPoint PPT PresentationTRANSCRIPT
Rainwater monitoring of rust spores
Rainwater monitoring of rust spores
Charlie Barnes
USDA-ARS
Cereal Disease Laboratory
Charlie Barnes
USDA-ARS
Cereal Disease Laboratory
Rainwater monitoring of rust spores
Rainwater monitoring of rust spores
Les J. Szabo
Jerry L. Johnson
Kim P. NguyenUSDA ARS CDL
University of Minnesota
Les J. Szabo
Jerry L. Johnson
Kim P. NguyenUSDA ARS CDL
University of Minnesota
Funding: USDA- ARS, USB and MNSR&PC
Van Bowersox
Karen HarlinIllinois State Water Survey
NADP/NTN
Van Bowersox
Karen HarlinIllinois State Water Survey
NADP/NTN
Student workersStudent workers
Andrea Carlson
Faith Durham
Kate Butler
Andrea Carlson
Faith Durham
Kate Butler
John Bulter
Jackie Koch
Josh Rosnow
Yibai Li
John Bulter
Jackie Koch
Josh Rosnow
Yibai Li
OutlineOutline
The real-time PCR assay Project one: Puccinia graminis spores in
rain Project two: Phakopsora pachyrhizi
spores in rain
The real-time PCR assay Project one: Puccinia graminis spores in
rain Project two: Phakopsora pachyrhizi
spores in rain
Fungal Fungal
18S ITS1 5.8S ITS2
P. graminis nested assayP. graminis nested assay
Rust RustProbe
18S ITS1 5.8S
P. graminis nested assayP. graminis nested assay
Verify amplicon size Sequence select positives
Verify amplicon size Sequence select positives
Project OnePuccinia graminis in rain
Project OnePuccinia graminis in rain
NADP sites used to monitor P. graminis in rain in 2004NADP sites used to monitor P. graminis in rain in 2004
A typical NADP stationA typical NADP station
P. graminis project outlineP. graminis project outline
Rain is collected weekly at NADP sites Rain is sent to Illinois and filtered Filters are dried and sent overnight Remove debris from filter (day 1) DNA is extracted (day 2) Run real-time PCR assay (day 3)
Rain is collected weekly at NADP sites Rain is sent to Illinois and filtered Filters are dried and sent overnight Remove debris from filter (day 1) DNA is extracted (day 2) Run real-time PCR assay (day 3)
P. graminis in rain-May 18P. graminis in rain-May 18
Mod. sporeload
SR fieldreports
P. graminis in rain-May 25P. graminis in rain-May 25
Mod sporeload
SR fieldreports May 18
P. graminis in rain-June 1P. graminis in rain-June 1
Mod sporeload
SR fieldreports
P. graminis in rain-June 8P. graminis in rain-June 8
Mod sporeload
SR fieldreports
3 weeks later
P. graminis in rain-June 15P. graminis in rain-June 15
Mod sporeload
SR fieldreports
3 weeks later
P. graminis in rain-June 22P. graminis in rain-June 22
Mod sporeload
SR fieldreports
3 weeks later
P. graminis in rain-July 20P. graminis in rain-July 20
Mod sporeload
SR fieldreports
SummarySummary
Detection limit of 1-10 spores Detect stem rust spores 3 weeks prior
to 1st reported infections consistently along the Puccinia pathway
Regional trends confirms assay What one would expect from the biology of
the organism
Detection limit of 1-10 spores Detect stem rust spores 3 weeks prior
to 1st reported infections consistently along the Puccinia pathway
Regional trends confirms assay What one would expect from the biology of
the organism
Project TwoPhakopsora pachyrhizi in rain
Project TwoPhakopsora pachyrhizi in rain
Data for 2005 and 2006Data for 2005 and 2006
Fungal Php specific*
18S ITS1 5.8S ITS2
Ph. pachyrhizi nested assayPh. pachyrhizi nested assay
Rust RustProbe
18S ITS1 5.8S
*Frederick et al. 2002
US soybean production 2005by countyUS soybean production 2005by county
NADP sites used to monitor Ph. pachyrhizi in rainNADP sites used to monitor Ph. pachyrhizi in rain
Weekly trends: 2005-2006Weekly trends: 2005-2006P
erce
nt P
ositi
ve
May June July Sept.Aug.
2005
2006
Oct.
Comparison of 2005 & 2006Geographical Distribution (May-Aug)
Comparison of 2005 & 2006Geographical Distribution (May-Aug)
2005 2006
2005 2006
Comparison of 2005 & 2006Geographical Distribution (May-Aug)
Comparison of 2005 & 2006Geographical Distribution (May-Aug)
2005 2006
Comparison of 2005 & 2006Geographical Distribution (May-Aug)
Comparison of 2005 & 2006Geographical Distribution (May-Aug)
2005 2006
Comparison of 2005 & 2006Geographical Distribution (May-Aug)
Comparison of 2005 & 2006Geographical Distribution (May-Aug)
ASR field reports 2005ASR field reports 2005
ASR field reports 2006ASR field reports 2006
2005boundry
SummarySummary
Ph. pachyrhizi spores were detected throughout the soybean growing region of the U.S.
Frequency and spore load increased in 2006. Detection of spores in rain further north in
2006 matches increase in field reports. Rain data is consistent with ASR becoming
established in Southern U.S. and the Gulf Coast region of Mexico and Central America.
Ph. pachyrhizi spores were detected throughout the soybean growing region of the U.S.
Frequency and spore load increased in 2006. Detection of spores in rain further north in
2006 matches increase in field reports. Rain data is consistent with ASR becoming
established in Southern U.S. and the Gulf Coast region of Mexico and Central America.
Overall summaryOverall summary
So far, detect 2 rust pathogens in rain Adaptable assay system Currently have probes for:
P. coronata (7 different groups) P. recondita f.sp secalis P. striiformis P. triticina And others
So far, detect 2 rust pathogens in rain Adaptable assay system Currently have probes for:
P. coronata (7 different groups) P. recondita f.sp secalis P. striiformis P. triticina And others