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Rayat Shikshan Sanstha’s
Karmaveer Bhaurao Patil College Vashi
Autonomous College
Syllabus for Approval
Sr.
Heading Particulars No.
1 Title of Course F.Y.B.Sc. Microbiology
2 Eligibility for Admission 12th Science and equivalent
[of recognized Boards]
3 Passing Marks 40%
4 Ordinances/Regulations
(if any) -
5 No. of Years/Semesters One year/Two semester
6 Level U.G.
7 Pattern Semester
8 Status Revised
9 To be implemented from
2018-2019 Academic year
AC- / / 2018
Item No-
Rayat Shikshan Sanstha’s
KARMAVEER BHAURAO PATIL COLLEGE VASHI
(AUTONOMOUS COLLEGE) Sector-15- A, Vashi, Navi Mumbai - 400 703
Syllabus for F.Y.B.Sc. Microbiology
Program: B.Sc. Microbiology
Course: F.Y.B.Sc. Microbiology
(Choice Based Credit, Grading and Semester System with effect from the academic year 2018‐2019)
Preamble
Bachelor of Science (B.Sc.) in Microbiology is an under graduation programme of Department
of Microbiology, Karmaveer Bhaurao Patil College Vashi, Navi Mumbai [Autonomous College] Microbiology is a branch of science that studies microorganisms such as bacteria, protozoa,
algae, fungi, bacteria, viruses, etc. These studies integrate cytology, physiology, ecology,
genetics and molecular biology, evolution, taxonomy and systematics with a focus on
microorganisms.
The relevance and applications of microorganisms to the surrounding environment including
human life and Mother Nature becomes part of this branch. Since inception of this branch of
science, Microbiology has remained a field of active research and ever expanding in all possible
directions; broadly categorized as pure and applied science.
Different branches of Pure Microbiology based on taxonomy are Bacteriology, Mycology,
Protozoology and Parasitology, Phycology and Virology; with considerable overlap between
these specific branches over each other and also with other disciplines of life sciences, like
Biochemistry, Botany, Zoology, Cell Biology, Biotechnology, Nanotechnology, Bioinformatics,
etc. Areas in the applied Microbial Sciences can be identified as: Medical, Pharmaceutical, Industrial
(Fermentation, Pollution Control), Air, Water, Food and Dairy, Agriculture (Plant Pathology and
Soil Microbiology), Veterinary, Environmental (Ecology, Geomicrobiology); and the
technological aspects of these areas.
Microbiological tools have been extensively used to study different life processes and are cutting
edge technologies. There is a continual demand for microbiologists in the work force – education, industry and research. Career opportunities for the graduate students are available in
manufacturing industry and research institutes at technical level.
The content of a syllabus should be such that it maintains continuity with the course content of
higher secondary class and post graduate course. The present curriculum is made keeping this in
mind and is an effort to impart fundamental knowledge of the subject needed at this level. The
curriculum is designed as per the guidelines for Choice Based Credit System and reflects the
total credit, teaching hours and evaluation pattern.
Objectives of the Course:
• To enrich students’ knowledge and train them in the pure microbial sciences
• To introduce the concepts of application and research in Microbiology
• To inculcate sense of scientific responsibilities and social and environment awareness
• To help students’ build-up a progressive and successful career
Course Learning Outcome:
By the end of the course, a student should develop the ability to
1. Explain, coherently and effectively various genres of microbiology.
2. Discuss and compare prokaryotic and eukaryotic morphology.
3. Develop basic skills in practical microbiology.
4. Investigate nutritional needs of microorganisms.
5. Use different methods of control of microorganisms.
6. Examine various biomolecules.
7. Elaborate on diversity of microbial ecosystems.
8. Restate basic concepts of immunity.
9. Illustrate scope for industrial microbiology and basics of fermentation.
Scheme of examination for Each Semester: Continuous Internal Evaluation: 40 Marks (Common Test-20 Marks & 20 Marks for-
Assignment, Projects, Group discussion, Open book test, online test etc.) based on Unit III of
each paper.
Semester End Examination: 60 Marks will be as follows -
Theory: The Semester End Examination for theory course work will be conducted as
per the following scheme.
Each theory paper shall be of two hours duration.
All questions are compulsory and will have internal options.
I.
Q – I Subject questions from Unit – I (having internal options.) 20 M
Q – II Subjective questions from Unit – II (having internal options.) 20 M
Q – III Objective type questions based on both the Units with equal weightage.
20 M
Practical
The Semester End Examination for practical course work will be
II. conducted as per the following scheme.
Sr.
Marks% No. Particulars of Semester End Practical Examination
1 Laboratory Work 80
2 Journal 10
3 Viva 10
TOTAL
100
Scheme of examination for Each Semester: Continuous Internal Evaluation: 40 Marks (Common Test-20 Marks & 20 Marks for-
Assignment, Projects, Group discussion, Open book test, online test etc.) based on Unit III of
each paper.
Semester End Examination: 60 Marks will be as follows -
Theory: The Semester End Examination for theory course work will be conducted as
per the following scheme.
Each theory paper shall be of two hours duration.
All questions are compulsory and will have internal options.
I.
Q – I Subject questions from Unit – I (having internal options.) 20 M
Q – II Subjective questions from Unit – II (having internal options.) 20 M
Q – III Objective type questions based on both the Units with equal weightage.
20 M
Practical
The Semester End Examination for practical course work will be
II. conducted as per the following scheme.
Sr.
Marks% No. Particulars of Semester End Practical Examination
1 Laboratory Work 80
2 Journal 10
3 Viva 10
TOTAL
100
F. Y. B. Sc. Microbiology
SEMESTER I
Paper I [UG MB-101]: Fundamentals of Microbiology
Paper II [UG MB-102]: Basic Techniques and Applied Microbiology
Course Code Unit Topics Credits L /
Week
I Introduction to Microbiology & Prokaryotic 1
UG MB-101 Cell Structure
II Eukaryotic Cell Structure & function 2
1
III Microbial Evolution &Diversity 1
I Nutrition and Growth of Microorganisms 1
and Impact of Microorganisms
UG MB-102 II Control of microorganisms
2 1
III Basic Instrumentation 1
MBP-1 Practicals Basic Techniques of 2 6
Microbiology
SEMESTER II
I Basics of Biochemistry 1
UG MB-201
2
II Basic Virology 1
III Microbial ecosystem 1
I Introduction to Industrial Microbiology 1
UG MB-202
2
II Basic Immunology 1
III Infections 1
MBP-2 Practicals Methods in Microbiology 2 6
F.Y.B.Sc. Microbiology
Credit Based Semester & Grading System
2018-19
SEMESTER I Paper I
Course
Code
Title Credits
UGMB-
101
Fundamentals of Microbiology
2 Credits
(45 lectures)
Unit-I Introduction to Microbiology & Prokaryotic Cell Structure
1.1 Introduction to Microbiology
1.2 Microbes in our lives
1.3 History of Microbiology
1.4 Types of Microorganisms (Brief)
a. Bacteria
b. Archea
c. Fungi
d. Protozoa
e. Algae
f. Viruses
1.5 Microbiology in India
1.6 Classification of organisms & Basic Concepts of
Taxonomy
a. Five Kingdom System of Classification
b. Eight Kingdom System of Classification
c. Three Domain System of Classification
1.7 Prokaryotic Cell Structure & function
a. Cell wall
b. Cell membrane
c. Components external to cell wall-
capsule, slime layer, s layer, flagella, pili, fimbriae
d. Cytoplasmic matrix-Inclusion bodies, magnetosomes,
ribosomes, gas vesicles
e. Bacterial endospores
15 Lectures
Unit-II 2.1 Eukaryotic Cell Structure & function
a. Overview of Eukaryotic cell structure
b. The plasma membrane and membrane structure
c. Cytoplasmic matrix, microfilaments, intermediate
filaments, and microtubules
d. Organelles of the Biosynthetic-secretory and endocytic
pathways –endoplasmic reticulum &golgi apparatus.
Definitions of Lysosome, Endocytosis, Phagocytosis,
Autophagy, Proteasome
e. Eukaryotic ribosomes
f. Mitochondria
g. Chloroplasts
h. Nucleus –nuclear structure
i. External Cell organelles: cilia and flagella
j. Comparison of Prokaryotic and Eukaryotic Cells
15 Lectures
Unit-III Microbial Evolution &Diversity
3.1 Evolution of Microorganisms & Current Scenario
3.2 Diversity of Microbial World – I
a. Ricketssiae
b. Coxiella
c. Chlamydia
d. Mycoplasma
e. Actinomycetes
f. Archae
3.3 Diversity of Microbial World – II
a. Fungi
b. Algae
c. Protozoa
15 Lectures
F.Y.B.Sc. Microbiology
Credit Based Semester & Grading System
2018-19
SEMESTER I Paper II
Course
Code
Title Credits
UGMB-
102
Techniques & Applied Microbiology 2 Credits
(45 lectures)
Unit-I 1.1 Nutrition and Growth of Microorganisms
a. Nutrition: Macronutrients & Micronutrients including
growth factors
b. Table for general physiological functions
c. Types of Microorganisms based on nutritional
requirements
d. Uptake of nutrients: Passive diffusion, Facilitated
diffusion, Active transport, Group translocation (only
definitions)
e. Cultivation & Detection of Microorganisms
f. Culture media: Different types- Synthetic, Complex,
Anaerobic, Selective, Differential & Enriched medium
g. Growth: Definition, Growth Curve, Continuous Culture,
Measuring Microbial Growth, Quantitative Measurements:
Direct &Indirect Methods
h. Effects of solutes, osmotic tolerance, Temperature, Oxygen
1.2 Impact of Microorganisms
Impact of Microorganisms in various fields
a. Microbes in Food & Dairy Industries
b. Microbes in Production of Industrial Products
c. Microbes in Genetic Engineering & Biotechnology
d. Microbes in Environmental Microbiology
e. Microbes & Medical Microbiology
f. Microbes in Agriculture
g. Microbes in Bio-terrorism
h. Bioinformatics
i. Bioprocess Technology
15 Lectures
Unit-II 2.1 Control of microorganisms a. Definitions of frequently used terms
b. Physical
i. Dry and Moist heat- mechanisms, instruments used
and their operations
ii. Electromagnetic radiations-Ionizing radiations,
mechanism- advantages and disadvantages
iii. Bacterial proof filters
iv. Low temperature
v. Osmotic pressure
vi. Desiccation
15 Lectures
c. Chemical methods
i. Phenolics
ii. Alcohols
iii. Heavy metals and their compounds
iv. Halogens
v. Quaternary ammonium compounds
vi. Dyes
vii. Surface active agents/Detergents
viii. Aldehydes
ix. Peroxygens
x. Sterilizing gases
d. Biological Methods
e. Conditions influencing the effectiveness of antimicrobial
agents
f. Mechanisms of cell injury
2.2 Biosafety cabinets
Unit-III Basic Instrumentation
3.1 Microscopy
a. Compound Microscope and types
b. Electron microscope
c. New generations of Microscopes-Atomic Force microscopy
and SEM Include names
d. Care & use of Microscope
3.2 pH meter
3.3 Colorimeter
3.4 Centrifuge and types
3.5 Shaker and types
15 Lectures
F.Y.B.Sc. Microbiology Practicals
Credit Based Semester & Grading System
2018-19
Course
code
Title Credits
UGMB
P1
Section I- Basic Techniques and Applied Microbiology 1 Credit
(45 Lectures)
1. Orientation to Microbiology laboratory
2. Introduction to safety measures in laboratory and disposal of
microbial cultures
3. Microscopy- Study of Compound Microscope and its parts
Care and Maintenance of microscope
4. Study of Microscopic Morphology of Microorganisms:
a. Monochrome staining
b. Gram Staining
c. Hanging drop method (motility)
5. Special staining:
a. Capsule Staining
b. Cell Wall Staining
c. Endospore Staining
d. Metachromatic granules Staining
e. Lipid Staining
6. Cultivation of Microorganisms:
a. Preparation of Culture media-Nutrient Agar, Nutrient
Broth, MacConkey, Mannitol Salt Agar,
Sabouraud’sAgar
b. Inoculation techniques using Liquid media, Solid media-
Slant, Butt and Plate
7. Sterilization of Glassware and Culture media
a. Autoclave
b. Hot Air Oven
c. Bacteriological filter
8. Isolation of microorganisms
a. Streak Plate Method
b. Pour Plate method
c. Spread Plate method
9. Enumeration of Microorganisms
a. Viable count: Spread plate method and Pour plate
method
b. Haemocytometer
c. Breed’s Count Method
d. Opacity Tube Method
F.Y.B.Sc. Microbiology
Credit Based Semester & Grading System
2018-19
SEMESTER II Paper I
Course
Code
Title Credits
UGMB-
201
Biochemistry, Virology and Microbial ecosystem
1 Credits
(45 lectures)
Unit-I Basics of Biochemistry
1.1 DNA structure
a. Nitrogenous bases-Purines and Pyrimidines
b. Pentoses-ribose, deoxyribose
c. Nomenclature of nucleosides and nucleotides,
d. N-Beta glycosidic bond, polynucleotide chain to show
bonding between nucleotides (phosphodiester bonds)
1.2 Basic structure of RNA and DNA
1.3 Chemical foundation
a. Types of bonds and their importance: electrovalence,
covalent, ester, phosphodiester, thioester, peptide and
glycosidic bond
1.4 Water-Structure, properties in brief
1.5 Carbohydrates
a. Definition, Classification, Biological role
b. Monosaccharides
c. Oligosaccharides (Maltose, Cellobiose, Sucrose, Lactose)
d. Polysaccharides (Starch, Glycogen, Peptidoglycan,
Cellulose)
1.6 Lipids
a. Fatty acids as basic component of lipids and their
classification, nomenclature
b. Storage lipids and structural lipids
c. Types of Lipids with general structure of each and
mention examples
1.7 Amino acids and Proteins
a. General structure and features of amino acids (emphasis
on amphoteric nature)
b. Classification by R-group
c. Uncommon amino acids and their functions
d. Peptides and protein- Definition and general features and
examples with biological role
e. Primary, secondary, tertiary, quaternary structures of
proteins- Brief outline
15 Lectures
Unit-II Basic Virology
2.1 General Characteristics of Viruses
a. Viral structure
b. Nucleic acid, protein
c. Capsid and envelope
d. General Morphology:helical, polyhedral, enveloped,
complex
2.2 Classification of viruses (Baltimore classification)
2.3 Isolation, Cultivation and Multiplication of viruses
a. Bacteriophages: Lytic cycle, Lysogeny
b. Cultivation of bacteriophages (Phage Assay)
15 Lectures
Unit-III Microbial ecosystem 3.1 Principle of Microbial ecology
3.2 Ecological concepts
3.3 Ecosystem and biogeochemical cycling
3.4 Microbial Habitat
3.5 Environment and microenvironment
3.6 Biofilms: Microbial growth on surfaces, disadvantages and
control of biofilms
3.7 Freshwater, Soil and Plant microbial ecosystem
a. Freshwater Microbial ecosystems
b. Terrestrial Microbial ecosystems
c. Plants as microbial habitat
3.8 Animal-Microbial Symbiosis
3.9 Plant-Microbial Symbiosis
15 Lectures
F.Y.B.Sc. Microbiology
Credit Based Semester & Grading System
2018-19
SEMESTER II Paper II
Course
Code
Title Credits
UGMB-
202
Bioprocess Technology, Immunology and Medical
Microbiology
2 Credits
(45 lectures)
Unit-I 1.1 Introduction to Industrial Microbiology
Definition and scope of industrial Microbiology
1.2 Culture, components of media, types of media
1.3 Microbial Growth (Definition, Generation Time, Growth rate,
Phases of Growth, Chemostat, turbidostat)
1.4 Inoculum Development: Preparation of inoculum
1.5 Sterilization: media, equipment, fermentors
1.6 Fermentation: Types of fermentors, batch and continuous
fermentation, surface and submerged fermentation
1.7 Probiotics and prebiotics
15 lectures
Unit-II Basic Immunology
2.1 Introduction to Immunology: definition, types of immunity
innate- anatomic barriers, physiological and chemical barriers
and adaptive immunity, inflammation, Cells of Immune system-
- T cell, B cell, Natural Killer cell, Dendritic Cell,
Macrophages, Neutrophiles, Eosinophiles, Basophiles
2.2 Antigen: definition, types of antigen, epitope, hapten, adjuvant,
antigenicity and immunogenicity, foreignness of antigen
2.3 Antibody: definition, types, structure (diagram only) and
Antigen-Antibody reaction- Introduction to precipitation and
Agglutination
2.4 Types of Immune Response- Humoral and Cell Mediated
Immune Response
2.5 Primary and secondary lymphoid organs (Emphasis on
diagram)
15 lectures
Unit-III Infections
3.1 Infections: definition of medical terms, introduction,
classification of infection (primary and secondary) modes of
transmission of infection, sources of infection
3.2 Virulence Factors of pathogens: Enzyme, Toxins
3.3 Emerging Infections: Factors affecting emerging infections
3.4 Examples of Prevalent and Emerging Infections(Malaria, Ebola
Virus, Chikungunya, Leptospirosis, Opportunistic Infections by
E.coli, Nipah Virus, Swine flu)
15 lectures
F.Y.B.Sc. Microbiology Practicals
Credit Based Semester & Grading System
2018-19
Course
code
Title Credits
MB P2 Section II- Methods in Microbiology 2 Credit (45
Lectures)
1. Control of Microorganisms
a. Physical Methods - Temperature
b. Chemical Methods –Phenol, Antibiotic and Dyes
2. Effect of Different environmental conditions on growth of
Microorganisms
a. Temperature
b. pH
c. Oxygen (Static and shaker condition)
d. Osmotic Pressure (Salt and Sugar)
3. Isolation of Microbes from various sources- Soil, Water, Air,
Fomites, Fermented food
4. Study of viruses- Phage Assay
5. Qualitative detection of
a. Carbohydrates
b. Proteins
c. Amino acids
d. DNA
e. RNA
6. Study of Nitrogen fixers-Rhizobium and Azotobacter
7. Study of Normal flora of Skin
8. Detection of Virulence factors- Haemolysin , Coagulase and
Lecithinase
9. Detection of Blood group
10. Study of Malarial Parasite
11. Preparation of buffer and study of pH meter
12. Verification of Beer-Lambert’s Law by Colorimeter
References:
1. Madigan M.T., Martinko J.M. (2006). Brock’s Biology of Microorganisms. 11th Edition.
Pearson Education Inc.
2. Prescott L.M., Harley J.P., AND Klein D.A. (2005). Microbiology, 6th Edition.
MacGraw Hill Companies Inc.
3. Salle A.J. (1971) Fundamental Principles of Bacteriology. 7th Edition. Tata MacGraw
Publishing Co.
4. Stanier R.Y., Adelberg E.A. and Ingraham J.L. (1987) General Microbiology, 5th
Edition. Macmillan Press Ltd.
5. Tortora G.J., Funke B.R., Case C.L. (2006). Microbiology: An Introduction. 8th Edition.
Pearson Education Inc.
6. Kathleen Park Talaro & Arthur Talaro - Foundations in Microbiology International
edition 2002,| McGraw Hill.
7. Wilson K. and Walker J.M. (2005) Principles and Techniques of Biochemistry and
Molecular Biology. 6th Edition. Cambridge University Press.
8. Lehninger. Principles of Biochemistry. 4th Edition. D. Nelson and M. Cox. W.H.
Freeman and Company. New York 2005.
9. Microbiology: An Introduction. 6th Edition. Tortora, Funke and Case. Adisson Wesley
Longman Inc. 1998.
10. Manual of Microbiology: Tools & Techniques by Kanika Sharma 2nd edition.
11. General Microbiology by Powar & Daginawala Volume II.
12. A Textbook of Microbiology by R. C. Dubey & Maheshwari.
13. Microbiology by Jacquelyn Black 6 th edition.
14. Advanced Virology: Wagner 3 rd Edition.
15. Prescott, Hurley, Klein-Microbiology,9th edition, McGraw Hill. 9th Edition
16. Ananthanarayan and Panicker’s, Textbook of Microbiology, 9th edition
17. Kuby Immunology, 6th Edition, W H Freeman and Company
18. Casida L. E., "Industrial Microbiology”(2009) Reprint, New Age International (P) Ltd,
Publishers, New Delhi
19. Milk and Milk Products: Clarence Henry 4th Edition.