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Microscopy ListServer ArchivesFile Requested = 9502.txt
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From: Riitta Harjula: rharjula-at-cc.oulu.fi Date: Wed, 1 Feb 199514:38:54 +0200 (EET)
Contents Retrieved from Microscopy Listserver Archives
http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Subscribe Riitta Harjula

Riitta.Harjula-at-oulu.fi


3/125

From: Marc Brande: brande-at-sdsc.edu Date: Wed, 1 Feb 199511:39:27 -0800 (PST)
Subject: Confocal Scope in San Diego Contents Retrieved fromMicroscopy Listserver Archives

Microscopy List {microscopy-at-aaem.amc.anl.gov} ,
Confocal Microscopy List {confocal-at-ubvm.bitnet}
Message-Id:{Pine.3.05.1.9502011127.A7624-9100000-at-pauline.sdsc.edu}
Mime-Version:1.0
Content-Type: TEXT/PLAIN; charset=US-ASCII

I am in need of access toa confocal scope in the San Diego area. Would
anyone know of a lab that has oneand who to contact? Thanks in advance
for your help.

Marc

Marc C.Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group Tosubscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo Topost a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU Voice:619-587-4830


4/125

From: tivol-at-tethys.ph.albany.edu Date: Wed, 01 Feb 1995 16:45:52 EST
Subject: RE: EMLab scheduling ContentsRetrieved from Microscopy Listserver Archives

Jan Coetzee asks about electronic vs. "dog-eared diary" for

schedules.
Dear Jan,
We use a large desk calendar ourselves. It seemseasier, since there
are frequent changes, and everyone can check it instantly.I'm sure that a
calendar page on a computer might do as well, but when a usercalls, we are
not always booted up, so it would take us more time than it doeswith paper
and pencil. If your scopes are computer-controlled, it might savesome time
and/or effort to have schedules on the same computer--e.g. thecomputer could
dial a user you need to contact re schedule changes--but we havenot thought
this to be worthwhile for us. Sometimes low-tech works verywell!
Yours,
Bill Tivol


5/125

Subject:Peltier devices ContentsRetrieved from Microscopy Listserver Archives

Jan Coetzee inquired about mfgrs of pltrs.
Dear Jan,

Melcor makes them. Their address, phone & fax are
1040Spruce Street
Trenton NJ 08648-4587
UnitedStates of America (our USA)
(609) 393-4178 (phone)

(609) 393-9461 (fax)
One potential problem is that Peltiers have alimited delta-T, and can
get very expensive. I asked Melcor about setting up acold stage for epitax-
ial deposition in a vacuum, and there was nothing whichwould get cold enough.
Furthermore, if you wish to thermostat the device, youneed to use a cooler
which has a 100% duty cycle and output currentproportional to the difference
between target and actual temperatures. Wefound this out when we installed
Peltiers in our darkroom as heaters/coolers tomaintain developer temperature.
The intermittant current put out by the usualcommercially available devices
blew the Peltiers out! Otherwise, Peltiers aremarvelous devices. Good luck.
Yours,

Bill Tivol


6/125

From: WayneWNB-at-aol.com Date: Wed, 1 Feb 1995 22:28:40 -0500
Subject: Electron MicroscopePictures ContentsRetrieved from Microscopy Listserver Archives
Message-Id: {25020121201502-at-vms2.macc.wisc.edu}

G'day Subscribers...

I've received this request for help onlocating micrographs
of bacteria. I could not help this individual. So isthere
anyone out there that can?

Cheers....Nestor Z.
Your FriendlyNeighborhood SysOp
=====================================

P.S.

I'm working on concept of adding an Image Library to theSoftware
Library. When it's ready for contributions I'll post
a message totheListserver....

====================================

Ineed an electron microscope picture of Methanosarcina barkeriand
Methanobacterium wolfei. I called the University of California atBerkeley
and they gave me your address. They said that they don't keep a fileof
their past work and would charge $300 per picture to do the work again.I'm
fairly certain that the pictures already exist somewhere. I believe

that
NASA may have some of them but I don't know who to ask there . Thank youfor
any help in getting any pictures of the twobacteria.

------------------


7/125


8/125

From: Richard Lee: richard_lee-at-qmgate.anl.gov Date: 2 Feb1995 13:01:13 -0600
Subject: subscibe Contents Retrieved from Microscopy ListserverArchives

Message-Id: {n1420392126.63194-at-qmgate.anl.gov}

subscibe2/2/95

subscribe, PLEASE!
12:46 PM


9/125

From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood) Date: Fri, 3 Feb 1995 08:56:44+1100
Subject: TEM:Staining glycogen in sections Contents Retrieved from Microscopy ListserverArchives

Message-Id: {199502022051.JAA12437-at-arwen.otago.ac.nz}
X-Sender: st004718-at-brandywine.otago.ac.nz
Mime-Version: 1.0
Content-Type: text/plain; charset="us-ascii"

One of EM Unit users is studyingdeveloping Red deer. The samples we are
examining are skull and pedicle(developing antler) taken from the deer at
set time intervals over atwo yearperiod. The samples are processed
routinely, ie glutaraldehydefixed,decalcified, OsO4 post fixed, uranyl
acetate bloc stain, dehydrated andembedded in Agar 100 epoxy resin.

Our problem is that it now seems to bethat the glycogen content is of some
significance to the investigation.Unfortunately the above process is not
ideal for showing the glycogen.
Somerecently processed samples using potassium ferrocyanide with the
osmium arebrilliant however we would like to enhance the glycogen in the
previousblocks.
Does anyone out there know a method to enhance "unstainable" glycogen

in
ultrathin sections?
Thanks in anticipation.

AllanMitchell
South Campus EM Unit
allan.mitchell-at-stonebow.otago.ac.nz


10/125

From: randy nessler: rnessler-at-emiris.iaf.uiowa.edu Date: Thu,2 Feb 1995 14:51:56 -0600 (CST)
Subject: Site for JCPDS Contents Retrieved from Microscopy ListserverArchives

Does anyone know of a site that I can download filesfrom the JCPDS?
Randy Nessler
rnessler-at-emiris.iaf.uiowa.edu


11/125

From: Doug Arrell: ARRELL-at-jrc.nl Date: Fri, 3 Feb 199508:31:16 GMT+0200
Subject: Re: Convert PS files Contents Retrieved from Microscopy ListserverArchives

} I am looking for a way to convert my PostScriptfiles into "regular" image
} files. Does someone know of such siftwares oneither Mac or Unix platforms?
} Any information would be appreciated.
}
Do you mean postscript or encapsulated postscript? If postscript then
oneof the postscript interpreters available should do it. I use one
an a PCcalled GoScript that outputs to TIFF files as well as
printers. I am not sure,but you should take a look at the GNU
Ghostscript interpreter that runs onvirtually anything - certainly
on unix systems. If you mean encapsulatedpostscript (EPS) then just
import the files into a Mac word processor such asWordPerfect on
Microsoft Word and then copy and paste to whereever you wantto.
+------------------------------------+
| Dr Douglas Arrell|
| Mechanical Performance and Joining |
| Institute for Advanced Materials|
| 1755 ZG Petten |
| Netherlands

|
| {ARRELL-at-JRC.NL} |
| Tel. (+31) 2246 5287|
| Fax (+31) 2246 1917 |
+------------------------------------+


12/125

From: MicroToday-at-aol.com Date: Fri, 3 Feb 1995 08:58:20 -0500
Subject: Bacteria Micrographs Contents Retrieved fromMicroscopy Listserver Archives

Group -
Responding to an inquiry from Nestor, Custom

Medical Stock Photo is a
company which purchases micrographs and then sellsthem to publications, etc.
They have a considerable inventory - in microscopy,and a number "with"
bacteria.
Many readers may be interested in contactingthem and explore the sale of
their micrographs. I have, of course, nofinancial interest in the company.

Custom Medical Storck Photo,Inc.
3819 North Southport Ave
Chicago, IL 60613
Tel.: 312-248-3200
Fax: 312-248-7427

Regards,
Don Grimes, Microscopy Today


13/125

From: Kris_Kavanau-at-dmcmail.ucsf.edu Date: Fri, 03 Feb 1995 10:03:40 PST
Subject: UranylGlass/FM Stds. ContentsRetrieved from Microscopy Listserver Archives
Message-Id: {1995Feb03.100340.2874650620-at-dmcmail.ucsf.edu}

To: Microscopy-at-aaem.amc.anl.gov (M)

Subject:Time: 9:45 AM
OFFICE MEMO Uranyl Glass/FM Stds.Date: 2/3/95
Dear Microscopists,
Does anyone have any uranyl glass, or knowwhere it might be obtained? I
have been told that it is no longer manufacturedcommercially. It might be
an excellent "generic" fluorescence microscopycontrol.
Are there any commercially available, pre-mounted fluorescencestandards
besides "MultiSpeck" from Molecular Probes? They are veryconvenient, but
they are not ideal for our applications as DAPI, fluorescein,and Texas Red
specific controls. Unfortunately, Flow Cytometry Standards Co.no longer
makes pre-mounted standards.
I have been managing the UCSF coreflow and image cytometry facility ("Lab
for Cell Analysis") for 2 years, but Ihad no real QC for our 2
occasionally used fluorescence microscopes. Now Ineed to establish QC
protocols for 6 additional multi-user, computerized

fluorescence (one
scanning confocal) microscopes in the "National MolecularCytogenetics
Resource." I was surprised that so few standards (and journalreferences)
seem to be available.
Any suggestions or comments would begreatly appreciated. Thank you very
much. KrisKavanau; kavanau-at-dmc.ucsf.edu


14/125

From: John Kloetzel: kloetzel-at-umbc.edu (John Kloetzel) Date:Fri, 3 Feb 1995 14:14:17 -0500
Subject: EM network Contents Retrieved from Microscopy ListserverArchives

subscribe microscopy kloetzel-at-umbc.edu

** JAK**

****************************************************************************
John A. Kloetzel, Ph.D. {kloetzel-at-umbc.edu}
Department of Biological Sciences
University of Maryland Baltimore County (UMBC)
Catonsville, MD 21228-5329 USA
Phone: (410) 455-2247 or-3913 (Lab)
FAX: (410) 455-3875
****************************************************************************


15/125

From: Dr. William Dentler,University of Kansas, Dept. of Cell Biology, Date: Fri, 3 Feb 1995 15:24:59-0600
Subject: fix pepper redux Contents Retrieved from Microscopy ListserverArchives

Message-Id: {m0raUmA-00011cC-at-pegasus.cc.ucf.edu}

Afew months ago a thread ran on fixation and embedding pepper contaminant
artefact in biological ultrathin sections. A colleague of mine read the Pepper
Summary I compiled and sent me the following fixation protocols that he hasused
successfully without pepper. Notice the first one in which glutaraldehyde,
phosphate buffer AND OSMIUM are all mixed TOGETHER!

If anyone outthere wants a copy of my Pepper Summary, contact me off-line at my
e-mail and Iwill zip a copy out toyou.

------------------------------------------------------------------------

"1. For fixing cilia in mammalian trachea, I have used an "instantfixation"
method using a combination of osmium, phosphate buffer, andglutaraldehyde - in
the cold - for many years and have never seen the pepperdescribed in the Pepper
Summary you sent to me. Right - all that stuff in the

same buffer dumped on the
tissue. Works great, but may extract a bit of actin.

"The method I used was one described in a paper by Omnoto
and Kung inthe J. Cell Biol 87:33-46. I think it uses 50 mM NaPhosphate,
pH 7.2, 2% OsO4,2% glutaraldehyde. Add the Osmium just before you add
the tissue and fix onice for 10 min. If you want, you can remove the
black fix after 10 min and addanother slug of fix for another 10 min but
that is optional.

"Omoto andKung used it to fix Paramecium and their cilia. I have used it to
fixmammalian trachea (with their cilia). The advantage is that it seems
to"freeze" cilia in position, as opposed to glutaraldehyde, in which cells
actualy swim for a dab before either being fixed or dying (we fix cells,
we don't kill them, do we?). The osmium does not penetrate for more than
afew cell layers but, with epithelial tissue or single cells, it does
not makemuch difference.

"I have never tried that fix method on Chlamydomonas or

Tetrahymena. Over the
last year I have done a lot of embedding of Chlamy andhave never seen pepper.
For those beasts, I find that cacodylate gives thebest preservation of the
cytoplasm, although others find that phosphate bufferworks fine too.
I do fix with glutaraldehyde in culture medium (pretty muchphosphate buffer),
overnight in glut in cacodylate, rinse a few times incacodylate, then
into 0.5-1% OsO4 in cacodylate for 30-60 min on ice, rinsewith a few
changes of water and into uranyl acetate for a few hours prior todehydrating in
acetone and embedding in epon.

"I've also tried anothermethod in which you fix with glut in phosphate buffer,
rinse, then incubateovernight in uranyl acetate (in water) at 70 degrees
C. It works on Chlamy andavoids osmium. It is supposed to eliminate the
need for poststaining ofsections, but I did not find this to be the
case. I believe that I once readthat the reason for staining sections is
to put a dab of stain on structures

at the last surface of the plastic
that the beam sees before blasting throughthe objective lens. I don't
know, but, for Chlamy, I usually use the more oldfashioned method that I
gave you above. Pepper does not seem to be one of myproblems."
------------------------------------------------------------------------
Contributed to MICROSCOPY by:

--

Gib Ahlstrand, MMSNewsletter Editor
Electron Optical Facility, University of Minnesota, Dept.Plant Pathology
495 Borlaug Hall, St. Paul, MN 55108 (612)625-8249
612-625-9728 FAX, giba-at-puccini.crl.umn.edu


16/125

From: tivol-at-tethys.ph.albany.edu Date: Fri, 03 Feb 1995 18:23:23 EST
Subject: Re:TEM film ContentsRetrieved from Microscopy Listserver Archives

Dear Lucille,
I just got a number from Kodak for

technical questions, but they could
probably direct you to a distributer. Itis (800) 242-2424 x19. We usually
use a local vendor, National Graphics, but Ihave not noticed a great range
of prices with other vendors. Good luck.

Yours,
Bill Tivol


17/125

From: Dr. Edmund Glaser: eglaser-at-umabnet.ab.umd.edu Date: Fri, 3Feb 1995 22:12:08 -0500 (EST)
Subject: Re: TEM film Contents Retrieved from Microscopy ListserverArchives

I am not aware that "cheap" is an a.k.a. for "reliable".Please be kind
to the English language.

On Fri, 3 Feb 1995, Lucille A.Giannuzzi wrote:

} Can anyone recommend a reliable (a.k.a. cheap) U.S.vendor for TEM film?
}
} Thanks in advance,
} Lucille Giannuzzi
}
}
}*************************************************************************
}Lucille A. Giannuzzi, Ph.D.
}
} Dept. of Mechanical and Aerospace Eng.phone (407) 823-5770
} University of Central Florida fax(407) 823-0208
} 4000 Central Florida Blvd. email lag-at-pegasus.cc.ucf.edu
} Orlando, FL 32816-2450 USA
}*************************************************************************
}
}
}
}


18/125

From: Karpura V Kommineni: komminen-at-student.msu.edu Date: Sun, 5 Feb1995 12:32:08 -0500 (EST)
Subject: immunolabeling of wood Contents Retrieved fromMicroscopy Listserver Archives

Message-Id: {9502051732.AA72211-at-student1.cl.msu.edu}

DDoes anyone know about work done on immunolabelling of wood tissue offruit
trees. I'm interested in any kind of information I can get onfixation,
embedding and the labelling procedure. I plan to be using ProteinA-colloidal
gold to tag the antibody.I'll be using a confocal microscope for thisstudy.If
anyone knows any work done in this area could you please send methe
references. Thank you in advance.
my email address: komminen-at-student.msu.edu


19/125

From: Ian Hall: hall-at-me.udel.edu Date: Mon, 6 Feb 199508:31:26 -0500 (EST)
Subject: WDS Software Contents Retrieved from Microscopy ListserverArchives

Dear Fellow Microscopists,
We have recentlyacquired a scanning electron microscope with a
four crystal WavelengthDispersive Spectrometer but the associated
computer system is rather old andprobably not worth re-activating.
I know that there is software for theMacintosh, such as
"D.T.S.A." and "Flame", for ENERGY Dispersive Spectroscopybut my question
is "Is there any (Mac) software out there which can handleW(AVELENGTH)DS
spectral acquisition and processing?".
Any leads would bevery much appreciated. Thanks.

Rick Hall
Materials ScienceProgram
University of Delaware


20/125

From: Not Specified: Kris_Kavanau-at-dmcmail.ucsf.edu Date: Fri,03 Feb 1995 10:03:40 PST
Subject: Uranyl Glass/FM Stds. Contents Retrieved from Microscopy ListserverArchives

Reply to: RE} Uranyl Glass/FM Stds.
HiKris,
Uranium glass slides can be purchased from:

Newport IndustrialGlass, Inc.
1631 Monrovia Ave.
Costa Mesa, CA 92627
Tel: 714-642-9980
Fax: 714-645-6800
Contact person: Bill Larsen (you can tell him I sentyou).
Sold as a 6.5x6.5" sheet (you can specify 1 mm height), so you may wantto form
a "consortium" to have Newport pre-cut a sheet to slide size (nominalextra
cost, but your lab only needs one or two slides). If there is a lotof
interest, my company may start selling single slides.

As forreferences and the Shading Correction equation: please see my article in
the11/94 issue of Journal of NIH Research 6(11): 80 (usual Internet
disclaimer:yes, that is an ad from my company on the facing page). Also look
at Jericevicet al (1989) Methods in Cell Biology 30:47-83.

MutliSpeck beads: TheMolecular Probes pre-mounted slide kits should be ideal
for DAPI and

Fluorescein. I believe they were optimized for Rhodamine, but
should still workok for Texas Red. If your problem is with mounting, Mol.
Probes now sells thebeads in solution, so you can 'sprinkle' some on your
specimens. If you have adifferent problem with the current MultiSpeck's, Mol.
Probes may be able towork something out for you.

Sorry, but I usually buy my reference materialfrom Mol. Probes and don't keep
close track of other slidemanufacturers.

Sincerely,

Dr. George McNamara
Universal ImagingCorporation
George_M-at-Image1.com
--------------------------------------

Subject: Time: 9:45AM
OFFICE MEMO Uranyl Glass/FM Stds. Date:2/3/95
Dear Microscopists,
Does anyone have any uranyl glass, or know whereit might be obtained? I
have been told that it is no longer manufacturedcommercially. It might be
an excellent "generic" fluorescence microscopy

control.
Are there any commercially available, pre-mounted fluorescencestandards
besides "MultiSpeck" from Molecular Probes? They are veryconvenient, but
they are not ideal for our applications as DAPI, fluorescein,and Texas Red
specific controls. Unfortunately, Flow Cytometry Standards Co.no longer
makes pre-mounted standards.
I have been managing the UCSF coreflow and image cytometry facility ("Lab
for Cell Analysis") for 2 years, but Ihad no real QC for our 2
occasionally used fluorescence microscopes. Now Ineed to establish QC
protocols for 6 additional multi-user, computerizedfluorescence (one
scanning confocal) microscopes in the "National MolecularCytogenetics
Resource." I was surprised that so few standards (and journalreferences)
seem to be available.
Any suggestions or comments would begreatly appreciated. Thank you very
much. KrisKavanau; kavanau-at-dmc.ucsf.edu


21/125

From: SiSTek-at-aol.com Date: Mon, 6 Feb 1995 13:53:07 -0500
Subject: subscribe Contents Retrieved from Microscopy ListserverArchives

Subscribe, please.

Thanks,

Mark Anderson,

SiSTek


22/125

From: Deborah Holmberg: dlholmberg-at-ucdavis.edu Date: Mon, 6 Feb1995 11:01:33 -0800 (PST)
Subject: Scanning 95 meeting Contents Retrieved from Microscopy ListserverArchives

The organizers of the 28-31 March 1995 Scanning 95 meetingwant about 25
student volunteers to run the slide projectors for the sessionsin
exchange for full meeting registration ($275). Please contact:
Mary K.Sullivan
FAMS, Inc
P.O. Box 832
Mahwah, New Jersy
07430,0832

or leave me a message.
Debe Holmberg e-mail {dlholmberg-at-peseta.ucdavis.edu}
Lab 916-752-9021
FAX 916-752-4604


23/125

From: David Leaffer(415)852-1828 : David.Leaffer-at-syntex.com Date: 06 Feb 1995 11:56:02 -0800 (PST)
Subject: TEM Calibration Contents Retrieved fromMicroscopy Listserver Archives

Return-receipt-to: David.Leaffer-at-syntex.com
Registered-mail-reply-requested-by: David.Leaffer-at-syntex.com

I am going to beworking on an TEM project that will be under "GLP" . GLP are
guidlines fordoing certain experiments for the FDA (I think the equivalent in
Europe is ISO9000). I was wondering if anyone out there is doing TEM under
these guidlines?And if so what are they using for magnification calibration.
I do not believethat there are any vendors who can supply a certified
magnification standardfor TEM, which, I think is required for GLP.

Thanks,
DavidLeaffer
Syntex Research
david.leaffer-at-syntex.com


24/125

From: Deborah Holmberg: dlholmberg-at-ucdavis.edu Date: Mon, 6 Feb1995 16:38:54 -0800 (PST)
Subject: Scanning 95 meeting Contents Retrieved from Microscopy ListserverArchives

The organizers of the 28-31 March 1995 Scanning 95 meetingwant about 25
student volunteers to run the slide projectors for the sessionsin
exchange for full meeting registration ($275). Please contact:
Mrs.Mary K. Sullivan
FAMS. Inc.
P.O. Box 832
Mahwah, NJ 07430-0832

or leave me a message.
Debe Holmberg
Lab 916-752-9021
Fax 916-752-4604
dlholmberg-at-peseta.ucdavis.edu


25/125

From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood) Date: Wed, 8 Feb 1995 13:50:28+1100
Subject: Academic role Contents Retrieved from Microscopy ListserverArchives

Dear All,
We are are multi-user electron microscopefacility which has an extensive
range of equipment and uses a wide range oftechniques.
Five technical staff from three contributing University departmentsare
employed full-time to undertake for work coming from both insideand
outside the University.

The role of the 'academic in charge' of thefacility is shortly to come up
for reassessment and so it is a pertinent timefor us to reconsider what
that role should entail.
We are looking forfeedback from other individuals as to what they see the
contribution of anacademic in this environment should be.
Any opinions/suggestions?


26/125

From: Nestor J. Zaluzec-Argonne Nat. Lab. : ZALUZEC-at-AAEM.AMC.ANL.GOV Date: Tue, 7 Feb 1995 21:59:52 -0600 (CST)
Subject: Post Doc InTribology ContentsRetrieved from Microscopy Listserver Archives

From: wabutter-at-ix.netcom.com (Wayne A. Buttermore) Date: Tue, 7 Feb 1995 20:03:15 -0800
Subject: subscribe Contents Retrieved from Microscopy Listserver Archives
Message-Id: {9502071626.AA16923-at-riker.ml.wpafb.af.mil}

Subscribe


27/125

From: Peter Goodhew: goodhew-at-liverpool.ac.uk Date: Wed, 8 Feb1995 08:43:25 GMT
Subject: Re: Academic role Contents Retrieved from Microscopy ListserverArchives

} The role of the 'academic in charge' of thefacility is shortly to come up
} for reassessment and so it is a pertinenttime for us to reconsider what
} that role should entail.
} We are lookingfor feedback from other individuals as to what they see the
} contribution ofan academic in this environment should be.
Easy -
1. Keep abreast of alltechniques which a) you have, b) exist, and c) potentially exist.
2. Be anexpert practionioner of two or more of these. Publish a lot in your ownname.
3. Give advice on the application of all techniques and on the high-levelinterpretation of all results. Publish
jointly with others.
4. Raise fundsto replace equipment and to buy new techniques as they become applicable.
5.Make sure all users publish, and tell you about it!
6. Establish a reputationas a scientist in some major subject area, not just as a microscopist. Publish alot.
7. Keep friendly with the heads (or budget controllers) of all potential

user departments.
8. Get to know lots of people in your institution by playingsport/drinking/etc with them.
9. In your spare time, publish somemore.

I offer this advice after 20 years of running such afacility!

PeterGoodhew

----------------------------------------------------------------------------------------------------------
Professor Peter J Goodhew,Department of Materials Science & Engineering
University of Liverpool
LIVERPOOL Fax (44) (0)51 794 4675
L69 3BX, UKTel (44) (0)51 794 4665 (secretaryDebra)
----------------------------------------------------------------------------------------------------------
inter alia: Director of the MATTER projectfor educationalsoftware
----------------------------------------------------------------------

------------------------------------


28/125

From: Philip.P.Edge-at-ioppl.co.uk (Tel 0272 297481) Date: Wed, 8 Feb 1995 11:11:01 +0000
Subject: Subscribe Contents Retrieved from Microscopy Listserver Archives
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29/125

From: Philip.P.Edge-at-ioppl.co.uk (Tel 0272 297481) Date: Wed, 8 Feb 1995 11:14:29 +0000
Subject: Bioimaging Contents Retrieved from Microscopy Listserver Archives
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BS1 6NX England Telex: 449149 INSTPG

E-mail Contact Details
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Janet : {mailbox} -at-uk.co.ioppublishing
X400 : /s= {mailbox}/o=ioppl/prmd=iopp/admd=0/c=gb/

IOPP Internetservices
----------------------
Gopher : gopher.ioppublishing.com
WWW

Url : http://www.ioppublishing.com

Dear Moderator

I am thePublisher of the journal Bioimaging which I hope you have heard
of. I wouldlike to place information about the journal, including tables
of contents, onyour bulletin board. Will this be possible and how should I
do it?

Bestwishes, Philip Edge.


30/125

From: jester-at-crnjjsgi.swmed.edu (James V. Jester) Date: Wed, 08 Feb 1995 12:10:01 -0600
Subject: Lab Design Contents Retrieved from Microscopy Listserver Archives

Several years ago there appeared an article in

Science discussing
laboratory space design and a recent "New" model beingimplemented in
England. Does anyone remember this article, and what hashappened
to the English experiment?


31/125

From: Marc Brande: brande-at-sdsc.edu Date: Wed, 8 Feb 199510:04:15 -0800 (PST)
Subject: Timelapse Cell Culture Movies Contents Retrieved fromMicroscopy Listserver Archives

Functional Neuroimaging List {lat-at-po.cwru.edu} ,
ConfocalMicroscopy List {confocal-at-ubvm.bitnet} ,
Cell Bio List {cellbiol-at-net.bio.net}
Message-Id: {Pine.3.05.1.9502081015.B14211-a100000-at-pauline.sdsc.edu}
Mime-Version: 1.0

Can Anyone point me to sources (free to minimal cost) of analog (VCRtape)
or digital timelapse movies of cells in culture? This is notfor
commercial use, only presentation demonstration. Of course I wouldcredit
each source in the presentation. Thanks in advance for any help you cangive.

Marc

Marc C. Brande, M.S. SD3D Email List:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDU

Voice:619-587-4830


32/125

From: evansnd-at-ornl.gov(Neal D. Evans) Date: Wed, 8 Feb 1995 13:17:21 -0500
Subject: Subscribe Contents Retrieved fromMicroscopy Listserver Archives

Subscribe Microscopy evansnd-at-ornl.gov

Dr. Neal

D. Evans
Shared Research Equipment Program
Oak Ridge NationalLaboratory
Building 5500, MS 6376, Oak Ridge, TN 37831-6376
voice(615-576-4427) fax(615-574-0641) email(evansnd-at-ornl.gov


33/125

From: stuw-at-lanl.gov(Stuart Wright) Date: Wed, 8 Feb 1995 11:22:29 -0700
Subject:Reconditioned #SEMs Contents Retrieved from Microscopy Listserver Archives
Message-Id: {9502081818.AA09732-at-mustang.mst6.lanl.gov}

X-Sender: stuw-at-mustang.mst6.lanl.gov
Mime-Version: 1.0
Content-Type:text/plain; charset="us-ascii"

Is anyone aware of a company that sellsreconditionedSEMs?

+---------------------------------------------------------+
|Stuart Wright Los Alamos National Lab, MST-6 |
|.........................................................|
| Mail StopG770 phone: (505) 665-3647 |
| Los Alamos, NM 87545 fax:(505) 667-5268 |
+---------------------------------------------------------+


34/125

From: Daniel E. Sampson: des-at-rupture.ucsc.edu Date: Wed, 8 Feb1995 11:22:29 -0700
Subject: Reconditioned SEMs Contents Retrieved from Microscopy ListserverArchives

Is anyone aware of a company that sells reconditionedSEMs?

+---------------------------------------------------------+
|Stuart Wright Los Alamos National Lab, MST-6 |
|.........................................................|
| Mail StopG770 phone: (505) 665-3647 |
| Los Alamos, NM 87545 fax:(505) 667-5268 |
+---------------------------------------------------------+

------ Forwarded message ends here ------

*******************************************************************
Daniel E. Sampson dsampson-at-earthsci.ucsc.edu
Instrumentation Specialist Phone: (408) 459-4992
Earth Sciences FAX: (408) 459-3074
University of California
Santa Cruz, CA

95064
*******************************************************************


35/125

From: Elinor Solit: cambrex-at-world.std.com Date: Wed, 8 Feb1995 17:34:11 +0001 (EST)
Subject: Re: Reconditioned #SEMs Contents Retrieved fromMicroscopy Listserver Archives

Stuart,
Have you tried contacting the instrumentmanufacturers themslves? I
believe that JEOL and Hitachi, for instance, maysell the
reconditioned SEMs that comes in on trade-ins. Failing that, give
us a call, we'll try to direct you to more sources.

Ellie Solit,
Publisher of MICROSCOPE TECHNOLOGY & NEWS AND
THE MICROSCOPEBOOK

On Wed, 8 Feb 1995, Stuart Wright wrote:

} Is anyone awareof a company that sells reconditioned SEMs?
}
}
}+---------------------------------------------------------+
} | Stuart WrightLos Alamos National Lab, MST-6 |
}|.........................................................|
} | Mail Stop G770phone: (505) 665-3647 |
} | Los Alamos, NM 87545 fax: (505)667-5268 |
}+---------------------------------------------------------+
}
}


36/125

From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood) Date: Thu, 9 Feb 1995 16:43:19+1100
Subject: Academic role Contents Retrieved from Microscopy ListserverArchives

Message-Id: {199502090437.RAA16024-at-arwen.otago.ac.nz}
Mime-Version: 1.0

Dear All,
Further to my message of8.2.95, thank you very much to those who have
responded so candidly to myrequest for people's feelings and experiences
on this topic. I appreciate thatit can be a sensitive issue, not helped
when one is replying to someone whodoesn't even identify themselves
properly.
As I neglected to state who I amand where I work (I changed computers days
ago and forgot to put the automaticsignature on - the ramifications of
this I am just becoming aware of...) thatinfo now follows.
Maybe now I'll get some more replies...

RichardEasingwood
South Campus Electron Microscope Unit
Otago Medical School
POBox 913
Dunedin
NEW ZEALAND

Telephone: 64-03-479 7301
Facsimile:64-03-479 7254


37/125

From: Kathy Walters: kwalters-at-emiris.iaf.uiowa.edu Date: Thu,9 Feb 1995 07:17:27 -0600 (CST)
Subject: Neg stain of lipids Contents Retrieved fromMicroscopy Listserver Archives

Dear Fellow Microscopists,

I have been asked to doparticle sizing of a 10% fat immulsion (pH 8.0)
and had hoped to be able to doa very quick negative staining procedure as
this may need to be run frequently.I toyed with the idea of freeze
fracture, but the time involved is notconvenient for lots of runs. Has
anyone tried this? What stains would yousuggest? Is Osmium
vaper fixation nessecary?

Thanks for anyhelp!
Kathy Walters


38/125

From: stuw-at-lanl.gov(Stuart Wright) Date: Thu, 9 Feb 1995 09:06:22 -0700
Subject:reconditioned SEMs Contents Retrieved from Microscopy Listserver Archives
Message-Id: {199502091342.AA01519-at-mail.mmmg.com}

Is

anyone aware of a company that sells reconditionedSEMs?

+---------------------------------------------------------+
| Stuart Wright Los Alamos National Lab, MST-6 |
|.........................................................|
| Mail StopG770 phone: (505) 665-3647 |
| Los Alamos, NM 87545 fax:(505) 667-5268 |
+---------------------------------------------------------+


39/125

From: Jay Jerome: jjerome-at-isnet.is.wfu.edu Date: Thu, 9 Feb1995 11:10:54 -0500 (EST)
Subject: Re: Neg stain of lipids Contents Retrieved fromMicroscopy Listserver Archives

We have considerable experience with negative stain ofphospholipid
vesicles, lipoproteins, and triacylglyceride emulsions. In these
circumstances fixation is not critical, and in fact can produce
artefacts.PTA stain seems to work best. We concentrate our sample on
grid by dryingmultiple drops under gentle nitrogen gas stream prior to
negative stain. Thisavoids clumping in the suspension. Some sizing
artefacts can occur as dryedparticles of large size can deform slightly.
See Ganz et al, 1991, J Lipid Res31:163 for nice discussion of this.
Good luck-

JayJerome
**************************************************************
* aka:W. Gray Jerome *
* Dept. of Pathology*
* Bowman Gray School of Medicine of Wake Forest University *
* MedicalCenter Blvd *
* Winston-Salem, NC 27157-1092 *
* 910-716-4972

*
* jjerome-at-isnet.is.wfu.edu*
**************************************************************

On Thu,9 Feb 1995, Kathy Walters wrote:

} Dear Fellow Microscopists,
}
}I have been asked to do particle sizing of a 10% fat immulsion (pH 8.0)
} andhad hoped to be able to do a very quick negative staining procedure as
} thismay need to be run frequently. I toyed with the idea of freeze
} fracture,but the time involved is not convenient for lots of runs. Has
} anyone triedthis? What stains would you suggest? Is Osmium
} vaper fixationnessecary?
}
} Thanks for any help!
} Kathy Walters
}
}
}


40/125

From: tivol-at-tethys.ph.albany.edu Date: Thu, 09 Feb 1995 12:06:33 EST
Subject: Re:Neg stain of lipids Contents Retrieved from Microscopy Listserver Archives

Dear Kathy,
Staining is not really my field, but I'd

suggest a water-soluble heavy
metal and NO osmium. The Os would only dissolvein the lipid and reduce con-
trast. If possible, looking at a frozen, hydrated(or lyophyllized in-situ)
specimen would be best. You don't specify either thematrix of the emulsion
(I assume aqueous) nor the technique to be used (Iassume TEM), but a possible
protocol would be to add the stain to the emulsionand rapidly freeze, then
cryo-section, examine on a Friday, raise the temp to~-90C, come in Saturday
and raise the temp to -80C, Sunday to -70C, and look atthe freeze-dried spec-
imen Monday. If you can do the particle sizing from thefrozen-hydrated spec-
imen, the last steps can be omitted. Keeping the stainstrictly in the aqueous
phase should prevent size changes, etc. in the lipiddrops. Good luck.
Yours,
BillTivol


41/125

From: jacobb-at-ux5.lbl.gov Date: Thu, 9 Feb 1995 13:50:06 -0800
Subject: Re: Reconditioned SEMs Contents Retrieved fromMicroscopy Listserver Archives

Stuart,

E.J. Fjeld Co,
3 Executive Park

Drive
North Billerica MA 01862
Phone 508-667-1416

Providesreconditioned AMRAY microscopes. They also make special stages and
accessories.They've been around for a long time and are reliable.

Jacob Bastacky,MD
1-116
Lawrence Berkeley Laboratory EMail: sjbastacky-at-lbl.gov
University of California Phone: (510) 486-4606
Berkeley, California 94720 Fax: (510) 486-4750


42/125

From: Elinor Solit: cambrex-at-world.std.com Date: Thu, 9 Feb1995 15:20:13 +0001 (EST)
Subject: Re: reconditioned SEMs Contents Retrieved fromMicroscopy Listserver Archives

Stuart, my attempts to reply to your question by email haveresulted in 6
notices of undelivered mail. I left a voice message on yourphone this
morning. I think we can help you in yoursearch.

Regards,
Ellie Solit, Publisher/Executive Editor of MicroscopeTechnology & News
and The Microscope Book, a Smart catalog.

On Thu,9 Feb 1995, Stuart Wright wrote:

} Is anyone aware of a company that sellsreconditioned SEMs?
}
}
}
}+---------------------------------------------------------+
} | Stuart WrightLos Alamos National Lab, MST-6 |
}|.........................................................|
} | Mail Stop G770phone: (505) 665-3647 |
} | Los Alamos, NM 87545 fax: (505)667-5268 |
}+---------------------------------------------------------+
}
}


43/125

From: Jean Armour Polly: jpolly-at-nysernet.ORG Date: Thu, 9 Feb 199513:00:28 -0500
Subject: Apple QuickTime Conferencing Contents Retrieved from Microscopy ListserverArchives

Message-ID: {95020916094142E.RQDA-at-USCN.USCN.UGA.EDU}(UMass-Mailer 4.04)
Neuroscience List {neur-sci-at-net.bio.net} ,
Digital Video List{digvid-l-at-ucdavis.edu} ,
Confocal Microscopy List {confocal-at-ubvm.bitnet} ,
Cell Bio List {cellbiol-at-net.bio.net}
Message-Id:{Pine.3.05.1.9502091131.A22203-e100000-at-pauline.sdsc.edu}
Mime-Version:1.0

I thought this postshould be quickly disseminated.

Marc C. Brande, M.S. SD3D EmailList:3D Imaging
San Diego 3D Imaging Group To subscribe/unsubscribe:listserv-at-bobcat.etsu.edu
3840 Camino Lindo To post a message:sd3d-at-bobcat.etsu.edu
San Diego, CA 92122 Email:BRANDE-at-SDSC.EDUVoice:619-587-4830

---------- Forwarded message ----------

THEFOLLOWING RELEASE MOVED OVER PR NEWSWIRE ON TUESDAY, FEBRUARY 7, 1995 AT
11:42

AM, PST.

Contact:
Julie Karbo
Stirling & Cohan
(415) 513-0974
e-mail: jkarbo-at-applelink.apple.com

Brooke Cohan
Stirling &Cohan
(415) 513-0973
e-mail:cohan-at-applelink.apple.com

AppleAnnounces QuickTime Conferencing
Open, Cross Platform Conferencing,Collaboration and Multimedia
Communications Technology

SAN FRANCISCO,California--February 7, 1995--Apple Computer today
announced a cross platformconferencing, collaboration and
multimedia communications technology thatallows personal computer
users to share real-time information, images and soundanywhere in
the world. Apple is currently making the technology,called
QuickTime Conferencing, available to corporate allies who planto
create or have announced they are creating end user applications
based onthe technology. QuickTime Conferencing is a standards-
based architecture thatallows users to:

-- video conference and collaborate--to share and annotate

text,
images, screen capture, sound, video and virtual scenes real-time
among fellow conference participants in a variety oflocations
worldwide. QuickTime Conferencing allows users torecord
conversations and transform those conversations intoQuickTime
movies. All of this can be done on a variety of networks suchas
an Integrated Services Digital Network (ISDN), the worldwide
internet,local area and wide area networks and Asynchronous
Transfer Mode (ATM)networks. QuickTime Conferencing can be used
by a number of simultaneoususers, the total number being only by
available network bandwidth.

--conduct cross platform video conferencing connectivity
between Macintoshcomputers, PCs, UNIX systems and room-based
conferencing systems through theuse of the H.320 worldwide
teleconferencing standard.

-- broadcast andview multimedia content--digital audio, music
and video on a local or wide area

network.

Through alliances QuickTime Conferencing technology is expectedto
yield product bundles such as:
-- Apple Media Conference Kit--Consistingof the QuickTime
Conferencing system extension, the Apple MediaConference
application and a high quality, color video camera.
-- AppleMedia Conference Pro Kit--Consisting of the QuickTime
Conferencing systemextension, the Apple Media Conference
application, a color video camera and anH.320 codec/ISDN adapter
board. Being developed by Sagem/SAT, a leadinginternational
communications product company, the board is designed toallow
interoperability between platforms (Power Macintosh to Macintosh,
PC,UNIX and room systems) and full-screen image sharing.
--Complete MediaConference System--Consisting of an Apple Media
Conference Kit, a Power


44/125

Macintosh 7100 AV, a 17 inch color
monitor, external speakers and akeyboard.

Because QuickTime Conferencing is software-based, it iseasily
incorporated into new and existing third party products. Assuch,
Apple believes that QuickTime-compatible products couldyield
extremely affordable prices:
-- Apple Media Conference Kit--under$200
-- Apple Media Conference Pro Kit--under $1,750
-- Complete MediaConferencing System--under $6,000

Apple is working with a wide range ofcompanies including telcos,
network, software and hardware providers and

developers to provide
a range of solutions that take advantage of the benefitsof
QuickTime Conferencing (see associated releases). These allies
haveannounced that they expect to make products available in the
second quarter of1995.
From the home office to university campuses to themultinational
enterprise network, QuickTime Conferencing will allow usersto
communicate with people across the country or across the world.
Userswon't have to worry about whether their hardware equipment,
networkingequipment and applications are compatible with the
solutions being used on theother end of the network line.
QuickTime Conferencing is designed to be fullyoperational with
H.320 standards-based systems.
"The introduction ofQuickTime Conferencing will not only extend
Apple's leadership in multimedia,but will make an important
difference in the video conferencing andcollaboration market,"
said Rick Shriner, vice president of Apple's Core

Technologies
Group. "Our goal in designing QuickTime Conferencing wasto
develop a solution that allowed people the opportunity to
communicate andcollaborate. By making it open in every sense of
the word, our users canmetaphorically break down the walls of
their homes, schools and offices andexpand the boundaries of
their lives."
QuickTime Conferencing users canhave access to people,
information, sights and sounds that could never becombined
before. For example:
-- An author in Tokyo, Japan and herpublisher in San Francisco,
California can view and discuss cover art for a newnovel. They
can each view the design at several different angles,change
the visual perspective of the artwork, and annotate the imageand
accompanying text for the other to see.
-- A sixth grade class inDallas, Texas can discuss and view the
effects of global warming with anenvironmental scientist at U.C.
Berkeley's Lawrence Labs in California by using

QuickTime
Conferencing over the internet.
-- A special effects producer inHollywood, California can take a
movie director on the East Coast through avirtual tour of a
proposed set design. While the producer records theirdiscussion
as a QuickTime movie, the director can pan around the scene,zoom
in to look at props and view the set design from a varietyof
angles.
-- A breast cancer patient and her doctor in Fargo, NorthDakota
can consult with a leading oncologist in Boston, Massachusetts on
herprognosis and course of treatment. The Boston physician can
view hermammograms and annotate her medical chart as they
converse.
-- A CEO'scompany-wide address can be broadcast for easy viewing
by all employees attheir personal desktop.

Because QuickTime Conferencing allows for sharingof multimedia
data and reduces the time and expense of travel, it allowspeople
to be more productive than ever before.
"In the past people found

video conferencing easy to resist
because prices were high and the number ofpeople they could
communicate with was extremely limited," said RickLeFaivre,
senior vice president of the Apple Technology Group. "Nowfor
what we expect to be very aggressive prices, people can conduct a
mediaconference with virtually anyone, anywhere in the world. A
Power MacintoshQuickTime Conferencing user can share QuickTime VR
(virtual reality) images,annotate text documents and share digital
music over networks from basic rateISDN to the internet to ATM."
Because QuickTime Conferencing is a software-based architecture,
application developers, communications providers andhardware
vendors can easily develop compatible solutions. Forexample,
Crosswise Corporation, the maker of Face to Face, a cross-


45/125

platform
document conferencing application, developed aQuickTime
Conferencing-compatible version of their software in justone
month. A QuickTime Conferencing compatible application sharesthe
interface of other QuickTime Conferencing-enabled thirdparty
applications, so customers can begin using applications quickly
andeasily.
QuickTime Conferencing is based on Apple's award winningQuickTime
technology. It is a conferencing architecture whichallows
support for both industry standards such as H.320, as well

as
proprietary architectures, and codecs such as Indeo by Intel
Corporation.QuickTime Conferencing is transport, compression and
media-device independent.Apple's built-in AV capabilities
combined with the performance of the PowerPCRISC architecture,
make it easy for users to make multimedia connections withothers
on the information superhighway almost as soon as they pull
QuickTimeConferencing out of the box.
"Having QuickTime Conferencing available in myhome, office, and
studio literally allows me to be in multiple locations atone
time--it's the next best thing to having a Star Trek transporter,"
saidLos Angeles-based screenwriter and multimedia special effects
consultantMichael Backes, co-author of the screenplay for
Jurassic Park and other motionpictures. "Within the next few
months, I'll be counting on QuickTimeConferencing as the backbone
for my business."
"The short and sweet ofQuickTime Conferencing is that it requires
less network bandwidth and uses

innovative technology," says Matt
Ghourdjian, National Director of Technologyat Howrey & Simon, a
300-lawyer law firm serving Fortune 50 clients. Howrey &Simon
intends to use the product to send QuickTime movies of depositions
andre-enactments for lawyers to use in court; for live document sharing;
forconsultation between partners; and to conduct tours of the firm's
Washington,DC office from Los Angeles. "It's simply outstanding," says
Chris Masten,Howrey & Simon's Technical Litigation Support Manager.
To use the AppleMedia Conference Kit on the Macintosh, users need
at least 16 Megabytes of RAM,a 68040 or PowerPC-based Macintosh,
System 7.5, a network interface such asEthernet, ISDN, Token
Ring, and optionally the ability to digitize audio andvideo using
the built-in AV subsystem or a third party digitizer card. Touse
the Apple Media Conference Pro Kit on Macintosh, users need at
least 16Megabytes of RAM, an AV PowerPC-based Macintosh and an
ISDN connection. To

communicate with QuickTime Conferencing users
from the PC and other platforms,users will need an H.320
compatible codec on their machine, available from avariety of
vendors. QuickTime Conferencing technology is currentlyunder
development and products using the technology have not yetbeen
completed. Apple will provide pricing and availability
informationwhen products are completed and ready for release.
Apple Computer, Inc., arecognized pioneer and innovator in the
information industry, creates powerfulsolutions based on easy to
use personal computers, servers, peripherals,software, online
services, and personal digital assistants. Headquarteredin
Cupertino, California, Apple (NASDAQ:AAPL) develops,manufactures,
licenses and markets products, technologies and services forthe
business, education, consumer, scientific & engineering and
governmentmarkets in over 140 countries.

-30-

Apple, the Apple logo,

QuickTime and Macintosh are registered
trademarks and Power Macintosh is atrademark of Apple Computer,
Inc. Additional company and product names may betrademarks or
registered trademarks of the individual companies andare
respectfully acknowledged.

END

Applelink pathway:
NewsBreak
Apple & Industry News
PR Express
Apple PressReleases
2/7/95


46/125

From: Dascorr-at-aol.com Date: Fri, 10 Feb 1995 10:38:26 -0500
Subject: Reconditioned SEMs Contents Retrieved fromMicroscopy Listserver Archives

I know of a company that reconditions old AMRAY SEMs. The

owner used to work
at AMRAY before starting his own company. Company is E.J.Feld located in
Massachusetts. I can give you the phone on Monday, 2/13 when Ireturn to
work.
Dr. David A. Shifler
Powell Labs Ltd.
Baltimore, MD31231
(410) 327-3500
(410) 327-7506 (FAX)


47/125

From: Liang, Long: LLIANG-at-is.Arco.COM Date: 10 Feb 199511:07:11 CST
Subject: Books-- EM & Geology ? Contents Retrieved from Microscopy ListserverArchives

Message-Id: {MACMS.LLIANG.061405110095041FMACMS-at-IS.ARCO.COM}

Dear Microscopists,

Does anyone know if there isany recent books about applications of
microbeam techniques to mineralogy andpetrology?

The one I have was published by Mineralogical Association ofCanada
(Short Course in Microbeam Techniques) in May 1976.

Thanks inadvance.

Long LIang
ARCO EPMA/SEM Lab
PLano, TX


48/125

From: JoRita Jordan: jjordan-at-world.std.com Date: Fri, 10 Feb1995 13:27:19 +0001 (EST)
Subject: TEM comments sought Contents Retrieved from Microscopy ListserverArchives

TEM users:

Thanks to all the replies to my recentTEM survey, I am well on my way to
preparing the survey for publication. I needsome information -- I'm a
chemist, not a microscopist -- What do TEM users seeas important trends in
TEM? New technology? Important applications? Is thereother technology that
is replacing TEM in any way. How important areaccessories like EDS and
EELS? For what uses?

Any comments on today'sTEM would be greatly appreciated -- I'll send a copy
of the survey to anycontributor.

Jo Rita Jordan
Analytical Consumer


49/125

From: DCROMEY-at-CCIT.ARIZONA.EDU Date: Fri, 10 Feb 1995 13:50:01 -0700 (MST)
Subject:Phone # for Presetation Technol. needed Contents Retrieved from Microscopy ListserverArchives

Does anyone in the group have the phone number forPRESENTATION
TECHNOLOGY? We are in the information gathering stage for thepurchase
of a 35mm slide maker and the phone number we have (408-774-3733) isnot
correct.

Thanks for your help.

Doug

Douglas W.Cromey, M.S.
Cell Biology and Anatomy
Arizona Health Sciences Center

1501 N. Campbell Ave.
Tucson, AZ 85724
(602)626-2824dcromey-at-ccit.arizona.edu


50/125

From: Peter D. Barnett: pbarnett-at-crl.com Date: Fri, 10 Feb 199522:41:51 -0800 (PST)
Subject: Phone # for Presetation Technol. needed Contents Retrieved fromMicroscopy Listserver Archives

help

Peter D. Barnett - Forensic Science Associates- Richmond CA
pbarnett-at-crl.com VOICE: 510-222-8883 FAX: 510-222-8887


51/125

From: timonf-at-earth.ruu.nl(Timon Fliervoet) Date: Mon, 13 Feb 1995 08:24:35 +0100
Subject: Re:Books-- EM & Geology ? Contents Retrieved from Microscopy Listserver Archives
Message-Id: {n1419587852.54350-at-qmgate.anl.gov}

}

Dear Microscopists,
}
} Does anyone know if there is any recent books aboutapplications of
} microbeam techniques to mineralogy and petrology?
}
}The one I have was published by Mineralogical Association of Canada
} (ShortCourse in Microbeam Techniques) in May 1976.
}
} Thanks in advance.
}
} Long LIang
} ARCO EPMA/SEM Lab
} PLano, TX

Try:
McLaren,A.C., 1991, TEM of minerals and rocks, Cambridge UniversityPress,
Cambridge

Boland, J.N. & FitzGerald, J.D. (eds), 1993, Defectsand processes in the
solid state: geoscience applications, Developments inPetrology, Vol 14,
Elsevier, Amsterdam

Buseck, P.R. (ed), 1992, Mineralsand reactions at the atomic scale: TEM,
Mineralogical Society of America,Reviews in Mineralogy, vol 27.

CheersTimon

------------------------------------------------
Timon Fliervoet,Timonf-at-earth.ruu.nl, Faculty of Earth Sciences, Department
of Geology,

Utrecht University, P.O. Box 80.021 3508 TA Utrecht, the
Netherlands.
tel:++31 30 - 535054, fax: ++31 30 - 537725


52/125

From: SiSTek-at-aol.com Date: Mon, 13 Feb 1995 09:29:32 -0500
Subject: Need TEM analysis Contents Retrieved fromMicroscopy Listserver Archives

Help please!! SiSTek is looking for TEM analytical services

in the
southwestern US, preferably in the Phoenix metropolitan area where weare
located. We are a company that provides consultant services to a numberof
manufacturers of Si-related deposition systems and need *local*(turnaround
time and iterative analysis is important for our clients) TEMsupport. We
believe there is a company in the Phoenix area offering TEMservices, but
can't find the name. Does anyone know thename/contact?

Many thanks,

Mark Anderson, SiSTek


53/125

From: ckblack-at-dow.com(U096585) Date: Tue, 14 Feb 1995 11:03:19 -0500
Subject: mycoplasm incell culture ContentsRetrieved from Microscopy Listserver Archives

Hello folks.....

This may not be the correct forum

for the
following query, however, any info out there would
be greatlyappreciated.

We are interested in microscopy techniques,
testingprocedures, staining , etc., for detection
of mycoplasm in cellcultures.

Thankyou in advance.

Cary Black (ckblack-at-dow.com)
Dave Williams

The Dow Chemical Company


54/125

From: Doug Davis: doug_davis-at-maillink.berkeley.edu Date: 14Feb 1995 09:05:10 -0800
Subject: Ecomet Polisher for sale Contents Retrieved fromMicroscopy Listserver Archives

Message-ID: {n1419369693.44281-at-maillink.berkeley.edu}

Subject: Time: 9:13AM
OFFICE MEMO Ecomet Polisher for sale Date:2/14/95

FOR SALE: ECOMET 1 8" Polisher/grinder, complete with variouspolishing
discs, alumina and lapping oil. 115 volt, 5 amp, new price in 1988=
$1250.00
Best offer.
Call Doug Davis of EM Lab at UC Berkeley at(510) 642-2085
or e-mail: doug_davis-at-maillink.berkeley.edu


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From: jad1-at-cec.wustl.edu(Joe DeMaro) Date: Tue, 14 Feb 1995 12:02:43 -0600
Subject: SEM wellplates Contents Retrievedfrom Microscopy Listserver Archives

Any tips on cutting wells out of 24 well cell culture

plates would be
greatly appreciated.

Joe
Joseph A.DeMaro
Washington University Medical School
Department of Neurology
660S. Euclid
Rm 212 Biotech
St. Louis, MO 63110
jad1-at-cec.wustl.edu
314-362-9448


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From: Self: SALES/GREGB Date: Thu, 9 Feb 1995 13:25:54
Subject: Re: printers for gray scale prints Contents Retrieved from Microscopy ListserverArchives

Forwarded message:

All,

LaserMasterCorporation - Imaging Division is the ONLY company
that manufactures a 1800 dpiplain paper laser printers. I work for
LaserMaster and have just completedprinting the 100/300 dpi Round
Robin Greyscale Test images. If you areinterested in obtaining
them, you may e:mail me at Gregb-at-Sales.LMT.com and Iwill mail you a
printout of the test images from the LaserMaster printer. I canbe
reached at 1-800-950-6363 Ext: 3207 if you have questions aboutyour
specific situation and resolution needs.

Thank You all,
GregBegin - LaserMaster Corp.
Scientific/Medical Imaging Div.
/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\/\\/\/\/\/\/\/\/\/\/

} Date sent: Thu, 09 Feb 95 07:42:30 -600
} From:Supratik_Guha-at-mail.mmmg.com (SG)
} To: microscopy-at-aaem.amc.anl.gov
} Subject: printers for gray scale prints

} I

am looking around for a gray scale printer to attach with our Gatan
} slowscan CCD image aquisition system and would appreciate any
} suggestions. Wewould prefer not to get a dye-sub printer due to the
} high costs ofprinting. I understand that there are 1800 dpi laser
} printers available,could someone point out manufacturers for these
} machines?
}
}Supratik Guha
} Senior Materials Scientist
} 3M Corporate ResearchLabs.
}


57/125

From: NANCY SMITH: NSMITH-at-darwin.sci.csuhayward.edu Date:Tue, 14 Feb 1995 13:51:37 PSD8PDT
Subject: sem culture well Contents Retrieved fromMicroscopy Listserver Archives

Hello Joe:

When I want to process cells for SEM from24 well culture dishes I
use a Bunsen burner and a scoopula (the curved metaldevice for
scooping out dry chemicals). First, the cells are fixed as usualwith
glutaraldehyde then washed in buffer and distilled water. Then,
working within a fume hood and wearing a heatproof glove, the scoopula
isheated until red then touched to the underside of the culture dish.
The curvedmetal is about the right size for the 24 well dishes. It
takes 2 or 3 times toheat and cut until the whole bottom is
released. Once the initial cut is madeyou need to keep the cells wet
and this is easily done using a squirt bottleof distilled water.
This technique does not appear to cause damage to thecells but we
normally look at the more centrally positioned cells to avoid any
artefacts. Hope this helps.

Nancy Smith
Cal State Hayward
nsmith-at-csuhayward.edu


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From: Eric Wang: ewang-at-u.washington.edu Date: Tue, 14 Feb1995 15:07:16 -0800 (PST)
Subject: Electron mean free path Contents Retrieved fromMicroscopy Listserver Archives

X-Sender: ewang-at-hardy.u.washington.edu

Hello,everyone:
Does anyone know where I can find some reference on measurement
of electron mean free path of different materials? The mean free path I
mean here is the mean free path for measuring the thickness when doing
EELS, so this includes electrons of all the energy losses rather than one
particular energy loss. We are particularly interested in getting the
right electron mean free path for Chrome Oxide and evaporated Carbon.

Thanks a lot.

Eric Wang
FB-10 Roberts Hall
Univ. ofWashington
Seattle. Wa 98195
(206) 543-1514


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From: richard.easingwood-at-stonebow.otago.ac.nz (Richard Easingwood) Date: Wed, 15 Feb 1995 16:49:15+1100
Subject: SEM well plates Contents Retrieved from Microscopy ListserverArchives

Message-Id: {199502150442.RAA12340-at-arwen.otago.ac.nz}
Mime-Version: 1.0

)Joe,
} Subject: SEM wellplates

} Any tips on cutting wells out of 24 well cell culture plates wouldbe greatly
} } appreciated.
} Joe
} Joseph A. DeMaro

Depending onwhat exactly it is you are doing, how about using Thermanox
(Thermonox?)plastic slides on the bottom of the wells - they make them
specially to fitinto the wells of 12 well plates and possibly the 24 well
ones too. They comesterilised and you just pop them into the well before
you add medium and cellsand remove later, fix, dry etc and mount on a
stub. The slide surfaceproperties are supposed to duplicate the ordinary
well bottoms.
Its easierthan cutting wells out of the bottom of the trays.
Regards REasingwood

Richard Easingwood
South Campus Electron Microscope

Unit
Otago Medical School
PO Box 913
Dunedin
NEWZEALAND

Telephone: 64-03-479 7301
Facsimile: 64-03-479 7254


60/125

From: MatlsMicrs-at-aol.com Date: Wed, 15 Feb 1995 01:11:08 -0500
Subject: Subscribe Contents Retrieved fromMicroscopy Listserver Archives

Please Subscribe


61/125

From: Rich2442-at-aol.com Date: Wed, 15 Feb 1995 02:45:32 -0500
Subject: Request to join mailinglist Contents Retrievedfrom Microscopy Listserver Archives

I work for an OEM of scanning electron microscopes and am

interested in
keeping up with the latest technology and issues. I would liketo subscribe
to the mailing list. Could you please let me know what I need todo.


62/125

From: W.L. Steffens: STEFFENS.B-at-calc.vet.uga.edu Date: Wed, 15Feb 1995 08:41:37 EST
Subject: Cell culture plates Contents Retrieved from Microscopy ListserverArchives

We process a considerable number of cell cultures for bothSEM and TEM,
and have found what we consider to be the ideal system. For thispurpose,
we have our investigators culture their cells in Leightontubes...these
are cell culture tubes with a flat bottom which holds a long,narrow
plastic coverslip. Once the cells are attached, the medium is replaced
with fixative, then the coverslip (which is attached to a nifty little
handle) is removed. The plastic on the coverslip is impervious to all
solvents used in microscopy, and can be embedded and sectioned forTEM.

I wouldn't think of doing it any other way.

-=W.L.Steffens=-
College of Veterinary Medicine
University of Georgia


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From: Fermin, Cesar: Fermin.Cesar-at-tmc.tulane.edu Date: 15 Feb1995 09:37:10 -0600
Subject: Beseler Enlarger tune-up Contents Retrieved from Microscopy ListserverArchives

ref.: Beseler enlarger tune-up.

We have two Beselerenlarger needing adjustments. Any information
on available service person inthe south, please remit details
directly to me. Number I called wasdisconnected!

************************************************************
*Cesar D. Fermin, Ph.D \|*|/ Fax (504) 587-7389 *
*Tulane MedicalSchool /|*|\ Answ. Mach.(504) 584-2618 *
*Pathology/SL79 \|*|/Secretary (504) 584-2436 *
*New Orleans, La 70 112 /|*|\ Lab (504) 5841*
*Fermin-at-TMC.Tulane.edu -} Director of Morphological Services*
************************************************************


64/125

From: dhoyle-at-tic.ab.ca(David Hoyle) Date: Wed, 15 Feb 1995 10:59:42 -0700
Subject:registration ContentsRetrieved from Microscopy Listserver Archives
Message-Id: {25021509453900-at-vms2.macc.wisc.edu}

Thank you for responding so quickly to my inquiry.
I look forward toyour news letters and hope to be able to
contribute any information Ican.

Subscribe Microscopy dhoyle.tic.ab.ca


65/125

From: Michael Cammer: cammer-at-aecom.yu.edu Date: Wed, 15 Feb1995 11:24:52 -0500 (EST)
Subject: Re: Beseler Enlarger tune-up Contents Retrieved fromMicroscopy Listserver Archives

Back around '82 or '83 I found that I had a problem with myBesseler's
constantly slipping focus just a tiny bit. So I put pipe clamps(those
metal rings that can be tightened of loosened with the turn a a screw)on
the metal guides for the focus which I would tighten when focusingthe
bellows particulalry tight.
-mc

On 15 Feb 1995, Fermin, Cesarwrote:
} ref.: Beseler enlarger tune-up.


66/125

From: W.L. Steffens: STEFFENS.B-at-calc.vet.uga.edu Date: Wed, 15Feb 1995 13:37:13 EST
Subject: Leighton Tubes Contents Retrieved from Microscopy ListserverArchives

For those interested, Leighton tubes for cell culture aremanufactured by
Corning Costar and are available from Fisher Scientific (Cat.#07-200-
367). They appear in the 95-96 catalog on page 721.

-=W.L.Steffens=-
College of Veterinary Medicine
University of Georgia


67/125

Subject: Re:Electron mean free path Contents Retrieved from Microscopy Listserver Archives

Dear Eric,
If no expert in the field has a table of

the mfp's, I can fax you
tables of stopping powers for electrons in carbon,oxygen, iron & titanium--
you have to interpolate for chromium and calculatethe mpf from dE/dx. Good
luck.
Yours,

Bill Tivol


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From: BARBARA.HARTMAN-at-1773.220.SCHERING-PLOUGH.sprint.com Date: Thu, 16 Feb 1995 08:42:49 -0500
Subject: FIXATION OF TESTES FOR HISTOLOGY Contents Retrieved from Microscopy ListserverArchives

GREETINGS,

DOES ANYONE KNOW OF ABETTER FIXATIVE FOR TESTES THAN BOUINS FOR
LIGHT MICROSCOPY? WE ARE TRYING TOAVOID THE LONG RINSING REQUIRED WITH
BOUINS.

THANK YOU!

BARBARAHARTMAN
SCHERING-PLOUGH RESEARCH
LAFAYETTE, NJ
(201) 579-4343
(201)570-4211 (FAX)

E-MAIL:
MAIL/ADMD=TELEMAIL/PRMD=SCHERING-PLOUGH/PN=BARBARA.HARTMAN/C=US/-at-SPRINT.COM


69/125

From: SiSTek-at-aol.com Date: Thu, 16 Feb 1995 13:48:59 -0500
Subject: Need TEM / Many thanks!! Contents Retrieved fromMicroscopy Listserver Archives

Many thanks to all who responded for my call for help with

locating TEM
service near us in the Phoenix metro area. As we thought, there isan
established group in Phoenix who have been around for a few years andwho
provide TEM services.

For anyone else who might be interested, thecompany is called NanoTEM, Inc,
7620 E. McKellips Rd., Suite 4109, Scottsdale,Arizona 85257, phone 602 759
2808, fax 602 947 7615.

Again, manythanks for all your help.

Mark Anderson, SiSTek


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From: baskin-at-biosci.mbp.missouri.edu (Tobias Baskin) Date: Fri, 17 Feb 1995 13:47:11 -0600
Subject: TEM/formvar substitute/thin films Contents Retrieved from Microscopy ListserverArchives

Greetings,
Is there something out there thatwill make a thin film that
isn't formvar? I have been using wire loops coatedwith a film of 1.2%
formvar to support my small samples during rapid freezingand substitution.
This works fine for acetone substitution but we would like totry
Tetrahydrafuran (THF) as a substitution medium and, alas, THF eatsthe
formvar.

We get a formvar film on the wire loop by castingsmall rectangles
of formvar on water and then trasfering one to a loop.

Are there other compounds that could be used to make a film across
the loop andthat might survive THF??

Thanks for any suggestions,

Tobias Baskin

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - -
___ ____ ^ ____ _____ Tobias I.Baskin
/ \ / / \ / \ / University ofMissouri
/ | / / \ / / Biological

Sciences
/___ / /__ /_____\ / /__ 109 Tucker Hall
/ / / \ ( / Columbia, MO 65211 USA
// / \ \ / voice: 314-882-0173
/ /____ /\ \____/ /_____ fax: 314-882-0123


71/125

From: Liang, Long: LLIANG-at-is.Arco.COM Date: 17 Feb 199514:34:14 CST
Subject: Sample Prep--Steel Contents Retrieved from Microscopy ListserverArchives

Message-Id: {MACMS.LLIANG.644426140095048FMACMS-at-IS.ARCO.COM}

Dear Microscopists,

I am trying to preparepolished sections from steel samples for EPMA
analysis. Are there anyrecommended abrasive/size for rough grinding,
fine grinding, rough polishing,and final polishing?

Your help is high appreciated.

LongLiang
ARCO EPMA/SEM Lab
Plano, TX
LLIANG-at-is.arco.com


72/125

From: Doug Arrell: ARRELL-at-jrc.nl Date: Mon, 20 Feb 199508:25:04 GMT+0200
Subject: Re: Sample Prep--Steel Contents Retrieved from Microscopy ListserverArchives

Message-Id: {MAILQUEUE-101.950220082504.256-at-FS-IAM-1.JRC.NL}

}
} I am trying to prepare polished sections from steelsamples for EPMA
} analysis. Are there any recommended abrasive/size forrough grinding,
} fine grinding, rough polishing, and finalpolishing?

I have always stuck to the simple silicon carbide paper (insteps
from 120 to 1200 grade) and then diamond (6,3,1um) route, and found
no problems withthat.

Doug

+------------------------------------+
| Dr DouglasArrell |
| Mechanical Performance and Joining |
| Institutefor Advanced Materials |
| 1755 ZG Petten |
|Netherlands |
| {ARRELL-at-JRC.NL}|
| Tel. (+31) 2246 5287 |
| Fax (+31) 2246 1917|
+------------------------------------+


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From: Joyce Craig: bafpjec-at-uxa.ecn.bgu.edu Date: Mon, 20 Feb1995 11:10:51 -0600 (CST)
Subject: student microscopy competition Contents Retrieved fromMicroscopy Listserver Archives

CALL FOR PAPERS
STUDENT COMPETITION
TO BE HELDAT
THE UNIVERSITY OF WISCONSIN
WHITEWATER, WISCONSIN
Friday, March 24,1994
AWARDS:
FIRST PLACE $100
SECOND PLACE $75
THIRD PLACE$25
Abstracts will be published in Midwest Microscopy.
Microsgraphs fromfirst place winner will be on the cover of Midwest
Microscopy.
Studentswill be judged on written abstract, presentation, and quality of
study.
Student competition is open to undergraduate and graduate students and
mayinvolve any type of microscopy.
S

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