Real-time PCR automated solution for food pathogen testingJean-Philippe Tourniaire – International Product Manager

Lab Innovations, Birmingham, November 6th 2013

iQ-Check Prep Solution goals

• Straightened our global offer on iQ-Check Food pathogen testing

• Provide technically advanced solutions to medium to high throughput laboratories

• Provide confidence and peace of mind with a cost effective solution

iQ-Check Prep Solution Concept

• Complete automation of the iQ-Check Easy protocols

• From the enriched sample to the PCR plate ready

• Two plates handled at the same time

• Run of up to 4 different parameters on a plate

• Compatible with all the iQ-Check Food kits

• Optimal integrated solution with user-friendly interface: CFX Manager software IDE

• Partnership with Hamilton http://www.hamiltonrobotics.com, a WW leader in automation

Automation Concept

Automated

The iQ-Check Prep System and his Deck

Deck description

DW plate for dilutions (Transfer)

1 2 3 4

5 6 7 8

50 µL tips

1000 µL tips

PCR plate loading place

DW plate 1 for samples

DW plate 2 for samples

DW plate 2 for extraction

DW plate 1 for extraction

PCR plates

1 & 2

Gripper

Agitator / incubator

Tubes for PCR mix

PCR reagents

kits

Lysis bottles

Water container

1 2 3 4

Waste

1000 µL tips

Rack 1

mobile

Rack 2

Non mobile

Rack 3

mobile

Rack 4

mobile

1 4

2 3

Technology

Air displacement pipetting principle

Tips pick-up with CO-RE Technology

Type of tip recognition

• 4 channels

Technology

Added value of the conductive tips:

Liquid level sensing (aspiration / dispensing) for increased safety and confidence

Monitored Air Displacement: MAD

Liquid Level Detection: LDD

iQ-Check Prep User Interface

Two computers and software needed:

1. CFX ManagerTM Software IDE v2.0 : handles the CFX96 Deep WellTM Touch (and MiniOpticonTM) and allows the plate set-up for the iQ-Check Prep

CFX Manager Software IDE v2.0

iQ-Check Prep Software - User Interface

2. iQ-Check Prep Software v1.3: gets information from the CFX Manager IDE v2.0 and handles the protocols on the iQ-Check Prep instrument. The user is guided through the different steps to start the run : refill of consumables and reagents

Throughput Capacities / Workflow

• Highest throughput configuration:

•2 x 94 samples / prep run : about 2 x 70 min •2 hrs PCR•Process 6 plates in 8 hrs

• Time duration of different protocols or steps•Complete run : 1h10 min•DNA already extracted: 30 min•1/10 dilution: + 15 min•Lysis reagent dispensing: 8 min•PCR mix preparation and dispensing: 19 min•Cooling the Deep Well plate (DNA extract): 25 min

Throughput Capacities / Workflow

Hr

Sample transfer 1iQ-Check Prep 1

PCR 1 96

Sample transfer 2iQ-Check Prep 2

PCR 2 192

Sample transfer 3iQ-Check Prep 3

PCR 3 288

Sample transfer 4iQ-Check Prep 4

PCR 4 384

Sample transfer 5iQ-Check Prep 5

PCR 5 480

Sample transfer 6iQ-Check Prep 6

PCR 6 576

6 7 80 1 2 3 4 5

Hr

Sample transfer 1iQ-Check Prep 1

PCR 1 96

Sample transfer 2iQ-Check Prep 2

PCR 2 192

Sample transfer 3iQ-Check Prep 3

PCR 3 288

6 7 80 1 2 3 4 5Configuration 1:

1 iQ-Check Prep + 1 CFX96 Deep Well Touch IDE

More than 250 samples tested in 8 hrs

Configuration 2:

1 iQ-Check Prep + 2 CFX96 Deep Well Touch IDE

More than 500 samples tested in 8 hrs

Absence of Cross Contamination

One of the major concern for this project: tested many times with highly positive and negative samples

Perfectly clean results and deck!!!

AFNOR and AOAC-RI Validations

Features and Benefits

Benefit Feature

• Complete transparency and possibility to handle manually the samples if needed

• Manual protocol automatized without modification

• Recognized solution with validated performances

• AFNOR and AOAC-RI validated solutions

• High confidence and traceability • Handles complete DNA extraction and

PCR plate preparation

• Improved confidence in liquid handling• Conductive tips, pipetting control with

liquid level sensing

Features and Benefits

Benefit Feature

• Avoid cross contamination risks with aerosols and droplets

• CORE technology

• Limited maintenance and wastes: no tubing, pump, liquids,….

• Air displacement technology

• Faster than any other automation system, ideal for high throughput labs

• Process 96 tests in a little more than 3 hours (more than 250 or 500 samples / 8 hours)

• Easy to learn and to use• Optimized traceability between the iQ-

Check Prep and the CFX96 Touch Deep Well real-time PCR instrument

• Handled through the use of the CFX Manager IDE and the iQ-Check Prep software

Features and Benefits

Benefit Feature

• Optimized flexibility to handle all major parameters

• Compatible with all iQ-Check Food kits and up to 4 parameters / plate

• Save time : no need to dissolve, rehydrate, defreeze,….

• Ready to use reagents

• No disruption of lab workflow: reallocate your lab resources

• Proceed with samples by batch

• Save time in handling and managing your results

• LIMS connection, email results notification

• Save time for support and anticipate any maintenance action

• Remote control to monitor log files for preventive maintenance and support

Key Benefits

• Easy to use

• LIMS connection to import & export data

• Improved throughput capacities

• Improved traceability

• Increased accuracy: less handling errors

• Save technician handling time

• Simple transposition of the manual protocol (not a black

box)

• Pipetting control

• Limited maintenance (air displacement)

• AFNOR and AOAC validated

Feedback From Users

J. P., evaluator• “we really like the Prep station …. The advantages are that it virtually

eliminates the potential for liquid handling and cross contamination errors. The operation of the Prep station is straightforward and the software is easily navigable providing the operator with prompts, diagrams and help/troubleshooting screens. The PCR detection software is also user-friendly and intuitive….”

C.S., evaluator• Easy to use• Great and improved PCR results• Save minimum 1 hr technician time / day

From competitor• “It’s hard to make it simple, and you did it!”

iQ-Check Prep Solution

Thanks a lot for your attention!