reametrix hiv reagent portfolio
DESCRIPTION
ReaMetrix HIV ReagentsTRANSCRIPT
© 2008 Reametrix, Inc. All rights reserved.
Management of HIV Infection in Resource Poor Settings
Accurate & Affordable Reagent Alternatives
2© 2008 Reametrix, Inc. All rights reserved.
HIV in India – Current Status
HIV Prevalence rate in India 0.36% 2.5 Million HIV Infections
By year 2010, estimates indicate that there will be between 8-15 million HIV infected individuals
Management of HIV InfectionProviding Anti Retroviral therapy (ART)Immunophenotyping
CD4 T-Lymphocyte Count
Viral Load Assay – PCR – quantitate virus levels in blood
CD4 counts are the single most important criteria to guide clinical decision-making on
when to use ART
3© 2008 Reametrix, Inc. All rights reserved.
ReaMetrix Solutions for CD4 EnumerationDry-Tri T-STAT CD3483 color single tube reagent for open flow Cytometers eg. BD FACScan ™, BD FACSCalibur ™, BC EPICS XL™)
Dry-Tri T-STAT CD34453 color single tube reagent for open flow Cytometers(eg. BD FACScan™, BD FACSCalibur™, BC EPICS XL™)
ReaPan 348454 color single tube reagent for open Flow Cytometers(eg. BD FACSCalibur™)
Rea T count2 color dual tube reagent for FACScount™ Platform
ReaPan 34G-38G2 Color dual tube reagent for the Guava™ Platform
4© 2008 Reametrix, Inc. All rights reserved.
ReaMetrix Assay Reagent Characteristics Methodology
Absolute CD4 testing by flow Cytometry – gold standard method
Employ simple Lyse/no wash protocol - single platform technology
Convenience Provided as a ready to use format. No additional dispensing
Unitized formPre-dispensed reagents in unitized form – reduces pipetting
errors
Stability & storageDried down reagents – do not require cold storage. No
aggregation of antibodies & beads in dried form
Simplified work flowMinimal hand on time - just require sample pipetting, reduced
steps
5© 2008 Reametrix, Inc. All rights reserved.
ReaMetrix Assay Reagent Characteristics
Accurate & reproducibleWorkshop approved antibody clones – provide excellent
Analytical Specificity & Analytical Sensitivity Extensively validated reagents in various private & public labs
in India
Sample stabilityProcessed samples can be stored for up to 7 days prior to analysis (CV = 5.7%).
AffordableAll of these attributes without compromising affordability
Technical supportReaMetrix provides training on Cytometer and reagentReaMetrix provides technical support online & through personal
visits
6© 2008 Reametrix, Inc. All rights reserved.
Reagents are suitable to run on open flow cytometers such as BD FACScan, BD FACSCalibur, Beckman Coulter EPICS-XL etc.
Reagents supplied in Dry, Unitized Format
Dry-Tri T-STAT CD348
3 color single tube reagentAnti CD4 – Atto488* (FITC eq. dye)Anti CD8 – R-PEAnti CD3 – R-PE-Dy649* (Cy5 eq. dye)
Provides absolute counts (Cells/µL) of CD3+, CD4+, CD8+ Cells & CD4/CD8 Ratio Validated with commercial reagents at NARI, YRG CareCE Marked reagent
* Dyes qualified by ReaMetrix to assure superior performance
7© 2008 Reametrix, Inc. All rights reserved.
Reagents are suitable to run on open flow cytometers such as BD FACScan, BD FACSCalibur, Beckman Coulter EPICS-XL etc.
Reagents supplied in Dry, Unitized Format
Dry-Tri T-STAT CD3445
3 color single tube reagentAnti CD4 – Atto488* (FITC eq. dye)Anti CD3 – R-PE-Dy649* (Cy5 eq. dye)Anti CD45 – R-PE
Provides absolute counts (Cells/µL) of Absolute Lymphocyte Count (ALC), CD3+, CD4+, % CD4 CountsValidated with commercial reagents at leading path labs in IndiaSuited for aged blood samples
* Dyes qualified by ReaMetrix to assure superior performance
8© 2008 Reametrix, Inc. All rights reserved.
Reagents supplied in Dry, Unitized Format
ReaPan 34845
44 color single tube reagent, Anti CD4 – APC Anti CD8 – R-PEAnti CD3 – Atto488* (FITC eq. dye)Anti CD45 – PerCP
Provides absolute counts (Cells/µL) of ALC, CD3+, CD4+, CD8+ Cells, % CD4 & CD4/CD8 Ratio Validated with BD multiTEST 4 color reagent at leading path. Lab in India.Suited for aged blood samplesDesigned for BD FACSCalibur dual laser flow CytometerCE marked * Dyes qualified by ReaMetrix to assure superior
performance
9© 2008 Reametrix, Inc. All rights reserved.
Reagents supplied in Dry, Unitized Format
Rea T-Count Reagent
Rea T-count CD3/CD4 & CD3/CD8 reagentagentDesigned for BD FACSCount flow CytometerAvailable as 2 color dual tube reagent for
CD3+, CD4+, CD8+, CD4/CD8 ratio estimation
Available as 2 color single tube reagent for
CD3+, CD4+ estimation
Alternative for FACSCount reagent Reagent validated at
National HIV Immunology laboratory, CanadaNational HIV Immunology laboratory, Canada
ZimbabweZimbabwe
10© 2008 Reametrix, Inc. All rights reserved.
Reagents supplied in dry, unitized format
ReaPan Reagents for the Guava Platform
ReaPan 34G2 color single tube reagent Provides absolute counts (Cells/µL) of CD3+, CD3+CD4+ counts
ReaPan 34G & 38G2 color single dual reagentProvides absolute counts (Cells/µL) of CD3+, CD3+CD4+ & CD3+CD8+ counts
Validated with Guava Reagent at KIMS, Bangalore
11© 2008 Reametrix, Inc. All rights reserved.
Summary…
ReaMetrix supplies all reagents with ReaLyse, a buffer for lysing and fixing samples or ReaFix, a buffer for fixing samples after staining
ReaMetrix has the capability to develop CD4+ enumeration solutions for any flow cytometer platform.
ReaMetrix can customize solutions as per your need.
Work is in progress for reagent development for other platforms.
12© 2008 Reametrix, Inc. All rights reserved.
Manufactured by ReaMetrix India Pvt. Ltd.50-B, II Phase, Peenya Industrial Area
Peenya, Bangalore 560058, IndiaPh: +91-80-28378693/5, Fax: +91-80-
41172451E-mail: [email protected]
www.reametrix.com
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