Copyright © 1999, W.B. Saunders Company

A general Method for constructing recombinant vaccinia viruses

Ref: Roche mRNA isolation kit

Purification of mRNA

Restriction & Ligation

Insertion of Restriction Fragment into Vector

Multi Cloning Site

The pUC 18 or 19 Cloning Vector

Lac Z α

Transformation and Selection

Screening

White White Blue Dead

Types of ELISA

Ref: Kuby Immunology 7th edition

NORTHERN BLOTTING

Ref: Wikimedia commons