repair of chondral defects and meniscus using … · the project medcel (pse-010000-2007-4) was...
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REPAIR OF CHONDRAL DEFECTS AND MENISCUSUSING AUTOLOGOUS MESENCHYMAL STEM CELLS
A PRELIMINARY STUDY IN SHEEP
J. Vives, F. García-Arnás, J. Barrachina, J. García, R. Soler-Rich, L. [email protected]
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Methods and MaterialsTen 2-year old ewes were divided in two groups(for analysis at 6 and 12 month, respectively). Arthroscopically guided chondral lesions of approximately 60 mm2 were generated in thefemoral medial condyles and piercing theanterior horn of the medial meniscus of thetwo posterior extremities. Cellular treatmentswere applied at a maximum dosage of 50x106
MSCs 30 days later and the animals weremonitored by MRI, ecography and X ray. Potential adverse effects of the treatment wereevaluated by a full necropsy performed at 12 months. This study was approved by the EthicsCommittee on Animal and Human Research of the Universitat Autònoma de Barcelona and registered by the Departament de MediAmbient from the Generalitat de Catalunya, registration No. 4179 (ref. CEAAH 688).
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In this work, autologous mesenchymal cells (MSC) expanded from bone marrow aspirates proved safe and induced regeneration of both hyaline cartilage and meniscal fibrocartilagewith the advantage of using a straightforward infiltration approach. Analysis of experimental animals after 6 and 12 months post-treatment revealed a significant degree of regeneration in those condyles where MSC were infiltrated. The hyaline quality of the newly formed cartilage was further confirmed by histological analysis. Interestingly, meniscallesions were also partially repaired. A general improvement was observed at 12 month compared to 6 month post-treatment independetnly of treatment. Most importantly, the intra-joint application of autologous MSC was demonstrated to be safe by a full necropsy performed at 12 months post-treatment.
Analysis of condyles at 12 months post-treatment.Treated medial condyles presented a reduction of the extent of lesioned cartilage compared to controls. The depth was significantly regenerated and integrated withadjacent normal cartilage. The aspect of the new tissue is similar to normal both at the macroscopic and histological levels.
Analysis of meniscus at 12 months post-treatment. The medial meniscus in sheep treated withoMSC presented macroscopic, ecographical and MRI properties compatible with healthy meniscus. In contrast, treatment with PRP without oMSC didn’t contribute to the regeneration of meniscus
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The project MEDCEL (PSE-010000-2007-4) was supported by the Spanish Ministry of Education and Science (MEC)
Analysis at 12 monthsRx, MRI, Eco, Full necropsy, Histology
Analysis at 6 monthsRx, MRI, Eco, Histology
TreatmentEcoguided infiltration of autologous MSC expanded from bone marrow
ModellingGenerate lesionarthroscopically
9th World Congress of the International Cartilage Repair Society - 2010