1

2

For the director of

CTBE, Carlos Alberto

/DEDWH��WKH�VLJQL´-

cant results achieved

by the Laboratory in

2013 are due to the

successful combina-

tion of professionals

with experience in

bioenergy and young

researchers willing to

produce it.

3

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Another project moving

forward was the low impact me-

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Prototypes of the base machine

and harvest heads of the Control-

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their test phase, in order to incre-

ase productivity and reduce soil

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6WXGLHV� RI� SUHFLVLRQ� DJULFXOWXUH�

FRPSOHPHQW�WKH�SURMHFW�

In 2013 it was also started new

projects in partnership with ma-

jor companies in the chemical and

sugar-energy sector, many of them

VXSSRUWHG� E\� %1'(6� DQG� )LQHS��

This is a tendency to be expanded

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FRQQHFW�&7%(�WR�SULYDWH�VHFWRU�DQG�

large research institutes in Brazil

RQ�ELRPDVV�DQG�ELRHQHUJ\�´HOG��LQ�

RUGHU� WR�VROYH�VFLHQWL´F�DQG� WHFK-

nological bottlenecks.

Carlos Alberto Labate

Director of CTBE

7KH�VXFFHVV�RI�&7%(� LV� ODUJH-

ly due to a perfect combination

RI� H[SHULHQFH� DQG� \RXQJ� WDOHQWV��

The laboratory has an excellent

infrastructure for scale up of etha-

nol production technologies and

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It also count on with experienced

professionals at bioenergy direc-

ting the ability of young researchers

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rential when facing complex and

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0\�DUULYDO�DW�&7%(�� LQ�'HFHPEHU�

2012, occurred at an important

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a transition from implantation to

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SURMHFWV�VWDUWHG�WR�DFKLHYH�LWV�´UVW�

VLJQL´FDQW�UHVXOWV�

It was the case, for example, of

the Pilot Plant for Process Develop-

PHQW� �33'3��� )RU� WKH� ´UVW� WLPH�� D�

biotechnological process was vali-

dated in the PPDP for seven con-

secutive months, 24 hours a day,

VHYHQ� GD\V� D� ZHHN�� 7KLV� VKRZHG�

the proper commissioning of the

facility and its suitability for large

scheduling initiatives in the ethanol

message ofthe director

4

Aerial view of CNPEM campus in Campinas-SP, with architectural project of the new

synchrotron light source (Sirius), under construction.

5

DERXW�&7%(� � � � S����

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contents

CNPEM

The Brazilian Center for Research in Energy and Materials (CNPEM), located LQ�WKH�FLW\�RI�&DPSLQDV� �63��LV�D�SULYDWH�QRQSUR´W�RUJDQL]DWLRQ�TXDOL HG�E\�WKH�Ministry of Science, Technology and Innovation (MCTI) to manage four national laboratories: Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Biosciences National Laboratory (LNBio), Brazilian Bioethanol Science and Technology La-boratory (CTBE) and Brazilian Nanotechnology National Laboratory (LNNano).

LNLS operates the only Synchrotron Light Source in Latin America, LNBio conducts research focused on biotechnology and pharmaceuticals, CTBE inves-tigates new technologies for bioenergy production and LNNano conducts rese-arch in advanced materials, with high economic potential for Brazil.

These laboratories develop their own research projects and participate of WKH�WUDQVYHUVDO�DJHQGD�FRRUGLQDWHG�E\�&13(0��FRPELQLQJ�IDFLOLWLHV�DQG�VFLHQWL F�skills around strategic themes.

6

Facade of the CTBE

main building, Lab

created with the aim

of contributing to the

Brazilian competi-

tiveness in industrial

production and con-

version of biomass

into fuels and other

high added-value

compounds.

7

technical, economic, environmental

DQG� VRFLDO� DVVHVVPHQW� RI� ELRUH´-

nery systems is also available to the

VXJDU�HQHUJ\�LQGXVWU\�

The Pilot Plant for Process De-

YHORSPHQW� �33'3�� LV� D� PDMRU� GL-

IIHUHQWLDO�RI� WKH�&7%(��7KLV�XQLTXH�

environment in Brazil allows the

VFDOH�XS�RI�SURFHVVHV��VHPL�LQGXV-

WULDO� VFDOH� ¨� ���� OLWHUV�� UHODWHG� WR�

biomass conversion into cellulosic

ethanol and other products pre-

VHQW�LQ�WKH�WKH�ELREDVHG�HFRQRP\�

One the other hand the

Laboratory’s internal research

agenda is dedicated to agricultu-

ral, industrial, technological assess-

ment, sustainability and basic rese-

arch of biomass/bioproducts areas,

LQ�WXQH�ZLWK�WKH�PDUNHW�QHHGV�

Vision

Being a National Laboratory

of excellence in research, develo-

pment, innovation and technology

transfer in the biomass and bioe-

QHUJ\�DUHDV�

7KH�%UD]LOLDQ�%LRHWKDQRO�6FLHQ-

ce and Technology Laboratory

�&7%(�� FRQGXFWV� 5'� RI� H[FHO-

OHQFH� LQ� WKH� ELRHQHUJ\� ´HOG�� 7KH�

main goal of the Laboratory is to

contribute to the Brazilian com-

petitiveness in production and in-

dustrial conversion of biomass into

IXHOV��´UVW�DQG�VHFRQG�JHQHUDWLRQ���

electricity and compounds derived

IURP�JUHHQ�FKHPLVWU\�

,Q� &7%(�� VRSKLVWLFDWHG� IDFLOL-

WLHV�� LQVWUXPHQWDWLRQ� DQG� VFLHQWL´F�

and technical support are offered

for Brazilian and foreign users that

work with production, characteri-

zation and processing of biomass

and derivatives as well as studies

of functional biology, biotechnolo-

J\� DQG� ELRSK\VLFV�� $� SODWIRUP� IRU�

Mission

Contribute to the advance-

PHQW�RI�VFLHQWL´F�DQG�WHFKQRORJL-

cal knowledge in the production,

conversion and use of biomass for

energy and materials, through re-

search, development, innovation

DQG�SURIHVVLRQDO�WUDLQLQJ�

about CTBE

8

High Quality Human

Capital and Publications

Patents and Win-win

Partnerships with

Producive Sector

Sophisticated

R&D Environment

Research in the Whole

Productive Cycle of

Biomass/bioproducts

Scale up of Bioenergy

Process

Events: Networking

and Dissemination

of Results

Relevant Projects: Low Impact Mechanization

DQG�9LUWXDO�6XJDUFDQH�%LRUH �QHU\

9

the sugarcane crop, with the deve-

lopment of machinery that may re-

GXFH�WUDI´F�E\�����RYHU�WKH�´HOGV��

promoting increases in sugarcane

yield and lifespan, reducing dama-

JHV�LQ�VWRROV�DQG�VDYLQJ�IXHO�

• It holds a platform for te-

chnical, economic, environmental

DQG� VRFLDO� DVVHVVPHQW� RI� ELRUH´-

QHU\�V\VWHPV�FDOOHG�9LUWXDO�6XJDU-

FDQH�%LRUH´QHU\�

• It ended 2013 with 215 pro-

IHVVLRQDOV�� EHLQJ� ��� UHVHDUFKHUV�

DQG����VFLHQWL´F�DGYLVRUV�RI�UHQRZ-

QHG�UHVHDUFK�LQVWLWXWLRQV�

• It has published over 230

VFLHQWL´F� DUWLFOHV� LQ� LQGH[HG� MRXU-

QDOV�DQG����SDWHQWV�VLQFH������

• It signed 13 cooperation

agreements with companies in

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�� � ,W� SURPRWHV� KLJK� TXDOLW\�

events in the bioenergy area, es-

pecially the annual workshops

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(Q]\PDWLF� +\GURO\VLV� DQG� 6XJDU-

cane Physiology for Agronomic

$SSOLFDWLRQV�

)LQG�RXW�WKH�PDLQ�IHDWXUHV�DQG�

RXWFRPHV�RI�WKH�&7%(�

• It undertakes research and

innovation at the international le-

vel in the area of biomass for ener-

gy production, especially ethanol

IURP�VXJDUFDQH�

�� ,W�GHYHORSV�´YH�UHVHDUFK�GL-

YLVLRQV�� %LRPDVV� 3URGXFWLRQ�� %LR-

PDVV�3URFHVVLQJ��)XQFWLRQDO�%LROR-

J\��%LRWHFKQRORJ\�DQG�%LRSK\VLFV��

,QWHJUDWHG�(YDOXDWLRQ�RI�%LRUH´QH-

ULHV�DQG��6XVWDLQDELOLW\�RI�%LRPDVV�

DQG�%LRHQHUJ\�3URGXFWLRQ�

• It houses three buildings

�������P2�RI�EXLOW�DUHD���WKH�SULQ-

cipal, which includes laborato-

ries and researchers’ rooms, Pilot

Plant for Process Development

�33'3�� DQG� $JULFXOWXUDO� 3URWR-

W\SHV�/DERUDWRU\�

�� 6WDQGV�RXW�IRU�LWV�LQIUDVWUXF-

ture designed for technology sca-

le up, with the purpose of trans-

ferring processes from laboratory

EHQFK�WR�WKH�SURGXFWLYH�VHFWRU�

• It works in the establishment

of low impact mechanization on

at a glance

10

2005

Development of the Ethanol Project

�3KDVH�����ZKRVH� �QGLQJV�LQVSLUHG�

the research divisions and projects

of the CTBE. The physicist Rogério

&��GH�&HUTXHLUD�/HLWH��81,&$03��

coordinated the study that was

attended by about 20 professionals

from different institutions.

our history

November 2008

Beginning of the construction of

CTBE facilities. Three buildings

were constructed totaling

approximately 9,000 m2, in the

Campus of CNPEM.

February 2009

First workshop was performed

for the evaluation of CTBE

research divisions. In two years,

representatives of the academic

and industrial communities

participated, at different times, in

the discussion of the themes and

UHVHDUFK�JRDOV�RI�DOO� �YH�GLYLVLRQV�from different institutions.

November 2008

Beginning of the construction of

CTBE facilities. Three buildings

were constructed totaling

approximately 9,000 m2, in the 2, in the 2

Campus of CNPEM.

UHVHDUFK�JRDOV�RI�DOO� �YH�GLYLVLRQV�

11

August 2013

&RQFOXVLRQ��IRU�WKH� �UVW�WLPH��

of a biotechnological process

scale up in the Pilot Plant that

made bioreactors and other

utilities remained in uninterrupted

operation during seven

months, certifying the proper

commissioning of the facility.

January 22, 2010

Opening of the CTBE, which

counted with the presence of

former President of Brazil, Luiz

lnácio Lula da Silva, and other

regional and national authorities.

The ceremony also celebrated

WKH�VLJQLQJ�RI�WKH� �UVW�WKUHH�

partnerships of the Laboratory.

May 2011

Signing of agreement with BNDES

(Funtec line) which provides

LQYHVWPHQW�RI�5�����PLOOLRQ�RYHU� �YH�

years for the implementation of low

impact mechanization management

on the sugarcane crop.

commissioning of the facility.partnerships of the Laboratory.

May 2011

Signing of agreement with BNDES

(Funtec line) which provides

LQYHVWPHQW�RI�5�����PLOOLRQ�RYHU� �YH�

years for the implementation of low

impact mechanization management

12

Hydrolysis reactor of

biomass in labora-

tory scale. It is used

WR�WHVW�WKH�HI´FDF\�

of enzyme cock-

tails developed for

industrial use in the

second generation

ethanol production

13

Bioprocesses for Microorganisms

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PDWLF�+\GURO\VLV�RI�/LJQRFHOOXORVLF�

0DWHULDO�� 6HSDUDWLRQ� DQG� 3XUL´FD-

WLRQ��$OFRKROLF�)HUPHQWDWLRQ�

Agricultural Prototypes

Labotatory (LPA)

There it is developed machi-

nery necessary for the implemen-

tation of low impact mechanization

IRU� VXJDUFDQH� FURS�� 7KLV� SURMHFW�

DLPV�WR�UHGXFH�WUDI´F�RI�PDFKLQHV�

RQ�WKH�´HOGV�DQG�LQFUHDVH�VRLO�TXD-

OLW\�DQG�SODQW�SURGXFWLYLW\��)RU�WKLV��

it is developed at LPA a Controlled

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WKH�´UVW�SURWRW\SHV��EDVH�PDFKLQH�

DQG�KDUYHVW�KHDGV��ZHUH�WHVWHG�LQ�

´HOGV�LQ������

Processes

Development

Laboratories (LDP)

7KH�/'3�LV�HTXLSSHG�ZLWK�PXO-

tiple reactors capable of proces-

sing lignocellulosic biomass and

its derivatives for conversion into

cellulosic ethanol, chemical buil-

GLQJ� EORFNV� DQG� RWKHU� SURGXFWV��

&7%(� KRXVHV� D� VRSKLVWLFDWHG�

infrastructure of science and te-

chnology in the biomass and bio-

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is the possibility of processes scale

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6HH� IXUWKHU� LQIRUPDWLRQ�DERXW�

Laboratories and Pilot Plant of the

&7%(�

Pilot Plant for Process

Development (PPDP)

Professionals use the Pilot

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and demonstrate, in semi-indus-

WULDO� VFDOH� ����� OLWHUV��� UREXVWQHVV�

and stability of technological rou-

tes for production of ethanol and

other compounds derived from

ELRPDVV�� ,W� HQDEOHV� WR� YDOLGDWH�

techno-economic and sustainabi-

lity indicators of processes, mini-

mizing risks, provides bottlenecks

and optimizes processes and in-

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6L[� LQGHSHQGHQW�DQG�DXWRPD-

WHG� PRGXOHV� LQWHJUDWH� WKH� 33'3��

3K\VLFDO� 7UHDWPHQW� RI� 6XJDUFDQH�

%DJDVVH�� 3K\VLFDO�&KHPLFDO� 7UH-

DWPHQW� RI� 6XJDUFDQH� %DJDVVH��

R&D facilities

14

In these Labs researchers stu-

dy and develop biotechnological

processes related to ethanol pro-

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GXVWULDO�SURGXFWV� IURP�VXJDUFDQH��

The facilities of microbial cultiva-

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pped with bioreactors from diffe-

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DQG� ��� OLWHUV�� ´WWHG� ZLWK� RQOLQH�

monitoring probes for measure of

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,W� DOVR� LQFOXGHV� DFFHVVRU\� HTXLS-

ment for monitoring bioprocesses

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cabinets, microbiological gree-

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7KH� ODEV� KDYH� WKH� FHUWL´FDWH�

RI� TXDOLW\� LQ� ELRVDIHW\�� DOORZLQJ�

conducting research and develo-

pment of process with genetically

PRGL´HG�RUJDQLVPV�LQ�ULVN�OHYHO���

Wide range of physical and chemi-

cal processes are studied in order

to validate processes and develop

new routes, seeking technology

WUDQVIHU��7KH�/'3�DOVR�KRXVHV� WKH�

Analytical Center for Biomass and

'HULYDWLYHV�RI� WKH�&7%(� WKDW�SHU-

forms, as routine, several analytical

protocols applied to biomass pro-

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The following laboratories

FRPSRVH� WKH� /'3�� )UDFWLRQDWLRQ�

DQG� 0LOOLQJ�� %LRPDVV� 3UHWUHDW-

PHQW�� 3K\VLFRFKHPLFDO� $QDO\VLV��

/LTXLG� &KURPDWRJUDSK\�� 6ROLG-

�6WDWH� $QDO\VLV�� 6SHFWURVFRS\�

DQG� (OHPHQWDO� $QDO\VLV�� 1XFOH-

DU� 0DJQHWLF� 5HVRQDQFH� DQG� *DV�

&KURPDWRJUDSK\�

Bioprocesses Development

Laboratories (LDB)

Panoramic image of the Pilot Plant for Process Development (PPDP) of the CTBE, used for

scale up of technological routes for the production of ethanol and other compounds derived

from biomass.

15

such as functional genomics,

metagenomics, transcriptomics,

proteomics, systems biology,

genetics and metabolic engine-

ering for bio-prospecting of mi-

croorganisms and enzymes and

for understanding the molecular

mechanisms involved in the plant

biomass conversion by enzymatic

V\VWHPV��0ROHFXODU�HQJLQHHULQJ�LV�

also used in order to improve the

catalytic performance of charac-

WHUL]HG�HQ]\PHV�

Integrate this cluster of labs

WKH� IROORZLQJ� IDFLOLWLHV�� 0HWDER-

ORPLFV�� 0LFURRUJDQLVP� &XOWXUH��

+LJK�7KURXJKSXW�6FUHHQLQJ��0D-

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%LRVDIHW\� /HYHO� ,,�� 3ODQW� 3K\VLR-

ORJ\��&RPSXWDWLRQDO�%LRORJ\�DQG�

(�6FLHQFH�

The following laboratories In-

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)XQJDO� +\GURODVHV�� %LRV\QWKHVLV�

RI� %DFWHULDO� +\GURODVHV�� $OFRKR-

OLF� )HUPHQWDWLRQ�� ,QVWUXPHQWDWLRQ�

DQG� 0RQLWRULQJ� RI� %LRSURFHVVHV��

,QGXVWULDO�8VHU�,�DQG�,,��$SSOLHG�5H-

VHDUFK�IRU�,QVWLWXWLRQDO�8VHU�

Functional Biology,

Biotechnology and

Biophysics Laboratories

Plant physiology studies

analyze the relationship between

photosynthesis and biomass ac-

cumulation, assessment of water

SRWHQWLDO��DPRQJ�RWKHUV��6WDQG�RXW�

LQ� WKLV� FRQWH[W�� HTXLSPHQW� VXFK�

DV�'XDO�3$0�����µXRURPHWHU�DQG�

WKH�6FKRODQGHU�W\SH�FDPHUD�

In the molecular biology area

scientists employ approaches,

16

• low impact mechanization• conservation agriculture��WHFKQLTXHV�RI�SUHFLVLRQ�DJULFXOWXUH• nutrients and agro-industrial residues management• physiology of the sugarcane production

• evaluation of greenhouse gas (GHG) emissions • direct and indirect impacts on land use changes• carbon storage in the soil and gaseous emissions• assessment of socioeconomic impacts• water use and impacts on water resources• geoprocessing and remote sensing • integrated analysis of sustainability

• pretreatment of lignocellulosic materials• conversion of biomass into industrial products• fractionation and characterization of biomass and its derivatives• bioprocess science and engineering• Industrial biotechnology for biomass conversion • science and engineering of chemical and biochemical reactors• scale up and validation of processes

• plant physiology• applied microbiology and biotechnology• analytical tools for biomass and derivatives• computational and system biology• enzyme production and lignocellulosic hydrolysis

• assessment of the impacts of new bioenergy technologies��RSWLPL]DWLRQ�RI�FRQFHSWV�DQG�SURFHVVHV�LQ�ELRUH´QHULHV• input-output analysis (socioeconomic impacts)• life-cycle assessment (environmental impacts)

17

FRYHUHG�ZLWK�VXJDUFDQH�WUDVK�

Research Groups: Innovation

LQ�3ODQWLQJ�DQG�+DUYHVWLQJ�3URFHV-

VHV�ZLWK�/RZ�7UDI´F��%HVW�8VHV�RI�

$JURFKHPLFDOV�DQG�5HVLGXHV��3UH-

cision Agriculture and Low Carbon

$JULFXOWXUH�

Biomass Processing

3HUIRUPV� 5',� ZLWK� WKH� JRDO�

to convert biomass into fuels, new

materials, and chemicals for seve-

UDO� LQGXVWULDO� VHFWRUV�� 5HVHDUFKHUV�

use biochemical, metabolic and

ELRSURFHVV� HQJLQHHULQJ�� FKHPLFDO�

DQG�ELRORJLFDO�FDWDO\VLV��GRZQVWUH-

DP�� SK\VLF�FKHPLFDO� VHSDUDWLRQV��

DPRQJ� RWKHU� WHFKQLTXHV� IRU� SUR-

cess proof-of-concepts at labora-

WRU\�DQG�SLORW�VFDOH�

A major differential of the

group is the Pilot Plant for Proces-

VHV�'HYHORSPHQW�XVHG�WR�VFDOH��XS�

DQG� GRZQ�� WHFKQRORJLHV�� 3URIHV-

sionals are still developing metho-

dologies for the characterization

of biomass and its derivatives and

conceptual projects processes re-

ODWHG�WR�WKH�ELRPDVV�FRQYHUVLRQ�

Research Groups: Applied

,Q� &7%(� WKH� LQWHUQDO� DJHQGD�

for scientific and technological

investigation is performed in its

five research divisions, focused

on the main bottlenecks in the

biomass / bioenergy production

F\FOH�� *HW� WR� NQRZ� WKH� UHVHDUFK�

REMHFWLYHV�LQ�HDFK�GLYLVLRQ��

Biomass Production

5HVHDUFKHUV� VWXG\� SURFHVVHV�

and solutions focused on the evo-

lution of economic and environ-

mental aspects of the agricultural

mechanization of sugarcane, with

emphasis on production costs,

VRLO� FRQVHUYDWLRQ� DQG� TXDOLW\� RI�

UDZ�PDWHULDOV�DQG�RSHUDWLRQV��7KH�

group develops a Controlled Tra-

I´F�6WUXFWXUH��(7&���ZKLFK�DLPV�WR�

UHGXFH� E\� ���� WKH� LQFLGHQFH� RI�

WUDI´F�RQ�WKH�VXJDUFDQH�´HOG��SUH-

serving soil nutrients and saving

IXHO�� 2Q� WKH� RWKHU� KDQG� DQ� DOWHU-

native harvesting process, embe-

dded to three prototypes being

WHVWHG��DLPV�WR�UHGXFH�E\�����WKH�

FRVW� RI� RSHUDWLRQ�� 2WKHU� JURXS�

projects involve planting and pre-

cision fertilizing and studies on soil

internal research

18

projects and planning investment

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Research Groups: Develo-

SPHQW� RI� WKH� 9LUWXDO� 6XJDUFDQH�

%LRUH´QHU\� �96%��� ,QQRYDWLRQ� RQ�

&RPSXWLQJ�$QDO\VLV�IRU�WKH�96%�

Sustainability of

Biomass and Bioenergy

Production

It assesses the sustainability of

the current production process in

the bioenergy sector, considering

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also includes technological innova-

tions, mainly from the contributions

RI�WKH�&7%(�LQ�WKH�DJULFXOWXUDO�DQG�

industrial phases, and the expan-

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The integrated approach of

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3URFHVVLQJ� RI� %LRPDVV�� 6FDOH� XS�

3URFHVV���3LORW�3ODQW�

Integrated Evaluation

RI�%LRUH´QHULHVIt works on the development

of a computational simulation tool

FDOOHG� 9LUWXDO� 6XJDUFDQH� %LRUH´-

QHU\� �96%��� � 7KH� 96%� HYDOXDWHV�

different new technologies and

agricultural strategies in the sugar-

cane sector through assessment

of their sustainability indicators

�HFRQRPLF��HQYLURQPHQWDO�DQG�VR-

FLDO��� 7KH� GDWD� JHQHUDWHG� E\� WKH�

tool can contribute to companies,

governments and research and

development institutions setting

priorities, assessing the success of

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metabolites present in plant tissues.

19

DV�ZHOO�DV�LWV�FRQYHUVLRQ�LQWR�OLTXLG�

fuels, materials, chemical products

and other high value-added bio-

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´F�UHVHDUFK�EDVHG�RQ�V\VWHP��FRP-

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genetic and metabolic engineering

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approaches aim at the develop-

ment, improvement and diffusion of

relevant technologies to academic

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Research Groups:� (Q]\PH�

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0HWDEROLF�(QJLQHHULQJ��)XQFWLRQDO�

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physics abd Analitical Methods for

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and Computational Biology and

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the research group exploits syner-

gies between different aspects of

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sed on a geo-referenced database

that enables the combination of

data in areas such as evaluation of

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impacts on land use changes and

carbon storage in the soil, water

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Research Groups:� (YDOXDWLRQ�

RI� (QYLURQPHQWDO� ,PSDFWV�� (YDOX-

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Functional Biology,

Biotechnology and

Biophysics

It aims unravel the mechanis-

ms of plant production and growth,

Microscopic image of the cross section of a sugarcane stem. A deep knowledge of this molecular

VWUXFWXUH�FDQ�EULQJ�JUHDW�EHQH´WV�WR�WKH�FHOOXORVLF�HWKDQRO�UHVHDUFK�DUHD�

20

CTBE develops

research in the ag-

ricultural, industrial,

technology assess-

ment, sustainability

and basic research

of biomass/ bio-

products cycle,

either independently

or in partnership

with the main bioen-

ergy companies and

research institutions.

21

&7%(­V� UHVHDUFKHUV� LVRODWHG� WZR�

QHZ� HQ]\PHV�� D� FHOOXODVH� �&HO(���

DQG�D�[\ODQDVH��6&;\O��

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served domain of glycoside hydro-

lases family 5 and a carbohydrate-

�ELQGLQJ�PRGXOH��&%0���ZKLFK�GLG�

QRW� VKRZ� VHTXHQFH� LGHQWLW\� ZLWK�

known conserved domains, sug-

gesting being a member of a new

IDPLO\� RI� &%0V�� 7KH� 6&;\O� [\OD-

QDVH� �UHSUHVHQWDWLYH� RI� JO\FRVL-

GH� K\GURODVHV� IDPLO\� ����� VKRZHG�

preference to hydrolyze unbran-

ched xylan chains, as well as the

potential to produce xylooligosac-

charides with industrial application

IURP�OLJQRFHOOXORVLF�ELRPDVV��

6HH�0RUH�

Alvarez, T. M., Goldbeck, R., dos San-tos, C. R., Paixão, D. A., Gonçalves, T. A., &DLUR�� -�� 3�� /�� )��� ���� � 6TXLQD�� )��0�� ��������Development and Biotechnological Ap-plication of a Novel Endoxylanase Family *+���,GHQWL´HG�IURP�6XJDUFDQH�6RLO�0HWD-genome. PloS one, 8(7), e70014.

7KH� 5'� VWXGLHV� SHUIRUPHG�

LQ�WKH�&7%(�DLP�WR�JHQHUDWH�VLJ-

nificant impacts in several areas

related to the biomass and bio-

HQHUJ\� SURGXFWLRQ�� 7KH� UHVHDU-

chers effort contribute to improve

agricultural, industrial and sustai-

nability indicators of the produc-

WLRQ�F\FOH��2Q�WKH�IROORZLQJ�SDJHV�

some outstanding works of the

laboratory in the year 2013 will be

IHDWXUHG�

,GHQWL´FDWLRQ�DQG&KDUDFWHUL]DWLRQ�RI�DCellulase and a Xylanase

IURP�0HWDJHQRPLF�2ULJLQ��

The discovery of new glycolytic

enzymes is of great value to the

development of renewable ener-

J\� VRXUFHV�� 0HWDJHQRPLFV� LV� DQ�

important tool for the discovery

of new genes that encode enzy-

mes of biotechnological relevance

in the genome of uncultured mi-

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screening of a metagenomic li-

brary derived from sugarcane soil,

research highlights

22

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The main production operations

of sugarcane are related to planting

and harvesting and mechanical ac-

tions involve heavy mechanical han-

dling actions of biomass, inputs and

VRLO�� &7%(� GHYHORSV� D� SURMHFW� WKDW�

promotes several improvements

to the agricultural management of

sugarcane, among them a new pro-

cess of harvest that, in 2013, was

´HOG�WHVWHG� ZLWK� WZR� SURWRW\SHV�

FRXSOHG� WR� D� WUDFWRU�� 6XFK� HTXLS-

ment seeks to reduce the severe

restrictions that the current harves-

WLQJ�SURFHVV�UHTXLUHV�WR�WKH�DJURQR-

mic management of the plantation,

improving productivity and econo-

mic and environmental sustainabili-

W\�RI�VXJDUFDQH�SURGXFWLRQ�

The centerpiece of the machi-

nery of low impact mechanization

RI�WKH�&7%(��FDOOHG�&RQWUROOHG�7UD-

I´F�6WUXFWXUH��(7&���DOVR�HQWHUHG�LQ�

WKH�WHVWLQJ�SKDVH��,W�VHHNV�WR�UHGXFH�

the restrictions on planting space,

soil compaction, fuel consumption,

and losses of biomass and levels of

impurities in the raw material during

KDUYHVW��,W�ZLOO�DOVR�GHFUHDVH�LQYHVW-

ment costs and maintenance by

increasing the number of simulta-

neous lines of planting and harves-

WLQJ�LQ�QHZ�HTXLSPHQW�

6HH�0RUH�

Funtec / BNDES project: investments RI�5�����PLOOLRQ�RYHU�´YH�\HDUV��LQ�SDUWQHUVKLS�ZLWK�-DFWR��)RXU�SDWHQWV�KDYH�EHHQ�UHTXLUHG�

23

1HZ�6SHFLHV�RI�Yeast with High

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&7%(­V� 5HVHDUFKHUV� KDYH�

identified a new species of yeast

in the intestinal tract of larvae

RI� WKH� FKU\VRPHOLG� EHHWOH�� 7KH�

Pseudozyma brasiliensis� VS�� QRY�

is able to metabolize five-carbon

sugars and secrete xylanase with

activity 20 times higher than the

enzyme extract from Aspergillus

niger, fungus notoriously recog-

nized by the expression of this

W\SH�RI�HQ]\PH��

This xylanase can be used in

the degradation of the bagasse

fiber of sugarcane for conversion

of cellulosic ethanol, as well as pa-

SHU�� IRRG� DQG� IHHG� LQGXVWU\�� 0R-

reover, it also has the potential to

be used in the production of xylo-

oligosaccharides, prebiotics that

SURPRWH�PDQ\�KHDOWK�EHQHILWV�

*LYHQ� WKH� ELRWHFKQRORJLFDO�

potential of this yeast, the ge-

nome of 20 million base pairs

ZDV� VHTXHQFHG� DQG� SXEOLVKHG�

UHFHQWO\�� :H� DUH� FXUUHQWO\� SHU-

IRUPLQJ� 51$6HT� DQDO\VHV� RI�

this Pseudozyma in order to

identify important genes in the

metabolism of xylose and other

SRO\VDFFKDULGHV�

6HH�0RUH�

Oliveira, J. V. C, et al. (2013). Draft ge-QRPH�VHTXHQFH�RI�3VHXGR]\PD�EUDVLOLHQ-sis sp. nov. strain GHG001, a high producer of endo-1, 4-xylanase isolated from an in-sect pest of sugarcane. Genome Announ-cements, 1(6), e00920-13.

24

(Q]\PDWLF�&RPSOH[&RPLQJ�IURP�$PD]RQ

&7%(­V� UHVHDUFKHUV�KDYH� LGHQ-

WL´HG� D� Trichoderma harzianum

enzymatic complex isolated from

Amazon biome with high biotech-

nological potential for the second

JHQHUDWLRQ� HWKDQRO� SURGXFWLRQ��

Advanced molecular biology and

ELRSURFHVV�HQJLQHHULQJ�WHFKQLTXHV�

were used in order to optimize the

production process of the enzy-

mes complex in a joint effort with

(PEUDSD�,QVWUXPHQWDWLRQ��7KH�SUR-

MHFW� UHFHLYHG� LQYHVWPHQWV� RI� 5��

��������IURP�&13T�DQG�KDV�UHVXO-

ted in the publication of a patent

DQG�VHYHUDO�SDSHUV�

Professionals of the same rese-

arch division have prepared experi-

ments in laboratory and pilot scale in

order to develop and demonstrate

WKH� 9HU\� +LJK� *UDYLW\� �9+*�� )HU-

mentation Process of sugarcane jui-

ce and molasses with high ethanol ti-

WHU�DQG�D�GHFUHDVH�RI�RYHU�����LQ�WKH�

DPRXQW� RI� YLQDVVH� JHQHUDWHG�� 7KLV�

research, conducted in partnership

with a company, used sensors de-

veloped to monitor the fermentation

SURFHVV�LQ�UHDO�WLPH��7KH�SHUIRUPDQ-

ce of yeasts was evaluated, including

JHQHWLFDOO\�PRGL´HG�RUJDQLVPV�

6HH�0RUH�

'HODERQD�� 3�� '�� 6�� HW� DO� �������� 8Q-derstanding the cellulolytic system of Tri-choderma harzianu P49P11 and enhancing saccharification of pretreated sugarcane bagasse by supplementation with pectina-se and Ѓ-l-arabinofuranosidase. Bioresour-ce Technology, 131, 500-507.

25

(PLVVLRQV�RI�*UHHQKRXVH*DVHV�LQ�WKH�%UD]LOLDQ3URGXFWLRQ�RI�(WKDQRO

Mechanical harvesting has re-

placed manual cutting with bur-

ning of sugarcane due to, among

other factors, greenhouse gases

�*+*�� DQG� SDUWLFXODWH� PDWWHU�

emissions that cause public heal-

WK� DQG� HQYLURQPHQWDO� SUREOHPV��

This change in management leads

to the deposition of 10 to 20 to-

nes of dry matter of crop residue

SHU� KHFWDUH� KDUYHVWHG�� %HVLGHV�

preventing emissions by biomass

burning, mechanized harvesting

ZLWKRXW�VWUDZ�UHPRYDO�E\�´UH�WHQ-

ds to increase the stock of soil car-

bon, a sink of more than one ton of

FDUERQ�SHU�KHFWDUHDQQXDOO\�

&7%(� KDYH� HYDOXDWHG� WKH�

impact of this change of mana-

JHPHQW� IRFXVLQJ� RQ� *+*� HPLV-

sions and storing carbon in the

soil, through field experiments

and remote sensing, environmen-

tal modeling and life cycle asses-

VPHQW� WRROV�� 2XU� UHVHDUFK� LQ� WKLV�

area was recognized with the 2013

7RS�(WKDQRO�$ZDUG��3URIHVVLRQDOV�

have also assessed the impacts

of removing the straw that is cur-

rently in the field for cogeneration

and production of lignocellulosic

HWKDQRO���*��

6HH�0RUH�

Galdos, M. et al. (2013). Trends in global warming and human health impacts related to Brazilian sugarcane ethanol production considering black carbon emissions. Applied Energy, 104, 576-582.

26

9LUWXDO�6XJDUFDQH�%LRUH´QHU\��96%�

,Q� ������ &7%(�ZRUNHG� RQ� LP-

SURYLQJ� WKH� 96%� PRGHOV� IRU� GL-

IIHUHQW� FRQ´JXUDWLRQV� ELRUH´QHU\�

systems considering technical

economic, social and environmen-

WDO�DVSHFWV�

Among these improvements,

straw recovery and transporta-

WLRQ� V\VWHPV� � ZHUH� HYDOXDWHG�� $�

paper was published evaluating

various straw recovery scenarios

considering the integration of

DJULFXOWXUDO�DQG�LQGXVWULDO�SKDVHV��

Best technical and economic te-

chnologies were identified and

comprehensive life cycle inven-

tory for environmental assess-

PHQW�ZDV�REWDLQHG�

(YDOXDWLRQ� RI� GLIIHUHQW� WHFK-

nological routes were performed

XVLQJ� WKH� 96%�� H�J�� LQWHJUDWLRQ� RI�

VHFRQG� JHQHUDWLRQ� HWKDQRO� �ELR-

FKHPLFDO� URXWH�� WR� WKH� ´UVW� JHQH-

ration plant, alcoholchemistry for

butanol production, thermoche-

PLFDO� IRU� ELRIXHOV�� DPRQJ� RWKHUV��

)XUWKHUPRUH��D�PHWKRG�WR�LGHQWLI\�

the most relevant agronomic and

industrial variables for optimizing

integrated processes was also de-

YHORSHG�DQG�LPSOHPHQWHG��

)XUWKHU� ZRUN�� LQWHJUDWLRQ� RI�

product use and social impact

DVVHVVPHQW�

6HH�0RUH�Cardoso, T. et al. (2013). Technical and

economic assessment of trash recovery in the sugarcane bioenergy production sys-tem. Scientia Agricola, 70(5), 353-360.

Agricultural

Production Modelling

Distribution and Use Model

Economic

Evaluation

Social

Evaluation

Environmental

Evaluation

Economic

Engineering

Input-output

Analysis

Life-cycle

Assessment

27

3URWRFRO�)$,5(�6HT�IRU�6XJDUFDQH�*HQRPH�

5HVHDUFKHUV�� FXUUHQWO\� RI� WKH�

&7%(�DQG�WKH�1DWLRQDO�/DERUDWRU\�

IRU�%LRVFLHQFHV��/1%LR��KDYH�SDU-

ticipated in the development of a

)$,5(�6HT�SURWRFRO��)RUPDOGHK\-

GH�$VVLVWHG�,VRODWLRQ�RI�5HJXODWRU\�

(OHPHQWV��$FURQ\P�LQ�(QJOLVK��DS-

plied to unicellular Clamydomonas

alga and to the vascular plant Ara-

ELGRSVLV��6XFK�PHWKRGRORJ\�XVHG�

in human cells allows identifying

regions in the DNA important to

the regulation of expression levels

�WUDQVFULSW��RI�WKH�RUJDQLVP�JHQHV��

The transcription regulation is im-

portant to the control and manipu-

lation of genetic characteristics of

SODQWV�RI�LQWHUHVW�WR�&7%(��VXFK�DV�

mass and sugar accumulation and

JURZWK�HWF�

The developed protocol was

SXEOLVKHG�LQ�WZR�VFLHQWL´F�DUWLFOHV�LQ�

������'XULQJ�WKH�VDPH�\HDU��SURIHV-

sionals of the Laboratory have deve-

loped a computational platform for

the analysis of such data, facilitating

the handling of information by bio-

logists without training in program-

PLQJ�� ,Q�������WKH�WHFKQLTXH�ZLOO�EH�

adapted to sugarcane, a complex

achievement, since this plant does

QRW�KDYH�WKH�VHTXHQFHG�JHQRPH�

6HH�0RUH�Winck, F. V. et al. (2013). Genome-

�ZLGH�LGHQWL´FDWLRQ�RI�UHJXODWRU\�HOHPHQWV�and reconstruction of gene regulatory ne-tworks of the green alga Chlamydomonas reinhardtii under carbon deprivation. PloS One, 8(11), e79909.

28

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sment of sugarcane trash for agro-

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relevant success indicator of the

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articles in indexed journals, which

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warming and human health im-

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ne ethanol production considering

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derstanding the cellulolytic system

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of pretreated sugarcane bagasse

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selectedpublications

29

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30

High-performance

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alcohols and organic

acids developed by

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tives of the CTBE.

31

'LUHFWRU�RI�&7%(Carlos Alberto Labate

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Biomass Production

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tegrated Assessment

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tainable of Biomass and Bioenergy

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and Biotechnology

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ployees, including contractors and

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undergraduate research to pos-

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consultants, coming from renow-

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professionals

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32

)iELR�0DUFLR�6TXLQDKWWS���ODWWHV�FQST�EU������������������

Pharmacist graduated from

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PhD in molecular biology from Medi-

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in fungal biochemistry and genetics,

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cular biology, biochemistry, ‘Omics’

6FLHQFHV�DQG�KLJK�WKURXJKSXW�VFUH-

HQLQJV��+LV�UHVHDUFK�LV�DLPHG�DW�WKH�

development of enzymes and biote-

chnological routes for plant biomass

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5HVHDUFKHUVLearn about the professional

education and work developed by

some of the leading researchers of

WKH�&7%(�

33

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*UDGXDWHG� LQ�$JURQRPLF�(QJL-

QHHULQJ��DW�81(63�-DERWLFDEDO��+RO-

ds a doctorate degree in soils and

SODQW�QXWULWLRQ�E\�(6$/4�863��ZLWK�

a postdoctoral in the use of fertili-

zers enriched in 15N, 346�DQG�10B in the

assessment of management form

RI�QXWULHQWV� LQ�VXJDUFDQH��+H�ZDV�D�

researcher visitor at the University

RI�4XHHQVODQG��$XVWUDOLD���:RUNV�DW�

&7%(� VLQFH� ����� LQ� UHVHDUFKHV� RQ�

the agronomic management of ferti-

lizers, soil conditioners and industrial

residues in the nutrition of sugarca-

ne, development of special fertilizers

DQG�SUHFLVLRQ�DJULFXOWXUH�

0DULD�7HUHVD�%RUJHV�3LPHQWD�%DUERVDChemist, works in the area of pre-treatment and physicochemical analyzes of sugarcane bagasse for bio-

ethanol production.

0DULD�7HUHVD�%RUJHV�3LPHQWD�%DUERVDKWWS���ODWWHV�FQST�EU������������������

+ROGV� D� EDFKHORU­V� GHJUHH� LQ�

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ty of Uberlândia, with a Master’s and

PhD in Physico-Chemistry from the

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ded exchange scholarship doctorate

DW�,QVWLWXW�1DWLRQDO�3RO\WHFKQLTXH�GH�

*UHQREOH� �)UDQFH�� DQG� SRVWGRFWR-

UDO�DW�8QLYHUVLGDG�GH�*LURQD��6SDLQ���

6KH�LV�D�UHVHDUFKHU�DW�WKH�&7%(�VLQ-

FH�������ZKHUH�VKH�RSHUDWHV�LQ�WKH�

area of characterization and pro-

cessing of biomass and its deriva-

tives for the production of cellulo-

sic ethanol, chemical intermediates,

EXLOGLQJ�EORFNV�DQG�RWKHU�SURGXFWV�

34

0DUFHOR�9DODGDUHV�*DOGRVKWWS���ODWWHV�FQST�EU������������������

*UDGXDWHG� LQ� ,QWHUQDWLRQDO�

Agricultural Development from

Midamerica Nazarene University

�(8$��DQG�REWDLQHG�D�GRFWRUDWH�LQ�

Agronomy from the University of

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WGRFWRUDO� UHVHDUFK� DW� &(1$�863�

in Life Cycle Assessment of agri-

FXOWXUDO� SURGXFWV�� &XUUHQWO\�� UHVH-

arches soil and biomass carbon

dynamics and greenhouse gas

emissions in biofuel production,

using measurements, geoproces-

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+LV�PDLQ�DUHDV�RI�UHVHDUFK�DUH�ODQG�

use change and sustainable agri-

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tended postdoctoral in Lunds

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12 years experience in processing

RI� ELRPDVV�� $W� &7%(�� VKH� ZRUNV�

in the area of pre-treatment and

physicochemical characteriza-

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&DSHV�*UDQG�3UL]H�RI�7KHVLV�*UH-

DW�LQ�WKH�DUHDV�RI�([DFW�DQG�(DUWK�

6FLHQFHV�

6DULWD�&kQGLGD�5DEHORGraduated in chemistry, works with pre-treatment and chemical characterization of biomass. His doctoral the-

sis won the Capes Grand Prize of Thesis.

35

2WiYLR�&DYDOHWWKWWS���ODWWHV�FQST�EU������������������

)RRG� (QJLQHHU� IURP� )HGHUDO�

8QLYHUVLW\� RI� 5LR� *UDQGH� �)85*���

3K'�LQ�WKH�VDPH�DUHD�DW�6WDWH�8QL-

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archer at Parthenope University of

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WKH�'HSDUWPHQW�RI�5XUDO�DQG�8UEDQ�

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RI� $JULFXOWXUDO� 6FLHQFHV� �6/8��� $W�

&7%(��KH�ZRUNV�ZLWK�HQYLURQPHQWDO�

and social impact assessment and

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that evaluates the integration of

new technologies in the sugarcane

SURGXFWLRQ�FKDLQ�

Carlos Driemeier

KWWS���ODWWHV�FQST�EU������������������

+H� LV�D�SK\VLVW��ZLWK�D�JUDGX-

ation and doctorate degree in the

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an exchange scholarship doctora-

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The postdoctorate was conducted

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YLURQPHQWRI�WKH�8QLYHUVLW\�RI�6mR�

3DXOR� �863��� +H� KDV� H[SHULHQFH�

in Condensed Matter Physics, es-

pecially with surfaces, interfaces,

physica-chemical of the water and

FU\VWDOORJUDSK\��$QG�D�UHVHDUFKHU�

RI�WKH�&7%(�VLQFH�������ZKHUH�KH�

develops analytical methods and

investigates the nanostructure of

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Carlos Driemeier

Physicist, expert in nanostructure of lignocellulosic biomass and characterization methods related to nano-

structural variability.

36

Biomass Physical

7UHDWPHQW�8QLW�LQ�

this Pilot Plant for

Process Develop-

ment (PPDP).

37

�� (OL�/LOO\���(ODQFR

• Dow

• Odebrecht Agroindustrial

• University of Nottingham

• Bunge

Highligths of Established

Partnerships

Rhodia: Chemical intermedia-

tes obtention from macromole-

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SURMHFW� LV� ´QDQFHG� E\� %1'(6�

�)XQWHF���5������PLOOLRQ��DQG� LV� LQ�

LWV�WKLUG�\HDU�RI�GHYHORSPHQW��7KH�

biotechnological process was sca-

led up at the Pilot Plant for Pro-

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FRQG�ZRUN��7KH�LQYHVWPHQW�LQ�WKLV�

LQLWLDWLYH�ZDV� DURXQG�5����PLOOLRQ�

LQ�QLQH�PRQWKV�

Dow: Production of chemical

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last two and a half years and has

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%1'(6�),1(3��

Eli Lilly (Elanco): Biomass

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WHPEHU� ������ D� ���PRQWKV� DJUH-

ement was signed, with an in-

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&7%(�%XVLQHVV�0DQDJHPHQW�

area works in the development

of partnerships with research

institutions, industries and capi-

tal markets in the areas of bio-

energy and renewable materials

to create favorable conditions

for technology transfer, access

provision to the knowledge pro-

duced in the Laboratory and its

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13 cooperation agreements were

signed with various institutions,

whereas others reached an ad-

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Major partnerships in 2013

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ments and memoranda of unders-

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�� 5KRGLD

�� 'RZ���'60

• BP

�� *(

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�� -DFWR�$JULFXOWXUDO�0DFKLQHV

�� (PEUDSD

�� ,&21(

• Unicamp

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innovation

38

�����DQG�������7KH�DFWLYLWLHV�RI�WKH�

research cover the development

of Base Machine of the Controlled

7UDI´F�6WUXFWXUH��(7&��DQG�LWV�KDU-

vest heads, agronomic evaluation

of the precise sugarcane planting

SURFHVV��DPRQJ�RWKHUV�

GE:� (YDOXDWLRQ� RI� HWKDQRO�

SURGXFWLRQ� WHFKQRORJ\�� 3UR-

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DQ� LQYHVWPHQW� RI� 5�� ��������

PDGH�E\�*(�

Odebrecht Agroindustrial:

Technical, economic and environ-

mental assessment of a process

for second generation ethanol

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Unicamp / SAT / Fitroleum:

(YDOXDWLRQ� RI� H[LVWLQJ� URXWHV� IRU�

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),1(3�DQG�D�5������PLOOLRQ�PLQL-

mum non-financial contribution

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high alcoholic content sugar wi-

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million, half funded by BP and half

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FDOOHG� ª$VVHVVLQJ� :DWHU� 5HVRXU-

FHV� LQ� 'DWD� /LPLWHG� 5HJLRQV� DQG�

Changing Land Use” is under de-

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ximately one and a half year and

ZLWK�D�EXGJHW�RI�5�����������

Jacto Agricultural Machines:

Low impact mechanization for the

production of sugarcane, with an

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E\� %1'(6� �� )817(&� EHWZHHQ�

39

3ODQW�0DWHULDO�DQG�&RPSRXQG�

�� %5���������������� %LIXQF-

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�� %5����������������Trichoder-

ma harzianum�(Q]\PDWLF�&RFNWDLO�6X-

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Complex for the Polysacccharide

+\GURO\VLV�DQG�,WV�8VHV�

Patents

In 2013, the professionals of

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National Institute of Industrial Pro-

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DUH�OLVWHG�EHORZ�

�� %5���������������� '\QD-

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RI�6HHG�6XJDUFDQH�'RVLQJ�IRU�3UH-

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�� %5���������������� 'HYLFH�

of Parallel Plates for Alignment of

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�� %5����������������6\VWHP�

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FHVV�IRU�WKH�3URGXFWLRQ�RI�D�)XUDQLF�

Carboxilic Acid Compound from a

40

Workshop

promoted by the

CTBE in Decem-

ber 2013, brought

together representa-

tives from research

institutions, com-

panies and govern-

ments of Brazil and

Denmark. They ex-

plore possibilities for

joint developments

of technologies that

exploit the concept

RI�ELRUH´QHULHV�

41

About 100 people participat-

HG�WKH�ZRUNVKRS�

• II Workshop on Sugarcane

Physiology for Agronomic

Applications

October, 2nd and 3rd.

Brought together leading re-

searchers from Brazil and abroad

to discuss the sugarcane physi-

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professionals attended the event,

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LQ������

• First Brazilian-Danish

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December, 2nd ad 3rd

The event explored joint pos-

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search and development in the area

of new technologies that promote

WKH� FRQFHSWV� RI� ELRUH´QHULHV� DQG�

ELREDVHG� HFRQRP\�� 7KH� ZRUNVKRS�

also celebrated the launch of the

new Postgraduate program in Bio-

energy, a collaboration between

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Courses

• Short Course Principles

&7%(� SURPRWHV� WUDLQLQJ� DF-

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International workshops and

other events are organized fre-

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results and improve scientific col-

laborations towards the bioen-

ergy development in Brazil and

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KHOG�DW�&7%(��LQFOXGLQJ�

International Workshops

Promoted in 2013

• Workshop on Second

Generation Bioethanol 2013:

Enzymatic Hydrolysis

November, 11th and 12th

This was the fourth edition

of the event which takes place at

&7%(�DQQXDOO\�VLQFH�������7KH�GLV-

cussions have focused on issues

OLNH�� K\GURO\VLV� SURFHVVHV� FDUULHG�

out by fungi, relationships between

structure and function of enzymes,

bioprocesses, metagenomics ap-

plied to biofuels, protein production

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WRXV�IXQJL���SUH�WUHDWPHQW�YV��HQ]\-

PDWLF�K\GURO\VLV��DPRQJ�RWKHUV�

events and training programs

42

nanometric porosity of water

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losic biomasses, this porosity is

a key factor of reactivity and en-

]\PDWLF� GLJHVWLELOLW\�� 7KH� FRXUVH�

objective was to disseminate this

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VHDUFK�JURXSV�

• Training Course on

Oxygen Transfer

Divided into theoretical and

practical modules, was taught to

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8QLFDPS��'XUDWLRQ����K�

Programs for Students

8QL´HG� ,QWHUQVKLS� 3URJUDP�

and Applications of

System Biology and

Plant Physiology

February and November

Designed for postgraduate

students, the course aimed to dis-

seminate approaches in system bi-

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held in 2013, in partnership with the

postgraduate programs in Vegetal

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XODU�%LRORJ\�RI�8QLFDPS��7KH�HYHQW�

ZDV�DWWHQGHG�E\����VWXGHQWV�

• Short course of

Thermoporometry Applied

to Lignocelluloses

July, 25th

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ZKR�OHDUQHG�DERXW�WKH�WHFKQLTXH�

GHYHORSHG� LQ� &7%(� WR� PHDVXUH�

Speaker presentation at the fourth edition of the Workshop on Second Generation Bioethanol:

Enzymatic Hydrolysis, in CTBE Auditorium.

43

period which the selected stu-

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There are, on average, 20 vacan-

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)XUWKHU�LQIRUPDWLRQ�

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tion Scholarship Program (PIBIC):

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arships of the National Council for

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YHORSPHQW� �&13T��� 7KH� FDOO� IRU�

registrations takes place once a

year, with an average of 20 stu-

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tion of twelve months, beginning

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(PUE): high school and under-

graduate students may partici-

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like Physics, Chemistry, Biology,

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call for registrations takes place

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Summer School Program:

Intended for undergraduate stu-

dents from Latin America and the

Caribbean who have completed at

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5HJLVWUDWLRQV� DUH� RSHQ� XVXDOO\� LQ�

-XO\�DQG�WKH�SURJUDP�LV�FRQGXFW-

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Participants for the Summer School Program, in which graduate students from all over Latin

America come annually to the CNPEM to develop research projects during the months of Janu-

ary and February.

44

A biotechnological process was

validated in semi-industrial scale,

making bioreactors and utilities

of the Pilot Plant work 24 hours

a day, 7 days a week for seven

months, attesting its proper

commissioning.

2013 in two pages

Facilities of the Metabolomics

and Computational and

Evolutionary Biology Laboratory

have been concluded, and the

Analytical Center for Biomass

and Derivatives were structured,

This establishes platforms for

TXDQWL �FDWLRQ�RI�PHWDEROLWHV�

and lipids in complex biological

samples.

A new species of yeast with high

biotechnological potential has

EHHQ�LGHQWL �HG�LQ�WKH�LQWHVWLQDO�

tract larvae of the chrysomelid

beetle. A Pseudozyma brasiliensis

sp. nov. is able to metabolize

�YH�FDUERQ�VXJDUV�DQG�VHFUHWLQJ�

xylanase.

Facilities of the Metabolomics

and Computational and

Evolutionary Biology Laboratory

have been concluded, and the

Analytical Center for Biomass

and Derivatives were structured,

45

In December, the Laboratory

RSHQHG�LWV� �UVW�WZR�5'�IDFLOLWLHV�

for external research, through

on-line submission proposals on

8VHU�3RUWDO�RI�&13(0��7KH\�DUH��

Process Development Labs (LDP)

and Pilot Plant.

At the end of the year, circulation

in patio and deployment of

automation of base machine of

WKH�&RQWUROOHG�7UDI �F�6WUXFWXUH�

(ETC) were initiated. Their harvest

heads were also improved

in experiments performed in

VXJDUFDQH� �HOGV�

CTBE became a partner of

CANASAT Program, originating

from the National Institute

for Space Research (INPE).

Professionals of the Laboratory

will produce data for the tool

related to GHG emissions, social

and economic impacts of the

sugarcane crop.

CTBE became a partner of

CANASAT Program, originating

from the National Institute

for Space Research (INPE).

Professionals of the Laboratory

will produce data for the tool

46

Brazilian Center for Research in

Energy and Materials (CNPEM)

Edition: /XL]�3DXOR�-XWWHO���$&2Graphic Design and Layout: William Barbosa / ACO

Printing: *Ui´FD�&DPSLQDV�H�(GLWRUD���,*&

Photos: *LDQFDUOR�*LDQQHOOL��*XLOKHUPH�%RULQL��*XVWDYR�7LOLR�

/XL]�3DXOR�-XWWHO�DQG�3DEOR�/HYLQVN\

Campinas, Sao Paulo - June 2014

47

48