Reverse  Vaccinology    

Wage  Indrya  Nugraha  

Edward  Jenner  1796  h=p://www.wired.com/science/discoveries/news/2008/05/dayintech_0514#  

Louis  Pasteur  1886    

h"p://www.icollector.com/Louis-­‐Pasteur_i9688850  

Reverse  Vaccinology  1.  Analisis  komputer  (In  Silico)  2.IdenRfikasi  anRgen    3.  Animal  test  4.  Molecular  epidemology  5.  Uji  klinis  6.  Ijin  dari  regulator  7.  Rekomendasi  8.  Implementasi  

Rappuoli  R.  (2010).  Reverse  vaccinology.  Immunity  Review  33  :  530-­‐541.    

Vaccinomic    

Rappuoli,  R  (2000).  Reverse  Vaccinology.  Genomics.  445-­‐450.  

Reverse  Vaccinology  

Biology  Molekuler,  Immunology,  Genomic  (-­‐Omic),  Komputasi  &  BioinformaRc.  

 

Reverse  Vaccinology  

Epitope  ;  an=genic  determinant    Bagian/sisi  anRgen  Yang  dikenali  oleh  reseptor  AnRgen/AnRbodi.    AnRgen  :  Molekul  yang  dikenali  oleh  sistem  kekebalan  tubuh    

Reverse  Vaccinology  

Reverse  Vaccinology  

Epitope  Linier  &    Epitope  Non  Linier    

Reverse  Vaccinology  

Reverse  Vaccinology  

Reverse  Vaccinology  

BioinformaRc    Public  Domain,  Desktop  Applica=on  (Free  access-­‐proprietary)  :    IEDB,  ELLIPRO,  GENEIOUS,  CLC-­‐BIO,  EPIVAX,  VAXIGN  

Vaccinomic    

Reverse  Vaccinology  

Grup  B  Meningococcus,  Malaria,  Tuberculosis,  Syphilis,  HepaRRs-­‐B,  HepaRRs-­‐C  

Daiar  Pustaka  H=p://www.epivax.com  .  h=p://www.immuneepitope.org/  .  De  Groot,  Anne  S.,  Cohen,  Tobias.,  Ardito,  Ma=hew.,(2010)  Use  of  BioinformaRka  to  Predict  MHC  Ligands  and  T-­‐Cell  Epitopes:  ApplicaRon  to  Epitope-­‐Driven  Vaccine  Design.  Methods  in  Microbiology.37:35-­‐66.  Rappuoli,  R.,  Se=e,  Alessandro.,  (2010).  Reverse  Vaccinology:Developing  Vaccines  in  The  Era  of  Genomics.  Immunity.  33,  530-­‐541.  Vivona,  Sandro.,  Gardy,  J.L.,  (2008).  Computer-­‐aided  biotechnology:  from  immun-­‐informaRcs  to  reverse  vaccinology.Trends  in  Biotechnology.  26,  190-­‐200.    Von  Reyn,  Fordham.,  Vuola,  Jenni.,  (2002)  New  Vaccine  for  the  PrevenRon  of  Tuberculosis.  Vaccine  35:465-­‐474.  WHO,  (2011)  WHO  Report  ;  Global  Tuberculosis  Control  2011.  France.  Xiong,  Jin.,  (2006)  Essen=al  Bioinforma=cs.  Cambridge  University  Press.  New  York.          

TERIMAKASIH    

PT. Bio Farma Vaccine & Sera Manufacturer ; Dedicated to Public Health since 1890 �