reversing a model of parkinson’s disease with in situ ...10.1038... · long exposure 72 kd 52kd...

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Reversing a model of Parkinson’s disease with in situ converted nigral neurons Hao Qian, Xinjiang Kang, Jing Hu, Dongyang Zhang, Zhengyu Liang, Fan Meng, Xuan Zhang, Yuanchao Xue, Roy Maimon, Steven F. Dowdy, Neal K. Devaraj, Zhuan Zhou, William C. Mobley, Don W. Cleveland & Xiang-Dong Fu In the format provided by the authors and unedited Supplementary information https://doi.org/10.1038/s41586-020-2388-4 Nature | www.nature.com/nature

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Page 1: Reversing a model of Parkinson’s disease with in situ ...10.1038... · Long exposure 72 KD 52KD 42 KD. 0d 2d 4d 7d 14d 21d anti-ACTB anti-nPTB 0d 2d 4d 7d 14d 21d anti-ACTB anti-PTB

Nature | www.nature.com | 1

Article

Reversing a model of Parkinson’s disease with in situ converted nigral neurons

Hao Qian, Xinjiang Kang, Jing Hu, Dongyang Zhang, Zhengyu Liang, Fan Meng, Xuan Zhang, Yuanchao Xue, Roy Maimon, Steven F. Dowdy, Neal K. Devaraj, Zhuan Zhou, William C. Mobley, Don W. Cleveland & Xiang-Dong Fu ✉

In the format provided by the authors and unedited

Supplementary information

https://doi.org/10.1038/s41586-020-2388-4

Nature | www.nature.com/nature

Page 2: Reversing a model of Parkinson’s disease with in situ ...10.1038... · Long exposure 72 KD 52KD 42 KD. 0d 2d 4d 7d 14d 21d anti-ACTB anti-nPTB 0d 2d 4d 7d 14d 21d anti-ACTB anti-PTB

anti-Actb

Figure 1 panel c

50KD

37KD

70 KD

Figure 1 panel d

anti-Actb

anti-PTB

0d 2d 4d 7d 14d 21d

0d 2d 4d 7d 14d 21d

0d 2d 4d 7d 14d 21d

Short exposure

Long exposure

50KD

37 KD

Page 3: Reversing a model of Parkinson’s disease with in situ ...10.1038... · Long exposure 72 KD 52KD 42 KD. 0d 2d 4d 7d 14d 21d anti-ACTB anti-nPTB 0d 2d 4d 7d 14d 21d anti-ACTB anti-PTB

Figure 7 panel a

37 KD

mAstrocyte+PTB-ASO

1# 2# 3# 4# 5#

Note: Red boxes indicate thebands usedfor mainfigure-4.

Short exposure

Long exposure

anti-ACTB

anti-BRN2

70 KD

50KD

37 KD

ExtendeddataFigure 1 panel c

anti-Actb

anti-PTB

Page 4: Reversing a model of Parkinson’s disease with in situ ...10.1038... · Long exposure 72 KD 52KD 42 KD. 0d 2d 4d 7d 14d 21d anti-ACTB anti-nPTB 0d 2d 4d 7d 14d 21d anti-ACTB anti-PTB

0d 2d 4d 7d 14d 21d

0d 2d 4d 7d 14d 21d

0d 2d 4d 7d 14d 21d

anti-ACTB

anti-nPTB

Short exposure

Long exposure

ExtendeddataFigure 1 panel eHDF

70KD

50KD

37KD

70 KD

50 KD

37 KD

ExtendeddataFigure 1 panel ecortical astrocyte

0d 2d 4d 7d 14d 21d

0d 2d 4d 7d 14d 21d

0d 2d 4d 7d 14d 21danti-Actb

anti-nPTB

Short exposure

Long exposure

72 KD

52KD

42 KD

Page 5: Reversing a model of Parkinson’s disease with in situ ...10.1038... · Long exposure 72 KD 52KD 42 KD. 0d 2d 4d 7d 14d 21d anti-ACTB anti-nPTB 0d 2d 4d 7d 14d 21d anti-ACTB anti-PTB

0d 2d 4d 7d 14d 21d

anti-ACTB

anti-nPTB0d 2d 4d 7d 14d 21d

anti-ACTB

anti-PTB

ExtendeddataFigure 1 panel ehuman astrocyte

70 KD

50KD

37 KD

70 KD

50KD

37 KD

ExtendedDataFig.18panel c

anti-Actb

anti-TH