review 3 c483 spring 2013. net outcomes of oxidative phosphorylation (shuttles) fatty acid...
TRANSCRIPT
Review 3
C483 Spring 2013
Net outcomes of
• Oxidative phosphorylation (shuttles)• Fatty acid catabolism• Nitrogen catabolism• Light reactions of photosynthesis• Calvin cycle
Structures to know
• All amino acids• (deoxy)ribonucleosides and
(deoxy)ribonucleotides• Carbamoyl phosphate and urea• Pyruvate, oxaloacetate, a-ketoglutarate• PRPP
Metabolic Pathways/Reactions
• Know these reactions/pathways (not arrow mechanisms)– Beta oxidation– PLP-mediated transamination– Formation of carbamoyl phosphate– Catabolism of amino acids covered in lecture– Basic hydrolysis and RNase-catalyzed hydrolysis of
RNA
Protonmotive Force
• NADH + H+ + ½ O2 NAD+ + H2O + 10 H+ pumped
• succinate + ½ O2 fumarate + H2O + 6 H+ pumped
-O
NH2
O
-O
O
O
-O
O
O
COO-
-O
NH2
O
COO-
NH
O
R O-
NH
H2N
R O-
+
NH
O
R O-
+
-O
O
O
COO-
-O
NH2
O
COO-
-O
NH
O
COO-
NAD(P)+ NAD(P)H
H2O
+ NH3
O
NH2H2N
bicarbonate
ammonia(from glutamate)
aspartate(can be derived from glutamate via transmination)
O
O
O
O
O
O
O
O
O
O
O
O
O
OO
O
OH
P
O
O
O
NH
O
ONO
OHOH
HH
HH
OP-O
O-
O
NH
O
ONO
OHOH
HH
HH
OP-O
O-
O
F
Photosynthesis: Net Reaction
2 H2O + 4 NADP+ + 8 photons O2 + 4 NADPH
Proton Gradient
• For every ONE O2
– 4 e- passed– 8 photons absorb– 12 H+ pumped
• In plants, 12 H+ required to turn ATP synthase– 3 ATP made
• Quantum yield is 3 ATP/8 photons or ATP/2.7 photon
