review lab table

8/22/2019 Review Lab Table

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Microbiology Final Review

Media Selective (chooseswhat will grow or

not grow; like the

salt in the agar

will chose what

will grow or not

grow)/differential (can

show you

different colors)

if something will

grow or not; may

grow different

colors; may

matabolize one of

the compounds

and turn a

different color)/

general (justwant it to grow,

dont know what

we have just want

it to grow)/

enriched

(provides

nutrients;

provides

something in the

agar that the

bacteria need to

grow)

Components that allow

for S/D/ETest for? pH or other

indicator

Examples of results Lab #

Trypticase soy

Agar (TSA)

GENERAL


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Blood Agar

(BAP)

ENRICHED The iron Hemolysis

(breaking down

RBC by endotoxin)

Alpha:

Green/YellowNOT clear

Beta: CLEAR/see

through/transparent

Gamma: didnt doanything, bacteria

just grew.

Staph and Strep

*Strep Pyogenese

Mannitol SaltAgar(MSA)

**Selectiveagent 7.5%Salt;

Differentialmannitol and

phenol red

Differential agent:mannitol & phenolred

Mannitol tests for:fermentation ofsugars

Phenol red testsfor: Ph

Phenol: pHindicatorlow pH -

yellow

neutral pH -

orangish

red

high pH -

pinkish red

-Staphylococcus aureus -yellow (lowpH) & (+) for

fermentation- Staphylococcus epidermis -

pink(highpH) & (-) forfermentation

Phenol Red

Broth (PRB)

Triple sugar

Iron (TSI)

SIM

(Sulfur [indole]

motility)

Endol?

If it can ferment

can carry outmetabolic

processes withoutoxygen

NEGATIVE: YELLOWISH

POSITIVE: BLACK


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MacConkey

(MAC)

Selective &

Differential

Bile Salts:

- selective

- supports gramnegative

Neutral Red:- differential- pH indicator

- low pH -

red/purple/pink colonies

- neutral -colorless

Lactose:

- differential- (LF) - lactose

fermentation

- (NLF) - nolactose

fermentation

Urea slant Looking to see

if they produce

urease

Yellow went to

hot pink if therewas presence

Citrate

Mueller-Hinton(MH)


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Other Tests: Test For/ How it works Positive Negative

Catalase (if the bacteria have

the enzyme present the bubbles)

Dont use for gram negative,just positive

With hydrogen peroxide;

bubbled on slide (production

of gas and bubbles)

BUBBLES if POSITIVE

Coagulase

Bacitracin/optochin

Kirby Bauer Put the discs to inhibit the

growth of bacteria

Looking for inhibition of

growth with the measure of

the diameters)

Is the inhibited part if it

inhibits it is is positive

Oxidase (use to look for

presence of cytochrome 3:

protein)

PAPER; turned purple/black

for positive

Enterotube:

**COMPOUND MICROSCOPE**

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