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RG/WP Poster presenters Wood protection

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RG/WP Poster presenters. Wood protection. Poster 398 Tomoyuki TAMENORI, Sakae HORISAWA. Genome-wide survey of cellulase related gene of white rot fungus, Pleurotus ostreatus. Kochi University of Technology Tomoyuki Tamenori 185 Miyanokuchi, Tosayamada, City Kami-city, Kochi JAPAN. - PowerPoint PPT Presentation

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Page 1: RG/WP Poster presenters

RG/WP Poster presenters

Wood protection

Page 2: RG/WP Poster presenters

Poster 398 Tomoyuki TAMENORI, Sakae HORISAWA

Kochi University of Technology

Tomoyuki Tamenori

185 Miyanokuchi, Tosayamada, City Kami-city, Kochi

JAPAN

Genome-wide survey of cellulase related gene of white rot fungus, Pleurotus ostreatus

Page 3: RG/WP Poster presenters

Poster 398 Genome-wide survey of cellulase related gene of white rot

fungus, Pleurotus ostreatus

Aim of work• Genes of Pleurotus ostreatus encoding

endocellulases were searched based on the information regarding the cellulases of other organisms.

Page 4: RG/WP Poster presenters

Poster 398 Results• Amino acid sequence data of cellulase (mainly

endoglucosidase) were obtained from the biological databases.

• Consensus-degenerate hybrid oligonucleotide primers (CODEHOP) were designed .

• PCR amplification and sequence analysis were conducted.

Page 5: RG/WP Poster presenters

Poster 398 Results

• PCR amplification was then performed using each pair of CODEHOPs.

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Sequencing