richard owen, 1843 defined concept of homologous organs
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Richard Owen, 1843 defined concept of homologous organs Homeotic transformations of organs in plants… “Metamorphosis of Plants” Published by Johan Goethe, 1790. - PowerPoint PPT PresentationTRANSCRIPT
Richard Owen, 1843 defined concept of homologous organs
Homeotic transformations of organs in plants…
“Metamorphosis of Plants”
Published by Johan Goethe, 1790
Wild type. APETALA1: PISTILLATA : AGAMOUS: "A" class "B" class "C" class
Wild type. APETALA1: PISTILLATA : AGAMOUS: "A" class "B" class "C" class
AP1 mRNA AG1 mRNA AP1 in AG1 mutant
~550 MYA
AGclade
Plena clade
plena
Shp1/2
AgFar
adaxial abaxial
apical
basal
Central zone
phyllotaxy
Abaxialdomain
Adaxialdomain
adaxial
abaxial
Lateral primordia formation/polarity:
abaxial identity:
crabsclaw, YABBY, KANADI - arabidopsis
adaxial identity:
phantastica - Antirrhinumroughsheath - maizephabulosa - arabidopsis
1. Ad/ab polarity is needed for lateral expansion
2. Adaxial cells determine initiation of the axial meristem
McConnell and Barton. 1998. Development 125:2935
PhbWild type
Wild Type phabulosa
STARTSterolbindingdomain
HomeoBox-ZipperDNA bindingdomain
Dominant Phabulosa/Phavulota mutations
WT
Phb
Emery et al. 2003. Current Biology, Vol. 13, 1768–1774,
Dominant Phb phenotype
Triple knockout phenotype:
Abaxialdomain
Adaxialdomain
Boundary required for Later growth of leaf blade
Development 126, 4117-4128 (1999)Members of the YABBYgene family specify abaxial cell fate in ArabidopsisKellee R. Siegfried1,*, Yuval Eshed1, Stuart F. Baum1, Denichiro Otsuga2, Gary N. Drews2and John L. Bowman1,‡
KANADI regulates organ polarityin ArabidopsisRandall A. Kerstetter, Krista Bollman, R. Alexandra Taylor,Kirsten Bomblies & R. Scott Poethig
2001 Nature 411:706-8
Abaxial determinants:
STARTSterolbindingdomain
HomeoBox-ZipperDNA bindingdomain
Dominant Phabulosa/Phavulota mutations
Carrington and Ambros 2003, Science 301:337
PTGS pathway in eukaryotes
Adapted from Williams and Rubin 2001, PNAS
Carpel Factory (DCL1)
Jacobsen et al. 1998. Development 126:5231
Argonaut phenotypes
AGO is a component of the RISC RNA induced silencing complex
Kidner and Martienssen, Nature. 2004 Mar 4;428(6978):81-4
miR165 WT
miR165 ago
PHB WT
PHBago
Abaxialdomain
Adaxialdomain
Boundary required for Later growth of leaf blade
Asymmetric expression of YAB3 requiresKANADI function
PHABULOSA expression in the radialized(adaxialized) KANADI triple mutant
In tomato, YABBY is restricted to cytoplasmic dense cells at the margins of the ad/ab boundary
Emery et al. 2003. Current Biology, Vol. 13, 1768–1774,
Bohmert et al EMBO J 17:170-180
Meristem initiation/maintenance:
Meristem size:
Lateral organ polarity:
clavata1clavata2clavata3
Abaxial genes:crabsclaw, YABBYKanadi
Adaxial genes:phabulosaPhavolutaRevoluta
Knotted -maizeshoot apical meristemless -arabidopsis
Organ number and polarity:Carpel FactoryArgonaute
STARTSterolbindingdomain
HomeoBox-ZipperDNA bindingdomain
Dominant Phabulosa/Phavulota mutations
Bohmert et al EMBO J 17:170-180
adaxial
abaxial
Lateral primordia formation/polarity:
abaxial identity:
crabsclaw, YABBY, KANADI - arabidopsis
adaxial identity:
phantastica - Antirrhinumroughsheath - maizephabulosa - arabidopsis
Carrington and Ambros 2003, Science 301:337
Wild type. APETALA1: PISTILLATA : AGAMOUS: "A" class "B" class "C" class
AP1 mRNA AG1 mRNA AP1 in AG1 mutant
STARTSterolbindingdomain
HomeoBox-ZipperDNA bindingdomain
Dominant Phabulosa/Phavulota mutations
PhvAdaxial domain
Domain boundary Required for lateralgrowth
WT clv3 clv3
Fletcher et al. 1999. Science 283:1911-14
KANADI expression in the abaxial domain
Development 126, 4117-4128 (1999)Members of the YABBYgene family specify abaxial cell fate in ArabidopsisKellee R. Siegfried1,*, Yuval Eshed1, Stuart F. Baum1, Denichiro Otsuga2, Gary N. Drews2and John L. Bowman1,‡
Development 126, 4117-4128 (1999)Members of the YABBYgene family specify abaxial cell fate in ArabidopsisKellee R. Siegfried1,*, Yuval Eshed1, Stuart F. Baum1, Denichiro Otsuga2, Gary N. Drews2and John L. Bowman1,‡
Establishment of polarity in lateral organs of plantsYuval Eshed*, Stuart F. Baum, John V. Perea and John L. Bowman
Current Biology 2001, 11:1251–1260