rna polymerase binding
DESCRIPTION
RNA Polymerase binding. *Constitutive or basal level transcription. Allosteric activation of RNA polymerase. Inhibition of transcription. DNA-protein interactions. Negative Control of the lac Operon. Proposed CAP-cAMP Activation of lac Transcription. Transcription in Eukaryotes. - PowerPoint PPT PresentationTRANSCRIPT
RNA Polymerase binding
*Constitutive or basal level transcription
Allosteric activation of RNA polymerase
Inhibition of transcription
DNA-protein interactions
Negative Control of the lac Operon
Proposed CAP-cAMP Activation of lac Transcription
S.R. Eddy (2001) Noncoding RNA genes and the modern RNA world, Nature Reviews Genetics, 2:919-929
Three major classes of RNA:
Transcription in Eukaryotes
Type Encodes Copies/genome % Total RNA
mRNA proteins Single or few 10%
tRNA functional RNA Few 20%
rRNA functional RNA Many 70%
But there are more!!!
Epitope Tagging of RNA Polymerase II
*Genetically “tag” Polymerase II subunit
*Grow cells in radioactively labeled amino acids
*Allow assembly of active polymerase
*Add antibody that binds epitope
*”Grab” antibody and what ever it is bound to
*SDS electrophoresis
Transcription*RNA polymerase II
Transcription*RNA polymerase II