root exudates involvement in tomato plant response to low
TRANSCRIPT
Root exudates involvement in tomato plant response to low P levelsV. Santoro1, M. Schiavon2, A. Ertani2, S. Nardi2, M. Martin1, D. Said-Pullicino1, L. Celi1
1 DISAFA, Università Degli Studi Di Torino, Grugliasco (TO), Italia; 2 DAFNAE, Università Degli Studi Di Padova, Legnaro (PD), Italia
Carotenoid-derived compounds (Fig. 1) with effects on rootgrowth and architecture (Fig. 2) and exudates production andcomposition
STRIGOLACTONES (SLs)
AIM OF THE WORK and HYPOTHESIS
PLANTS AND EXUDATES CHARACTERIZATION
1. P deficiency can induce several variations in exudate composition;
2. WT and KO plants reacted differently to the stress regimen in terms of quantity and quality of exuded compounds.
Preliminary aeroponic experiment
P deficiency can induce several variations in exudate composition
WT and SL- plants reacted differently to the stress regimen in terms of quantity
and quality of exuded compounds
Evaluation of the effect of exudates on P release from other Fe-P
adsorbed/coprecipitated systems with different P forms and P/Fe (Fig. 5)
CONCLUSIONS
EXPERIMENTAL DESIGN
Does SLs exudation influence the quality and quantity
of root exudates?
Fig. 2: SLs effects on: a) internode outgrowth, b) leaf senescence,c) root hair and primary root, d) secondary growth, e) axillary budoutgrowth, f) adventitious roots and g) lateral roots (Al-Babili et al.,2015; Yoneyama et al., 2007).
ROOT EXUDATES and P DEFICIENCY
P is an essential nutrient but often a growth limiting factor
Plants strategies to cope with P deficiency include the
exudation of organic acids, polyphenols, enzymes
P deficiency has also a stimulatory effect on the production
and exudation of SLs by tomato plant roots
INT
RO
DU
CT
ION
Fig. 1: Chemical structure of SLs
QUESTION
Understanding the role of SLs and root exudates in
tomato plants response to P stress conditions
The enhanced exudation of SLs may modify the chemical
composition of tomato root exudates in order to
improve P release and uptake from soil
Hydroponic experiment
Wild-type (WT) tomato plants (M82) and plants with gene for SLs synthesis
(CCD7) silenced (SL-) were left in P deficiency conditions for 10 days in an
aeroponic growth system (Fig. 3). Exudates were collected in 10 L solutions
according to the reported scheme and analysed.
T4 analyses revealed too diluted exudates!
COLLECTION SCHEME
Day Mon Wed Fry Mon Wed
Samplename T0 T1 T2 T3 T4
Fig. 3: aeroponic growth system
Water Change
Water Change
Water Change
Water Change
WT and SL- plants were sewed and germinated in peat pots, moved into 100 mL
flasks (hydroponic growth system, Fig. 4) and left with full nutrient solution for 10
days. Then P stress condition started (10 days).
Fig. 4: hydroponic growth system
• Biomass parameters• Total P content• Carbon (C) and Nitrogen (N) content• Chlorophyll content (SPAD index)• Strigolactones• Organic Acids• Polyphenols• Hormonal activity• Inorganic and organic P content• Total P content• Dissolved Organic Carbon (DOC) and
Nitrogen (DON)
PL
AN
TS
EX
UD
AT
ES
WT + P WT - P SL- + P SL- - PPlanttype
Pi(μg)
Porg
(μg)
WT + P 3.18 45.95
WT - P 1.41 37.05
SL- + P 2.66 35.68
SL- - P 2.17 36.17
0
5
10
15
20
25
30
35
40
pp
m
Oxalic acid
WT + P WT - P KO + P KO - P
a
b
ab
b
Plants
TREATMENTS
WT+P(3 plants)
WT-P(3 plants)
SL- +P(3 plants)
SL- -P(3 plants)
Exudates
Marked decrease in both roots and shoots
biomass of WT plants, with an opposite
trend in the SL-. Total P and water contents
have the same trend as biomass, and WT
plants increase their PUE (phosphorus use
efficiency) under P deprivation. In general, a
stronger effect of P deprivation is observed
in WT plants
ORGANIC ACIDS
Greater production of oxalic acid
with P depletion in WT plants.
Succinic acid present in high
concentration (40-60 ppm) but not
influenced by P depletion. Citric,
malic, glycolic, lactic, acetic present
in concentrations <1 ppm
Gallic acid was quantified in an
intermediate sampling time in the
aeroponic system
Fig. 5: Fe-Pcoprecipitated system
PHOSPHORUS
POLYPHENOLS
BibliographyAl-Babili, S., Bouwmeester, H.J., 2015. Ann Rev Plant Biol, pp. 161-186. Santoro et al., 2019 (Geoderma, in press)Yoneyama, K., Xie, X., Kisugi, T., Nomura, T., Sekimoto, H., Yokota, T., 2011. Plant Growth Regul, 65(3), 495-504.
0
2
4
6
8
10
WT+P WT-P KO+P KO-P
FW
bio
mas
s(g
)/P
co
nte
nt
(mg
)
PUE
SL- +P SL- -P
-0,40
-0,20
0,00
0,20
0,40
0,60
0,80
1,00
WT+P WT-P KO+P KO-P
P (m
g/p
lan
t)
P content
Roots ShootsSL- +P SL- -P
EFFECT OF EXUDATES ON P AND Fe RELEASE
0,00
0,10
0,20
0,30
0,40
0,50
0,60
0,70
0,80
0,90
1,00
0 50 100 150 200 250 300 350
M/M
0
TIME (MIN)
Citric 10 mM Ascorbic 10 mM
Oxalic 10 mM Gallic 10 mM
Gallic 5 mM + Citric 5 mM
0,00
0,10
0,20
0,30
0,40
0,50
0,60
0,70
0,80
0,90
1,00
0 50 100 150 200 250 300 350
M/M
0
TIME (MIN)
Dissolution rate: oxalic > ascorbic > citric > citric + gallic > gallic
Some of the compounds characterized in the exudates(polyphenols and organic acids) were used to dissolve a Fe-Psystem obtained through oxidative precipitation of Fe(II) in thepresence of inorganic P (P/Fe ratio = 0.5) (Santoro et al., 2019),thanks to their reducing or chelating ability
P
Fe
FUTURE WORK
This research has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 727929 (A novel and integrated approach to increase multiple and combined stress tolerance in plants using tomato as a model - TOMRES)
SL- +P SL- -PWT+P WT-P