Hot tnh sinh hc ca cy cc kn

Video m uhttps://youtu.be/RLM6n_o2fcA

Hot tnh sinh hc ca rotenone trong cy cc knTRNG I HC BCH KHOA-I HC QUC GIA TP.H CH MINHKHOA K THUT HA HC-B MN CNG NGH SINH HC ti liu lunGVHD: Ths.Phan Th Thanh NgaSVTH: Nguyn c Trung MSSV : 61204140Phn ng dng ly trong SFC2112Cu trc bi thuyt trnh1. Gii thiu s lc v i tng2. Hp cht ha hc trong cc kn3. C ch tc ng4. ng dng- Thu hp cht5. Ti liu tham kho6. Tm tt

1.Gii thiu v cc knB: FabalesH: FabaceaeChi: DerrisLoi: Derris trifoliata

B sung v hnh nh, ngnh lp b h ging chi loi42.Hp cht ha hcGii thiu v hp cht ha hcCTPT: C23H22O6 thuc nhm iso-flavonoidM=394,42 g/molNhit nng chy: 165-166oCNhit si: 210-220 oCT trng: 1.27g/cm3

ROTENONE ha tan: ha tan rt hn ch trong nc 0,2 mg / L @ 20 o C, 15 mg / L @ 100 o C; ha tan trong hu ht cc dung mi hu c bao gm ethanol 2g / L @ 20 o C; carbon tetrachloride 6 g / L @ 20 o C; amyl acetate 16 g / L @ 20 o C; xylene 34 g / L @ 20 o C; acetone 66 g / L @ 20 o C; benzen 80 g / L @ 20 o C; chlorobenzene 135 g / L @ 20 o C; ethylene dichloride 330 g / L @ 20 o C; chloroform 472 g / L @ 20 o C5iu ch rotenone t iso-flavonoid

ROTENONEHydroxyl haMetyl ha3. C ch tc ngRotenone tc ng vo chui vn chuyn in t trong ty th

Chui chuyn in t kt hp s chuyn gia vt cho in t (v d nh NADH) v mt vt nhn in t (v d xi) n s trung chuyn ca proton H+ qua lp mng sinh cht. Kt qu l mt chnh th in ha hc c to ra, nng lng tch cha trong th ny c ATP synthase chuyn thnh ha nng di dng adenosine triphosphate (ATP). Chui chuyn in t l mt c ch cp t bo v c dng sn sinh nng lng cho t bo.

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c ch NADH- ubiquinone reductase C ch tc dng

Rotenone hot ng bng cch can thip vo chui vn chuyn in t trong ty th. c th, n c ch s chuyn electron t cc trung tm st-lu hunh trong phc hp I ubiquinone.iu ny gy tr ngi vi NADH trong qu trnh to nng lng t bo s dng c (ATP).Oxy Cellular c gim n mc cc oan, to ra mt Oxygen Species Reactive, m c th gy tn hi DNA v cc thnh phn khc ca mitochondria

8Video v phc hp 1https://youtu.be/GF_wIxKoS_U

Nhiu kh nng rotenone lin kt cht ch vi cc trang web ubiquinone-rng buc ca cc enzyme ngn chn li vo ca mt cht nnc ch cnh tranh10

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Trong cc h thng nc t nhin, t l hy dit ca rotenone bng cch thy phn, qu trnh oxy ha, v chuyn i quang ha thnh cc hp cht v cc mnh v ca c tnh thp hn l ph thuc vo mt s yu t nh nhit , pH, cng ca nc, v nh sng mt tri. Trong 'in hnh' t nhin h thng ao, sui, mt na cuc sng ca rotenone trong nc t nhin dao ng t khong mt na ngy 24 C (75 F) n 3,5 ngy 0 C (32 F)12

4. NG DNG- THU HP CHTSn xut bng cch chit xut t thn hoc r ca cc chi Lonchocarpus v DerrisS dng nh thuc tr su hay thuc dit cc s dng rng ri M ,hn 20 tn mi nm

Hnh minh ha14

Phn tn, pha trn dch nh tng cha rotenone vo ncRi u trn b mt ao nuiSau 10p c bt u chtThu gom cPhn hy rotenoneX l li nc ao nuiBt u th cThuc dit c165. Ti liu tham kho

[1] Rotenone.a review of its toxicity and use for fisheriesmanagement ,SCIENCE FOR CONSERVATION 211 ,Nicholas Ling[2] INTERNATIONAL JOURNAL OF PHARMACY & LIFE SCIENCES,Diuretic activity and brine shrimp toxicity of Derris trifoliata Lour.Saifullah Al Mamoon1 and Md. Golam Azam2,3*[3] Antimicrobial Activity of Bioacive Metabolites Isolated from Selected Medicinal Plants[4] http://www.qub.ac.uk/schools/SchoolofBiologicalSciences/People/DrAGalkin/Bioenergetics/AllaroundBioenergetics/6. Tng kt

Matrix

H+ + NADH NAD+ + 2H+ 2H+ + O2 H2O

2 e

I Q III IV Intermembrane Space

cyt c