7/31/2019 Saccharomyces Cerevisiae Methane

1/33

Z`jja`}fc~jgz jg}gtnzn`gNh }gb`{nfh {fcg{a`hg cn{no`{nfh nh }wcnh`h{z

Zug`ig}< @}wcm`i` Zwkagg} M`mw"

J}gkn{ zgcnh`} fh

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2/33

Nh{}fkwj{nfh Fw{ fd {ag {f{`b obfm`b o}ggh afwzg gddgj{z j`wzgk m~ {ag OA o`zgz"

40' nz `jjfwh{gk df} m~ JF=" 0>' m~ JA; `hk 4' m~ H=F(

Jfh{}nmw{nfh fd knddg}gh{ jfwh{}ngz {f {f{`b OAOz(

Jfwh{}~ !'. [f{`bJanh` 0?Whn{gk Z{`{g 04

Gw}fug`h whnfh 00

Nhkfhgzn` 4

Nhkn` 7

]wzzn` 7

M}`|nb ;

I~f{f u}f{fjfb =55?

7/31/2019 Saccharomyces Cerevisiae Methane

3/33

Bntgz{fji ufuwb`{nfh !nh cnbbnfhz. fd Nhkn` D@F"=554.

Zugjngz Ufuwb`{nfh

J`{{bg ==4(0

Mwdd`bfgz >4(>Zaggu 7>

Of`{ 0=;(>

Unoz 01(7

J`cgbz 5(>

Kfhig~z 5(>^`iz" cn{awhz" cwbgz g{j @uu}fq( 25(>

@mftg ufuwb`{nfh `jjfwh{z df} 00(>7' fd {ag obfm b bntgz{fji ufuwb`{nfh( U}fkwjnho 0=(;7' fd{ag {f{`b gh{g}nj JA; gcnzznfhz

I`c}` g{ `b("=505

fh{}nmw{nfh fd Nhkn`h bntgz{fji df} cg{a`hg u}fkwj{nfh `z }guf}{gk m~ t`}nfwz f}ig}z]gdg}ghjg ]guf}{gk t`bwg ![o& g`}.

Ia`h !0>>4. 1(>

Znhoa !0>>1. >(5=:

O`}o `hk Zawib` !=55:. ?(=7?

Znhoa`b g{ `b !=557. 05(51

7/31/2019 Saccharomyces Cerevisiae Methane

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0( Obfm`b `}cnho

=( F|fhg b`~g} kgubg{nfh

Ughg{}`{nfh fd WT }`~z {f {ag

g`}{az `{cfzuag}g

Zinh j`hjg}Ncu`n}gk nccwhg z~z{gc]g{nh`b kgoghg}`{nfh

NUJJ =55?

A`}cdwb gddgj{z fd anoa jfhjgh{}`{nfh fd cg{a`hg nh `{cfzuag}g

[ff cwja fd N] }`kn`{nfh {}`uugk j`wznhoo}ggh afwzg gddgj{

@tg}`og obfm`b {gcug}`{w}gz nbb }nzg m~ {ag ~g`} =5:5"

f}bk nz bnigb~ {f mg 0= J `}cg} {a`h {fk`~(

7/31/2019 Saccharomyces Cerevisiae Methane

5/33

O`zgz 0?75 0>>1 =557

JF= =>= uuc :44 uuc :?> uuc

JA; ?=> uum 0?4: uum 0??; uum

H=F =?7 uum :0; uum :0> uum

NUJJ =55?

Ja`hogz nh Jfhjgh{}`{nfh fd knddg}gh{ OAOz df}

{ag b`z{ :55 ~g`}z(

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Cg{a`hg jfh{}nmw{nfh {a}fwoa t`}nfwz zfw}jgz fd `o}njwb{w}g zgj{f}

@o}njwb{w}g zgj{f} JA; gcnzznfh !'.

Gh{g}nj dg}cgh{`{nfh 4:

]njg jwb{nt`{nfh =0

J}fu zfnbz 0:

C`hw}g c`h`ogcgh{ =(?

NHJJ@ =505

7/31/2019 Saccharomyces Cerevisiae Methane

7/33

7/31/2019 Saccharomyces Cerevisiae Methane

8/33

Z{}`{gongz {f }gkwjg Cg{a`hg Gcnzznfh

C`h`ogcgh{ z{}`{gongzHw{}n{nfh`bz{}`{gongz

J`}mfa~k}`{gz

D}gpwghj~ fd dggknho

Df}`og zugjngz `hk n{zc`{w}n{~

Jfcubg{g dggkmbfji&[C]

U}fjgzznho fd df}`og

Znb`og dggknho

C`hnuwb`{nfh fd }wcghdg}cgh{`{nfh

0( Nfhfuaf}gz=( Kgd`wh`{nfh

:( @kkn{nfh fd d`{ $ fnbz;( KDC

7( U}funfh`{g gha`hjg}z4( Ub`h{ =5 jfcufwhkz?( Nccwhn|`{nfh1( Wzg fd jagcnj`bz

>( ]gkwj{ntg jg{foghgznz05( U}gmnf{njz00( Gbgj{}fh `jjgu{f}z0=( ]wcgh cg{a`hg

fqnk`{nfh0:( M`j{g}nfjnhz

0;( M`j{g}nua`ogz

Hwcmg} `hku}fkwj{ntn{~ fd

`hnc`bz

Oghg{nj zgbgj{nfhfd `hnc`bz

7/31/2019 Saccharomyces Cerevisiae Methane

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Cnj}ff}o`hnzcz wzgk `z KDC df} }wcnh`h{z

Oghwz Zugjngz

B`j{nj `jnk u}fkwjnho m`j{g}n`

B`j{fm`jnbbwz B(`jnkfuanbwz" B(ub`h{`}wc" B(j`zgn" B(o`bbnh`}nwc"B(z`bnt`}nwz" B(}gw{g}n" B(mwbo`}njwz

Mndnkfm`j{g}nwc M(uzwgkfbfhowc" M({ag}cfuanbnwc" M(bfhowc" M(b`j{nz

Z{}gu{fjfjjwz Z(mftnz" Z(d`gjnwc

Gh{g}fjfjjwz G(d`gjnwc" G(d`gj`bnzB`j{nj `jnk w{nbn|nho m`j{g}n`

Cgo`zua`g}` C(gbzkghnn

U}funfhnm`j{g}nwc U(zagc`hnn" U(d}gwkgh}gnjann" U(`jnknu}funfhjn" U(lghzghnn

F{ag} m`j{g}n`U}gtf{gbb` U(m}~`h{nn

M`jnbbwz M(zwm{nbnz" M(bnjaghnd}fcnz" M(jf`owb`hz

^g`z{ Z`jja`}fc~jgz jg}gtnzn`g" Z(mfwb`}knnDwhon" @zug}onbbwz @(f}~|`g" @(hnog}

Zgf g{ `b( !=505.

7/31/2019 Saccharomyces Cerevisiae Methane

10/33

Whnjgbbwb`} Dwhon

]gznz{`h{# {f `h{nmnf{njz" `h{n#m`j{g}n`b `ogh{z }gznz{`hjg nz

h`{w}`b `hk oghg{nj`b" `hk hf{ zwzjgu{nmbg {f mg cfkndngk f}

{}`hzcn{{gk {f f{ag} cnj}f#f}o`hnzcz(

Z`jja`}fc~jgz jg}gtnzn`g#Nhkwz{}n`bb~ ncuf}{`h{ `mnbn{~ {f jfhtg}{

zwo`}z !n(g( obwjfzg" c`b{fzg. nh{f g{a`hfb `hk j`}mfh knfqnkg

!m`inho" m}gnho" knz{nbbg}~" bnpwnk dwgb nhkwz{}ngz.

N{ nz a`tnho O]@Z z{`{wz !Oghg}`bb~ ]gjfohnzgk @z Z`dg. d}fc {ag

WZ Dffk `hk K}wo @kcnhnz{}`{nfh(

@wjb`n}" =55005

7/31/2019 Saccharomyces Cerevisiae Methane

11/33

Ua~znfbfonj`b z{`{g fdZ`jja`}fc~jgz jg}gtnzn`g

{a}fwoa knogz{ntg {}`j{

Z`jja`}fc~jgz jg}gtnzn`g }gc`nhz `bntg `cfho {ag knogz{ntg {}`j{" mw{

j`hhf{ jfbfhn|g(

Knz`uug`}gk }`unkb~ d}fc {ag o`z{}f#nh{gz{nh`b {}`j{ `{ jgzz`{nfh fd

kfznho(

!@wjb`n}" =550.

00

7/31/2019 Saccharomyces Cerevisiae Methane

12/33

^g`z{ zwuubgcgh{`{nfh

F= wu{`ig Cnj}fhw{}ngh{ zwuub~!Ugu{nkgz" tn{`cnhz" c`b`{g. B`j{nj @jnk cg{`mfbnzc

]gkfq uf{gh{n`b [f{`b m`j{g}n`" jgbbwbfb~{nj m`j{g}n`

]`{g fd FC knogz{nfh

uA Z{`mnbn|`{nfh

Ncu}ftgk hnc`b u}fkwj{ntn{~

Dggk nh{`igGhg}o~ `hk `cnhf `jnk zwuub~

]nzi fd `jnkfznz

Ag`b{a knzf}kg}

!Lfw`h~" =554.

7/31/2019 Saccharomyces Cerevisiae Methane

13/33

!Ja`wjag~}`z#Kw}`hk" =551.

Cgja`hnzcz fd `j{nfh fd bntg ~g`z{ u}fmnf{njz fh }wcnh`b dnmg}#kgo}`knho jfccwhn{ngz(

7/31/2019 Saccharomyces Cerevisiae Methane

14/33

^g`z{ }gkwjgcg{a`hg m~; cg{afkz

0( Kgj}g`zg hwcmg} fdU}f{f|f`!Hgmfbk g{ `b(" 0>>1.

=( U}fcf{g`jg{foghgznz!Ja`wjag~}`z g{`b( 0>>7.

:( Ncu}ftg `hnc`bu}fkwj{ntn{~!M}whf g{ `b(" =557.

;( Nhj}g`zgu}funfh`{gu}fkwj{nfh!Bnb` g{ `b(" =55;.

7/31/2019 Saccharomyces Cerevisiae Methane

15/33

Hgmfbk g{ `b(" 0>>1

7/31/2019 Saccharomyces Cerevisiae Methane

16/33

Ja`wjag~}`z g{ `b(" 0>>7

Nh Tn{}f A= w{nbn|`{nfh" cg{a`hg $ `jg{`{g u}fkwj{nfhZg}#1 @jg{foghnj z{}`nh" CD= cg{a`hfoghnj z{}`nh" bntg f} `w{fjb`tgk Z( jg}gtnzn`g z{}`nh fdJhJc#N#05?? E 051 jgbbz&cb9 nhjwm`{nfh {ncg 7 k`~z9

7/31/2019 Saccharomyces Cerevisiae Methane

17/33

Gddgj{z fd [nh#Z{}`nh 1;0? `hk 05=4 !^Z[. Z`jja`}fc~jgzjg}gtnzn`g bntg jgbbz !7 q 05> bntg f}o`hnzcz &o. fh nh tn{}fdg}cgh{`{nfh !h307.

N{gc ^Z[ o&B

5 5(:: 5(44 5(>> 0(:=uA 4(=: 4(=: 4(=; 4(== 4(=;

@ccfhn`#H" co&kB 0(=1 0(=0 0(=0 0(:= 0(=?

[f{`b TD@" cC 04(= 04(= 04(? 04(1 0?(;cfb&055 cfb

@jg{`{g 41(> 41(: 44(? 47(: 4;(>

U}funfh`{g 07(4 04(= 0?(5 0?(1 0?(>

@jg{`{g< U}funfh`{g ;(;= ;(== :(>0 :(41 :(4:

U}f{f|f`"q05; &cB =(: =(= =(; =(7 =(?

[f{`b o`z " cB 0(;? 0(;4 0(7= 0(45 0(??

A=" cC HK HK HK HK HK

JA;"cC 5(?0 5(?0 5(?0 5(?= 5(?=

4 a 5(=1 5(=? 5(=1 5(=4 5(=4

=; a 0(0; 0(07 0(0; 0(0? 0(0?

Gqu

7/31/2019 Saccharomyces Cerevisiae Methane

18/33

N{gc ^Z[ o&B

5 5(:: 5(44 5(>> 0(:=uA 4(5? 4(5? 4(54 4(57 4(5:

@ccfhn`#H" co&kB 7(= 7(5 7(= 7(: 7(=

[f{`b TD@" cC 71(; 40(= 4=(4 4;(5 47(:

cfb&055 cfb

@jg{`{g 7?(= 7?(0 7?(: 7?(0 7?(0

U}funfh`{g =4(; =4(1 =?(5 =?(; =?(1

@jg{`{g< U}funfh`{g =(01 =(0; =(0= =(51 =(54U}f{f|f`"q05; &cB 07(7 07(: 07(4 07(= 07(;

[f{`b o`z " cB =1(5 :0(0 :7(5 :?(7 ;5(5

A=" cC 5(01 5(0? 5(01 5(01 5(01

JA;"cC 0;(7 0;(4 0;(; 0;(; 0;(4

4 a 07(? 07(> 07(; 07(4 07(>

=; a 0:(: 0:(= 0:(: 0:(= 0:(;

[f{`b tn`mbg m`j{g}n`" q051&cB `{ =; a}

:(=7 ;(00 ;(;4 ;(7? ;(1;

B#b`j{`{g" cC `{ =; a} 5(=1 5(=0 5(01 5(0; 5(5>

Gqu

7/31/2019 Saccharomyces Cerevisiae Methane

19/33

N{gc ^Z[ o&B

5 5(:: 5(44 5(>> 0(:=uA 7(1: 7(1= 7(15 7(?> 7(?1

@ccfhn`#H" co&kB ?(? ?(4 ?(1 ?(1 ?(?

[f{`b TD@" cC 70(: 7;(: 77(4 7?(= 71(;

cfb&055 cfb

@jg{`{g 71(? 71(? 71(7 71(7 71(1

U}funfh`{g =7(7 =4(7 =4(> =?(4 =1(5

@jg{`{g< U}funfh`{g =(:5 =(== =(01 =(0: =(05U}f{f|f`"q05; &cB 0:(; 0:(= 0:(4 0:(: 0:(7

[f{`b o`z " cB :0(5 :;(4 :?(7 :>(5 ;0(0

A=" cB&nhjwm`{nfh 5(00 5(00 5(05 5(00 5(05

JA;"cB&nhjwm`{nfh 0=(5 0=(0 0=(5 00(1 0=(0

4 a 0=(; 0=(4 0=(7 0=(: 0=(4

=; a 00(45 00(7; 00(;= 00(=4 00(4:

[f{`b tn`mbg m`j{g}n`" q051&cB `{ =; a}

=(51 :(0; :(74 :(?: :(11

B#b`j{`{g" cC `{ =; a} 01(?5 04(:: 07(?4 0;(:; 0:(0=

Gqu

7/31/2019 Saccharomyces Cerevisiae Methane

20/33

N{gc ^Z[ o&B

5 5(:: 5(44 5(>> 0(:=uA 4(== 4(=5 4(01 4(0> 4(=0

@ccfhn`#H" co&kB 07(4 07(; 07(; 07(; 07(4

[f{`b TD@" cC ?:(> ?4(1 ??(> 15(4 1=(:

cfb&055 cfb

@jg{`{g 45(= 45(; 45(? 45(> 40(=

U}funfh`{g =;(: =;(? =;(> =7(0 =7(=

@jg{`{g< U}funfh`{g =(;> =(;7 =(;; =(=7 =(0>U}f{f|f`"q05; &cB 0=(7 0=(; 0=(1 0=(; 0=(7

[f{`b o`z " cB =>(7 :4(7 :1(7 ;0(7 ;:(5

A=" cB&nhjwm`{nfh 5(01 5(0? 5(01 5(01 5(01

JA;"cB&nhjwm`{nfh 0?(0 04(7 04(; 04(: 04(:

4 a 0?(? 0?(? 0?(? 0?(? 0?(1

=; a 04(; 07(; 07(0 0;(1 0;(1

NTKKC" ' =;a} ;7(5 ;4(0 ;?(; ;1(1 ;>(?

[f{`b tn`mbg m`j{g}n`" q051&cB `{ =; a}

4(>1 ?(;4 ?(?: ?(>1 1(;;

Jgbbwbfb~{nj m`j{("q054&cB ;(?? 7(:; 7(10 7(>? 4(;>

B#b`j{`{g" cC `{ =; a} 5(0> 5(00 5(5> 5(54 5(5;

Gqu

7/31/2019 Saccharomyces Cerevisiae Methane

21/33

Gddgj{z fd = `j{ntg k}ngk ~g`z{ z{}`nhz fh }wcnh`b `jnkfznz hk cg{a`hgu}fkwj{nfh nh hfhb`j{`{nho Afbz{gnh jfz

U`}`cg{g} [}g`{cgh{Jfh{}fb Z{}`nh 0 Z{}`nh =

KCN io&k`~ 0;(0 0:(> 07(=

Nh{n`b M(_ !io. ?17 1=7 ?>?

Dnh`b M(_ !io. 1== 17= 1:=

Ja`hog nh M_!io. :? =? :7

Jawho g{ `b(" =500

h307

U`}`cg{g}[}g`{cgh{

Jfh{}fb Z{}`nh 0 Z{}`nh =

KC"' 4=(> 40(? 40(=

FC"' 4:(1 4=(? 4=(0

JU"' 4:(4` 40(=m 4=(;`m

HKD"' ;5(5 :1(: :?(?

@KD"' =;(4 =;(0 =:(;

Knogz{nmbg KCN" io&k`~ >(5 1(7 >(5

^g`z{ `kkgk tn` }wcgh j`hwb` E0q0505 JDW&ag`k&k`~" dgk [C] df} :7 k`~z

7/31/2019 Saccharomyces Cerevisiae Methane

22/33

U`}`cg{g}

[}g`{cgh{

Jfh{}fb Z{}`nh 0 Z{}`nh =

Cg`h ]wcnh`b uA 4(=;` 4(:;` 7(>1m

[f{`b U}f{f|f`"q054 jgbbz &cB 0(7 0(1 0(1

[f{`b TD@" cC 000 004 0=5

Nhkntnkw`b TD@" cfb&055 cfb

@jg{`{g 4:(5`m 4:(:` 40(;m

U}funfh`{g 0>(5m 01(:m =5(;`

Mw{~}`{g 0:(: 0:(7 0:(?

@

7/31/2019 Saccharomyces Cerevisiae Methane

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U`}`cg{g}

[}g`{cgh{

Jfh{}fb Z{}`nh 0 Z{}`nh =

Cg{a`hg {f{`b gcnzznfh" o&k =41 =40 =4:

KCN kw}nho JA; jfbbgj{nfh"io&k 04(0 07(0 04(?

Cg{a`hg gcnzznfh nh{ghzn{~

]`{nf" o fd JA;&io fd KCN 04(>`m 0?(7` 07(?m

Ug}jgh{`og fd o}fzz ghg}o~nh{`ig

7(4`m 7(1` 7(=m

Jawho g{ `b(" =500

7/31/2019 Saccharomyces Cerevisiae Methane

24/33

U}fmnf{nj NTKCK!'.

[TD@!cgp&

055cb.

@ccfhn` H!co&

055cb.

[f{`b O`zU}fkwj{nfh

!cb&o wmz{}`{g.

JA;!cb&055 oFC knogz{gk

M`j{g}n`bjfwh{z Hfz&cb.

Jfh{}fb ;0(>> 4(:? 00(=? 7?(75 07(4: 7(:051

Z(J :> ;0(4= 4(0: 05(4? 74(4? 07(7: 7(? 051

Z(J ;5 ;=(?0 ?(5? 05(4: 74(?5 0;(45 7(= 051

Z(J ;0 ;=(70 4(?5 05(:5 71(7? 0;(0? 7(4 051Z(J ;= ;0(;: 4(;? 05(;: 7?(?5 0;(1? 4(5 051

Z(J ;: ;=(44 4(7: 00(;? 7?(:5 0;(0? 4(= 051Z(J ;7 ;:(?? ?(;: >(?5 7>(4: 0=(45 4(: 051Z(J ;4 ;0(74 4(4? 05(5: 77(75 0:(7: 7(4 051Z(J ;? ;=(54 4(7? >(4: 71(0? 07(=: 4(0 05

1

Z(J 75 ;4(=5 ?(:? >(0: 4=(:? 0=(1? 4(: 051Z(J 014 ;0(71 4(45 >(;? 40(5: 0;(=: 4(: 051Z(J 01> ;4(51 ?(?? >(7: 40(=: 0;(55 4(4 051Z(J ==7 ;?(04 ?(4: >(4? 4=(15 0:(=: 4(4 051

Gddgj{ fd Z`jja`}fc~jgz jg}gtnzn`g !Z(J." zwuubgcgh{`{nfh fh {ag Nh tn{}f }wcgh dg}cgh{`{nfh

]`c`h C`bni $ ]`cgzag} Znhoa" =55>

0= jwb{w}gz fd ZJ uw{ nh E 05> JDW&jfhnj`b db`zi $ ]wcgh bnpwf} d}fc kwb{ mwdd`bf

7/31/2019 Saccharomyces Cerevisiae Methane

25/33

N{gc [}g`{cgh{

K}~ c`{{g} kgo}`k`mnbn{~ !'. ` !'. m !'. j !'.

: a 4 a 0= a =; a

K}ngk mgg} # :=(4 ::(0 74(1 15(: 00(7 11(4 4(=

@tg}`og Hf`kkn{nfh

0?(5 0?(5 =:(4 =1(5 0;(1 17(= 5(1

^g`z{ 04(4 0>(7, =>(1, :1(>,, 0=(> 1?(0 0(4,,

@tg}`og Hf`kkn{nfh

0:(: 0?(? =7(; :7(5 05(4 1>(; 0(=

^g`z{ 04(0 0>(:, =1(1 ;5(? 00(7 11(7 0(4@tg}`og Hf

`kkn{nfh05(1 07(: ==(1 ;=(4 :(5 >?(5 =(=

KH@ 0;(1 =0(1,, =>(0,, ;7(7, >(0, >0(5, =(=@tg}`og Hf

`kkn{nfh05(1 07(: ==(1 ;=(4 :(5 >?(5 =(=

@bmwcnh 07(4 =5(;,, =>(1 ;7(4 >(4,, >5(;,, =(=@hkf g{ `b(" =55:

Gddgj{ fd K}ngk Mgg} ^g`z{ fh {ag Nh tn{}f }fwoa`og kgo}`k`mnbn{~ `hk cg{a`hg u}fkwj{nfh

,u25(579 ,,U25(50

Gddgj{ fd k}ngk mgg} ~g`z{ fh {ag kgo}`k`mnbn{~ fd }fwoa`ogz

Kgo}`k`{nfh ja`}`j{g}nz{njz fd k}ngk mgg} ~g`z{

Gddgj{ fd k}ngk mgg} ~g`z{ gq{}`j{" KH@ hk @bmwcnh fh {ag kgo}`k`mnbn{~ fd }fwoa`ogz

_afbg j}fu jf}h" }njg z{}` hk N{`bn`h }~go}`zz

7/31/2019 Saccharomyces Cerevisiae Methane

26/33

N{gc [}g`{cgh{ K}~ c`{{g} kgo}`k`mnbn{~ !'.

: a 4 a 0= a =; a

@tg}`og ^g`z{ #5(;` =(;` 4(= 05(>`

^g`z{ Gq{}`j{ =(=`m =(=` =(1 4(;`

KH@ ;(5m 4(;m 4(: :(5m

@bmwcnh ;(1m

7(0m

4(; :(5m

@hkf g{ `b(" =55:

Knddg}ghjg mg{ggh hf `kkn{nfh `hk `kkgk z`cubgz

Nhjwm`{nfh{ncg

[}g`{cgh{

Jfh{}fb Mgg}~g`z{

0= a 0;(0 ;;(0,,

=; a =1(; 45(=,,

Nh tn{}f cg{a`hg u}fkwj{nfh

^g`z{ gq{}`j{ nz gpwnt`bgh{ {f 5(=o fd k}ngk mgg} ~g`z{KH@ kkgk `z 5(554o anja ontgz hwjbgnj jnk jfh{gh{ gpwnt`bgh{ {f 5(=o k}ngk mgg} ~g`z{@bmwcnh `kkgk `z 5(5>>; o anja ontgz ` JU jfh{gh{ gpwnt`bgh{ {f 5(=o k}ngk mgg} ~g`z{

,u25(579 ,,U25(50

@k bnmn{wc k}~ c`{{g} nh{`ig `hk }wcnh`b dg}cgh{`{nfh t`}n`mbgz df}

7/31/2019 Saccharomyces Cerevisiae Methane

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