8/2/2019 sb bld 430 quiz 5 key

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BLD 430 Quiz 5

1. Agar is usually supplied as a dried powder and the gel is prepared to certain percentage(w/v).

A) True

B) False

2. Acrylamide is usually supplied as a liquid stock solution and diluted to a certain

percentage (v/v).A) True

B) False

3.Nucleic acid gels may be stained with either ethidium bromide or sybrgreen but eitherwill require UV light to visualize.

A) True

B) False

4.Nucleic acid gels can be silver stained, just like a protein gel.A) True

B) False

5. Once stained, a gel can be documented using transillumination with standard or UVlight depending on the staining protocol.

A) True

B) False

6. Capillary electrophoresis can be have very high current applied because of the small

amount of matrix used.A) True

B) False

7. Capillary electrophoresis uses an acrylamide matrix but it is not polymerized.A) True

B) False

8. Pulsed field electrophoresis apply current in alternating paths to allow large nucleicacid fragments to migrate without shearing.

A) True

B) False

9. Pulsed field electrophoresis is used for epidemiological studies in areas such as food

borne pathogens.A) True

B) False

8/2/2019 sb bld 430 quiz 5 key

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10.Nucleic acid strands that are smaller will not migrate very far from the origin while

larger molecules will move rapidly from the origin.

A) TrueB) False