scheme syllb(3-8th sem)biotech bembacorrected

Panjab University

Scheme and Syllabus ofB.E. and B.E. MBA

B.E. (Biotechnology)3rd to 8th semester

B.E. MBA (Biotechnology)3rd to 6th Semester

2010-2011

SCHEME OF EXAMINATION OF B.E. BIOTECHNOLOGY

Second Year - Third Semester

Theory PracticalTheory Paper Code

Paper Title Hours/Week

MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.

Practical Paper Code

BIO301 Concepts in Bio-Technology-I 4+0 100 50 2 50 50 BIO351BIO302 Microbiology 4+0 100 50 3 50 50 BIO352BIO303 Biochemistry 4+0 100 50 3 50 50 BIO353BIO304 Cell & Molecular Biology 4+0 100 50 2 50 50 BIO354BIO305 Material and Energy Balance

Calculations3+1 100 50 -- -- -- --

BIO306

WS-357

Mathematics-III

Workshop Practice of 4 weeks after 2nd semester exams:

3+1 100 50 -- -- --

50

--

Grand Total: 1350 24 600 300 10 200 250

Second Year - Fourth Semester



MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.


BIO401 Concepts in Biotechnology-II 4+0 100 50 2 50 50 BIO451BIO402 Process Thermodynamics 4+0 100 50BIO403 Chemical Reaction

Engineering4+0 100 50 2 50 50 BIO453

BIO404 Industrial Microbiology 4+0 100 50 2 50 50 BIO454BIO405 Immunology & Immuno-

technology4+0 100 50 2 50 50 BIO455

BIO406 Legal Laws and IPR 4+0 100 50 -- -- -- --Grand Total: 1300 24 600 300 8 200 200

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Third Year - Fifth Semester



MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.


BIO501 Enzyme Engineering & Technology

4+0 100 50 2 50 50 BIO551

BIO502 Bio-Process Engineering 4+0 100 50 2 50 50 BIO552BIO503 Animal Cell Culture &

Biotechnology4+0 100 50 2 50 50 BIO553

BIO504 Transport Phenomena 4+0 100 50 2 50 50 BIO554BIO505 Bio-Process Technology 4+0 100 50 -- -- -- --BIO506

BIO507

Bioinstrumentation

Training of 8 weeks after 4th

semester exams

4+0 100 50 -- -- --

50

--

Grand Total: 1350 24 600 300 08 200 250

Third Year - Sixth Semester


Paper Title Hours/week

MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.


BIO601 Recombinant DNA Technology

4+0 100 50 2 50 50 BIO651

BIO602 Operation Research 3+1 100 50 2 50 50 BIO652BIO603 Introduction to Bio-

Informatics4+0 100 50 2 50 50 BIO653

BIO604 Bioreactor Design and Operation

4+0 100 50 -- -- -- --

BIO605 Down Stream Processing 4+0 100 50 3 50 50 BIO655BIO606 Biomaterials 4+0 100 50 -- -- -- --Grand Total: 1300 24 600 300 9 200 200

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Fourth Year - Seventh Semester



MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.


BIO701 Environmental Biotechnology

4+0 100 50 2 50 50 BIO751

BIO702 Food Biotechnology 4+0 100 50 2 50 50 BIO752BIO703 Plant Tissue Culture 4+0 100 50BIO704 Bioanalytical Techniques 4+0 100 50 2 50 50 BIO754BIO705 Minor Project -- -- -- 5 75 75 --BIO706 Training of 4 weeks after

6th semester exams50

Grand Total: 1100 16 400 200 11 225 275

Fourth Year - Eighth Semester



MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.


BIO801 Major Project -- -- -- 5 150 150 --BIO802

BIO803

BIO804

Enzyme catalyzed organic synthesis

Project management and Entrepreneurship

Modeling and Simulation of Bio-Processes

4+0

4+0

4+0

100

100

100

50

50

50

3

3

50

50

50

50

BIO852

BIO854

BIO805 Elective – I * 4+0 100 50 -- -- -- --

Grand Total: 1100 16 400 200 11 250 250Options in Elective - I*1. Nanobiotechnology2. Microbial Biodiversity

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SYLLABUSBACHELOR OF ENGINEERING (BIOTECHNOLOGY)

THIRD SEMESTER

Paper Title: Concepts in Biotechnology-I (Theory)Paper Code: BIO 301 Max. Marks 100 Time: 3 Hours

Course Duration: 45 Lectures of one hour each. .Note for the Paper setter: The question paper should be divided into Section A and Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each section. SECTION-A

1. Introduction to Biotechnology: Historical perspectives, Emergence of modern biotechnology, Branches / scope of biotechnology

2. Biological systems in biotechnology: (a) Prokaryotic cell structure and systems: E.coli,

Bacillus

(b) Eukaryotic cell structure and systems: Saccharomyces, mammalian and non- mammalian cells in culture.

1. Basic techniques in biotechnology: Centrifugation : Principle, types, application, Electrophoresis : Principle, support media, protein and DNA electrophoresis, SDS-PAGE, Chromatography: Principle, types, application, Lyophilization : Principle, mechanism, application, Basic microscopy : Principle, various types of microscopes, introduction to electron microscopy, Radioisotopy : Basic Concepts, Various types of radio isotopes used in biology.

SECTION- B

2. Basic genetics : Mendelian inheritance, physical basis of inheritance, gene interactions., Bacterial and viral genetic systems., Genomic and mitochondrial DNA, C value paradox, cot curve, chromosome walking, Overview of DNA replication, genetic code, transcription, translation, Cytoplasmic inheritance, nucleo-cytoplasmic interactions.

3. Genetic manipulations: Concepts, enzymes involved in it, vectors, PCR, DNA sequencing,, construction and screening of a genomic library and c-DNA library containing foreign DNA sequences. Production of recombinant proteins.

6. Mutagenesis and protein engineering: Basic concepts only

BOOKS RECOMMENDEDa. Techniques used in Bioproduct Analysis-Biotol Series(I-IV), 1992, Buterworth

Heineman,UK.b. Basic Biotechnology By B..D. Singh (2004)

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c. Membrane Separation in Biotech by McGregor, C.W., 1986, Marcel Dekker Inc.,

New York.

b. Separation, Recovery and Purification in Biotech by Juan H., and Sengo, A.A., 1985, American Chem. Society, Washington.

c. Lab. Techniques in Biochemistry and Molecular Biology, 1984, Elsevier, New York. e. Physical Biochemistry,1982, Frieferder Freeman and Co., New York.

Paper Title: Concepts in Biotechnology-I (Practical)Paper Code: BIO 351 Max. Marks 50 Time: 2 Hours

Practicals:

1. Introduction to instrumentation: centrifuges, autoclaves, spectrophotometers, microscopes, laminar hoods, incubators, CO2 incubators, lyophilizer etc.

2. Polyacrylamide gel electrophoresis for proteins.3. Use of microscopes: compound microscope, fluorescent microscope,

inverted microscope and visit to E.M. lab.4. Use of Geiger muller counter.

Paper Title: Microbiology (Theory)Paper Code: BIO 302 Max. Marks 100 Time: 3 HoursCourse Duration: 45 Lectures of one hour each.

Note for the Paper setter: The question paper should be divided into Section A and Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each section.

SECTION-A 1. History of microbiology, Germ theory, Disciplines of microbiology,

2. Structure of microbes (bacteria, algae, fungi and viruses) 3. Microbial taxonomy including modern approaches of taxonomy such as DNA

taxonomy and numerical taxonomy, different groups in bacteria. 4. Microbial growth : synchronous & asynchronous, pure cultures, growth inhibitory

substances. SECTION-B 5. Microbial genetics, transformation, conjugation & transduction mutations.

6. Microbial Metabolisms :Nutrition, media and methods, effects of environment, metabolic products of industrial importance, metabolic pathways amphicatabolic and biosynthetic.

7 Microbial ecology (with particular reference to C, N cycles)8. Microbes as pathogens : Important microbial pathogens, toxins, mode of action of toxins

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Books Recommended:1. Microbiology by Pelczar M.J. and Chan. E.C.S.(Jr.), 2000, Tata

McGraw Hill Pub. Co., New Delhi.2 Medical Microbiology by Bernard, D.D., Dulbecco, R., Eisen, H.N.,

and Ginsbery, H.S., 1990, Harper and Row, New York.

3 Industrial Microbiology by Waites M.J. et al., 2001, Blackwell Science Ltd., London.

4 IInstant Notes in Microbiology by Nicklin J. et al., 2001, BIOS Scientific publishers, Ltd., U.K.

5. Microbiology: An Introduction by Tortora Benjamin/Cummings Pub. Co.,

Paper Title: Microbiology (Practical)Paper Code: BIO 352 Max. Marks - 75 Time: 3 HoursPracticals:

1. Experiments on isolation-spread plate, enrichment culture; staining and measurement of microbes; effect of environment on microbial growth; lyophilisation.

2. Testing of microbiological qualities of milk and water; growth and production formation-batch culture, enzyme production, assay of enzymes.

3. Microbial assay of antibiotics.4. Studies on auxotrophs5. Detection of an enzyme production by a microbe6. Determination of molecular weight of an enzyme.

Paper Title: Biochemistry (Theory)Paper Code: BIO 303 Max. Marks 100 Time: 3 HoursCourse Duration: 45 Lectures of one hour each.

Note for the Paper setter: The question paper should be divided into Section A and Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each section. SECTION-A1. Chemistry and properties of following Biomolecules:

a)amino acids b) proteins c) carbohydrates d) lipids e) nucleic acids, f) water soluble vitamins.

2. Chemical Bonds: Covalent bonds, co-ordinate bonds , hydrogen bonds,

Vander waal's forces , hydrophobic interactions , ionic bonds , dipole interactions.

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3. Proteins: Primary, secondary, tertiary and quaternary structures, methods for isolation and purification of proteins, protein analysis and amino acid sequence determination.

4. Carbohydrate metabolism: Glycolysis, glycogenolysis, glycogenesis and their regulation, citric acid cycle.

SECTION-B5. Fat metabolism: Oxidation of fatty acids, synthesis of fatty acids (fatty acid

synthase complex), ketone bodies.6. Amino acid metabolism: General pathways of amino acid metabolism ,

transamination, decarboxylation , deamination , Urea cycle. 7. Nucleic acid metabolism: Biosynthesis of purines and pyrimidine nucleotides,

biosynthesis of deoxyribonucleotides , their regulation , catabolism.8. Mitrochondria: Structure of mitochondria, organization of respiratory chain,

oxidative phosphorylation .10. Plant and microbial biochemistry- Photosynthesis and Nitrogen fixation

Books Recommended:a) Principles of Biochemistry, Lehninger, A.L., Nelson, DL and Cox, M. M. 4 th edition,

2005, Worth Publishers, New Yorkb) Harper's Biochemistry by Murray, R.K, 2006, 27th edition, Mc Graw – Hill, America.

c) Biochemistry by Zubay, G., 1993, W..C.Brown Publishers, Oxford, England.

d). Biochemistry by Stryer, L., 2005, 4th edition W.H. Freeman and Company, New York.

e) Biochemistry by Voet, D and Voet J.G., 2006, John Wiley and Sons, Inc. New York.

f) Outlines of biochemistry by Conn, E.C. and Stumph, P.K. 1995, John Wiley & Sons

Paper Title: Biochemistry (Practical)Paper Code: BIO353 Max. Marks 75 Time: 3 Hours

Practicals :Qualitative tests of carbohydrates (monosaccharides, disaccharides,

polysaccharides, reducing and non reducing sugars etc.), proteins and amino acids, paper chromatography of amino acids or sugars. Estimation of proteins ( by Biuret method and Lowry method) carbohydrates, DNA, RNA, Cholesterol, lipids etc by spectrophotometry.

Paper Title: Cell and Molecular Biology (Theory)

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Paper code: BIO 304 Max. Marks:100 Time:3HoursCourse Duration: 45 Lectures


SECTION-AMembrane structure and function, cytoskeleton, ECM and its role in cell behavior regulation. (5) Chromosomes and organization of DNA. (3)DNA Replication in Prokaryotes and Eukaryotes: Basic process, enzymes involved in replication. (6) DNA Recombination : A brief introduction to molecular mechanisms in Prokaryotes and Eukaryote(4)Insertion elements and Transposons: Background, types of IS elements and transposons. (4)

SECTION-BTranscription in Prokaryotes and Eukaryotes: Mechanism, factors in prokaryotes and eukaryotes, regulatory mechanisms like substitution of sigma factor, Lac operon in prokaryotes, regulation of gene expression in eukaryotes, post-transcriptional changes in eukaryotes, RNA editing. (8)Translation in Prokaryotic and Eukaryotic cells: stages, molecules involved, differential translation(6) Cell Cycle and its Regulation : Phases of cell cycle, cell cycle check points, brief introduction to factors involved in cell cycle regulation. (4)Signal Transduction : Signal transduction through receptor interacting with G-protein/ receptor with tyrosine kinase activity , role of second messengers like c-AMP, Inositol- tri- phosphate, Diacyl glycerol, Ca2+ ions, mechanisms. (4)

Books Recommended : Latchman,D., 2006 : Basic Molecular and Cell Biology Lewin, B., 2003 : Gene VIII, Oxford University Press, New York.Stansfield, W. D., Colome, J.S. : Schaum’s Outline of Theory and Problems of Molecular and Cano, R. J., 2004 and Cell Biology, Tata McGraw Hill Edition. Glick, B.R. and Pasternak, J. J., : Molecular Biotechnology, Principles and applications of 2004 recombinant DNA, Second edition, American Society for Microbiology Press, Washington D.C. Kendrew, S.J., 1994 : The Encyclopedia of Molecular Biology, Blackwell, U.K.Paper Title: Cell and Molecular Biology (Practical)Paper Code : BIO 354 Max. Marks : 50 Time : 2 Hours

1. Isolation of plasmid DNA from E.coli. 2. Isolation of Genomic DNA from E.coli cells.3. Agarose Gel Electrophoresis.4. Preparation of CaCl2 competent cells.5. Preparation of electrocompetant cells.

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6. Transformation of E.coli cells with the plasmid DNA by using CaCl2.7. Transformation of E.coli cells with the plasmid DNA by using electroporator.

Paper Title: Material and Energy Balance Calculations Paper Code: BIO 305 Max. Marks 100 Time: 3 HoursCourse Duration: 45 Lectures of one hour each.


SECTION-A1.Composition of mixtures and solutions, P-V-T relations for gas mixtures, Liquid and

liquid mixtures (vapour pressures, Raoult’s law, Henry’s law), Humidity calculations

2.Material balance for non-reacting systems.

3.Material balances for reacting systems.

SECTION -B

4.Material balances involving by pass, recycle and purge streams.

5. Energy balances: various forms of energy, type of systems, intensive/extensive

properties, general energy balance equation for a flow process, heat capacity, mean

heat capacity, energy balance for simple flow processes, use of steam tables.

6. Thermo chemical calculations.

Books Recommended:1. Felder, R. M., Roussean R. W., “Elementry Principles of chemical

Processes” (John Wiley & Sons)2. Himmelblau, D. M., “Basic Principles and calculations in

Chemical Engg.” (PHI)3. Bhatt, V. I., Vora, S.M., “Stoichiometry” (Tata McGraw

Hill)

11

Paper Title: Engineering Mathematics – IIIPaper Code: AS -306 Maximum Marks: 100 Time of examination: 3hrs.Course Duration: 45 lectures of one hour each.

Note for the Paper setter: The question paper should be divided into Section A and Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each section SECTION- ASequences and Series: Sequences, Limits of sequences, Infinite series, series of positive terms, Integral test, Comparison test, Ratio test, Root test. Alternating series, Absolute and Conditional Convergence, Leibnitz test. Power series: radius of convergence of power series, Taylor’s and Maclaurin’s Series, Formulae for remainder term in Taylor and Maclaurin series, Error estimates. (Scope as in Chapter 8, Sections 8.1 – 8.10 of Reference 2). (8 Lectures)

Complex Functions: Definition of a Complex Function, Concept of continuity and differentiability of a complex function, Cauchy – Riemann equations, necessary and sufficient conditions for differentiability (Statement only). Study of complex functions: Exponential function, Trigonometric functions, Hyperbolic functions, real and imaginary part of trigonometric and hyperbolic functions, Logarithmic functions of a complex variable, complex exponents (Scope as in Chapter 12, Sections 12.3 – 12.4, 12.6 – 12.8 of Reference 1). (8 Lectures)Laurent Series of function of complex variable, Singularities and Zeros, Residues at simple poles and Residue at a pole of any order, Residue Theorem (Statement only) and its simple applications (Scope as in Chapter 15, Sections 15.1 – 15.3 of Reference 1) (8 Lectures)

SECTION-B

Conformal Mappings, Linear Fractional Transformations (Scope as in Chapter 12, Sections 12.5, 12.9 of Reference 1). (8 Lectures)

Linear Algebra: Concept of linear independence and dependence, Rank of a matrix: Row – Echelon form, System of linear equations: Condition for consistency of system of linear equations, Solution by Gauss elimination method. Inverse of a matrix: Gauss – Jordan elimination method (Scope as in Chapter 6, Sections 6.3 – 6.5, 6.7 of Reference 1).(8 Lectures)Eigen values, eigen vectors, Cayley – Hamilton theorem (statement only). Similarity of matrices, Basis of eigenvectors, diagonalization (Scope as in Chapter 7, Sections 7.1, 7.5 of Reference 1). (7 Lectures)References:

1. E. Kreyszig. Advanced Engineering Mathematics, Eighth Edition, John Wiley.2. G. B. Thomas, R. L. Finney: Calculus, Ninth Edition, Pearson Education.

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3. Michael D. Greenberg. Advanced Engineering Mathematics, Second Edition, Pearson Education.

4. R. V. Churchill, J. W. Brown. Complex Variables and Applications, Sixth Edition, McGraw-Hill, Singapore, 1996.

5. Vivek Sahai, Vikas Bist. Linear Algebra, Narosa Publishing House, New Delhi, 2002.

WS357 Workshop Practice of 4 weeks after 2nd Semester exams:50 Marks

SYLLABUS

13

BACHELOR OF ENGINEERING (BIOTECHNOLOGY)FOURTH SEMESTER

Paper Title: Concepts in Bio-Technology-II (Theory)Paper Code: BIO 401 Max. Marks:100 Time: 3 hoursCourse Duration: 45 Lectures of one hour each.

Note for the Paper setter: The question paper should be divided into Section A and Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each section. SECTION-A

1. Applications of Bio-Technologya) Molecular diagnostics and therapeuticsb) Immunological diagnostic proceduresc) DNA diagnostics systemsd) Pharmaceutical diagnosticse) Vaccines

2. Genetic Engineering of plants and animals SECTION-B3. Bioremediation and Biosensors a) Clean up of oil spills b) Clean up of soil contaminated with pesticides etc. c) Heavy metal biosorption

d) Biodegradation of organic compoundse) Biosensors in the field of biology

4) Regulations and Patenting in Bio-Technologya) Biosafety levels of laboratoriesb) State level regulations to be followed in Bio-Technology laboratoriesc) Patents and biological products/processes

Books Recommended:1. Setlow, J.K., (Ed): Genetic Engineering: Principles and Methods, 20062. Glick BR, Pasternak JJ: Molecular Biotechnology, ASM Press, 2003

3. Buterworth Heineman :Techniques usedin Bioproducts Analysis-Biotol Series (I-IV),

1992 UK4. Ehebier :Lab. Techniques in Biochemistry and Molecular Biology, New York.5. Aastroel et al :Current Protocols in Molecular Biology, Green Publishing

Associates Inc and John Wiley & Sons, Inc

Paper Title: Concepts in Bio-Technology-1I (Practical)

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Paper Code: BIO 451 Max. Marks:50 Time: 2 hoursPracticals:

1. Isolation of DNA and its visualization through electrophoresis.2. Southern blot hybridization 3. Collection and cultures of microbes from some polluted spot and see their

degradable activity.4. Practice for filling up patent application.

Paper Title: Process ThermodynamicsPaper Code: BIO 402 Max. Marks: 100 Time: 3 Hours

Course Duration: 45 Lectures of one hour each.Note for the Paper setter: The question paper should be divided into Section A and Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each section.

SECTION-ALaws of thermodynamics, Application of laws of thermodynamics. Carnot Cycle, Properties of steam and sterilization. (15) Heat Effects:-Standard Heat of Formation, Heat of reaction; Free energy of formation; Refrigeration, Liquefaction of gases:- (Linde Hampson and Claude system). (6)

SECTION-B

Thermodynamics of solution; Raoult's law & Henry's law; activity coefficients; fluid phase equilibrium from equation of state; chemical reaction equilibrium; standard free energy change and equilibrium constant, effects of temperature on free energy change; equilibrium constant; equilibrium conversion. (16)

Applications of thermodynamics on bio-systems i.e. ce11, fermentation processes, substrate utilization (8). Books Recommended:

1. Vanes, Smith : Introduction to Chemical Engineering Thermodynamics, 6th Edition, McGraw Hill.

2. P. K. Nag : Engineering Thermodynamics 3. D. S. Kumar : Thermodynamics, S.K. Kataria and Sons.

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Paper Title: Chemical Reaction Engineering(Theory)Paper Code: BIO 403 Max. Marks: 100 Time: 3 HoursCourse Duration: 45 Lectures of one hour each


SECTION-AIntroduction and brief review of the Kinetics of Homogeneous reactions (04)Interpretation of rate data from Constant volume and Constant pressure systems. (08)Single ideal reactors (04)Design for single reactions (04)Biochemical Kinetics:Interpretation of batch kinetic data; kinetics of enzymes catalyzed reactions in free and immobilized states; Michaelis-Menton equation and its various modifications; (04)

SECTION – BDesign for multiple reactions (08)Biochemical Kinetics: Monod growth model and its various modifications; transport phenomena in bioprocess systems; Effects of external mass transfer in immobilized enzymes systems; analysis of intraparticle diffusion and reaction; kinetics of substrate utilization , product formation and biomass production. (06)Thermal Characteristics of Reactors: Temperature and pressure effects. (08)Books Recommended:Chemical Reaction Engineering Levenspiel,D(John Wiley)Chemical Engineering Smith, J.M.(Mc-Graw Hill)Reaction Kinetics for Chemical Enginners Wales, S.M.( Mc-Graw Hill)Chemical Reactor Theory - An introduction Denbigh,K.Turner,K.K.(Cambridge Press)Elements of Chemical Reaction Enginnering Scott Foggler, H.(PHI)

Paper Title: Chemical Reaction Engineering(Practical)Paper Code: BIO 453 Max. Marks: 50 Time: 2 Hours

1. Kinetic studies in a batch reactor.2. Kinetic studies in a plug flow reactor.3. Kinetic studies in a CSTR.4. Kinetic studies in a semi-batch reactor.5. RTD studies in CSTR.6. Dispersion number for packed bed reactor.7. Adiabatic batch reactor.

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Paper Title: Industrial Microbiology (Theory)Paper Code: BIO 404 Max. Marks : 100 Time: 3 HoursCourse Duration: 45 Lectures of one hour each.


SECTION-A1. Industrially important microbes (E. coli, Bacillus, Actinomyces, Saccharomyces ).2. Preparation of an ideal growth medium for production of biomass and a microbial

product.3. Strain improvement by genetic means. .4. Culture preservation.5. Classifications of microbia1 products.6. Microbial fermentations, Introduction to design of fermentors.

SECTION-B7. Microbial processes for: the production of organic acids, solvents, antibiotics enzymes, . polysaccharides, lipids, pigments & aroma8. Equipments and accessories for industrial processes.9. Microbial enzymes, their stability. Enzyme stabilization by selection and

genetical engineering; protein engineering, reaction environment rebuilding, chemical modification, intra-molecular cross linking and "immobilization, role of enzymes" in bio-conversions of industrially important compounds.

10. Genetically engineered microbes and their regulation: Introduction, their production, limitations and applications. .

Books Recommended:

1. M. J. Pelczar and E.C.S. (Jr) Chan, 2000 : Microbiology, Tata McGrawHil1 Pub. Co.; New"Delhi.

2. D. D. Bernard, R.Dulbecco, H.N.1990 Medical Microbiology, Harper and Row, New York.

3. M. J. Waites et al., 2001 : Industrial Microbiology, Blackwell Science Ltd., London.

4. Nicklin et al., : Instant Notes in Microbiology, BIOS Scientific Publishers, Ltd., U.K.

5. G.J.Tortora et al.1995:Microbiology: An Introduction. Benjamin/Cummings Pub. Co., Inc

Paper Title: Industrial Microbiology (Practical)

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Paper Code: BIO 454 Max. Marks: 50 Time: 2 Hours

Practicals :1. Microbial cell growth kinetics.2. Determination of size and density of the microbial cells; determination of thermal

death rate constant.3. Estimation of efficiency of a glass-wool air-filter; preparation of immobilized

whole cell system,.4. Production of ethanol , acids, solvents in microbial systems.

Paper Title: Immunology and Immunotechnology (Theory)Paper code: BIO 405 Max. Marks: 100 Time: 3HoursCourse Duration: 45 Lectures of one hour each.


SECTION-A1. Introduction and historical perspectives of immune system. (2)2. Cell and Tissues of immune system: Lymphoid cell, mononuclear cell,

granulocytes, mast cells, dendritic cells, primary lymphoid organs, lymphatic system, secondary lymphoid organs. (4)

3. Antigens and Haptens: Immunogenecity, chemical composition, susceptibility to antigen processing, immunogen dosage and route of administration, haptens, adjuvants. (4)

4. Antibody Structure, Function and Antibody Diversity: Basic structure, Immunoglobulin domains, classes. (6)

5. Major Histocompatiblity Complex: MHC molecules, cellular distribution, general importance. (3)

6. Antigen processing and Presentation to T cell: Antigen presenting cells and their role, pathway. (4)

SECTION-B 7. Antigen recognition and activation of immune response. (4)8. Autoimmunity : Organ specific and systematic autoimmune diseases. (3)9. Hypersensitive Reactions: Types, mechanisms of hypersensitivity. (3)10. Complement System: Components, Complement activation, consequences. (4)11. Antigen-antibody reactions, interaction, cross reactions, precipitation and

agglutination. 12. Immunoassays, radioimmunoassay, Enzyme linked immunosorbent assay,

Western blotting. (4)

Books Recommended:

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1. Goldsby, Kindt, T.J., and Osborne, B. A. 2000 : Kuby Immunology, W.H. Freeman & Co., NY. Fourth edition.

2. George Pinchuk, 2004 : Schaum’s Outline of Theory and Problems of Immunology, Tata McGraw Hill Edition. 3. Abbas, A. K., Lichtman, A. H. & Pober, J. S., 2000: Cellular and Mol.Immunology, . Philadelphia, fourth Edition., U.S.A

4. I.M. Riott, 2000 : Essential Immunology, ELBS/ Blackwell Scientific Publications, U.K.

Paper Title: Immunology and Immunotechnology (Practical)Paper Code: BIO 455 Max. Marks:50 Time: 2 hoursPracticals:

1. TLC and DLC for blood samples.2. Determination of cell number (viable/non-viable).3. Ficoll density gradient, separation of cell types.4. Immunization methods: Preparation of polyclonal antibodies.5. Determination of blood group antigens by hemeagglutination assay6. Radial immunodiffusion Assay.7. Ouchterlony Double Diffusion Assay.8. Characterization of immunobiologicals by ELISA.

Paper Title: Legal Laws & IPRPaper Code: BT 406 Max Marks: 100 Time: 3 HoursCourse Duration: 45 Lectures of one hour each.


SECTION- ABasics of Computer & Internet Technology:

Internet, ISP & domain name; Network Security; Encryption Techniques and Algorithms; DigitalSignatures.Introduction to Cyber World: Introduction to Cyberspace and CyberLaw; Different Components of Cyber Laws; Cyber Law and citizens.E-Commerce : Introduction to E-Commerce; Different E-Commerce Models; E-Commerce. Trends and Prospects; E-Commerce and Taxation; Legal Aspects of E-Commerce.

SECTION-BIntellectua1 Property Rights: IPR Regime in the Digital Society; Copyright and Patents; Intenational Treaties and

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Conventions; Business Software Patents; Domain Name Dispute and Resolution.

IT Act, 200l : Aims and Objectives; Overview of the Act; Jurisdiction; Role of Certifying Authority; Regulators under IT Act; Cyber Crime-offences and Contraventions; Grey Areas on IT Act.Project Work: Candidates will be required to work on a project. At the end of the course students will make a presentation and submit the project report.

Books Recommended:

1. Nandan Kamnath : A Guide to Cyber Laws & IT Act 2000 with Rules & Notification.

2. Keith Merill & Deepti Chopra : Cyber Cops, Cyber Criminals & Internet (IK Inter)

3. Diane Row Land : Information Technology Law.4. Vakul Sharma : Handbook of Cyber Laws (Mc.Mi1lian)

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SYLLABUS FOR BACHELOR OF ENGINEERING (BIO-TECHNOLOGY)

FIFTH SEMESTER

Paper Title: Enzyme Engineering & Technology (Theory)

Paper Code: BIO501 M. Marks: 100 Time: 3 hrs

Course Duration: 45 Lectures of one hour each.

Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each section.

Section - AIntroduction & Scope; General distinctive features and industrial applications; enzyme kinetics; single, substrate steady state kinetics; King-Altman’s method; inhibitors and activators; effect of pH and temperature; multi-substrate systems allosteric enzymes.Section - BImmobilization of enzymes; advantages; carriers; adsorption; covalent coupling; cross linking and entrapment methods; micro-environmental effect; enzyme reactors; reactors for batch/continuous enzymatic processing, choice of reactor type; idealized enzyme reactor systems; mass transfer in enzyme reactors; steady state analysis of mass transfer and biochemical reaction in enzyme reactors bio-process design; physical parameters, reactor operational stability; operational strategies; a few case studies.

Paper Title: Enzyme Engineering & Technology (Practical) Paper Code: BIO551 M. Marks: 50 Time: 2 hrs

Assay of enzymes; substrate specificity and efficiency of enzymatic catalysis; Kinetics of enzyme catalyzed reactions, Immobilization of enzymes, Microenvironmental effects in immobilized enzymes.

Books Recommended

Biotechnology By W.F. Guger & Gruger; 2003 (Text Book of Industrial Microbiology).Biochemical Engineering Fundmentals by J.E. Bailey and P.F. Ollis McGraw Hill Publications.Biotechnology Vol. 7 by H.J.R. Rehen and H.G. Reed; Beelar Publishers. Bioprocess Engineering Basic Concepts, by P.H. M.L.Shuler , F.Kargi :

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FIFTH SEMESTER B.E. (BIO-TECHNOLOGY) SYLLABUS

Paper Title : Bio-Process Engineering (Theory)

Paper Code : BIO 502 Max. Marks : 100 Time : 3 Hrs.

Course Duration : 45 lectures of one hour each.


Section - AKinetics of Microbial growth, substrate utilization and product formation in Batch, Fed-batch and continuous processes.

(10)Rheology of fermentation fluids and Scale-up concepts. (05)Introduction to modeling of growth kinetics: General structure for kinetic models, overview of structure and unstructured models.

(08)Section - B

Sterilization of media: design of heat sterilization processes; kinetics; Sterilization in place and Cleaning in place concepts.

(06)Sterilization of air : Filter sterilization and kinetics. (04)Design of fermentation media and optimization. (06)Aeration and agitation : various correlations and mass-transfer aspects, kLa determination.(06)

Paper Title : Bio-Process Engineering (Practical)


Study of different phases of microbial growth;Estimation of cell mass; Growth rate;mass and energy balance in a typical bioconversion process; Concept of limiting nutrient and effect of its concentration on cell growth;Study of growth inhibition kinetics;Comparison between aerobic and anaerobic bioconversion processes;Power consumption in a fermentation process and its correlation with rheology of the fermentation fluid ;effect of speed on the mixing time in a bioreactor;Estimation of kLa in a fermentation process.

Books Recommended:

1. Stainbury and whitakar : Principles of Fermentation Technology, Orgamon Press.

2. . M.L.Shuler , F.Kargi : Bioprocess Engineering Basic Concepts, P.H.

22

3. Nielson J. Villadsen J. : Bioreaction Engineering Principles.2nd Edition.

4. A.L. Demain; J.E. Davis : Manual of Indutrial Microbiology and Biotechnology, 1999 ASM Press, Washington DC.

5. M. Moo. Young : Comprehensive Biotechnology; The principles, applications and regulations of Biotechnology in Industry, agriculture and Medicine (4 Volumes) Pergamon, Publishres.


Paper Title: Animal Cell Culture & Bio-Technology (Theory)

Paper Code: BIO503 M. Marks : 100 Time: 3 hrs



Animal Cell metabolism, regulation and nutritional requirement; Animal cell growth characteristics and kinetics; nutrients, substrate and product transport through mammalian cell; primary & secondary culture; cell culture in continuous, perfusion and hollow-fiber reactor; mass transfer in mammalian cell culture; scale-up of cell culture processes; case studies; Gene transfer in animal cells and its applications; contamination & cyno presentation; Transgenese and transgenic animals including live stock; Transgenics as bioreactors ; Biotechnology or aquaculture, silkmoth, past control; Biodiversity, characterization, conservation; In vitro fertilization, embryotransfer technology; Stem cell Biology & Cloning.

Paper Title: Animal Cell Culture & Bio-Technology (Practical)

Paper Code: BIO553 M. Marks: 50 Time: 2 hrs

Establishment of Primary Culture; Lumphocyte culture ; Cell culture in static phase; and in spinner flasks; perfusion cell culture ; Metabolic study of culture cell;

Books Recommended:

1. Animal Biotechnology Vol ! and II by Griffith and Smith2. Animal Cell Culture by Freshney

23


Paper Title: Transport Phenomena (Theory)

Paper Code BIO 504 Max marks 100 Time 3 hours



SECTION-A

Transport of momentum, heat and mass by molecular motion-Newton’s law of viscosity, Fourier’s Law of heat conduction, Fick’s Law of Diffusion.

Transport properties-Viscosity, Thermal Conductivity and mass diffusivity.

Development of mathematical models of transfer processes through shell momentum balance for solving specific problems of transport of momentum in laminar flow or in solids in one dimension.

Development of general differential equations of fluid flow and their applications in solving one-dimensional steady state and unsteady state problems of momentum transfer.

Emphasis on the analogy between momentum heat and mass transfer with respect to transport mechanism and governing equations.

SECTION -B

Development of mathematical models of transfer processes through shell energy balance and shell mass balance for solving specific problems of transport of heat and mass in one dimension.

Development of general differential equations for heat transfer and mass transfer and their applications in solving one-dimensional steady state and unsteady state problems of heat and mass transfer.

Dimensional Analysis.

Books recommended:

Transport Phenomena Bird, R.B., Stewart, W.E.(John Wiley and Sons.)

Fundamentals of momentum Weity,J.R., Wilson, R.E.,and Wicks,C.E.(JohnWiley and Heat and mass transfer sons)

24

Momentum Heat and Mass Bennett, C.O., Myers, J.E.(McGraw Hill)Transfer

Paper Title: Transport Phenomena (Practical)

Paper Code: BIO 554 Max Marks 50 Time: 2 hrs

1. Thermal Conductivity of solids

2. Measurement of viscosity of liquids

3. Measurement of diffusivity of a binary system

4. Mass transfer coefficient for vaporization of naphthalene in air.

5. Heat transfer in natural convection.

6. Heat transfer in forced convection.

7. Wetted wall column: to find the mass transfer coefficient as a function of gas mass velocity in a wetted wall column.


Paper Title : Bioprocess Technology

Paper Code: BIO505 M. Marks 100 Time: 3 Hours



Section – AIntroduction : Characteristics and comparison of bioprocess technology with chemical technology. Substrates for bioconversion processes and design of media. Industrial application of cells and enzymes, Process technology for the production of cell Biomass, Ethanol, acetone-butanol, Citric acid, Dextran, aminoacids.

(15)Microbial production of some industrially important enzymes, Glucose isomerase, cellulose.(05)

Section – BProcess technology for the production of penicillin, tetracycline, steroid. Process technology for the production of vaccines, Bioenergy production; Bio-polymer design and synthesis; Fermentation technology for waste stabilization and bio-product synthesis.

(24)

25

Books Recommended:

1. S.O. Enfors and L.H. Hagstrom : Bioprocess Technology- fundamentals and applications,

Cambridge University Press.2. T.D. Brock : Biotechnology : A Text of Industrial

Microbiology, Smaeur associate (1990).3. L.E. Casida : Industrial Microbiology, wiley eastern Ltd. 1989.4. M.Y. Young (Eds) : Comprehensive Biotechnology, Vol. 1-4,

Pergamon Press.5. w. Crueger, A. Crueger : Bio-Technology, A Handbook of Industrial

Microbiology.6. Encyclopedia of Bioprocess Technology, Vol. 1-5.


Paper Title: Bioinstrumentation (Theory)

Paper Code: BIO506 M. Marks : 100 Time: 3 hrs


Note for the Paper-setter: Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each section..

Section - AIntroduction: Problems unique to bioinstrumentation; Lab View: A graphical programming language for virtual instrumentation; Basic Principles: Review of system concepts input/output characteristics, the black box signals linear, time-invariant systems static characteristics dynamic characteristics time versus frequency domain analysis Fourier Analysis; Fourier transforms of common and important signals windowing Discrete Fourier Transforms The Fast Fourier Transform Spectrum Analyzers windows Frequency Analysis transfer functions, frequency response magnitude and phase functions signals through systems 'ideal' and 'best' systems time vs frequency domain filters how to measure frequency response in the laboratory; Sample systems in the time and frequency domains; 0th, 1st, and 2nd order systems; Non-ideal systems noise and signal-to-noise ratio; nonlinearities and distortion products. wave-analyzer synchronous [lock-in] detection modulator, demodulator digital techniques analog to digital conversion signal averaging V. Transducers and associated electronics displacement transducers resistive strain gages bridge circuits capacitive displacement transducers piezoelectric transducers optical transducers temperature transducers;

26

Hemodynamic Measurements Blood pressure, pressure transcucers (manometers) extra-vascular techniques blood sounds stethoscopes intra-vascular techniques catheter-manometers Blood Volume and Flow indicator-dilution methods electromagnetic flowmeters ultrasonic methods .

Section - BBioelectric Potentials-Electrophysiology Origins of biopotentials dipoles remote versus local potentials muscle and nerve cells, intracellular potentials resting potential action potential stimulators discriminators histograms remote potentials evoked potentials; example: evoked potential audiometry Electrodes electrode interfaces fluid-fluid salt-fluid metal-fluid surface (skin) electrodes metal micro-electrodes glass micro-electrodes microelectrode preamplifiers Recording and Stimulating Systems, Putting it all together interference minimization and rejection stimulus isolation shielding grounding ground-loops; Sample recording system: electrocardiogram; ECG instrumentation Other cardiac devices ; Pacemakers power and pulse sources electrodes; Defibrillators Introduction to medical imaging

Books Recommended:1. James Diefenderfer :Principles of electronics Instrumentation,

Publishers: Words worth for Black Box, 1989.2. L.A. Geddes and Baker, L.E. : Principles of applied Biomedical Instrumentation,

A Wiley Interscience publication, 1989.3. Khandpur, R.S. : Handbook of Biomedical Instrumentation, Tata

McGraw Hill, Latest Edition.4. G. John : Medical Instrumentation application & Designs,

Webster, Publ., John Wiley & Sons, Inc.5. J.R. Cameron : Homodynamic Measurements, Medical Physics.6. J. Joseph Carr & M. John Brown : Introduction to Biomedical Equipment

Technology, Fourth Edition Pearson Education asia.7. Cromwell et al. : Additional Reading : Biomedical Instrumentation

and measurements, Pub: prentice Hall of India, New Delhi.

BIO507 Training of 4 weeks after 4th semester exams: 50 Marks

27

SYLLABUS FORBACHELOR OF ENGINEERING (BIO-TECHNOLOGY)

SIXTH SEMESTER

Paper Title: Recombinant DNA Technology (Theory)

Paper code: BIO601 Max. Marks:100 Time:3Hours

Course Duration: 45 Lectures.Note for the Paper setter : Total of 8 questions may be set covering the whole syllabus. Candidate will be required to attempt any 5 questions.

SECTION-A

1. Gene cloning and need to clone a gene; Isolation and purification of plasmid, chromosomal and genomic DNA from bacterial, plant and animal cells. (10) 2. Different cloning vectors like plasmids, cosmids, phagemids, shuttle vectors, and other vectors for plant and animals; enzymes used in recombinant DNA technology like restriction endonucleases, ligases, polymerases, kinases and phosphatases. (10)

SECTION-B

3. Cloning of a specific gene; studying gene location and structure; studying gene expression; expression of foreign genes in research and biotechnology; maximization of recombinant proteins; brief introduction to sequencing and site directed mutagenesis, different types of PCR and applications; safety measures and regulations for recombinant DNA work (15) 4. A brief introduction to the followings: phage display system, Yeast two hybrid system, and RNAi technology. (5)

5. Applications of recombinant DNA technology in the fields of Medicine, Agriculture, Forensic and Environment. (5)

Paper Title: Recombinant DNA Technology (Practical)

Paper code: BIO651 Max. Marks:50 Time:2Hours

Digestion of plasmid DNA by restriction endonuclease; Ligation assay; Amplification of DNA using PCR; RAPD PCR; Induction and expression of a gene cloned in an expression vector in E.coli..

28

Books Recommended :

1. Watson, J.D., et al. : Recombinant DNA: Genes and (3rd ed.) Genomes,McGraw Hill Publications, 2007 2. Brown, T.A. (4th ed.) : Gene Cloning and DNA analysis, An Introduction,Blackwell Science. 3. Watson, J. (5th ed.) : Molecular Biology of the Gene, Benjamin, Cummings, Pearson education, Schweiz AG, Germany, 2004. 4. Primrose, S.B. and Twyman, R.M.: Principles of gene manipulation, Blackwell and Old, R.W. (6th ed.) Science, U.K. 5. Alberts, B. et al . : Molecular Biology of Cell, Garland Publishers Inc.,London,1994.

SIXTH SEMESTER B.E. (BIO-TECHNOLOGY) SYLLABUSPaper Title: Operations Research

Paper Code: BIO602 Maximum Marks: 100 Time of examination: 3hrs.

Course Duration: 45 lectures of one hour each.

Note for the paper setter: Total of 8 questions may be set covering the whole syllabus. Candidate will be required to attempt any 5 questions selecting at least two from each part.

Syllabus:

PART AOptimization Problems. Linear Programming: Graphical Method (Scope as in Chapter 1 of Reference 1).Solution of simultaneous linear equations: An overview (Scope as in Chapter 2, Sections 2.15 – 2.16 of Reference 1).Basic solutions, lines and hyperplanes, convex sets, extreme points, convex sets and hyperplanes (Scope as in Chapter 2, Sections 2.19 – 2.21 of Reference 1).Reduction of any feasible solution to a system of equations to a basic feasible solution. Simplex Method: The simplex algorithm (Scope as in Chapter 3, 4 of Reference 1).

Tableau format for simplex computations, Charne’s M-method, Two phase method (Scope as in Chapter 5 of Reference 1).The revised simplex method (Scope as in Chapter 7 of Reference 1). (12 Lectures)Duality theory: Formulation of the dual problem, Theorems on duality: Weak Duality Theorem, Strong Duality Theorem, Complementary Slackness Theorem, Dual Simplex Algorithm (Scope as in Chapter 8, Sections 8.1 – 8.12 of Reference 1). (6 Lectures)Integer Linear Programming: Branch and Bound Algorithm, Cutting Plane Algorithm (Scope as in Chapter 9, Section 9.1 – 9.2 of Reference 2). (4 Lectures)

PART BTransportation Problem: Initial solution by North-West corner rule, Row minima method, Column minima method, Matrix minima method, Vogel’s method. Tableau of transportation problem, u-v algorithm

29

for solving transportation problem. Degeneracy in transportation problem. (Scope as in Chapter 9 of Reference 1). (6 Lectures)The Assignment Problem: Hungarian Method (Scope as in Chapter 5, Section 5.4 of Reference 2).(2 Lectures)

Traveling Salesman Problem (Scope as in Chapter 9, Section 9.3 of Reference 2). (2 Lectures)Dynamic Programming: Shortest route problem, Knapsack Model, Workforce size model, Equipment replacement model, Investment model, Game of chance (Scope as in Chapter 10, Sections 10.1 – 10.3, Chapter 15, Section 15.1 – 15.2 of Reference 2). (6 Lectures)CPM and PERT: Network representation, Critical path computations, Construction of time schedule, Linear programming formulation of CPM, PERT networks (Scope as in Chapter 6, Section 6.6 of Reference 2). (2 Lectures)Basic Queuing Systems: Elements of a queuing model, Pure birth and pure death model, Generalized Poisson queuing model (Scope as in Chapter 17, Section 17.1 to 17.5 of Reference 2). (5 Lectures)References:

1. G. Hadley. Linear Programming, Narosa Publishing House, New Delhi, 2002.2. Hamdy A. Taha. Operations Research, An Introduction, Seventh Edition, Pearson

Education, Delhi, 2003.3. Kanti Swaroop, P. K. Gupta, Man Mohan. Operations Research, Twelfth Edition,

Sultan Chand and Sons, New Delhi, 2004.4. A. M. Natarajan, P. Balasubramani, A. Tamilarasi. Operations Research, Pearson

Education, Delhi, 2005.

Paper Title: Operation Research (Practical)

Paper Code: BIO652 Max. Marks 50 Time: 2 Hours

Practicals based on theory paper code BIO602

SIXTH SEMESTER B.E. (BIO-TECHNOLOGY) SYLLABUS

Paper Title: Introduction to Bioinformatics (Theory)

Paper Code: BIO 603 M. Marks : 100 Time: 3 hrs



Section –A

Introduction to Bioinformatics: History of Bioinformatics; Objectives and areas of Bioinformatics; Genome sequencing projects; Human Genome Project - history, techniques and insights.Introduction to databases:

30

Basic concept of database, Type of databases; Literature Databases-PUBMED, MEDLINE;Nucleic acid and protein databases- GenBank, EMBL, DDBJ, SWISS PROT, UNIPROT;Human, animal and plant databases- Ensembl, Genome project TIGR database, Maize

GDB etc.Structural databases- PDB, PDBsum, NDB etc;Motifs and Pattern Databases-

PROSITE, Pfam, BLOCKS, PRINTS etc;Database Retrieval and deposition systems-

SRS, Entrez, Bankit, Seqin, Webin, AutoDep.

Basic Sequence Analysis: Protein Sequence Analysis- composition, hydropathy, flexibility, pattern, motif etc;Nucleic acid Sequence Analysis- Composition, motif, restriction site, primer design etc.Sequence Homology: Scoring matrices, Local and global alignment concepts, Dot matrix sequence comparison, Dynamic programming; Statistics of alignment score;Database searches for homologous sequences- FASTA, BLAST, PSI-BLAST and PHI-BLAST;Multiple sequence alignment: CLUSTALW, PILEUP;Evolutionary analysis- Concept of phylogeny and trees, Relationship of phylogenetic analysis to sequence alignment.Gene prediction: Gene prediction in prokaryotic and eukaryotes genomes; evaluation of gene prediction methods.

Section - BProtein structure prediction: Prediction of protein secondary structure from the amino acid sequence- Chou-Fasman/GOR method, JPRED, PSIPRED, PHD;Prediction of three-dimensional protein structure-Homology-based structure prediction, Fold recognition and ab initio methods for structure prediction;Evaluating the success of structure predictions-CASP and CAFASP.Introduction to the concepts of molecular modeling: Molecular structure and internal energy; Molecular Mechanics; Energy Minimization and related methods for exploring the energy surface; Molecular Dynamics, Conformational analysis;Use of molecular graphics packages- Rasmol, MOLMOL, Chimera, Pymol, spdbviewer. Computer Aided drug design: Drug discovery process; Role of molecular recognition in drug design; Concepts in Quantitative structure activity relationships (QSAR); Docking problem, Concepts of docking; Structure based Drug design.Applications of Bioinformatics:

31

Comparative Genomics; Proteomics; Gene expression informatics; Metabolomics; Computer aided vaccine design.

Text / Reference Books:

Mount D. W. (2004). Bioinformatics & Genome Analysis. Cold Spring Harbor Laboratory Press. Baxevanis B.F. and Quellette F. (2004). Bioinformatics a Practical Guide to the Analysis of Genes and

Proteins. Wiley-Interscience. Eidhammer I., Jonassen I. and Taylor W. R. (2004). Protein Bioinformatics: An algorithmic approach to

sequence and structure analysis. Mathematics. Orengo C.A., Jones D.T. and Thornton J.M. (2003). Bioinformatics: Genes Proteins and Computers. Bios

Scientific Pub. Bourhe P. E. and Weissig H. (2003). Structural Bioinformatics (Methods of structural Analysis). Wiley-Liss. C. Braden and C. Tooze (1991). Introduction to Protein Structure” Garland Publishing Inc., New York. Jiawei Han, Micheline Kamber, Data Mining Concepts and Techniques- Morgan Kaufmann publisher, 2001. S.C.Rastogi, N.Mendiratla and P.Rastogi (2004)., Bioinformatics methods and applications- Genomics,

Proteomics and Drug Discovery, Prentice Hall (India)., T.B.Kitano (2003), Handbook of Comparative Genomics: Principles and Methodology, Graziano Pesole. Dov Stekal (2003) Microarray Bioinformatics, Cambridge University Press, Cambridge. Brown TA. (2002) Genomes. John Wiley & Sons (Asia) Pvt. Ltd. Singapore. Charles R. Cantor, Cassandra L. Smith (1999) Genomics: The Science and Technology behind the Human

Genome Project, John Wiley & Sons (Asia) Pvt. Ltd. Singapore. P.Clate & R.Backofen (1998), Computational Molecular Biology, Willy Publication, T.K.Atwood and D.J. Parry Smith, Introduction to Bioinformatics. Biological databases: chapters 3 and 4


Paper Title: Introduction to Bioinformatics (Practical)

Paper Code: BIO 653 Max Marks 50 Time: 2 hrs

To Study & analyse various biological databases at NCBI, EBI, Expasy, NBRF-PIRNucleic acid sequence databases like Gene Bank, EMBL etc.Protein sequence databases SWISSPROT, UNIPROT etc.Structural databases- PDB, NBD

To retrieve sequences from NCBI/EBI/ExPasy using ENTRZ, SRS

Similarity searches using various tools likeBLAST/ FASTA, BLAST N, BLAST P, BLAST X

CLUSTALW / Phylogenetic analysis tools To predict gene/ORF for genomic DNA sequences of prokaryotic and eukaryotic

origin. To analyze protein sequence using Secondary Structure prediction Methods:

Chou-Fasman/GOR method, JPRED, PSIPRED, PHD etc. Energy minimization using SPDBV. To down-load structures of proteins in software like RASMOL, SPDBV and

analysisof structures in these software

Fold recognition

32

Homology modeling using SPDBV.


Paper Title : Bio-Reactor Design and Operation (Theory)


Course Duration : 45 lectures of one hour each.


Section - A Introduction to reactor design and Kinetics of Bioreactions (review). (1)

Biological systems : Organism selection; bacterial, yeast and fungal cultures; Effect of microorganism type and culture characteristics on bioreactor design and operation. (6)

Ideal Reactors : Batch reactor(Closed and fed-batch), continuous reactors; PFTR, CSTR design equations. Reactors for biomass growth; reactors in series; recycle reactors; overview of Pneumatically agitated bioreactors; Membrane bioreactors and Photo bioreactors. (10)RTD in reactors: Models for non-ideal reactors; Tanks in series and dispersion models.

(6)

Section - BInstrumentation and control of various parameters in bioreactors for Dissolved oxygen, foam,pH, temperature, flow, pressure, microbial biomass,CO2 etc; Methods of measuring process variables and control systems: Proportional, Proportional integral and Proportional integral derivative. (8)

Operation and scale-up of bioreactors. (4)

Bioreactor modeling and stability: Fermentation dynamics, Biomass production and dilution factor, Thermal stability concepts. (6)

Mechanical design Concepts: Application to continuous sterilizers, RTD concepts, Application of design principles. (4)

33

Books Recommended:

1. M.L.Shuler , F.Kargi : Bioprocess Engineering Basic Conepts, P.H

2. J.A.Asenjo and J.C.Merchule : Bioreactor Sytem Design, dekker. (Eds.)

3. KK. Van’t R et and J. Tramper : Basic Bioreactor Design, marcel Decker,1992.

4. J.M.Lee : Biochemical Engineering, PH 1992.

5. B. Atkinson : Biochemical Engineering and Biotechnology

Handbook, Stockton Press, N.Y. 19926. Biotechnology Volume 2 and 4, VCH, 1991.


Paper Title : Downstream Processing




Section – A

Introduction; An overview of Bioseparation, Separation of cells and other insolubles from fermented broth; Filtration and microfilteration; Centrifugation (batch, continuous). Designing of centrifuges for desired product of desired capacity; Cell disruption: Physical methods – osmotic shock, grinding with abrasives solid shear, liquid shear, Chemical methods- alkali reagents, enzymatic methods; Product isolation: Extraction and adsorption method, solid-liquid separation, liquid-liquid separation, distillation, precipitation method using ammonium sulfate, organic solvents, high molecular weight polymers, reverse osmosis;

34

Section – B

Electrophoresis and chromatography principles for product purification. Different electophoresis techniques viz. isoelectric focusing, chromatographic techniques viz. paper, gel filtration, column, ion exchange, affinity, GLC, HPLC. Dialysis, ulterafilteration; Product polishing: crystallization and drying.

Paper Title: Down Stream Processing (Practical)

Paper Code: BIO655 Max. Marks 50 Time: 2 Hours

Cell lysis and release of cell contents; Use of centrifugation, ultra centrifugation Ultra filtration, lypophilization, crystallization, HPLC for biosparation.

Books Recommended:

Bailly & Ollis Biochemical Engg. – Academic Press.H.Gunzler – Handbook of Analytical techniques – Wiley PublicationsH.J.Rehm and G.Reed, Biotechnology – Vol. 3, 4, 5 verlar PublishersHumphrey, Aiba and Miller, Biochemical Engg. Academic PressMurray Moo-Young Comprehensive biotechnology, Vol. II latest ed. Pergan Publishers.Product Recovery in Bioproce. Technology, Heinemann, Butterworth Publication.Stanbury & Whitteker, Principles of Fermentation Technology, Pergamon PressWestermeier – Electrophoresis in Practice, Wiley Publications.Willard e. al., International methods of Analysis – CBS PublicationWilson and Golding, A Biologist’s Guide to Principles & Techniques of Practical Biochemistry, Cambridge University Press.Principles of Fermentation Technology By P.F. Stainbury , A. Whitaker and S.J. Hall, 1997. Aditya Books (p) Ltd. New Delhi.Biotechnology by Rehm and Reed Vol. 7(a).Comprehensive Biotechnology By Murray Moo Young.

Paper Title : Biomaterials



35


Section - AIntroduction to biomaterials, Characterization of materials; mechanical properties; thermal properties; surface properties and adhesion; Various classes and forms of biomaterials;

Metals : Stainless steel, Cobalt- Chromium Alloys, Titanium based alloys other metals, metallic corrosion and luological tolerance.

Cermics : Carbons alumina, recoverable ceramics, composites, ceramic surface analysis

Synthetic polymers : Polymers in luomedial use, polyethylene, polypropylene, perfluorinated polymers. Acrylic polymers, hydrogels, polyurethanes, polyamide, silicone rubbers, polymer sterilization.

Biopolymers : Collagen, Elastin, Mucopolysarrharides, Proteoglycans Cellulose and derivatives and other.

Tissue grafts : Blood, Tissue grafts and rejection processes, shim and grafts

Soft tissue speciation : Space filters maxillofacial and fluid transfer implants, biomaterials in urological practice.

Section - B

Cardiovascular implants and extra-coronial devices : Blood clotting, Blood Theology, Blood Vessels, The Heart, Lungs and Vascular implants, Blood substitutes.

Biomaterials in ophthalmology : Anatomy of eye, Viscoelastic Solution Contact Lens and Optical implants, Skeletal looking material for retinal detachment Vitreous implants artificial tears.

Orthopedic implants : Bone composition and materials, fixation devices, Fracline healing by clinical and dedromagnetic stimulation hip joint replacement, Knee joint repair, bone regeneration with restorable materials.

Dental Materials : Tooth composition and mechanical properties impression materials, filling and restorative materials, metal in dentist, oral implants use of collagen in dentist

Books Recommended :

1. Sujata V. Bhat : Biomaterials (2nd Edition)

36

2.Finman : Biomaterials

3. Ratner : Biomaterial and science

B.E. (Biotechnology) Fourth Year-Seventh Semester (Syllabus)

BIO701 Environmental Biotechnology

Total Number of lectures: 45 Maximum marks: 100


SECTION-A

Introduction: Environmental Pollution, Sources and effects. Biodegradation and

Bioremediation- definitions and examples.

(5)

Waste Water Treatment: Aerobic and Anaerobic waste water treatment. Kinetics for

waste water treatment processes. Different type and design of Aerobic and Anaerobic

reactors, Methanogenesis. Emerging biotechnological processes in waste water treatment.

(18)

SECTION-B

Solid Waste management: Biodegradation of pollutants, Treatment processes for solid

waste, Use and Management, Bioremediation of contaminant lands. Hazardous wastes:

Source management and safety.

(10)

Microbial Leaching and Mining: Recovery of metals from solutions, microbes in

petroleum extraction, microbial desulfurization of coal.

(7)

Environmental Genetics: Degradative plasmids, release of genetically engineered

microbes in environment.

(5)

37

Practicals:

To determine BOD & COD levels of the sample. Application of microbial approaches in the remediation of contaminated soils. Analysis of contaminated and bioremediated samples.

Reference Books1. Bruce E. Rittmann & Perry L. McCarty. Environmental Biotechnology: Principles

& Applications (1st Edition), McGraw-Hill, 2000.2. Michael T. Madigan, John Martinko & Jack Parker. Brock Biology of

Microorganisms (10th Edition). Prentice Hall, 2002.3. George Tchobanoglous, Franklin L. Burton Editor & H. David Stensel

Wastewater Engineering: Treatment and Reuse (4th Edition). McGraw-Hill, 2002.

4. Comprehensive Biotechnology (Vol. 1-4): M. Y. Young (Eds.), Pergamon Press, Oxford.

Paper Code : BIO 702 Food Biotechnology Max.Marks:100

Course duration: 45 Lectures of one hour each.

Note : The question paper should be divided into Section A and Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each sectionSECTION-A

History of Microorganisms in foods (2)Primary sources of Microorganisms in foods (3)Synopsis of common food borne bacteria, fungi and yeasts (6) Incidence & Behaviour of Microorganisms in foods (3)Extrinsic & Intrinsic parameters of foods (4)Role and Significance of Microorganisms as Single cell proteins, Food value of Mushrooms, Yeasts, Production of Fermented foods

(3)

SECTION-BFood types and their physical & chemical properties (4)Food Spoilage (3)Food Borne diseases (6)Food Preservation (5)Diagnosis of microbial contents of food: Classical & Molecular approach (4)Food Biosensors (2)

38

http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=George%20Tchobanoglous/104-8398022-5365527

http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=H.%20David%20Stensel/104-8398022-5365527

http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=Franklin%20L.%20Burton/104-8398022-5365527

Practicals:

Microbiological Examination of food/s. Enumeration and detection of food borne organisms. Estimation of quality of milk-by dye reduction, direct microscopic count, Determination of diacetyl, titrable acidity in the milk sample.

Reference books1. Morten Meilgaard, Gail Vance Civille & B. Thomas Carr. Sensory Evaluation

Techniques (3rd Edition). CRC Press, 1999.2. Daniel Charles. Lords of the Harvest: Biotech, Big Money, and the Future of

Food (1st Edition). Perseus Books Group, 2001.3. Adams, M.R. and M.O. Moss. Food Microbiology. Turpin Distribution Service

Ltd., Blackhorse Road, Letchworth, Herts SG6 1HN, UK, 1995.4. Gauri Mittal.Food Biotechnology: Techniques and Applications. CRC Press,

1992.5. Banwart, George J. Basic Food Microbiology, 2nd ed. AVI/ Van Nostrand

Reinhold Publishing Co, 1989.6. Cliver, D.D. Foodborne Diseases. Academic Press, Inc, 1990.7. Food and Drug Administration. Bacteriological Analytical Manual (BAM) (8th

Edition). AOAC, Arlington, VA, 1995.

BIO703 Plant Tissue Culture



Section A

Introduction, requirements and Techniques. (4)Tissue Culture Media and Cell Culturing. (5)Cellular Totipotency, Somatic Embryogenesis, Synthetic seeds. (6)

39

http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=Gauri%20Mittal/104-8398022-5365527

http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=Daniel%20Charles/104-8398022-5365527

http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=B.%20Thomas%20Carr/104-8398022-5365527

http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=Gail%20Vance%20Civille/104-8398022-5365527

http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=Morten%20Meilgaard/104-8398022-5365527

Haploid Production: Zygotic Embryo Culture: Morphogenesis in the culture of seeds with partially differentiated embryos.

(5)

Section B

Microsurgical experiments, Morphogenic potential of the embryo callus, practical applications.

(5)In-Vitro Pollination and Fertilization: Introduction, Terminology, In-Vitro Pollination, Applications.

(5)Genetic engineering and production of pathogen free plants: Gene expression, genetic stability, and field performance

(5)Introduction, Strategies used to optimize product yield, commercial aspects, Germplasm storage: Introduction, long term Storage, Short or Medium term storage.

(10)

Reference Books:

1. Adrian Slater, Nigel W. Scott & Mark R. Fowler. Plant Biotechnology: The Genetic Manipulation of Plants (1st Edition). Oxford University Press, 2003.

2. Roberta H. Smith. Plant Tissue Culture: Techniques and Experiments (2 Edition). Academic Press, 2000.

3. Lydiane Kyte, John Kleyn & John G. Kleyn. Plants from Test Tubes: An Introduction to Micropropagation (3rd Edition). Timber Press, 1996.

4. J. Heslop-Harrison, John H. Dodds & Lorin W. Roberts. Experiments in Plant Tissue Culture (3rd Edition), Cambridge University Press, 1995.

BIO 704 Bioanalytical Techniques Max. marks: 100 Time:3 Hours

Course Duration : 45 lectures of one hour each.Note for the paper setter: The question paper should be divided into section A and section B. Total of 8 questions, 4 from section A and 4 from section B are to be set. The students will be required to attempt 5 questions selecting at least 2 from each section.

Section-ASpectrophotometry(UV and Visible): Principle, single beam and double beam spectrophotometer, factors influencing the absorption spectra, overview of empirical rules, solvent perturbation method and difference spectroscopy; various applications of absorption spectroscopy w.r.t. biotechnology.(05)

40

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Spectrofluorimetry: Principle, significance and various details related to instrumentation. (03)Atomic absorption Spectrophotometry: Principle, instrumentation details, various interferences in atomic absorption spectroscopy and applications.(04)Infrared and Raman Spectroscopy: Principle, factors deciding the spectra, instrumentation, overview of different class of compounds and their IR spectra. Introduction to Raman scattering.(04)Nuclear Magnetic resonance: phenomena of resonance, instrumentation, diamagnetic shielding, anisotropy, chemical shift, free induction decay (FID), population distribution of nuclei, and prediction of NMR spectra on the basis of (n+ 1) rule for basic class of compounds. Overview of electron spin resonance spectroscopy (ESR) and magnetic resonance imaging (MRI).(08)

Section-BElectron Microscopy: Transmission and scanning electron microscopy, significance of vacuum, basic instrumentation for TEM and SEM, sample preparation for electron microscopy. Overview of Atomic force microscopy and tunneling microscopy w.r.t. their working principle and comparison with other scanning techniques. (05)Crystallography and X-ray diffraction: Introduction to x-ray and general theory and instrumentation, Bragg’s law, various techniques to determine crystal structure.(05)Radioisotope techniques: radiotracers, units of radioactivity measurement, proportional and scintillation counters, introduction to autoradiography and nuclear medicine.(06)Mass Spectroscopic techniques: Introduction to mass-spectroscopy, significance, instrumentation details of a mass-spectrometer, ionization techniques, single and double focusing, alternate mass separation techniques- time of flight and quadruple. Interface of mass-spectra with liquid and gas chromatography (LC-MS and GC-MS)(05)Practicals: Different experiments to confirm spectroscopic laws, practical demonstration of the above mentioned techniques.Suggested Books:1. Keith Wilson & John Walker : Principles and Techniques of Practical

Biochemistry (5th Edition), Cambridge University Press,2000.

2. William Kemp : Organic spectroscopy. English Language Book Society & The Macmillan Press Ltd.3. David Friefelder : Physical Biochemistry, Freeman & Co.

New York4. P. Carmona, R. Navarro,A. : Spectroscopy of Biological Molecules:

Modern trends( Ist Editon), Kluwer Academic Publishers, 1997.

BIO 705 Minor Project

BIO706 TRAINING OF 4 WEEKS AFTER 6th SEMESTER EXAMS.

41

SYLLABUS FOR BACHELOR OF ENGINEERING (BIO-TECHNOLOGY) EIGTHTH SEMESTER

BIO 801 Major Project

BIO 802 Enzyme Catalysed Organic Synthesis



SECTION-A

Biocatalysis: Definition of Biocatalysis, advantages and disadvantages of Biocatalysis

over chemical catalysis. Different types of Biocatalysis; microbial ,enzymatic and

enzymatic and immobilized system of Biocatalysis; current industrial biocatalysis with

different enzymes.

Immobilized enzymes for Biocatalysis.

(15)

Industrial processes: Comparing different approaches.

(8)

SECTION-B

Stereoselective biocatalysis for the synthesis of chiral pharmaceutical intermediate such

as synthesis of ACE inhibitors ,definition,mode of action of inhibitors ,recent

developments synthesis of anticholesterol drug by biocatalysis routs , calcium channel

blocking drugs, potassium channel openers,antiviral.

(22)

Practicals: Optimization of enzymatic catalysis reaction conditions.

Enzymatic catalysis reaction with activators and inhibitors.

Product identification using different analytical technique.

42

Reference Books1. Andreas S. Bommarius, Bettina R. Riebel. Biocatalysis: Fundamentals & Applications, Wiley VCH, 2004.2. Lawrence P. Wackett and C. Douglas Hershberger Biocatalysis and Biodegradation: Microbial Transformation of Organic Compounds. ASM Press, Washington DC, 2001.3. Stanley M. Roberts, Nicholas J. Turner, Andrew J. Willets, Michael K. Turner.

Introduction to Biocatalysis: Using Enzymes and Microrgansims, Cambridge University Press, 1995.4. Sheldon, R.A., Porskamp, P., and Ten Hoeve, W. Advantages and Limitations of

Chemical Optical Resolution, in Biocatalysis in Organic Synthesis, Tramper, J., Van der Plas, H.C., and Linko, P. (Eds.) Elsevier, Amsterdam, 1985.

BIO 803 Project Management and Enterpreneurship


Note for the paper setter: The question paper should be divided into section A and section B. Total of 8 questions, 4 from section A and 4 from section B are to be set. The students will be required to attempt 5 questions selecting at least 2 from each section.

Objective: The course aims at acquitting the students with the subject of project management and also nature, significance and problems of entrepreneur with special reference to India. Section-A

Project Formulations and Planning ,Private commercial criteria for project choice, project cycle, feasibility, marketing feasibility, Financing for Projects and financial feasibility, Project Implementation. Brief outline of social cost benefit analysis: rationale, UNIDO and little Mirrlees approaches, UNIDO-IDCAS manual, shadow prices and conversion factors, applications in India. Planning and scheduling networks, critical path, PERT model, CPM model, PERT/cost, resource leveling and allocation.

Section-B

Entrepreneur- Concept on percent - Functions and clarifications of entrepreneurs - Characteristics of entrepreneur - Nature and importance of ,entrepreneur - Entrepreneur vs. professional manager - Women entrepreneurs. Concept of Entrepreneurship - Entrepreneurship and environment-Policies governing entrepreneurs, entrepreneurial development programmes - Institutions for - entrepreneurship development, entrepreneurship. Entrepreneurship -Entrepreneurship development in other countries. Institutions for Entrepreneurial Development - Role of constancy organizations - Role of financial institutions -Bank finance to entrepreneurs Entrepreneurship development: Role of development financial institutions.

43

Books Recommended1. UNIDO: Guidelines for Project Evaluation, United Nations, 1972.2. Mannual for the preparation of Industrial Feasibility Studies, United Nations

1978.3. Manual for Evaluation of Industrial Projects, United Nations, 1980.4. IMD little and J.A. Mirrlees: Project Apraisal and Planning in Developing

Countries.5. Prasanna Chandra: Projects: Preparation, Appraisal Budgeting and Control.6. Vasanta Desai: Dynamics of entrepreneurial development and management.7. Vasanta Desai: Entrepreneurial development.8. Peter F. Drucker: Innovation and development.

BIO 804 Modeling and Simulation of Bioprocesses



SECTION-A

Types of kinetic models.Data smoothing and analysis. (8)

Mathematical representation of bioprocess; parameter estimation; numerical integration techniques; parameter sensitivity analysis; statistical validity.

(10)

Discrimination between two models. Physiological state markers and its use in the formulation of a structured model.

(5)SECTION-B

Development of compartment and metabolic pathway models for intracellular state estimation.

(10)Dynamic simulation of batch, fed-batch steady and transient culture metabolism; Numerical optimization of Bioprocesses using Mathematical models.

(12)

Practicals:To design mathematical models from the provided data.

44

Reference Books1. John Ingham, Irving J. Dunn, Elmar Heinzle & J.E. Prenosil. Chemical

Engineering Dynamics: Modelling with PC Simulation (2nd Edition). Wiley-VCH, 2000.

2. Jonathan B. Snape, Irving J. Dunn, John Ingham & J.E. Prenosil. Dynamics of Environmental bioprocesses: Modelling and Simulation. Wiley-VCH, 1995.

3. Dieter Behrens & P. Kramer (Eds.). Bioreactors, Downstream Processing, Process and Reactor Modelling and Bioprocesses. Wiley-VCH, 1988.

4. Dunn. Biological Reaction Engineering. John Wiley & Sons Inc

ELECTIVE - 1

BIO805 Nanobiotechnology Total number of lectures: 45 Maximum Marks: 100The question paper should be divided into section A and section B. Total of 8 questions, 4 from section A and 4 from section B are to be set. The students will be required to attempt 5 questions selecting at least 2 from each section

Section ACell nanostructure interactions: Surface patterning to control the adhesion of cells using nanoscale topography and chemical modifications

Magnetosomes : Magnetotactic bacteria, Magnetite crystals, Magneto-aerotaxis, Applications Nanotherapeutics: Nanoshells, Dendrimers , Nanoparticles

Tissue engineering: Significance, Methodology, Tissue engineering scaffolds-

composition, properties, fabrication, cell seeding and proliferation

Nanomembranes: Freely suspended nanomembranes

Section BBionanodevices: Nanosensors and Actuators Nanopumps: Fabrication using SOI wafers Nonorobots: Developing nanorobots for biomedical applicationMolecular motors: Surface patterning and the control of motility of the actin/myosin motor system

Engineered nanopores: Classes of nanopores, engineering techniques, potential applications of nanopores

45

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http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=John%20%20Ingham/104-8398022-5365527

Reference Books

1. David S. Goodsell. Bionanotechnology: Lessons from Nature. (1st Edition) Wiley-Liss, 2004.2. Bharat Bhushan. Handbook of Nanotechnology (1st Edition). Springer Verlag, 2004. 3. Christof M. Niemeyer & Chad A. Mirkin (Eds.). Nanobiotechnology : Concepts,

Applications and Perspectives, 2004.

ELECTIVE - 1

BIO 805 Microbial Biodiversity



Section A

Microbial biodiversity: definition and introduction, evolution and diversity of microorganisms.

(7) Physiological and metabolic diversity of microorganisms.

(7) Microbial diversity of major ecosystems.

(8)

Section B

Biodiversity & role of microorganisms in plants and animal symbiosis. (8)

Microbial diversity in extreme environments. (9)

46

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Microbial biodiversity, biotechnology and future biodiversity. (6)

Reference Books1. Jo Handelsman. Biology Brought to Life (Student Version) McGraw-Hill, 2002.2. Oladele Ogunseitan. Microbial Diversity: Form and function in Prokaryotes.

Blackwell Publishers, 2004.3. 3. Alan T. Bull (Editor). Microbial Diversity and Bioprospecting. American

Society Microbiology, 2003.

47

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http://www.amazon.com/exec/obidos/search-handle-url/index=books&field-author=Oladele%20Ogunseitan/104-8398022-5365527

SCHEME OF EXAMINATION OF B.E. MBA INTEGRATED IN BIOTECHNOLOGY

Second Year - Third Semester



MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.


BIO301 Concepts in Bio-Technology-I 4+0 100 50 2 50 50 BIO351BIO302 Microbiology 4+0 100 50 3 50 50 BIO352BIO303 Biochemistry 4+0 100 50 3 50 50 BIO353BIO304 Cell & Molecular Biology 4+0 100 50 2 50 50 BIO354BIO306 Mathematics-III 3+1 100 50 -- -- -- --IBM 301 Organization Behavior 4+0 100 50 -- -- -- --WS357 Workshop Practice of Four

weeks after 2nd semester exams-- -- -- -- -- 50 --

Grand Total: 1350 24 600 300 10 200 250

Second Year - Fourth Semester



MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.


BIO401 Concepts in Biotechnology-II 4+0 100 50 2 50 50 BIO451IBM 401

Management of IT 4+0 100 50

BIO403 Chemical Reaction Engineering

4+0 100 50 2 50 50 BIO453

BIO404 Industrial Microbiology 4+0 100 50 2 50 50 BIO454BIO405 Immunology & Immuno-

technology4+0 100 50 2 50 50 BIO455

BIO406 Legal Laws and IPR 4+0 100 50 -- -- -- --

Grand Total: 1300 24 600 300 8 200 200

48

SCHEME OF EXAMINATION OF B.E. MBA INTEGRATED IN BIOTECHNOLOGY

Third Year - Fifth Semester



MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.


BIO501 Enzyme Engineering & Technology

4+0 100 50 2 50 50 BIO551

BIO502 Bio-Process Engineering 4+0 100 50 2 50 50 BIO552BIO503 Animal Cell Culture &

Biotechnology4+0 100 50 2 50 50 BIO553

BIO504 Transport Phenomena 4+0 100 50 2 50 50 BIO554IBM501

Marketing Management 3+1 100 50 -- -- -- --

IBM502

Human Resource Management

3+1 100 50 -- -- -- --

BIO506 Bioinstrumentation 3+1 100 50 -- -- -- --BIO507 Industrial Training (After 4th

Semester)-- -- -- -- 50 --

Grand Total: 1500 28 700 350 08 200 250

Third Year - Sixth Semester



MarksUni. Exam

Int.Ass.

Hours/Week

MarksUni.Exam

Int.Ass.


BIO601 Recombinant DNA Technology

4+0 100 50 2 50 50 BIO651

BIO602 Operation Research 3+1 100 50 2 50 50 BIO652BIO603 Introduction to Bio-

Informatics4+0 100 50 2 50 50 BIO653

BIO605 Down Stream Processing 4+0 100 50 3 50 50 BIO655BIO606 Biomaterials 4+0 100 50 -- -- -- --IBM601

Managerial Economics 3+1 100 50 -- -- -- --

49

IBM602

Corporate Legal Environment

3+1 100 50 -- -- -- --

BIO607 Training of 4 weeks after 6th semester exams: 50 Grand Total: 1500 28 700 350 9 200 250

SYLLABUS FORBE MBA Integrated (BIOTECHNOLOGY)

THIRD SEMESTERPaper Title: Organization Behavior (Theory)Paper Code: IBM 301 Max. Marks 100 Time: 3 HoursCourse Duration: 45 Lectures of one hour each.

Note: Examiner shall set eight questions, four from Part-A and four from Part-B of the syllabus. Candidate will be required to attempt any five questions selecting at least two questions from Part-A and two from Part-B.

Part AIntroduction to Organization Behavior [10]Definition and meaning of OB, impact of other sciences (Anthropology, Sociology, Psychology) on OB, perception, self esteem, attitude & personality, meaning of culture, impact of technology on OB.

Motivation, Learning & Leadership [13]Meaning of Motivation , Content theories of motivation (Maslows Hierarchy of needs ,Herzberg’s two factor theory ), Process theories ( Vroom’s Expectancy theory, Porter-Lawler Model ), Motivation applied (Job design , job rotation ,goal setting , MBO ), various methods of motivating employees, Behavioral & Cognitive theories of learning, Leadership theories (Trait theory, Fiedler’s Contingency theory ,Path –Goal leadership theory), Leadership styles (Blake & Mouton managerial grid, Hersey & Blanchard’s life cycle approach )

Part BGroup behavior: [10]Group Dynamics, conflict, power & politics ,Group behavior, types of groups ,group decision making , conflict in organizations and reason, interpersonal conflict, inter group conflict , meaning of power , classification of power , politics in organizations

Organization environment & Communication [12]Authority & responsibility ,delegation and division of work , quality of work life , communication process, modes of communication in organization and barriers to communication, formal & informal communication,

Recommended Books:

1. Organization Behavior – Stephen .P.Robbins , Prentice Hall of India2. Organization Behavior- Fred Luthans , Tata Mcgraw Hill

50


FOURTH SEMESTER

Paper Title: Management of Information Technology (Theory)Paper Code: IBM 401 Max. Marks 100 Time: 3 HoursCourse Duration: 45 Lectures of one hour each. Note: Examiner shall set eight questions, four from Part-A and four from Part-B of the syllabus. Candidate will be required to attempt any five questions selecting at least two questions from Part-A and two from Part-B.Part AInformation Technology (IT) [8]IT and society, IT infrastructure in India vis-à-vis developed nations (Telecommunication , Internet reach, PC, Broadband ,Mobile Phones ), IT applications in Healthcare & Education , meaning of E-Readiness and E-participation index as defined by United Nations, areas where growth is expected in future.

System Investigation & Analysis , Networking [8]System Analysis & Design , Symbols used in modeling a business process , Networking concepts, Ethernet ,IP addressing, Functioning of Routers, Bridges, hubs and switches in a network, Telecommunication (GSM, CDMA , Wireless and other new technologies)

Internet & Intranet [7]Functioning of Internet, Encryption & Digital signatures, Firewalls, Fraud on the Internet ,Virus , Hacking & Denial of Service attacks, Intellectual Property Protection on the Internet, Intranet & securityPart BE-Commerce & E-Governance [12]E-Commerce models , Intermediaries in E-Commerce, E-Governance in India ,study of successful E-Governance models like E-Choupal ,E-Payments (E-Cash, E-Wallets) and major players in the area, Online Shopping, Revenue models for Online Shopping Portals, Web Auctions like EBay, dealing with E-Waste.

Knowledge Management & Business Intelligence [10]Meaning of Knowledge Management, Designing a Knowledge Management System, Nature & Scope of Business Intelligence, Software for Business Intelligence, Data Warehousing and Data Mining techniques.Recommended Books:1. Data warehousing fundamentals – Paulraj Ponniah, John Wiley2. E-Governance : A comprehensive framework , D.N.Gupta, New Century Publications3 Business Data Communications & Networking , Jerry FitzGerald , Alan Dennis, John Wiley

51

4 Applied Data Communication:A Business-Oriented Approach, , James E.Goldman, John Wiley5.Information Technology for Management : Improving Performance in the Digital Economy , Efraim Turban , Linda Volonino , John Wiley.


FIFTH SEMESTER

Note: Syllabi of all other subjects given in scheme are same as syllabi of BE (Biotechnology) 5th semester.

Paper Title: Marketing ManagementPaper Code: IBM 501 Max. Marks 100 Time: 3 Hours



Objectives: (i) To understand the nature, tasks and the environment under which marketing operates. (ii) To study the theory, principles and practical aspects of various marketing functions. (iii) To learn to take marketing decisions.

Part AIntroduction to Marketing: [5]Definition; Scope and Importance of Marketing; Key Customer Markets; Concepts/Philosophies of Marketing; Holistic Marketing Concept; Marketing Tasks; Marketing Mix

Marketing Environment: [5]Marketing Environment; New Marketing Realities; New Consumer Capabilities; Demographic Environment; Social-Cultural Environment; Natural Environment; Technological Environment and Political-Legal Environment; SWOT analysis.

Analyzing Markets: [5]Marketing Research Process; Sources of data collection; factors influencing consumer behavior; buying decision process; post-purchase behavior; Organizational Buying; Stages in the Buying Process.

Market Segmentation: [6]Levels of market segmentation; segmenting consumer markets; Niche Marketing; segmenting business markets; Michael Porter’s five forces model; Analyzing competitors; strategies for market leaders; Targeting and Positioning.

Part B

52

Product Decisions: [6]Product characteristics; classifications; differentiation; packaging and labeling; Product Life Cycle.

Pricing Strategies: [6]Understanding Pricing; Setting the Price; Initiating and Responding to Price Changes; Reactions to Competitor’s Price Changes.

Marketing Channels: [6]Marketing Channels; Role of Marketing Channels; Identifying Major Channel Alternatives; Types of Intermediaries; Channel-Management Decisions, Retailing, Wholesaling.

Marketing Communication: [6]The Role of Marketing Communications; Communications Mix-Advertising, Sales Promotion, Public Relations and Publicity, Events and Experiences, Direct and Interactive Marketing, Personal Selling.

References:1. Kotler, P. Keller K.I. et al: Marketing Management, A South Asian Perspective.2. Ramaswamy, V.S. & Namakumari, S: Marketing management, planning,

implementation and control.3. Hepner H.W.: Modern Marketing- Dynamics and Management.

James M. Carman and Kenneth P. Phillips and Duncan: Marketing Principles and Methods.

4. Britt and Boyd (ed): Marketing Management and Administration.5. Cundiff, W.F. and Still R,: Basic Marketing.6. Converse Paul and Harvey W.Hugg: Elements of Marketing.7. Kotler, Philip and Armstrong: Principles of marketing, New Delhi, PHI, 19978. Hoffman, Douglas, K., et al: Marketing, Best Practices.9. Saxena, R: Marketing Management.

Paper Title: Human Resource ManagementPaper Code: IBM 502 Max. Marks 100 Time: 3 Hours

Course Duration: 45 Lectures of one hour each. Note: Examiner shall set eight questions, four from Part-A and four from Part-B of the syllabus. Candidate will be required to attempt any five questions selecting at least two questions from Part-A and two from Part-B.

Objectives: The objective of the paper is to make student aware of the various functions and importance of the HR department in any organization. It is basically concerned with managing the human resources, whereby the underlying objective is to attract retain and

53

motivate the human resources in any organization, which is the most challenging and daunting look for any organization today.

Part AIntroduction: [5]Meaning, scope, objectives and functions of HRM; Importance of Human Resource Management; HRM & HRD a comparative analysis;

Environment of HRM: [5]Role of government, internal and external forces; Human Resource Management practices in India.

Human Resource Planning: [5]Definition, objectives, process and importance; Job analysis, description, specification & job evaluation; Recruitment, selection, placement and induction process;

Human Resource Development: [6]Concept, Employee training & development; Career Planning & development; Promotions, demotions, transfers, separation, absenteeism & turnover;

Part BJob Compensation: [6]Wage & salary administration, incentive plans & fringe benefits.

Performance Management: [6]Concept & process, performance appraisal, Potential appraisal;

Quality of work life (QWL): [6]Meaning, techniques for improving QWL.

Industrial Relations: [6]Concept and theories, trade unions; Health, Safety & Employee welfare measures; Employee grievances and discipline, participation & empowerment; Introduction to collective bargaining.

References:1. V.S.P. Rao: Resources Management, Excel Publishing, New Delhi2. Edwin B. Flippo: Personnel Management, Tata McGraw Hill.3. Dale Yoder: Personnel Management & Industrial Relation4. Arun Monappa & Saiyadain: Personnel Management, Tata McGraw Hill5. Gray Dessler: Human Resource Management.

54


SIXTH SEMESTER

Note: Syllabi of all other subjects given in scheme are same as syllabi of BE (Biotechnology) 6th semester.

Paper Title: Managerial EconomicsPaper Code: IBM 601 Max. Marks 100 Time: 3 Hours



Objectives: To provide students with an understanding of basic economic principles of production & exchange-essential tools in making business decisions in today’s global economy. The object presents the foundation to understanding how the economy works, covering microeconomic description of business applications, including pricing for profit maximization, price elasticity, market structures and modeling of business in varying economic climates. The focus is on market economics, the organization that operation there and their business strategies.

Part AIntroduction to Managerial Economics: [4]Nature Scope and Importance of Managerial Economics. , opportunity costs , incremental principle , time perspective , discounts and equi marginal principles.

Demand Concepts and Analysis: [4]Individual Demand, Market Demand, Kinds of Demand, Determinants of Demand, Demand Functions, Functions, Demand Schedule and Law of Demand.

Theory of Consumer Behavior: [4]Cardinal Utility Approach and Ordinal Utility (Indifference Curves) Approach;

Elasticity of Demand: [4]Concept, Types, Measurement and importance.

Demand Forecasting: [5]

55

Sources of Data-Expert Opinions, Surveys and Market Experiments; Time Series Analysis-Trend Projection; Barometric Forecasting-Leading Indicators, Composite and diffusion Indices.

Part BProduction Function: [4]Concept and types, Returns to Factor and Returns to Scale, Law of Variable Proportions.

Cost concepts and Analysis: [4]Concept of Cost, Short run and Lung-run Cost Curves, Relationships among various costs, Break-even Analysis.

Revenue Curves: [4]Concept and Types.

Perfect Competition: [4]Characteristics, Equilibrium Price, Profit Maximizing output in Short Run and Long Run;

Monopoly: [4]Characteristics, Equilibrium Price, Profit Maximizing output in Short Run and Lung Run; Price Discrimination;

Imperfect Competition: [4]Monopolistic Competition, oligopoly and Barriers to Entry.

References:1. Craig Peterson, Lewis and Jain: Managerial Economics, Pearson Education2. Mark Hirshey: Managerial Economics, Thomson3. Dr. V.Panduranga Rao: Microeconomics-IBS Publication4. Keat: Economic Tools for Today’s Decision Makers, 4th Edition, Pearson

Education5. William Samuelson and Stephen G. Mark, Managerial Economics, 5 th Edition,

John Wiley & Sons.6. Managerial Economics Analysis: Problem Cases, 8th Edition, Truett & Truett,

Wiley7. K.K. Dewett: Modern Economic Theory, S. Chand & Sons, New Delhi8. Mote, Paul Gupta: Managerial Economics, Vikas Publisher, New Delhi9. A. Koutsoyiannis: Modern Microeconomics, Mc Millan, New Delhi10. R.L. Varshney & K. L. Maheshwari: Managerial Economics, S.Chand & Sons,

New Delhi

Paper Title: Corporate Legal Environment

56

Paper Code: IBM 602 Max. Marks 100 Time: 3 Hours



Objective: Corporate legal environment represents that external environment in which the organization has to work. The course covers the basic laws which a student must be aware of.Part AInformation Technology Act-2000 : [11]Objective of the act, documents excluded from the scope of the act, digital signatures, types of digital signatures in India, certifying authorities in India, regulation of certifying authorities, duties of subscribers, offences, appellate tribunal, penalties and adjudication

Company Law : [11]Definition and nature of a company, kinds of companies , formation of a company, memorandum of association, articles of association, prospectus, membership in a company, shares , transfer and transmission of shares, meetings and proceedings.

Part BPatents Law : [11]Patents Act 1970 as amended by the The Patents (Amendment) Act 2005, The Patents rules, 2003 as amended by The Patents (Amendment) Rules ,2006, Inventions not patentable, applications for patents, publication and examination of applications, grant of patents and rights conferred thereby, Patent Cooperation Treaty (PCT), Trade Related Intellectual Property Rights (TRIPS)

Consumer Protection Act 1986 : [12]Definitions under the act : complaint , consumer, defect, deficiency , unfair trade practice, consumer protection councils, redressal machinery under the act, district forum, state commission, national commission

References:1. Manish Arora , Guide to Patents Law, Universal Law Publishing Co2. K.C.Garg, V.K.Sareen , Mercantile Law, Kalyani Publishers

57

scheme syllb(3-8th sem)biotech bembacorrected

Documents

semester exams4

grand total

mba biotechnology

scheme of examination

simulation of bio

entrepreneurship modeling

bio802 bio803 bio804

energy balance calculations3