scientific publications - alifax...preparazione vetrini ifa altre tecnologie compatibili...
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Preparazione vetrini IFA
Altre tecnologie compatibili
Preparazione Blot di conferma
Screening automatizzato in IFA
Reattivi monodose in cartuccia
Dot blot tradizionali e microarray
IgGIgM IgA
Fino a 16 vetrini con lavaggio per singolo pozzetto
11 / 2
013
We Apply Science Multiplex, Customized Immunodot and Automation
www.d-tek.be
BlueDiver Instrument
A smart space saving instrument for the full automation of immunodot assays.
BlueDiver Combi
Immunodot Strips assembled by the user. The greatest fl exibility on the market in multiplexing diagnostic (up to 50 biomarkers can be custom-assembled).
Dr. DOT Interpretation System
A simple scanning device combined with an intuitive, user friendly Immunodot Interpretation software.
D-tek sa • Parc Initialis - Rue René Descartes 19 BE-7000 Mons BELGIUM • T. +32 (0)65 84 18 88 • F. +32 (0)65 84 26 63 • info @ d-tek.be www.d-tek.be
BlueDiver CombiKits
Class of Antibodies detected
Parameters detected
Connective Tissue Diseases
CENPA-24 IgG CENP-A
CENPB-24 IgG CENP-B
CENPAB-24 IgG CENP-A/B
DFS70-24 IgG DFS-70
DSDNA-24 IgG dsDNA
EJ-24 IgG EJ
HISTONES-24 IgG Histones
HMGCR-24 IgG HMGCR
JO1-24 IgG Jo-1
KU-24 IgG Ku
MDA5-24 IgG MDA-5
MI2-24 IgG Mi-2
NU-24 IgG Nucleosome
PCNA-24 IgG PCNA
PL12-24 IgG PL-12
PL7-24 IgG PL-7
PMSCL100-24 IgG PM/Scl 100
PMSCL75-24 IgG PM/Scl 75
RIBOP0-24 IgG Ribosome PO
RNAPOL3-24 IgG RNA Polymerase III (RPC155)
SCL70-24 IgG Scl-70
SM-24 IgG Sm
SMRNP-24 IgG Sm/RNP
SRP-24 IgG SRP
SSA60-24 IgG SSA/Ro60kD
SSA52-24 IgG Ro52kD (TRIM 21)
SSB-24 IgG SSB (La)
TIF1-24 IgG TIF1-γ
U1RNP-24 IgG U1-RNP (RNP68kD/A/C)
BlueDiver CombiKits
Class of Antibodies detected
Parameters detected
Vasculitis and Goodpasture Syndrome
GBM-24 IgG GBM
MPO-24 IgG MPO
PR3-24 IgG PR3
Autoimmune Liver Diseases
FACTIN-24 IgG F-actin
GP210-24 IgG gp210
LC1-24 IgG LC1
LKM1-24 IgG LKM1
M2NAT-24 IgG M2 native (nPDC)
M2REC-24 IgG M2 recombinant
M2BCOADC-24 IgG M2/BCOADC-E2
M2OGDC-24 IgG M2/OGDC-E2
M2PDC-24 IgG M2/PDC-E2
SLA-24 IgG SLA
SP100-24 IgG sp100
Autoimmune Gastritis
IF-24 IgG IF
PCA-24 IgG PCA
BlueDiver Combi Antigen Chips
11 / 2013
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CEN
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4Ig
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NP-A
CEN
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CENP
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CEN
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-24
IgG
CENP
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DFS
70-2
4Ig
GDF
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DSD
NA
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IgG
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NU
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me
PC
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IgG
PCNA
PL1
2-24
IgG
PL-1
2
PL7
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IgG
PL-7
PM
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4Ig
GPM
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100
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Ribo
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IgG
RNA
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SCL7
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IgG
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4Ig
GSm
SMR
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IgG
Sm/R
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SRP
-24
IgG
SRP
SSA
60-2
4Ig
GSS
A/Ro
60kD
SSA
52-2
4Ig
GRo
52kD
(TRI
M 21
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SSB
-24
IgG
SSB
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TIF1
-24
IgG
TIF1
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U1R
NP
-24
IgG
U1-R
NP (R
NP68
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M2O
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SLA
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IgG
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Ant
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TEST DOTMICROARRAYCOMPONIBILI
ANTIGENI CHIP
CONTROLLO POSITIVOIN TRIPLICATO
ANTIGENEIN TRIPLICATO
CONTROLLO NEGATIVOIN TRIPLICATO
11 / 2
013
We Apply Science Multiplex, Customized Immunodot and Automation
www.d-tek.be
BlueDiver Instrument
A smart space saving instrument for the full automation of immunodot assays.
BlueDiver Combi
Immunodot Strips assembled by the user. The greatest fl exibility on the market in multiplexing diagnostic (up to 50 biomarkers can be custom-assembled).
Dr. DOT Interpretation System
A simple scanning device combined with an intuitive, user friendly Immunodot Interpretation software.
D-tek sa • Parc Initialis - Rue René Descartes 19 BE-7000 Mons BELGIUM • T. +32 (0)65 84 18 88 • F. +32 (0)65 84 26 63 • info @ d-tek.be www.d-tek.be
BlueDiver CombiKits
Class of Antibodies detected
Parameters detected
Connective Tissue Diseases
CENPA-24 IgG CENP-A
CENPB-24 IgG CENP-B
CENPAB-24 IgG CENP-A/B
DFS70-24 IgG DFS-70
DSDNA-24 IgG dsDNA
EJ-24 IgG EJ
HISTONES-24 IgG Histones
HMGCR-24 IgG HMGCR
JO1-24 IgG Jo-1
KU-24 IgG Ku
MDA5-24 IgG MDA-5
MI2-24 IgG Mi-2
NU-24 IgG Nucleosome
PCNA-24 IgG PCNA
PL12-24 IgG PL-12
PL7-24 IgG PL-7
PMSCL100-24 IgG PM/Scl 100
PMSCL75-24 IgG PM/Scl 75
RIBOP0-24 IgG Ribosome PO
RNAPOL3-24 IgG RNA Polymerase III (RPC155)
SCL70-24 IgG Scl-70
SM-24 IgG Sm
SMRNP-24 IgG Sm/RNP
SRP-24 IgG SRP
SSA60-24 IgG SSA/Ro60kD
SSA52-24 IgG Ro52kD (TRIM 21)
SSB-24 IgG SSB (La)
TIF1-24 IgG TIF1-γ
U1RNP-24 IgG U1-RNP (RNP68kD/A/C)
BlueDiver CombiKits
Class of Antibodies detected
Parameters detected
Vasculitis and Goodpasture Syndrome
GBM-24 IgG GBM
MPO-24 IgG MPO
PR3-24 IgG PR3
Autoimmune Liver Diseases
FACTIN-24 IgG F-actin
GP210-24 IgG gp210
LC1-24 IgG LC1
LKM1-24 IgG LKM1
M2NAT-24 IgG M2 native (nPDC)
M2REC-24 IgG M2 recombinant
M2BCOADC-24 IgG M2/BCOADC-E2
M2OGDC-24 IgG M2/OGDC-E2
M2PDC-24 IgG M2/PDC-E2
SLA-24 IgG SLA
SP100-24 IgG sp100
Autoimmune Gastritis
IF-24 IgG IF
PCA-24 IgG PCA
BlueDiver Combi Antigen Chips
Le strip-test sono facilmente componibili e personalizzabili con 6 differenti “antigeni chip” disponibili in cartucce da 24
Ogni chip comprende i dot antigene, il controllo positivo e negativo spottati in triplicato su membrana di nitrocellulosa con barcode identificativo
Le cartucce reattivi sono uniche per tutti i marker (kit da 24 )
Sono disponibili 50 differenti pannelli in formato microchip
La lettura delle strip è effettuata con scanner dedicato e l’interpretazione con il software d’analisi Dr. Dot
Test Dot Microarray componibili per il sistema automatico BlueDiver
ANTIGENI CHIP
CONTROLLO POSITIVO IN TRIPLICATO
CONTROLLO NEGATIVO IN TRIPLICATO
ANTIGENE SPOTTATO IN TRIPLICATO
Le strip-test sono facilmente componibili e personalizzabili con 6 differenti “antigeni chip” in cartucce da 24
Ogni chip è composta da dot antigene, controllo positivo e negativo spottati in triplicato su membrana di nitrocellulosa con barcode identificativo
Le cartucce di reattivi sono universali e abbinabili a tutti i marker (kit da 24 )
Sono disponibili 50 differenti analiti in formato microchip
La lettura delle strip è effettuata con scanner dedicato e l’interpretazione con il software d’analisi Dr. Dot
Il pannello Line Blot per la diagnosi di neuropatie autoimmuni si compone di kit automatizzabili sul sistema Blue Diver. Il loro utilizzo permette di eseguire una diagnosi con elevata sensibilità e specificità e di fornire informazioni fondamentali per la terapia.
PNS9, PNS11 e PNS14 DIVER BLOT IgG sono kit line blot prodotti da Ravo Diagnostika per la ricerca su siero, plasma e liquor di anticorpi contro antigeni onconeuronali (ONA).
GANGLIOSIDI e SULFATIDI LINE BLOT sono kit line blot prodotti da Generic Assays per lo screening o per la determinazione semi-quantitativa degli anticorpi di classe IgG o IgM diretti verso 12 diversi antigeni (gangliosidi e sulfatidi)
LINE BLOT PER LA DIAGNOSI DI NEUROPATIE AUTOIMMUNI
GESTIONE INTEGRATA PER L’AUTOMAZIONE COMPLETA NELSETTORE DELL’AUTOIMMUNITÀ
Sistema per la gestione globale del laboratorio
Sistema automatico per test Dot Autoimmunità
ALIFAX S.r.l.
Via Petrarca, 2/1 35020 - Polverara (PD) - Italy - www.alifax.com - [email protected] - Tel: +39 049 0992000
Alifax Entered in Padova companies register at the VAT n. 04337640280. Company stock € 10.000.000 entirely deposited.
Company with single partner and subjected to direction and coordination of Alifax Holding S.p.A.
Rev
. EN
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6
SCIENTIFIC PUBLICATIONSNEW ESR SYSTEM GENERATION
TEST 1 is the only analyzer capable to give ESR results in 20 seconds by measuring the kinetic of red cells aggregation. Alifax technology is recognized by CLSI guidelines as an alternative method for ESR and included in the most prestigious External Quality Assurance and Proficiency Testing programs.
*Clinical Laboratory and Standard Institute (formerly NCCLS) Procedures for the Erythrocyte Sedimentation Rate Test; Approved Standard - Fifth Edition H02-A5, Vol.31 No.11 2011
EACH SAMPLE IS READ 1000 TIMES IN 20 SECONDS
SEDIMENTATION ESR CAPILLARY PHOTOMETRY
Temperature variability Temperature control 37°C
Hematocrit influence Independent from Hematocrit value
Dilution problems using Sodium Citrate No dilution, use of EDTA tube
Inadequate materials and pipettes variability Use of the same capillary for all samples
Vibrations and pipettes verticality No influence of vibration or other external factors
Not standardized sample mixing Automated mixing step
Controls and Calibrators lack Latex Controls and Calibrators available
Poor reproducibility High reproducibility
20’’
ESR IN 20 SECONDS
1. G. Scott, T. Nguyen, S. Leunda Ostolaza, C. Galiano, G. Nalbandian and B. Miller (Iris Diagnostics, Chatsworth, CA) “Roller 20PN and Westergren Correlation” White Paper 2012 Chatsworth, CA.
2. CH Cha, CJ Park, YJ Cha, HK Kim, DH Kim, Honghoon, JH Bae, JS Jung, S Jang, HS Chi, DS Lee, HI Cho (Department of Laboratory Medicine, University of Ulsan College of Medicine and Gangneung Asan Hospital, Gan- gneung, Korea) “Erythrocyte Sedimentation Rate Measurements by TEST 1 Better Reflect Inflammation Than Do Those by the Westergren Method in Patients With Malignancy, Autoimmune Disease, or Infection” Am J Clin Pathol. 2009 Feb;131(2):189-94.
3. B Frollano, G Cigliana, G Vitelli, R Fontinovo, S Giommi, I Cordone (Clinical Pathology, Regina Elena Cancer Institute, IFO, Rome – Italy) “Capillary Erythrocyte Sedimentation Rate (ESR) in oncological patients: low haematocrit pitfalls and sample collection optimization in a certified quality system laboratory” SIBioC National Congress 28-31 October 2008, Rimini, Italy.
4. R. Pajola, E. Piva, B. Robecchi, F. Tosato, M. Plebani. (Dep. of Laboratory Medicine, Padua University School of Medicine, Padua, Italy) “The Erythrocyte Sedimentation Rate (ESR): an old test with new contents” SIBioC National Congress 28-31 October 2008, Rimini, Italy.
5. J Reis, J Diamantino, N Cunha, F Valido (Clinical Pathology Department, IPO Coimbra; Francisco Gentil, EPE, Portugal),“Erythrocyte sedimentation rate in blood a comparison of theTest 1 ESR system with the ICSH reference method. Clinical Chemistry and Laboratory Medicine 2007 June; 45, Special Supplement, p.S118, MO77.
6. E Piva, R Pajola, V Temporin, M Plebani (Dept. of Laboratory Medicine, University-Hospital, Padova, Italy) “A new turbidimetric standard to improve the quality assurance of the erythrocyte sedimentation rate measurement” Clinical Biochemistry 2007 Apr; 40(7):491-5. Epub 2007 Jan 8.
7. Arikan S, Akalin N. (Biochemistry Department, Baskent University, Ankara, Turkey) “Comparison of the erythrocyte sedimentation rate measured by the Micro Test 1 sedimentation analyzer and the conventional Westergren method” Ann Saudi Med 2007; 27(5): 362-365.
8. LY Li, WB Chen, G Feng, SF Shen (Dep of Clinical Laboratory, Sixth People’s Hospital Affiliated to Shangai Jiaotong University, Shangai 200233, China) “Evaluation of the Microtest 1 ESR analyzer and investigation of the reference value” Chin J Lab Med, March 2007, Vol 30, N 3 (article in Chinese).
9. S Ozdem, HS Akbas, L Donmez, M Gultekin (Clinical Biochemistry Unit, Medical Faculty, Central Laboratory, Akde- niz University, Antalya,Turkey) Comparison of TEST 1 with SRS 100 and ICSH reference method for the measurement of the length of sedimentation reaction in blood. Clin Chem Lab Med. 2006;44(4):407-12.
10. NE Ajubi, AJ Bakker, GA van den Berg (Stichting KCL, Dept. of Clinical Chemistry, Leeuwarden, The Netherlan- ds), “Determination of the length of sedimentation reaction in blood using the Test 1 system: comparison with the Sedimatic 100 method, turbidimetric fibrinogen levels and the influence of M proteins” Clin Chem Lab Med 2006, 44 (7): 904-906.
11. Y Kagawa, N Ikeda, S Ito, S Makino, N Miyake (Clinical Test Section of Eiju Hospital, Finggal Link Co. and Dept. of Clinical Pathology of Juntendo University),“Evaluation for ESR automated measuring instrument with EDTA”, 36th Japan Society for Clinical Laboratory Automation, 30 September 2004, Japan.
12. B Rosas, P Díaz, C Musa, J Aldunate (Servicio Laboratorio Clínico, Hospital Universidad de Chile),“Estudio Comparativo de 2 equipos que realizan VHS, Test1 y Vesmatic”, XII Congreso Chileno de Tecnologia Medica, 20–22 October 2004, Santiago, Chile (article in Spanish).
13. M Plebani, P D’Altoé, V Temporin, E Piva, M Buttarello, M Sanzari, (Dept. of Laboratory Medicine, University- Hospital, Padova, Italy),“Variabilità Biologica Intra ed Interindividuale della Velocità di Eritrosedimentazione”, 36th SIBioC, 8-11 June 2004, Padova, Italy (article in Italian).
14. E Melkič, M Piskar, P Lenart (Klinični center Ljubljana, Klinični intitut za klinično kemijo in biokemijo) “Nov način merjenja hitrosti sedimentacije eritrocitov z analizatorjem Test1 Alifax”, 2 Kongres Hematologov in Transfuziologov Slovenije z Mednarodno UbeleÏbo, 23–24 April 2004, Portoroz, Slovenia (article in Slovenian).
15. B Olivera Alonso, M Sirvent Monerris, MT Rotella Belda,V Ballenilla Antón, G Vidal (M Laboratorio Hospital San Vicente y Area Sanitaria 18.Alicante, Spain),“Cambio De Método Para La Determinación De V.S.G.: Repercusiones Sobre La Fase Preanalítica”, Generalitat Valenciana - Conselleria De Sanitat (for Valencia Government – MOH), Spain 2004 (poster in Spanish).
16. P Galiano, “Quality and Automation in the Determination of the Erythrocyte Sedimentation Rate”, Symposium 046, 22nd World Congress of Pathology & Laboratory Medicine, 30 August- September 2003, Busan, Korea.
17. M Nicoli, E Lanzoni, A Massocco, (Laboratory of Clinical Chemistry and Haematological Analysis, Ospedale Civile Maggiore,Verona, Italy) “Integrated Haematology and Coagulation Laboratory”, Poster, Euromedlab Congress, 1-5 June 2003, Barcelona, Spain.
18. M Plebani (Dept. of Laboratory Medicine, University-Hospital, Padova, Italy), “Erythrocyte Sedimentation Rate: Innovative Techniques for an Obsolete Test?”, Clinical Chemistry and Laboratory Medicine, 2003, 41 (2): 115-116.
19. A Romero, M Muñoz, G Ramirez (Dept. of Haematology, H.C.U. “Virgen de la Victoria”, Málaga & *GIEMSA, School of Medicine, University of Málaga, Spain), “Determination of the Length of Sedimentation Reaction in Blood: a Comparison of the Test1 ESR System with the ICSH Reference Method and the Sedisystem”, Clinical Chemistry and Laboratory Medicine 2003, 41 (2).
20. D Giavarina, S Capuzzo, F Cauduro, M Carta, G Soffiati (Clin. Chem. & Hematol. Lab., San Bortolo Hospital, Vicenza, Italy), “Internal Quality Control for Erythrocyte Sedimentation Rate Measured Test 1 Analyzer” Clinical La- boratory 2002, 48:459-462.
21. E Heverin (Galway-Mayo Institute of Technology, Ireland), “Comparison of the Westergren method versus the TEST1 technique for determining the Erythrocyte Sedimentation Rate” May 2002, private communication.
22. BH Lee, J Choi, MS Gee, KK Lee, H Park (Dept. of Laboratory Medicine,Kangbuk Samsung Hospital, Sungkyun- kwan University School of Medicine, Seoul, Korea), “Basic Evaluation and Reference Range Assessment of TEST1 for the Automated Erythrocyte Sedimentatioon Rate” Journal of Clinical Pathology and Quality Control, Vol. 24, No. 1, 2002 (article in Korean).
23. E Piva, P Fassina, M Plebani (Dept. of Laboratory Medicine, University-Hospital, Padova, Italy), “Determination of the length of sedimentation reaction (erythrocyte sedimentation rate) in non-anticoagulated blood with the Microtest 1” Clin Chem Lab Med. 2002 Jul;40(7):713-7.
24. M Plebani, E Piva (Dept. of Laboratory Medicine, University-Hospital, Padova, Italy), “Erythrocyte Sedimentation Rate. Use of Fresh Blood for Quality Control”, American Journal of Clinical Pathology, 2002, 117:621-626.
25. D Smith D Spedding, (Dade Behring Diagnostics, New Zealand), “Evaluation of Agreement between the TEST1 and Starrsed Automated ESR Analysers”, November 2001, private communication.
26. D Giavarina, S Capuzzo, M Carta, F Cauduro, G Soffiati (Clin. Chem. & Hematol. Lab., San Bortolo Hospital, Vicenza, Italy), “Internal Quality Control for Erythrocyte Sedimentation Rate (ESR) measured by TEST-1 Analyzer”, Clinical Chemistry, June 2001, 47: 162.
27. E Piva, MC Sanzari, G Servidio M Plebani (Dept. of Laboratory Medicine, University-Hospital, Padova, Italy), “Length of Sedimentation Reaction in Undiluted Blood (Erythrocyte Sedimentation Rate): Variations with Sex and Age and Reference Limits”, Clinical Chemistry and Laboratory Medicine, May 2001, 39: 451-454.
28. N de Jonge, I Sewkaransing, J Slinger, JJM Rijsdijk (Dept. Clinical Chemistry, Leyenburg Hospital, The Netherlan- ds) Erythrocyte Sedimentation Rate by Test-1 Analyzer Clinical Chemistry, June 2000, 46: 881-882.
29. M Plebani, S De Toni, MC Sanzari, D Bernardi, E Stockreiter (Department of Laboratory Medicine, University- Hospital of Padua, Italy) The TEST 1 automated system: a new method for measuring the erythrocyte sedimentation rate. Am J Clin Pathol. 1998 Sep;110(3):334-40.
30. G Soffiati (Clinical Chemistry and Hematology Laboratory, San Bortolo Hospital, Vicenza, Italy), “Nuovo Metodo per la Determinazione della Velocità di Eritrosedimentazione (VES)”, August 1998, private communication.
31. N Cirilli, Z Abu Asy, N Giacchè, F Bordicchia, S Paolucci, M Tocchini (Dept. of Laboratory Medicine, G. Salesi Hospital, Ancona, Italy), “TEST1: Un Nuovo Metodo per la Determinazione della VES”, Biochimica Clinica, Vol. 22, N. 5-6, 1998, p. 339.
Due to regulatory requirements, the products listed herein are available for sale in any relevant Country territory provided that they have been registered according to the applicable domestic law. The products listed herein may have incurred changes with respect to the last available edition.
TEST 1 CAPILLARY PHOTOMETRY TECHNOLOGY overcomes the variables and limitations of the sedimentation method also listed in the CLSI document*.
Aggregation after 20 seconds
Rev
. 23.
04.1
8
ANTIGENI IgG - Chip CODICE PRODOTTO ANTIGENI IgG - Chip CODICE PRODOTTOBCOADC (040) AL AD BCOADCC OJ (049) AL AD OJCCentromer A (047) AL AD CENTAC PCA (Cellule parietali) (043) AL AD PCACCentromer B (048) AL AD CENTBC PCNA (012) AL AD PCNACCentromer A+B (011) AL AD CENTABC PDC (039) AL AD PDCCDFS-70 (026) AL AD DFS70C PL-7 (015) AL AD PL7CdsDNA (022) AL AD DSDNAC PL-12 (016) AL AD PL12CEJ (018) AL AD EJC PMSCL-75 (009) AL AD PMSCL75CF Actin (038) AL AD FACTINC PMSCL-100 (008) AL AD PMSCL100CGAD I (045) AL AD GADIC PR3 (029) AL AD PR3CGAD II (046) AL AD GADIIC Ribosome P0 (013) AL AD RIBOCGBM (030) AL AD GBMC RNA Polymerase III (025) AL AD RNACGliadin (044) AL AD GLIADINC Scl-70 (007) AL AD SCL70CGP210 (033) AL AD GP210C SLA (037) AL AD SLACHA (052) AL AD HAC Sm (001) AL AD SMCHistones (023) AL AD HISTC Sm/RNP (003) AL AD SMRNPCIntrinsic Factor (042) AL AD IFC Sp100 (034) AL AD SP100CJO1 (006) AL AD JO1C SRP (017) AL AD SRPCKu (010) AL AD KUC SSA/Ro 52kD TRIM (024) AL AD SSA52CKS (050) AL AD KSC SSA/Ro 60kD (004) AL AD SSA60CLC1 (Liver Cytosol 1) (036) AL AD LC1C SSB/La (005) AL AD SSBCLKM1 (035) AL AD LKM1C Th/To (053) AL AD THTOCM2 native (031) AL AD M2NATC TIF1-Ɣ (020) AL AD TIFCM2 Recombinante (032) AL AD M2RECC U1-RNP (002) AL AD U1RNPCMDA5 (019) AL AD MDA5C ZO (051) AL AD ZOCMi-2 (014) AL AD MI2CMPO (028) AL AD MPOC CARTUCCE UNIVERSALINucleosome (021) AL AD NUC 24 cartucce
+ 24 rack per strip AL AD CARTC
OGDC (041) AL AD OGDCC
NEUROPATIE AUTOIMMUNI
GANGLIOSIDI E SULFATIDI LINE BLOT IgM Sulfatidi, GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, GQ1b GA 50391
GANGLIOSIDI E SULFATIDI LINE BLOT IgG Sulfatidi, GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, GQ1b GA 50381
GANGLIOSIDI E SULFATIDI LINE BLOT IgG e IgM Sulfatidi, GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, GQ1b GA 50301
ONCONEURALI PROFILE IgG - 9 Ag GAD65, HuD, Yo, Ri, CV2 (CRMP5), Anfifisina, Ma1, Ma2, SOX1 RAV PNS9DIV-24
ONCONEURALI PROFILE IgG - 11 Ag GAD65, HuD, Yo, Ri, CV2 (CRMP5), Anfifisina, Ma1, Ma2, SOX1, Tr, Zic4 RAV PNS11DIV-24
ONCONEURALI PROFILE IgG - 14 Ag GAD65, HuD, Yo, Ri, CV2 (CRMP5), Anfifisina, Ma1, Ma2, SOX1, Tr, Zic4, Titina, Recoverina, PKC ɣ
RAV PNS14DIV-24*
*di prossima introduzione
PROFILI DOT
INGOMBRO RIDOTTOStrumento leggero e compatto
AMPIO RANGE DI TESTDisponibili oltre 40 pannelli di diversi profili antigenici
REAGENTI MONODOSE IN CARTUCCE PRONTE ALL’USONessuno sprecoNessuna contaminazioneNessun refluo di scarico
FACILE E INTUITIVOProcedura di caricamento e avvio semplice, intuitiva e guidata via touch-screen in pochi passaggi
FLESSIBILEPer ogni seduta d’analisi è possibile testare da 1 a 24 campioni con differenti profili di analisi
RAPIDOL’agitazione meccanica per immersione delle strip nei diversi reagenti permette di accelerare i tempi di reazione riducendo i tempi di analisi a solo 1 ora
INTERPRETAZIONE STANDARDIZZATA DEI RISULTATILa lettura e l’interpretazione delle strip sono effettuate con scanner e software d’analisi dedicati
TRACCIABILITA’L’identificazione e la tracciabilità sono garantite dal barcode presente sia su strip che su cartuccia
SPECIFICHE TECNICHEDimensioni: 515 x 298 x 289 mmPeso: 16 KgTensione alimentazione: 110/240 VACPotenza nominale:100 WattFrequenza: 50 or 60 ± 2 HzTemperatura ambientale: +10÷30 °C
CARATTERISTICHE DEI DOT BLUE DIVER TRADIZIONALI
TEST MULTIPLII dot tradizionali permettono l’analisi simultanea fino a 12 autoanticorpi diversi sulla stessa membrana. E’ disponibile un ampio spettro di combinazioni di antigeni patologia-specifici
ELEVATA QUALITA’ DEGLI ANTIGENIOgni strip di nitrocellulosa è rivestita con antigeni target purificati e/o ricombinanti di elevata qualità: sono presenti un controllo positivo interno per confermare la corretta esecuzione del test e un controllo negativo che funge da cut-off interno
VOLUMI MINIMI DI CAMPIONESono necessari solo 10 µl di siero non diluito
CARATTERISTICHE DEL TEST DOT BLUE DIVERMICROARRAY
TEST MULTIPLIIl formato microarray permette l’analisi simultanea fino a 25 autoanticorpi diversi sulla stessa membrana
RISULTATO QUANTITATIVOI risultati quantitativi vengono calcolati sulla base di una curva di calibrazione a 6 punti incluso il bianco
ANTIGENI IN TRIPLICATOCiascun antigene è deposto in triplo dot sulla membrana per determinare la precisione del risultato e la variabilità intrasaggio
VALIDAZIONE AUTOMATICA DEI TESTControlli multipli permettono la validazione della procedura: dal corretto caricamento dei campioni alla corretta cinetica di coniugato e substrato
VOLUMI MINIMI DI CAMPIONESono necessari solo 10 µl di siero non diluito
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We Apply Science Multiplex, Customized Immunodot and Automation
www.d-tek.be
BlueDiver Instrument
A smart space saving instrument for the full automation of immunodot assays.
BlueDiver Combi
Immunodot Strips assembled by the user. The greatest fl exibility on the market in multiplexing diagnostic (up to 50 biomarkers can be custom-assembled).
Dr. DOT Interpretation System
A simple scanning device combined with an intuitive, user friendly Immunodot Interpretation software.
D-tek sa • Parc Initialis - Rue René Descartes 19 BE-7000 Mons BELGIUM • T. +32 (0)65 84 18 88 • F. +32 (0)65 84 26 63 • info @ d-tek.be www.d-tek.be
BlueDiver CombiKits
Class of Antibodies detected
Parameters detected
Connective Tissue Diseases
CENPA-24 IgG CENP-A
CENPB-24 IgG CENP-B
CENPAB-24 IgG CENP-A/B
DFS70-24 IgG DFS-70
DSDNA-24 IgG dsDNA
EJ-24 IgG EJ
HISTONES-24 IgG Histones
HMGCR-24 IgG HMGCR
JO1-24 IgG Jo-1
KU-24 IgG Ku
MDA5-24 IgG MDA-5
MI2-24 IgG Mi-2
NU-24 IgG Nucleosome
PCNA-24 IgG PCNA
PL12-24 IgG PL-12
PL7-24 IgG PL-7
PMSCL100-24 IgG PM/Scl 100
PMSCL75-24 IgG PM/Scl 75
RIBOP0-24 IgG Ribosome PO
RNAPOL3-24 IgG RNA Polymerase III (RPC155)
SCL70-24 IgG Scl-70
SM-24 IgG Sm
SMRNP-24 IgG Sm/RNP
SRP-24 IgG SRP
SSA60-24 IgG SSA/Ro60kD
SSA52-24 IgG Ro52kD (TRIM 21)
SSB-24 IgG SSB (La)
TIF1-24 IgG TIF1-γ
U1RNP-24 IgG U1-RNP (RNP68kD/A/C)
BlueDiver CombiKits
Class of Antibodies detected
Parameters detected
Vasculitis and Goodpasture Syndrome
GBM-24 IgG GBM
MPO-24 IgG MPO
PR3-24 IgG PR3
Autoimmune Liver Diseases
FACTIN-24 IgG F-actin
GP210-24 IgG gp210
LC1-24 IgG LC1
LKM1-24 IgG LKM1
M2NAT-24 IgG M2 native (nPDC)
M2REC-24 IgG M2 recombinant
M2BCOADC-24 IgG M2/BCOADC-E2
M2OGDC-24 IgG M2/OGDC-E2
M2PDC-24 IgG M2/PDC-E2
SLA-24 IgG SLA
SP100-24 IgG sp100
Autoimmune Gastritis
IF-24 IgG IF
PCA-24 IgG PCA
BlueDiver Combi Antigen Chips
Sistema Blue Diver
Dot Diver 12 Profile
Cartucce pronte all’uso contenenti tutti i reagenti necessari
Software Dr. Dot
IgGIgM IgA
Dot Diver Quantrix
SISTEMA COMPATTO PER L’AUTOMAZIONEDI TEST DOT SU MEMBRANA
PATOLOGIE DEL TESSUTO CONNETIVO Codice Prodotto
ANA - DFS70 - 12 Ag Sm, RNP, Sm/RNP, SSa-60kD, SSb, Scl 70, PMScl-100, Ku, CENP A/B, PCNA, Mi2, DFS70 AL AD-ANADFDBD
ANA 12 Ag R DOT Sm, U1-RNP, Sm/RNP, SSA/Ro 60kD, Ro 52kD, SSB, Jo1, Scl-70, PM-Scl 100, CENP-A/B, PCNA , DFS-70 AL AD-ANA12RDBD
ANA-Mi2 - 12 Ag Sm, Sm/RNP, RNP (68 kD+A+C), SSa, SSb, Jo1, Scl70, Pm/ Scl, Ku, CENP A/B, PCNA, Mi2 AL AD-ANAMIDBD
DotDiver ANA - 8 Ag Sm, Sm/RNP, SSA, SSB, Jo-1, Scl-70, PMScl-100, CENP-A/B GA 5016
CONNECTIVITIS PROFILE - 10 Ag Nucleosomi, dsDNA, Istoni, Sm, Sm/RNP, SSA, Ro 52 kDa, SSB, Jo1, Scl70 AL AD-CP10DBD
CYTOPLASMATIC PROFILE - 6 Ag M2 Complex, JO1, PL7, PL12, Ribosomi, SRP (Signal Recognition Particle) AL AD-CYTO6DBD
ENA - 10 Ag Sm, Sm/RNP, SSA, SSB, Jo1, Scl70, PMScl, Ku, CENP A/B, PCNA AL AD-ENA10DBD
ENA - 6 Ag Sm, Sm/RNP, SSA, SSb, Jo1, Scl 70 AL AD-ENADBD
ENA 6 PROFILE-NUCL. - 7 Ag Sm, Sm/RNP, SSA, SSB, Jo1, Scl 70, Nucleosomi AL AD-ENDBD
ENA/ANA - 12 Ag Sm, Sm/RNP, RNP (68 kD + A + C), SSA, SSB, Jo1, Scl70, PMScl, Ku, CENP A/B, PCNA, Ribosomi (P0, P1, P2) AL AD-FULLANADBD
ENA-NUCLEOSOMI-ISTONI D - Ag 8 Sm, Sm/RNP, SSA, SSB, Jo1, Scl 70, Nucleosomi, Istoni AL AD-NHEDBD
LUPUS DOT-BLOT - 4 Ag Nucleosomi, Istoni, Sm, Rib P0 AL AD-LUDBD
MYOSITIS PROFILE 12-OJ A IgG DOT Jo-1, PL-7, PL-12, EJ, OJ, SRP, Mi-2, MDA-5, TIF-1Ɣ, SAE1, SAE2, NXP-2 AL AD-MYOSO12ADBD
MYOSITIS PROFILE 12 A IgG DOT Jo-1, PL-7, PL-12, EJ, SRP, Mi-2, MDA-5, TIF1-γ, SSA/Ro52kD, SAE1, SAE2, NXP-2 AL AD-MYOS12ADBD
NDH CHROMATIN - 3 Ag Nucleosomi, dsDNA, mix Istoni (H1, H2a, H2b, H3-H4 da timo bovino) AL AD-NDHDBD
NUCLEOSOMI-ISTONI - 2 Ag Nucleosomi, Istoni AL AD-HNDBD
SCLERODERMA PROFILE - 8 Ag Scl 70, CENP-A, CENP-B, PMScl-100, PM Scl-75, Ku, RNA Polimerasi III, U1-RNP AL AD-SCLERO8DBD
SCLERODERMA PROFILE - 10 Ag Scl 70, CENP-A, CENP-B, PMScl-100, PM Scl-75, Ku, RNA Polimerasi III, U1-RNP, Th/To, Fibrillarina AL AD-SCLERO10DBD
SCLERODERMA PROFILE - 12 Ag* Scl 70, CENP-A, CENP-B, PMScl-100, PM Scl-75, Ku, RNA Polimerasi III, U1-RNP, Th/To, Fibrillarina, NOR-90, NOR-90, Ro 52kD
AL AD-SCLERO12DBD
SCLERO POLY SYNTHETASE PROFILE - 12 Ag Jo1, PL7, PL12, EJ, SRP, Mi2, MDA-5, TIF-y, Ku, PMScl, Scl70, SSA/Ro 52Kd AL AD-SPS12DBD
SCLERO POLY SYNTHETASE PROFILE - 8 Ag Jo1, PL7, PL12, Mi2, Ku, PMScl, Scl70, SRP (Signal Recognition Particle) AL AD-SPS8DBD
SYNTHETASE PROFILE - 10 Ag J01, PL-7, PL-12, EJ, OJ, KS, ZO, HA, SRP, Rib P0 AL AD-SYNTHE10DBD
VASCULITI E SINDROME DI GOODPASTURE
GBM GOODPASTURE - 1 Ag GBM AL AD-GBMDBD
DotDiver ANCA - 3Ag MPO, PR3, GBM GA 5018
VASCULAR MPO-PR3 - 2Ag MPO, PR3 AL AD-MPDBD
EPATITI AUTOIMMUNI E CIRROSI BILIARE
ANTI-MITOCONDRI PROFILE - 2 Ag M2/nPDC, mix (OGDC-E2, BCOADC-E2, PDC-E2) AL AD-MI2DBD
ANTI-MITOCONDRI PROFILE - 4 Ag M2/nPDC, OGDC-E2, BCOADC-E2, PDC-E2 AL AD-M4RDBD
LIVER PROFILE - 10 Ag M2/nat.PDC, M2/0GDC-E2, M2/BC0ADC-E2, M2/PDC-E2, LKMI, LC1, SLA, F-Actina, gp210, Sp100 AL AD-LIVER10DBD
LIVER PROFILE - 4 Ag M2, LKM1 (ricombinante P4502D6), LC1 (liver cytosol1), SLA AL AD-LIVER4DBD
LIVER PROFILE - 5 Ag M2PDH, LKM1, LC1, SLA, F-Actina AL AD-LIVERDBD
LIVER PROFILE - 7 Ag LKM1 (ricomb. P450 2D6), LC1, M2-Ric, SLA, F-Actina, gp210, Sp100 AL AD-LIVER7DBD
MALATTIA CELIACA
DotDiver CELIAK IGA - 2 Ag Transglutaminasi, Gliadina deamidata GA 5014
DotDiver CELIAK IGG - 2 Ag Transglutaminasi, Gliadina deamidata GA 5015
MALATTIE GASTROINTESTINALI E INTOLLERANZE ALIMENTARI
ASCA SCREEN - 1 Ag Mannani di S. Cerevisiae AL AD-ASCASDBD
INTOLLER. ALLE PROTEINE DEL LATTE - 2 Ag Beta lattoglobulina, Soia AL AD-BLSODBD
ANTI-FATTORE INTRINSECO - 1 Ag Fattore intrinseco AL AD-IFDBD
DotDiver BiermAK - 2 Ag Fattore Intrinseco, Cellule Parietali GA 5019
TROMBOSI
CARDIOLIPINA + BETA2 GPI - 2 Ag Cardiolipina, Beta 2 glicoproteina AL AD-CBGDBD
DotDiver PHOSPHOLIPID IgG - 9 Ag Cardiolipina, Acido Fosfatidico, Fosfatidiletanolamina, Fosfatidilglicerolo, Fosfatidilinositolo, Fosfatidilserina, Annessina V, Beta2-GP1, Protrombina
GA 50401
DotDiver PHOSPHOLIPID IgM - 9 Ag Cardiolipina, Acido Fosfatidico, Fosfatidiletanolamina, Fosfatidilglicerolo, Fosfatidilinositolo, Fosfatidilserina, Annessina V, Beta2-GP1, Protrombina
GA 50411
QUANTRIX
ANA PROFILE - 19 Ag Nucleosomi, dsDNA, istoni, Sm , RNP (68Kd/A/C), Sm/RNP, SSA 60kD, SSA 52kD, SSB, Scl70, RNA Polimerasi III, Ku, PMScl, Mi-2, Jo-1, CENP A/B, PCNA, DFS70, Ribosomi P
AL AD-ANA19DBDM
DotDiver ANA PROFILE - 25 Ag Nucleosomi, dsDNA, istoni, Sm , RNP (68Kd/A/C), Sm/RNP, SSA, TRIM 21, SSB, Scl70, Ku, PMScl, Mi-2, JO1, PL7, PL12, SRP, Ribosomi, CENP A/B, PCNA, sp100, gp210, M2 (OGDC-E2,BCOADC-E2,PDC-E2) Ric, M2 nativo, F-Actina
GA 5020
CYTOPLASMATIC PROFILE - 8 Ag Jo-1, PL-7, PL-12, EJ, SRP, M2/nPDC, M2 ricombinante e Ribosoma P0 AL AD-CYTO8DBDM
LIVER PROFILE - 10 Ag M2/nPDC, M2/OGDC-E2, M2/BCOADC-E2, M2/PDC-E2, gp210, sp100, LKM1, LC1, SLA, F-Actina AL AD-LIVER10DBDM
NUCLEOLAR - 3 Ag Scl-70, RNA Polimerasi III e PM-Scl 100 AL AD-NUC3DBDM*di prossima introduzione