scientific talks in general and srip in particular
TRANSCRIPT
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Scientific Talks
in general and
SRIP
in particular.
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The Ten Minute Rule
No matter how much time has been allottedImagine that you have only 10 minutes
This will force you to isolate the important information
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Distill your data down to one point
Then state the significance in one sentence
This is the THM
Step 1 :
Isolate the Take Home Message (THM)
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Mike Tyson vs Evander Holyfield
Janet Jackson and Justin Timberlake
Don’t Drown In Your Data
Months later, nobody will remember the details!
For example what was memorable about:
What color were her shoes?
What round?
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Your data is complex
But there is one veryimportant piece of data
Isolate it!
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Step 2 The Introduction
Purpose- Start from nothing and get to the THM
Remember:
Who’s your Daddy? (your audience)
Just the Facts (Don’t show off)
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•Determine the “main question” or hypothesis
•Very Specific
•The answer will have broad implications and be the THM
Remember:
Who’s your Daddy?
Just the Facts
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Introduction
(The question)
Conclusion
Results
(The data and interpretation)
(The THM )
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Q. How much data?
Q. How many slides?
A. = T/2(rule of thumb)
For a 10 minute talk, 5 slidesFor a 30 minute talk, 15 slidesetc
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The main question dictates the data you present
Data orSlide orOverhead
?YES, then keep it
NO, then toss it
Who’s your Daddy?
Does the data address the question?
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Results Section
•Longest section = .7 T•Logical flow•Avoid vague statements•Draw conclusions (different from THM)
Conclusion:
Mutations in abc1 cause abnormal eye development
THM:
Eye development is regulated by ubiquitylation
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Know Your Data
•Practice without it
You must know:
•What the data are•What the data mean•Where the data lead
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Step 3 The Conclusion
•Recap the question
•Recap the most important conclusion(s)
•State the THM
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For Clarity -The Three Tellums
1. Tellum what you’re gonna tellum
2. Then tellum
3. Then tellum what you toldum
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Slides - audiovisual aids
(The important word is “aid”. Remember….this is a TALK)
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Tor signaling
•Tor is a highly conserved PI3 (lipid) kinase that is a protein kinase in vitro, and presumably in vivo.•So-called because it is the “target of rapamycin”•Rapamycin is a drug that was identified from the soil of the island of “Rapa Nui” (also known as Easter Island) in a screen for anti-fungal compounds•Yeast mutants resistant to rapamycin identified three genes TOR1 TOR2 and FRP1•FRP1 encodes a homolog of FK-506 binding protein•It is rapamycin bound to FK-506 binding protein that binds to and inhibits the Tor1 and Tor2 protein kinases
Here’s an example of a really bad slide.(Nobody came to see if you can read)
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YEPD YEPD + Ben
Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2
A
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
B
C WCE IgG IP Sup-IgG IP
Mad3-PrA
Mad2
Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +
1 2 3 4 5 6 7 8 9
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
D
Don’t show figures from papers with no context
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Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Keep it simple.This slide explains the important experiment
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Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Colors are not necessarily good
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Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Background colors are OK but be careful
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Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Avoid Powerpoint backgrounds orThe curtain goes down on your career
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Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Sometimes nice to break things up
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YEPD YEPD + Ben
Wild typeMAD3-PrAMAD3-PrA CDC20-13MYCmad2
A
Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
B
C WCE IgG IP Sup-IgG IP
Mad3-PrA
Mad2
Mad3-PrA - + + - + + - + + Cdc20-13Myc + - + + - + + - +
1 2 3 4 5 6 7 8 9
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
D
VERY BUSY
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Immunodeplete Mad3-PrA (IgG Sepharose)
(IgG IP)
WCE
SUP(IgG IP)
Immunodeplete Cdc20-13Myc (anti-Myc beads)
(Myc IP) SUP(Myc IP)
Isolate the important information (still too busy)and lots of white space
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Pull down Mad3-PrA
(Blot Mad2)
WCE
SUP
Pulldown Cdc20-13Myc
Mad3
PrA
Mad2 Mad2
Cdc20
Myc
(Blot Mad2)
Sequential Immunoprecipitation
Simplify with title, graphic, essential words
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Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Isolate the important data
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Mad3
PrA
Mad2 Mad2
Cdc20
Myc
Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Even better
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Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Mad2
Cdc20
Myc
Mad3
PrA
Mad2
Avoid Powerpoint Nonsense
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Sup-IgGIP IgG IP Myc IP Sup- Myc IP
Mad2
Cdc20-13Myc
Mad3-PrA - + + - + + - + + - +
Cdc20-13Myc + - + + - + + - + + +
1 2 3 4 5 6 7 8 9 10 11
Mad2
Cdc20
Myc
Mad3
PrA
Mad2
or find a new career
Don’t get carried away!
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“Every product of genius is a product of enthusiasm”
Benjamin Disreali
The Delivery
Be yourself and for Pete’s sake RELAX!
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And finally…………..
Dress for Success
Men – Business Casual
Women – Professional not Provocative
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The Ten Minute Rule
No matter what the talk/seminar is:
T/2 slides
Be professional
My Take Home Message: