screening of advanced glycation end-products (age) inhibitory activity of extracts from gymnema...

7/30/2019 Screening of Advanced Glycation End-Products (AGE) Inhibitory Activity of Extracts from Gymnema Sylvestre and Costus Spiralis

1/13

Screeningofadvancedglycation

endproducts

(AGE)

inhibitory

activity

ofextracts fromGymnema sylvestre

andCostus spiralis

Ivette Sierra

Vernica Aponte

Dra.Jannette

Gavilln

Surez


2/13

Maillard reaction

Thisreactionoccurinthehumanbodyandisdividedin

twostages:

Theearlyandreversiblestageistheformationof

Amadori compoundsandSchiffbase.

Thelateandirreversiblestageistheformationof

fluorescentproteinscontainingadvancedglycation end

products(AGE)

Protein+Sugar Schiff Amadori AdvancedGlycation

Base Products EndProducts(AGE)


3/13

Advancedglycation endproducts

(AGE) Areaheterogeneous group of molecules formed from the nonenzymatic

reaction of reducing sugars with freeaminogroups of proteins.

Fluorescentcomplexformedofasugarandaprotein.

Have the ability for covalent crosslink formation between proteins,which

alters their structure and function.

e.g.pentosidine

Ayra,Diaz,1999


4/13

DiabetesandAGEinhibition

AccumulationofAGEisonecauseofdiabetescomplicationssuch

cataracts,impairedwoundhealing,vasculardamageandarterialwall

stiffening.

10.6%ofPuertoRicanpopulationsuffersdiabetes.

Aminoguanidine havebeenreportedasinhibitoroftheMaillard reaction

buttheeffectivedoseisdangeroustothehumanbody.

Plantsand

natural

products

such

quercetin,

aspirin,

vitamin

B

represent

analternativetreatmenttopeoplewithdiabetes.

http://www.salud.gov.pr/Publicaciones/Articulos/Pages/EpidemiologiadelaDiabetes.aspx


5/13

Gymnema sylvestre

TheadministrationofGymnema

sylvestre forthe

treatment

of

diabeteshasbeensupportedby

experimentalresearchfindings.

Stimulatesinsulinreleaseand

regeneratespancreaticbetacells.

Leach,2007


6/13

Costus spiralis

Is widely used intraditional

medicineof Puerto

Rico

to treat

various diseases including

diabetes.

Da.MarianaCruzVzquez,

Cidra:Elt delas hojas deinsulina baja elazcar.

M.Benedetti,1996


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Hypothesis

Gymnena sylvestre andCostus spiralis methanolicextractsinhibitAGE

formation.

Specificaims

TodeterminethepercentofAGEinhibitionofGymnena sylvestre and

Costus spiralis methanolicextracts.

Tocompare

the

percent

of

AGE

inhibition

of

Gymnena sylvestre and

Costus spiralis methanolicextractswithAminoguanidine.


8/13

Methodology

Invitro glycation Invitroglycation under

hyperglycemiaconditions.

Samplesincubatedat60C

SamplesAnalysis Samplesanalyzedbyspectroscopy

EX 350

nm;

EM

450

nm

DataAnalysisInhibition

percent

=Fluorescence

Intensity

(FI)

Control

FI

Test

x100

FIControl


9/13

Results

Inhibitionpercentat30h

68 %

43 %

14 %

0 10 20 30 40 50 60 70 80

%inhibition

G.sylvestre(20mg/mL)C.spiralis(20mg/mL)

Aminoguanidine(10mM;0.62mg/mL)


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Conclusion

ThedecreasingorderofAGEinhibitionwas:

Aminoguanidine >C.spiralis >G.sylvestre Theresultssuggestthattheantidiabetes activityofGymnema

sylvestre isnotrelatedtoAGEinhibition.

Thehypothesiswasproven.


11/13

Futurework

UseotherconcentrationsofCostus spiralis tocalculateIC50

.

StudyinhibitoryactivityofaqueousextractsofGymnema

sylvestre andCostus spiralis.


12/13

References

Ayra M.,DiazO.,1999,Productos delaglucosilacion avanzada ydiabetesmellitus,RevCubana End,10,5764

LeachM.,2007, Gymnema sylvestre forDiabetesMellitus:ASystematicReview,J.Alt.Medicine,9,pp.977983

Matsuura,N.,

Aradate,

T.,

Sasaki,

C.,

Kojima,

H.,

Ohara,

M.,

Hasegawa,

J.,

Ubukata,M.,2002,ScreeningsystemfortheMaillard reactioninhibitorfromnaturalproductsextracts.J.HealthSci.48,520526

Wirasathien L.,Pengsuparp T.,

Suttisri R.,

Ueda

H.,

Moriyasu M.,

Kawanishi K.,

2007,Inhibitorsofaldose reductase andadvancedglycation endproductsformationfromleavesofStelechocarpus cauliflorus,Phytomedicine,14,546550


13/13

Screeningofadvancedglycation

endproducts

(AGE)

inhibitory

activity

ofextracts fromGymnema sylvestre

andCostus spiralis

Ivette Sierra

Vernica Aponte

Dra.Jannette

Gavilln

Surez

screening of advanced glycation end-products (age) inhibitory activity of extracts from gymnema...

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