seed and crop making the difference - in1touch

MAKING THE DIFFERENCESEED AND CROP

SUSTAINABLE AND INNOVATIVE SOLUTIONS

2© SGS SA 2018 All Rights Reserved

SEED AND CROP SERVICES - GLOBAL

SGS is recognized as the world’s leading testing, certification, and verification company. SGS founded in 1879, headquartered in Geneva, traded publicly on Swiss exchange

Global reach of SGS – Seed and Crop Soils

• Laboratory Analysis• Precision Agriculture• Fertility Management

Greenhouse & Field Production• Laboratory Analysis & Food Safety

Forage & Feed• Laboratory Analysis

Seeds• Seed Analysis (CND, ISTA, AOSA)


ACCREDITATION - SEED TESTING

Legislation Canada Seeds Act, Seeds Regulations, Weed Seed Order Variety Registration, Seed Certification, PBR legislation

Seed Lab Regulatory Body Accreditor: CFIA SSTS (Seed Sciences and Technology Section) Auditor: CFIA and CSI (Canadian Seed Institute)

Accreditation - Lab SEED LAAP: Seed Laboratory Accreditation and Audit Protocol Accreditation Steps:

• Application, submission of QM, proficiency testing, audit Minimum facility/ asset requirements, Mandatory Documentation

• Issuance of audit report, correction of non-conformances, accreditation

Accreditation - Staff Inhouse Training & CFIA Preparatory Workshops CFIA Examination


AUDIT - SEED TESTING

Laboratory Audit of Competencies ASLPMP: Accredited Seed Testing Laboratory Proficiency Monitoring

Program. Principled after ISO/IEC Guidelines for 17025.• CSI Audit: 3 year rotation (A,B,C, BMP)• Internal proficiency monitoring: Ongoing• CFIA PT program: 2 panels per year. Rating on Z-Scores

Analyst Audit of Competencies CSAAC (Commercial Seed Analyst Association of Canada)

• Membership status thru continued education points: Workshop, Education• Revoke of CSAAC seal usage


STANDARD METHODOLOGY- GERMINATION

Defines: Sample Setup - Substrata, Temperature, Duration, Replication Analysis Reporting, Rounding Re-Test Rules


STANDARD METHODOLOGY - GERMINATION

Analyzing for: Normal Seedlings (A.) Abnormal Seedlings (C.) Fresh Seeds Dead Seeds (B.) Hard Seeds


DAY 2 DAY 3

Why Fusarium is important!

STANDARD METHODOLOGY- GERMINATION


Analyzing for: Weeds

• Prohibited, Primary, Secondary, Other• 2017 Modernization

Disease bodies Other Crops

Grade Table Benchmarks Reported

• quantification by analyzed portion• % by weight

STANDARD METHODOLOGY - PURITY


Analyzing for: Ustilago nuda NON-VISIBLE embryo infection

Grade Table Maximum 2% to seal as Certified #1 (Seed

Grade Tables) unless treated with an registered fungicide

Reported % Infection

STANDARD METHODOLOGY – TRUE LOOSE SMUT


ADVANCED METHODOLOGY - COOL STRESS TEST

PURPOSE: To gain information regarding the overall performance of seeds placed under low temperature stress.

METHOD: AOSA Seed Vigor Workbook, UofM Vigor Publication (E. Stobie). Standards for test conformity.

MEASUREMENT: Vigorous Seedling, Non-Vigorous Seedling, No growth

INSTRUMENTATION: Petri Dish Chill, followed by warm chamber


ADVANCED METHODOLOGY - CLEARFIELD CONFIRM

PURPOSE: To determine the tolerance of the submitted sample to Imazamox herbicide treatment using a seed based germination assay

METHOD: BASF Method

MEASUREMENT: Pass or Fail

INSTRUMENTATION: Growing media. Imazamox herbicide (120g/L). Warm chamber


ADVANCED METHODOLOGY – FUNGAL SCAN

LATIN NAME COMMON NAME IMPORTANCE

PATHOGENIC FUNGI

Cochliobolussativus

Root rot, seedling blight, late stage root rot, Spot Blotch

Reduce stand density and yield potential

Fusarium graminearum

Seedling blight, fusarium head blight, fusarium damaged kernels, vomitoxin contamination

Fusarium spp. Mycotoxin production, root/crown rot

Pyrenophora(Dreschlera) spp.

Tan spot, net blotch (barley)

Septoria spp. Leaf blotch

WEAKLY PATHOGENICFUNGI

Alternaria spp. Black point, smudge Not expected to cause establishment issues, but do impact kernel physical traits

Cladosporium spp. Mildew

Epicoccum spp. Kernel staining

STORAGE FUNGI

Aspergillus spp. Storage mold Pathogens that identify storage issues and seed quality deterioration

Penicillium spp. Storage mold


CEREAL SEED DISEASESFUSARIUM SPP.

F. AVENACEUM F. CULMORUM

F. POAE F. SPOROTRICHIOIDES

F. GRAMINEARUM

Fusarium spp.

F. graminearum- Seedling blight, fusarium damaged kernels, DON, 3-A DON chemotype increasing. ‘No Detect Policy now changed’. ZON. NIV

F. culmorum – DON, ZON, NIV

F. poae (NIV, HT-2,T2), sporotrichiodes (HT-2, T2)


ADVANCED METHODOLOGY – WHEAT MIDGE REFUGE TESTING

PURPOSE: Additional Certification Requirement. Quantify % refuge seeds in harvested sample from blended seed field.

METHOD: Sub-contracted to SRC.

MEASUREMENT: Based on anticipate pedigree seeking. Select to Certified

REQUIREMENTS: CSGA/Breeder presets % refuge acceptable range. Remedial action if outside of range.


ADVANCED METHODOLOGY – CDC TRIFFID GMOTESTING

WHY: July 2009 - a commercial EU lab detected GM material in Canadian shipment of flax. FP967 received regulatory (1996) and food safety authorization (1996), but not commercialized.

PURPOSE: Detection of FP967 event

METHOD: RT-PCR (extraction, amplification, target DNA florescence)

MEASUREMENT: Detected or Not Detected (2kgs)

INDUSTRY RESPONSE: Re-Constitution in 2014


ADVANCED METHODOLOGY - SEED TREATMENT TESTING

DUST OFF

PURPOSE: Quantify dust removed when standardized air flow passed through treated seed sample

METHOD: Global method

MEASUREMENT: mg/100 g seed

INSTRUMENTATION: Heubach Digital Scale: 5 digits


ADVANCED METHODOLOGY – SEED TREATMENT TESTING

ACTIVE INGREDIENT PURPOSE: Measurement of specific active ingredient on treated

seed METHOD: Analytical differential partitioning and quantification MEASUREMENT: g / 100 kg treated seed INSTRUMENTATION:

Sonicator High Performance Liquid Chromatography


ADVANCED METHODOLOGY EXPIDITING INTERNATIONAL SHIPMENTS

ISTA Orange Certificate (OIC) Limited lot size, ISTA sampler

ISTA Blue (BIC)

EU Grading Reports

PURPOSE: Facilitate international shipments based on importing country seed and phytosanitary requirements

METHOD: ISTA and EU Seed Schemes

TESTS Germination % Pure Seed Other Seed Determination Tetrazolium, Moisture, Kernal Size


ADVANCED METHODOLOGY – GRAINS ANALYTICS

Accredited methods: AACC, AOAC

Assist breeders with new variety testing - from early generation to registration trials

Perform New Harvest Quality Survey for milling and grain companies

Quality assessment of wheat shipments - by vessel or hold Report issued prior to shipment arriving at destination – helps with

planning accordingly (wheat storage, flour ingredients)


FUTURE SEED DIAGNOSTICS FOR COMMERCIALIZATION

XRAY Difficult to germinate Internal Structure review

AI/MACHINE LEARNING Physical Purity – Remote, Process improvement

REGULATORTY MODERNIZATION Global Alignment of test regulations Transition to Private Sector Replacement of in-field inspections with molecular genetics


CONSIDERATIONS – UNDERSTANDING SEED QUALITY

In-Season Growing Disease Staging and uniformity

Harvest Pre-harvest herbicide Frost/heat Rain/snow prior Combine speed and fan settings

Storage Handling Seed moisture Seed temperature

Drying Temperature Duration


• Germination• Purity• Vigor• Kernel Size• Seed Disease• Tetrazolium• ISTA Analysis,

Sampling & Training• APHIS & SICA• Herbicide Bioassays• Varietal Identification• Soil & Tissue Pathology• Dust off & Active Ingr.• Soil Seed Analysis• REGAL

SEED AND CROP SERVICES – CANADA

SHERWOOD PARK GRANDE PRAIRIE

• Germination• Purity• Vigor• Kernel size• Tetrazolium• APHIS• Grade• AOSA focus

• Grasses, Forages, Herbs, Vegetables, Native Species

GUELPH

• Crop Production analysis (Soil, Fertilizer, Manure, Plant Tissue, Water)

• Livestock Nutrition -Forage and feed (NIR/Wet Chemistry)

• Greenhouse (Media, Water, Tissue)

• Nutrient & Disease Scans (Soil, Tissue, Irrigation)

• Companion Animal Food Testing


CONTACT US

Holly Gelech [email protected] 780.660.9657 www.biovision.ca Twitter: Seed_Testing Instagam: Sgs_SeedandCrop_Canada

mailto:[email protected]

http://www.biovision.ca/

seed and crop making the difference - in1touch

Documents