selected references ashrae. ashrae handbook. atlanta: 2005
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Input-output Correlation Sensitivity Analysis for Environmental Standards for Bacillus Anthracis Spores. Tao Hong 1 , Patrick Gurian 1 , Nicholas Dudley Ward 2 1 Department of Civil, Architectural and Environmental Engineering, Drexel University 2 Pattle Delamore Partners, New Zealand. - PowerPoint PPT PresentationTRANSCRIPT
Selected referencesASHRAE. Ashrae handbook. Atlanta: 2005.Bartrand, T. A., M. H. Weir, et al. Dose-response models for inhalation of bacillus anthracis spores: Interspecies comparisons. Risk Analysis, 2008; 28 4: 1115-1124.Brachman, P.S., 1966. “Industrial Inhalation Anthrax,” Bacteriological Reviews, 30(3):646-656. Druett, H.A., 1953. “Studies on Respiratory Infection. I. The Influence of Particle Size on Respiratory Infection with Anthrax Spores,” Journal of Hygiene, 51:359-371. EPA. Exposure factors handbook. Washington, DC : Exposure Assessment Group, Office of Health and Environmental Assessment, U.S. Environmental Protection Agency, 1997.Hass, C.N. 2002. “On the Risk of Mortality to Primates Exposed to Anthrax Spores,” Risk Analysis, 2(2):1218-21. Tao Hong (2009). Estimating Risk of Exposure to Bacillus Anthracis based on Environmental Concentration. Civil, Architectural and Environmental Engineering. Philadelphia, Drexel. M.S. thesis.Kowalski, W. J. Immune building system technology. New York: McGraw-Hill, 2003.Lai, A. C. K. and W. W. Nazaroff. Modeling indoor particle deposition from turbulent flow onto smooth surfaces. Journal of Aerosol Science, 2000; 31 4: 463-476.Landahl, H. On the removal of air-borne droplets by the human respiratory tract: Ii. The nasal passages. Bulletin of Mathematical Biology, 1950; 12 2: 161-169.Mitchell-Blackwood, J. and P. L. Gurian (2008a). An evaluation of the risk threshold for prophylaxis and treatment after an anthrax release.Mitchell-Blackwood, J., P. L. Gurian, et al. (2008b). A hierarchical model for probabilistic dose-response assessment of bacillus anthracis.NRC. Reopening public facilities after a biological attack: A decision-making framework. 2005.Teunis, P. F. M. and A. H. Havelaar. Risk assessment for protozoan parasites. International Biodeterioration & Biodegradation, 2002; 50 3-4: 185-193.Thatcher, T. L. and D. W. Layton. Deposition, resuspension, and penetration of particles within a residence. Atmospheric Environment, 1995; 29 13: 1487-1497.Sextro, R. G., D. M. Lorenzetti, et al. Modeling the spread of anthrax in buildings. 2002; Schneider, T., J. Kildeso, et al. A two compartment model for determining the contribution of sources, surface deposition and resuspension to air and surface dust concentration levels in occupied rooms. Building and Environment, 1999; 34 5: 583-595.
Conclusion1. For prospective and retrospective scenarios, the risk of mortality can be linked with the concentration of Bacillus anthracis on surfaces and HVAC filters;
2. Accurately measuring these levels presents an analytical challenge. Detecting well-aerosolized B. anthracis (diameter of 1 µM) will be particularly problematic;
3.Uncertainties in dose response contribute the most to overall uncertainty in standards; uncertainties in deposition velocities are significant sources of uncertainty for the larger particle sizes, while HVAC recirculation rate contributes substantial uncertainty to the 1 µM particles;
4.This analysis does not account for decay over time, recovery, or discounting of future risk.
Model Introduction
A. Fate and transport model
Fig. 2. Schematic of model
The model was built based on a simple occupied office suite with a heating ventilation and air conditioning (HVAC) system as in Fig. 2. The office was divided into 7 internal compartments: air, tracked floor, untracked floor, walls, ceiling, HVAC, and in the nasal passages of an occupant of the office.
The following system of linear first order ordinary differential equations was obtained based on the mass balance relationships among different compartments in the office suite:
•Mce(t) is the Bacillus anthacis on the ceiling at time t.
•Mn(t) is the Bacillus anthacis in people’s nasal passages at time t.
•Mair, Mtf, Mutf, Mw, Mf, Mec, Mce and Mn are all given in units of spores (number of organisms).•Q is the discharge from the air compartment (computed from ACH assumptions). Units are [m3] •λtf, λutf, λw and λce are the deposition rates onto the tracked surface, the untracked surface and the floors, walls and ceiling respectively. Units are [T-1]•µ2 is the resuspension rate from the untracked surface into the air compartment. Units are [T-1]•p is the fraction of air recirculated into the building by the HVAC system. •e is the efficiency of the filter at removing particles.•en is the efficiency of the nasal passages at removing particles.•I is the rate of the occupant. Units are [m3/hour].•V is the volume of model the room Units are [m3].
Background
Bacillus anthracis is an aerobic, Gram-positive, non-motile, spore-forming bacterial species which is the causative agent of anthrax, a potentially fatal bacterial infection. Envelops containing Bacillus anthracis spores were mailed to news media companies and government officials separately in 2001 (Fig. 1), which caused the death of 5 people and cost hundreds of millions of dollars to clean the contaminated buildings.
Fig.1. Letters used in 2001 anthrax attacks (FBI)
Since human health risk from biological agents is associated with aerosol exposures, it is suggested that concentrations found on surfaces may be used to infer future or past aerosol exposures. When these concentrations indicate that aerosol exposures exceed specified values, they would trigger response actions, such as remediation (to avoid future risks) or prophylactic antibiotics (to mitigate risks of past exposure).
B. Dose-response model
II. Prospective scenario
In the prospective scenario, the risk is related to the concentration of Bacillus anthacis on the tracked floor under the assumption that spores from other surfaces are not reaerosolized. The inhaled dose is determined by integrating equation (6) with Cair expressed as mass of Bacillus anthracis spores in the aerosol compartment divided by volume of the room, when times goes infinity:
(7)
Where Γ is termed future risk coefficient.
ResultsNumerical values of model input parameters are provide in Tables 2. Fig. 3 and Fig. 4 shows the relationship between accumulative risk of mortality and time for different particle sizes for retrospective scenario and prospective scenario, respectively. For detail please refer to Hong (2009).
Table 2. Model inputs
Monte Carlo simulation
Monte Carlo simulation is fulfilled based on the ranges of inputs from Table 2, and uniform distribution is assumed between boundaries. Simulation results are presented in Fig. 5, Fig 6 and Table 3.
Tao Hong1, Patrick Gurian1, Nicholas Dudley Ward2
1Department of Civil, Architectural and Environmental Engineering, Drexel University 2Pattle Delamore Partners, New Zealand
Previous studies indicate that exponential function (Eq. 1) provides a favorable fit for Bacillus anthracis spores with particle size smaller than 5μM, and beta-Poisson function (Eq. 2) fits well for spores with diameters larger than 5 µM (Bartrand, Weir et al. 2008; Teunis and Havelaar 2002; Haas 2002):
risk =1- exp(-r dose) (1)
(2)
where risk is the probability of infection, dose is the average number of inhaled spores, r is the probability that a single organism will survive to initiate infection, and α, β are parameters of beta distribution. When the product of r and dose is relatively small, a first-order Taylor series can be used to approximate equation (3) and (4) as:
risk =r dose (3)
(4)
N50 is median infection dose. Since the initial conditions for retrospective and prospective risk are different, we consider these two different scenarios separately in the following.
C. Release scenarios
Two scenarios on individuals exposure to Bacillus anthracis are taken into account. One scenario is termed retrospective risk and the other prospective risk. For the retrospective scenario, an individual was assumed to be exposed to Bacillus anthracis spores which were released in the air of a room instantaneously, while for the prospective scenario, it was assumed that Bacillus anthracis has been deposited on a tracked surface, which corresponds to a post-event scenario following settling. Mathematically this corresponds to changing the initial conditions to a release on the tracked floor and using the same model that had been as previously described for the retrospective scenario. Retrospective risk estimates would inform measures for mitigating prior exposures, such as administering prophylactic antibiotics, while prospective risk estimates would inform decisions for mitigating future exposures, such as environmental remediation and re-occupancy standards for contaminated buildings.
D. Linking risk with surface concentration
I. Retrospective scenarioConcentrations in several of the environmental compartments are linear functions of the integral of past air concentrations. As the dose is also a function of the integrated air concentration, it is possible to obtain relationships between environmental concentrations and retrospective human health risk in which time is not an explicit variable. The concentration of Bacillus anthacis on the untracked floor is the integrated number of spores deposited over time (ta-tb), divided by the area of the untracked floor, Auf: (5)
Assuming that the occupants of the room have experienced the exposure from the beginning (a health protective assumption as discussed above), which can be expressed mathematically as t1= ta= 0 and t2= tb, one can combine equation (5) and equation (6) to link the dose with the concentration on untracked horizontal surfaces: (6)
(7)
The deposition rate onto the untracked floor for Bacillus anthacis (λuf) is a function of deposition area, volume of the room, and corresponding deposition velocity (νuf with the unit m/s) (Thatcher and Layton 1995). Thus equation (7) can be rewritten as: (8)
At low doses, equation (8) can be combined with equation (1), the Taylor series approximation of an exponential dose-response function, to link the probability of mortality with the concentration of Bacillus anthacis on the untracked floor (Eq. 9). Table 1 includes surface concentrations-risk equations for other surfaces.
(9)
AcknowledgeThis project was funded through the CAMRA Center of Excellence by a grant from the Department of Homeland Security, Science and Technology Directorate, Office of University Programs and the Environmental Protection Agency. The authors also would like to acknowledge Professor Agami Reddy’s valuable input on HVAC system parameters.
Input-output Correlation Sensitivity Analysis for Environmental Standards for Bacillus Anthracis Spores
It is generally accepted that the U.S. remains vulnerable to the risk of suffering another bioterrorist attack. However, the US lacks guidelines for a quick response to such attacks which will allow responders to estimate who were exposed during the attack and what level of risk these exposed individuals face; also the U.S. does not have standards for what is the safe concentration of Bacillus anthracis for reoccupation of a building after decontamination.
n
ce
ec
f
w
utf
tf
air
n
ce
w
utf
tf
ncewutftf
n
ce
ec
f
w
utf
tf
air
M
M
M
M
M
M
M
M
V
eInh
V
Qp
V
Qep
μ
μV
eI
V
Qpe
M
M
M
M
M
M
M
M
dt
dM
0000000
0000000
0000000)1(
0000000
0000000
0000000
000000
000000)
(]1)1[(
2
2
•Mair(t) is the Bacillus anthacis in the air compartment at time t.•Mtf(t) is the Bacillus anthacis on the tracked surface of the floor at time t.•Mutf(t) is the Bacillus anthacis on the untracked surface of the floor at time t.•Mw(t) is the Bacillus anthacis on the walls at time t.
•Mf(t) is the Bacillus anthacis on the filter at time t.
•Mec(t) is the Bacillus anthacis in the external compartment at time t.
doseN
doserisk)
12(
/1
50
)1(1
doserisk
b
a
t
tair
uf
uf
uf
ufuf dttC
A
V
A
MC )(
2
1
)( t
t air dttCIdose
I
dosedttC
V
CA bt
airuf
ufuf0 )(
uf
ufCIdose
uf
ufCIrrisk
Equation used to compute clean up standard Surface
Walls
HVAC filter
Nasal passages
Table 1 Surface concentration-risk equation
Qpe
CAIrrisk ff
n
nn
e
CArrisk
w
wCIrrisk
V
IACr
DD
DD
V
IACrrisk tftftftf
)(
)( 0,
1,22,12,21,121
1,12,1120,
Symbol Meaning Units Diameter
Value scale
Input valueLower
boundSource
Upper
boundSource
Vuf, Vtf
Deposition velocity on untracked and
tracked floorm/s
1μM 3.5×10-5(Lai and Nazaroff
2000)8.0×10-4
(NRC 2005)
(Riley, McKone et al. 2002)
6.9×10-5
3μM 2.0×10-4
(NRC 2005)6.0×10-3 4.2×10-4
5μM 3.0×10-4 1.4×10-2 1.4×10-3
10μM 7.0×10-4 2.7×10-2 5.6×10-3
Vw
Deposition velocity on walls
m/s
1μM 3.5×10-8
(Lai and Nazaroff 2000)
9.0×10-5
(Schneider, Kildeso et al. 1999)
3.9×10-5
3μM 1.5×10-8 2.1×10-4 1.6×10-4
5μM 1.0×10-8 4.0×10-4 3.1×10-4
10μM 7.0×10-9 6.0×10-4 3.5×10-4
Vce
Deposition velocity on ceiling
m/s 1μM (NRC 2005) 6.2×10-7
en
Nasal passages particle remove
efficiency
1μM 0.02
(Landahl 1950)
0.25
(Roger O. McClellan and
Henderson 1989)
0.14
3μM 0.22 0.68 0.45
5μM 0.42 0.81 0.62
10μM 0.62 0.91 0.77
R (α/β)
Probability of a single Bacillus anthracis spore
initiating infection
1-5 μM9.1×10-7
(95% confidence interval)
(Mitchell-Blackwood, Gurian et al.
2008b)
7.0×10-5
(95% confidence interval)
(Mitchell-Blackwood, Gurian et al.
2008b)
7.2×10-6
10 μM1.0×10-7
(95% confidence interval)
Extrapolated from
(Mitchell-Blackwood, Gurian et al.
2008b)
8.1×10-6
(95% confidence interval)
Extrapolated from
(Mitchell-Blackwood, Gurian et al.
2008b)
8.2×10-7
riskAcceptable risk
level1.0×10-5
(Mitchell-Blackwood and Gurian 2008a)
1.0×10-3(Travis, Richter
et al. 1987)1.0×10-4
I Breathing rate m3/hr 0.8 (Kowalski 2003) 2.0 (Kowalski 2003) 1.02
pRecirculation
fraction0 (ASHRAE 2005) 1 (ASHRAE 2005) 0.8
Symbol Meaning units Value Source
VRoom
dimensionsm3 5.6×5.6×2.5
Assumed a typical office (EPA 1997;
Sextro, Lorenzetti et
al. 2002)
Atf
Area-tracked floor
m2 5.6×5.6×0.75
Autf
Area-untracked
floorm2 5.6×5.6×0.25
Ace Area- ceiling m2 5.6×5.6
Aw Area- wall m2 5.6×2.5×4
Af Filter area m23.82×10-2
(2.81×10-2-5.62×10-2)
Q/A = 137m/min
(91-183 m/min)
An
Area of nasal passages
m2 0.8(Landahl
1950)
ACHAir changes
per hour4
(ASHRAE 2005)
Q Discharge m3/s 0.087
Q = V×ACH/360
0 (in seconds)
fProportion
tracked0.75
(ASHRAE 2005)
µ2
Resuspension rate
s-1
D=1μM 3.3×10-8(Thatcher
and Layton 1995; Sextro, Lorenzetti et
al. 2002)
D=3μM 5.3×10-7
D=5μM 2.2×10-7
D=10μM 1.1×10-6
eFilter
efficiency
D=1μM 0.098(Sextro,
Lorenzetti et al. 2002)
D=3μM 0.49
D=5μM 0.74
D=10μM 0.88
Fig. 3. Accumulative risk of mortality and time for different particle sizes (retrospective scenario, 1000
spores released)
Fig. 4. Accumulative risk of mortality and time for different particle sizes (Prospective scenario, 1000
spores released)
Retrospective scenario
Risk from inhaling 1µm
spores
Risk from inhaling 3µm
spores
Risk from inhaling 5µm
spores
Risk from inhaling 10µm
sporesDeposition velocity to
tracked floor-0.18 -0.46 -0.55 -0.59
Deposition velocity to wall
-0.04 -0.02 -0.03 -0.05
Human nasal passage particle removal
efficiency0.01 -0.01 0 -0.01
Human breathing rate 0.27 0.29 0.26 0.23
Dose-response coefficient
0.62 0.70 0.61 0.52
Resuspension rate -0.01 0.01 0.02 0.03
HVAC recirculation rate
0.47 0.11 0.03 0.02
Air change rate -0.14 -0.13 -0.09 -0.06
Retrospective scenario
Risk from inhaling 1µm
spores
Risk from inhaling 3µm
spores
Risk from inhaling 5µm
spores
Risk from inhaling 10µm
sporesDeposition velocity to
tracked floor-0.18 -0.46 -0.55 -0.59
Deposition velocity to wall
-0.04 -0.02 -0.03 -0.05
Human nasal passage particle removal
efficiency0.01 -0.01 0 -0.01
Human breathing rate 0.27 0.29 0.26 0.23
Dose-response coefficient
0.62 0.70 0.61 0.52
Resuspension rate -0.01 0.01 0.02 0.03
HVAC recirculation rate
0.47 0.11 0.03 0.02
Air change rate -0.14 -0.13 -0.09 -0.06
Table 3 Correlation between human risk and other input parameters
Fig. 5. Monte Carlo results for retrospective scenario
Fig. 6. Monte Carlo results for Prospective scenario