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Self-Powered Biosensor for Ascorbic Acid with a Prussian Blue Electrochromic Display

Adrianna ZloczewskaAnna CelebanskaKatarzyna SzotDorota TomaszewskaMarcin OpalloMartin Jönsson-Niedziółka

Institute of Physical ChemistryPolish Academy of SciencesWarsaw, Poland

Martin Jönsson-Niedziólka, martinj@ichf.edu.pl Twitter: @ekorrhjulet

Mikrosympozjum sprawozdawcze, IPS PAS.

Institute of Physical Chemistry PAS, Warsaw Poland

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Outline

- Self-powered sensors- CNP electrodes- Ascorbic acid sensing- Biocathode- Ascorbic acid biofuel cell- Self-powered sensor

Photo: Gabriel Hess

Institute of Physical Chemistry PAS, Warsaw Poland

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Photo: Caleb Charland, http://calebcharland.com/

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Self-powered sensor

Fuel cell where output is determined by concentration of analyteE. Katz, A. F. Bückmann, and I. Willner, J. Am. Chem. Soc. 123, 10752 (2001).

Institute of Physical Chemistry PAS, Warsaw Poland

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Truly self-powered sensors

Foto: Jessica Bender

T. Hanashi, T. Yamazaki, W. Tsugawa, S. Ferri, D. Nakayama, M. Tomiyama, K. Ikebukuro, and K. Sode, Biosensors & Bioelectronics 24, 1837 (2009).

BioCapacitor

0.1 mM glucose

1.1 mM glucose

Institute of Physical Chemistry PAS, Warsaw Poland

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Modification of ITO electrodes with carbon nanoparticles

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CNP- solutiona

q

CNP+ solutiona

q

+ carbon nanoparticle modified with positively charged ammonium functionalities

SO

O

N H

NH3+

SO3 carbon nanoparticle modified with phenylsulfonate

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+ +...............

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J.D. Watkins et al. Phys.Chem.Chem.Phys., 12 4872 (2010). K. Szot, et al., Electrochem. Commun. 12, 737 (2010).

1 layer

3 layers

Institute of Physical Chemistry PAS, Warsaw Poland

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Ascorbic acid oxidation

CNP+-CNP- electrode

ITO

M. Kundys et al., Anal. Methods 6 (2014) 7532.

Institute of Physical Chemistry PAS, Warsaw Poland

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Ascorbic acid sensing

Cyclic voltammetry on CNP film electrode in phosphate buffer, pH = 4.8 (dashed line) and in AA solutions of different concentrations: 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 4.0 and 6.0 mM; v = 10 mVs-1

A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.

CNP+-CNP- electrode

Institute of Physical Chemistry PAS, Warsaw Poland

9A. Zloczewska, M Jönsson-Niedziolka, J. Power Sources 228, 104-111 (2013).

RE

CE

WE

Cu tape

silicone spacer

UV-Vis cuvettes with holes

electrolyte

H2O

H+

O2 gas-side

electrolyte-

side

electrolyte

ē

RE – reference electrodeCE - counter electrode

SWCNT – single-walled CNTs

Bilirubin oxidase

Functionalised SWCNT

Silicate matrix

Biocathode

Institute of Physical Chemistry PAS, Warsaw Poland

10A. Zloczewska, M Jönsson-Niedziolka, J. Power Sources 228, 104-111 (2013).

H2O

H+

O2 gas-side

electrolyte-

side

electrolyte

ē

RE – reference electrodeCE - counter electrode

SWCNT – single-walled CNTs

Bilirubin oxidase

Functionalised SWCNT

Silicate matrix

Biocathode

Gas-side filled with: argon a) & b), air c) and dioxygen d). a) the electrolyte was also deaerated.

a)

b)

c)

d)

Institute of Physical Chemistry PAS, Warsaw Poland

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Ascorbic acid BFC

AA-anode and the biocathode working in 1 mM AA,

A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.

Institute of Physical Chemistry PAS, Warsaw Poland

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Ascorbic acid BFC

AA concentrations: 1, 2, 4 and 6 mM.

CNP+-CNP- anode - Air-breathing CNT cathode

A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.

Institute of Physical Chemistry PAS, Warsaw Poland

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Ascorbic acid BFC

CNP+-CNP- anode - Air-breathing CNT cathode

AA-BFC working as a Willner-Katz type self-powered sensor.

CNP anode | CNT air-breathing cathodeCurrent measured at constant E = 0.3V.

A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.

Institute of Physical Chemistry PAS, Warsaw Poland

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Electrochromic display

Prussian Blue display as sensor output. Display prepared by electro-deposition of PB on ITO.

J. J. García-Jareño, D. Benito, J. Navarro-Laboulais, F. Vicente, and J. Garcia-Jareno, J. Chem. Educ. 75, 881 (1998).

Institute of Physical Chemistry PAS, Warsaw Poland

https://crystallography365.wordpress.com/2014/02/02/are-you-feeling-blue-the-structure-of-prussian-blue/

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Truly self-powered sensor

H. Liu and R. M. Crooks, Analytical Chemistry 84, 2528 (2012).

A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.

Institute of Physical Chemistry PAS, Warsaw Poland

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Higher accuracy using image analysis

J. L. Delaney, C. F. Hogan, J. Tian, and W. Shen, Analytical Chemistry 83, 1300 (2011).

Automated analysis of colour of photos taken each 5 s.

Transparency values fitted to find colour after 60 s

A. Zloczewska et al., Biosens. Bioelectron. 54 (2014) 455.

Institute of Physical Chemistry PAS, Warsaw Poland

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Conclusions

We have used carbon nanoparticle electrodes for AA oxidation a low potential.

We can connect these anode to a biocathode to made an AA biofuel cell

The AA BFC can be used a self-powered sensor

It can also be connected to a PB display to make a truly self-powered sensor.

Institute of Physical Chemistry PAS, Warsaw Poland

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Acknowledgements

This work was partially financed by:

The European Union 7.FP under the grant REGPOT-CT-2011- 285949-NOBLESSE

The Polish Ministry of Science and Higher Education under the contract IP2011 020771.

The work of AZ was realized within the International PhD Projects Programme of the Foundation for Polish Science, co-financed from the European Regional Development Fund within the Innovative Economy Operational Programme "Grants for innovation"

Thank you for your attention

NanOtechnology, Biomaterials and aLternative Energy Source for ERA integration

FP7–REGPOT–CT–2011–285949-NOBLESSE

Institute of Physical Chemistry PAS, Warsaw Poland