sequencing genomes€¦ · 1 library prep (~ 6 hrs) 2 automated cluster generation (~ 5 hrs)...
TRANSCRIPT
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CSPro, GenomeStudio, Genetic Energy, and HiSeq are registered trademarks or trademarks of Illumina, Inc. All other brands and names contained herein are the property of their respective owners.
Sequencing
Genomes
Abizar Lakdawalla, PhD
Eur Segment Manager,
Sequencing
Welcome to the
brave new world!
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Clinical sequencing
Three key dimensions :
Genome Breadth: The
fraction of the genome
that is interrogated
Subjects: The number of
participants used in a
study
Clinical Data: The
amount of clinical data
associated with the
individuals
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
The Simplest Sequencing Process
Fragment DNA
Repair ends / Add A overhang
Ligate adapters
Select ligated DNA
Library prep (~ 6 hrs)1
Automated Cluster Generation (~ 5 hrs)2 Hybridize to flow cell
Extend hybridized oligos
Perform bridge amplification
1-8 samples
Sequencing (~ 1-8 days)3 Perform sequencing on forward strand
Re-generate reverse strand
Perform sequencing on reverse strand1-16 samples
1000’s M
DNA!
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Preparing libraries
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Purifying Libraries
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
PCR free modifications
Short Y adapter is replaced with a longer adapter
Adapter primer dimers after ligation are removed by SPRI or
Sephadex beads
Library containing DNA fragments (ligated, partially ligated and non-
ligated) is introduced into flow cells
Bridge amplification is performed on the library
– Non-ligated DNA products do not bind to the flow cell
– Partially ligated products bind but do not amplify
– Ligated products bind and bridge amplify
Cluster size is dependent on sequence content
Algorithms detect all clusters with equal efficiency
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Flow Cell
Simplified
workflow
Clusters in a
contained
environment
(no need for
clean rooms)
Sequencing
performed in
the flow cell
on the
clusters
8 channels
Surface of flow cell
coated with a lawn
of oligo pairs
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Cluster generation
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
PCR free workflow
SPRI
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Reverse transcription in flow cell
1. RNA
2. Fragment
3. Repair
4. Ligate RNA adapters
5. Remove free adapters
and adapter-adapter
dimers
6. Introduce into flow cells
7. Reverse transcribe
8. Bridge amplify
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
The Simplest Sequencing Process
Fragment DNA
Repair ends / Add A overhang
Ligate adapters
Select ligated DNA
Library prep (~ 6 hrs)1
Automated Cluster Generation (~ 5 hrs)2 Hybridize to flow cell
Extend hybridized oligos
Perform bridge amplification
1-8 samples
Sequencing (~ 1-8 days)3 Perform sequencing on forward strand
Re-generate reverse strand
Perform sequencing on reverse strand1-16 samples
1000’s M
DNA!
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Sequencing Forward Strand
Add 4 Fl-
NTP’s +
Polymerase
Incorporated
Fl-NTP is
imaged
Terminator and
fluorescent dye
are cleaved from
the Fl-NTP
X 25 -- 150
Hybridize
sequencing
primer
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Sequencing with Paired Ends
This is really the best way to do sequencing
This is really the best way to do sequencing
This is really the best way to do sequencing
This is really the best way to do sequencing
This is really the best way to do sequencing
This is really the best way to do sequencing
This is really the best way to do sequencing(------26 characters-------)
Reference
Single-reads
…
…
…
…
Paired-reads
Assembly becomes easier!!
Paired end reads
Normal paired ends
Stretched paired end
= deletion
Compressed paired ends
= insertion
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Paired End Sequencing
Sequenced strand
is stripped off
3’-ends of
template strands
and lawn primers
are unblocked
New strand
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Paired End Sequencing
Double
stranded
DNA
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Paired End Sequencing
Bridges are
linearized and
the original
forward template
is cleaved offOriginal
forward
strand
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Paired End Sequencing
Free 3’ ends of
the reverse
template and lawn
primers are
blocked to prevent
unwanted DNA
priming
Reverse
strand
template
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Sequencing Reverse Strand
Add 4 Fl-
NTP’s +
Polymerase
Incorporated
Fl-NTP is
imaged
Terminator and
fluorescent dye
are cleaved from
the Fl-NTP
X 25 -- 150
Hybridize
sequencing
primer
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Genome Analyzer imaging
Obj.
lens
Camera.
.
.
.
.
.
Tile3-4.5 TB/run
640,000 images x 7 MB/image
75-100 x 2 bases
4 images/base
8 channels/flow cell
2 columns/channel
55 tiles/column
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Data Analysis
images intensities base calls
4.8TB 250GB 60GB
Detecting clusters
Measuring the color
for each cluster
… for every cycle
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Data AnalysisA simple, familiar workflow
HiSeq CONTROL
SOFTWARE
Base calls
CASAVA
Alignments,
variations, builds
VISUALIZATION
GenomeStudio, or
favorite browser
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Reads
Aligned
to reference
Read depth
Alignment to reference
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
CASAVA – software to determine variation
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Genome Sequencing Methods
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Sequencing genomes on HiSeq 2000
Target size Sequencing
depth
No. of
samples/ FC
Cost/sample
(Seq only,
2x100)
Cost/sample
(Cluster +
Seq, 2x100)
Aneuploidy 3 Gb 0.3 x ~ 100 € 50 € 85
CNV 3 Gb 1-3 x ~ 10-30 € 170-500 € 280-850
GWAS 3 Mb 30(-50) x ~ 1000 € 5 € 9
Exome 30 Mb 30(-50) x ~ 100 € 50 € 85
SNV
discovery
3 Gb 6 x ~ 5
€ 1000 € 850
SNV/SNP
validation
3 Gb 30x ~ 1
€ 5000 € 8500
Excludes cost of sample prep.
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Sequencing Hu genomes at 0.1-0.3x (€ 100/sample)
1. 75 bp reads at 0.1 x
human genome
coverage
2. Reads map at approx. 1
read every 1 kb
3. Add reads for each
chromosome
4. Divide total reads with
chromosome length
5. Determine chromosome
count
Norm
aliz
ed c
hro
mosom
e c
ount
Chromosome
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Y
One X and one Y chromosome
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Sequencing Hu genomes at 0.1-0.3x (€ 100/sample)
1. 75 bp reads at 0.1 x
human genome
coverage
2. Reads map at approx. 1
read every 1 kb
3. Add reads for each
chromosome
4. Divide total reads with
chromosome length
5. Determine chromosome
countNorm
aliz
ed c
hro
mo
som
e c
ount
Chromosome
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Y
Trisomy 21
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
–“Sequencing is the clear way to
do non-invasive prenatal testing.
… existing noninvasive Down
syndrome tests are not very
informative and provide variable
results depending on the ethnicity
of those taking the test.”
DNA
Sequence
Prenatal aneuploidy by low depth sequencing
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Sequencing whole genomes with paired-ends
Short-insert or long-insert
paired end reads provide
more information on
structural variation
GATCGGTTGCGATTCGG ATCGGTGGGACTGGG
Read spanning a translocation
Normal paired ends
Stretched paired end
= deletion
Compressed paired ends
= insertion
Paired end reads
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Sequencing Hu genomes at 1-3x (€ 850/sample)
1. 75 bp reads at 1 x human
genome coverage
2. Reads map at approx. 1
read every 100 bp
3. Average reads per 1 kb
region
4. Ratio of avg reads for
Sample 1 and 2
5. Plot average read ratio
across chromosomes
6. Determine copy number
variations
Reads/k
b
Chromosome 1
Ratio o
f
reads/k
b
Chromosome 22
CNV
Sample 1
Sample 2
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
ERBB2
BCAS3
CNVs in cancer cell lines with 1x sequencing depth
MCF7
ZR-75-1
T47D
BT474
MDA-MB-231
MDA-MB-468
BT20
MCF10A
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CNV by medium depth sequencing
Array
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
CNV and fusion gene
• 5’ of CACNA2D4 is
amplified
• Paired-end reads show
break in exon 36 of
CACNA2D4 fusing into
intron 3 of WDR43
• Resulting in a fusion
transcript with a shortened
exon 36 from CACNA2D4.
Campbell 2008
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
CNV and inversion
An inverted duplication in
chromosome 17 by localized
increase in copy number.
Two paired-end reads
spanned both inverted
breakpoints.
Campbell 2008
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DNA digestion, fractionation, and
size selection
SNPs by pooled genome sequencing
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SNP Frequencies from Reduced Representation
Libraries
A
T
A
A
A
A
T
A
A
A
A
A
T
A
A
A
A
A
A
A
A
A
Each colour
represents a
read from a
different
genome. Base
frequencies will
indicate SNPs.
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Targeted sequencing by Solid Phase Capture of all Exons
Fragment DNA
Sonication or nebulization
Repair ends
Blunt, phosphorylate.
Ligate linkers (for PCR)
Blunt, phosphorylate, add A-overhang
Target sequence
135 bp covering all exon
Probe design
(forward. strand)
60 bp oligos
with 20 bp overlaps
Probe synthesis
385k oligo
(7 Nimblegen arrays)
Targeted captureHybridize to Nimblegen arrays, wash,
elute, lyophilized and amplified by PCR
Repair & add A-overhang AA
Ligate
Illumina adapters
Grow clusters and sequence
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Sequencing a fraction of the genome
SureSelect™
Target Enrichment System
Sequence only
• Exome
• Cardiac genes
• Diabetes genes
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Clinical sequencing / Seq process / Seq methods / Costs / Seq at low depth / Medm depth / Targeted / Whole genomes / Metagenomes
Exome capture in diagnosis
Unanticipated genetic diagnosis
– congenital chloride diarrhea with a suspected diagnosis of Bartter
syndrome, a renal salt-wasting disease.
– Homozygous in SLC26A3 (known congenital chloride diarrhea
locus).
– 5 additional patients suspected to have Bartter syndrome had
mutations in SLC26A3.
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Targeted sequencing
Familial breast cancer– TP53, BRCA1, and BRCA2 mutations in established tumour cell lines and DNA from patients with
germline mutations. All of the known pathogenic mutations were identified ... clonal sequencing
outperforms current diagnostic methods.
Resistant tumors– Mutations in MEK1, novel mechanisms of resistance, important clinical implications
Cancer-related exome subset
Joubert syndrome 2– Neurological, psychomotor retardation. Mutation in the TMEM216 gene. Hetero- non-symptomatic.
Hereditary poikiloderma– Homozygous A>C mismatch in intron 4 of C16orf57 gene. (unknown function)
Freeman-Sheldon syndrome– Autosomal dominant
Neanderthal genome
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Mutations with enhanced
resistance to killing by
chicken heterophils,
reflecting avian host
adaptive evolution.
De novo assemblies of microbes, BACs
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Sequencing whole genomes
Craig Venter
$20M+
Capillary
electrophoresis
2006
Watson
$2M
454
2007
3+ genomes
$200K/genome
GA
2008
Personal genome
$48K/genome
GAIIx
2009
Human genome
$10K/genome
HiSeq2000
2010
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Homozygous deletion by paired-end sequencing
From Ahn et al, Genome Res 2009
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Heterozygous deletion by paired-end sequencing
From Ahn et al, Genome Res 2009
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Metagenomics
Metagenomic study of the oral
microbiota by Illumina high-
throughput sequencing.
Metagenome
90%
Human genome
10%
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Summary
The decrease in cost of sequencing has revolutionized
genomics
Aneuploidy at higher sensitivity and lower cost than any
existing technology
Copy number variations without any prior assumptions,
with higher resolution and sensitivity and lower cost than
CGH arrays
Discovery of SNVs, indels, structural variation in either a
fraction of the genome (by targeted sequencing) or in the
whole genome at surprisingly low cost (from $1,000 to
$10,000/sample)
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Thank you