session 2 basic nutrients
DESCRIPTION
TRANSCRIPT
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“a substance that must be consumed as part of the diet to provide a source of energy, material for growth, or substances to regulate growth or energy production.”
- Bantam Medical Dictionary
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“a raw or processed agricultural commodity or other nutrient source”
- SACN, 5- SACN, 5thth Ed. Ed.
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Essential “Non-dispensable”Body cannot produce
Non-Essential “Dispensable”
The body can produce
Conditionally EssentialRequired during certain physiologic
or pathologic conditions
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Supply energyCofactors in metabolic chemical
reactionsTransport substances throughout
bodyRegulate body temperatureImpact food palatabilityForm structural components of
the body
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WaterWater CarbohydratesCarbohydrates ProteinProtein FatFat MineralsMinerals VitaminsVitamins Antioxidants*
EnergyProducingNutrients
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Most important nutrientBody composition ~ 70% of fat-free body
weightFunctions:
Solvent Transports materials Chemical reactant (hydrolysis) Supports blood volume and pressure Regulates body temperature Provides shape to body
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How Water Gets Into an AnimalHow Water Gets Into an AnimalMetabolizableMetabolizable IngestedIngested
Breakdown of Breakdown of carbohydrates, protein, carbohydrates, protein, fat as used for energyfat as used for energy
Free WaterFree Water
Routes of Water LossRoutes of Water Loss
Urine, Feces, Respiration, Perspiration, Milk, Urine, Feces, Respiration, Perspiration, Milk,
* Loss of 15% results in death
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Water Content Varies by Water Content Varies by NutrientNutrient
CarbohydrateCarbohydratess
0.6 grams of water per gram 0.6 grams of water per gram of carbohydrateof carbohydrate
ProteinProtein 0.4 grams of water per gram 0.4 grams of water per gram of proteinof protein
FatFat 0.2 grams of water per gram 0.2 grams of water per gram of fatof fat
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What types of things influence the amount
of water an animal
needs?
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Species
Environment (climate, air movement, etc.)
Food type and source
Diet composition (high protein, fiber, mineral salts)
Life stage and lifestyle
Water quality
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Total Dissolved Solids- Index for measuring water quality- More dissolved solids, decreased water
quality- 6 most common minerals present in water are
chloride, sodium, calcium, magnesium, sulfates and bicarbonate
- Other factors: pesticides, pathogens, bacteria, algae, protozoa
- < 2,500 mg/L dissolved solids desired
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Energy: “the ability to do work” -SACN, p.26
Calorie: amount of heat required to raise the temperature of 1 gram of water 1°C.
Kilocalorie = 1000 calories
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All living cells require energy
After water, energy is most critical requirement in nutrition
Carbohydrates, Protein, Fat provide energy from the diet
Not all of consumed energy is used
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GROSSENERGY(GE)
DIGESTIBLEENERGY(DE)
METABOLIZABLEENERGY(ME)
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Gross Energy (GE)
Total amount of heat produced when feed is burnt completely
GE determines total energy content of a feed, but doesn’t equal amount of energy available to the animal
GROSSENERGY(GE)
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Digestible Energy (DE)
the energy remaining after the energy lost in feces is subtracted from the gross energy
Measure of the energy absorbed from the feed after consumption
Not a true measure, some energy is from tissue sloughing from the GI tract
DIGESTIBLEENERGY(DE)
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Digestible Energy
DIGESTIBLE ENERGY = Gross Energy – Fecal energy losses
Image source: Dr. Geneva Acor, Hill’s Pet Nutrition, Inc.
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Metabolizable Energy (ME)
Energy available to the animal after energy from feces, urine, and gases has been subtracted from the gross energy
More accurate than DE for estimating amount of energy available to animal
Expensive: feeding trials
METABOLIZABLEENERGY(ME)
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Metabolizable Energy
METABOLIZABLE ENERGYMETABOLIZABLE ENERGY = Gross Energy – Fecal, Urine, Gas = Gross Energy – Fecal, Urine, Gas losseslosses
GrossEnergy
Digestible Energy
Image source: Dr. Geneva Acor, Hill’s Pet Nutrition, Inc.
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Most accurately predicts amount of energy available to the animal
Has been determined on a few feedstuffsUsed widely in formulating diets for ruminant
species
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Method for estimating energy content in a feed
Food is fed and all dietary components are measured
Amount of dietary components in feces is measured
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Digestible crude protein+ digestible crude fiber+ digestible nitrogen free extract ( starches
and sugars)+ 2.25 X Digestible ether extract ( fat)TOTAL DIGESTIBLE NUTRIENTS
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WHAT CHARACTERISTI
CS OF A FEED MIGHT AFFECT
ITS DIGESTIBILITY?
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Maturity of plant material- as plants mature, an undigestible material called lignin increases in plant cell walls
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Energy producing nutrient
Composed of carbon (C), hydrogen (H), oxygen (O) – CH2O
Includes sugars, glycogen, starch and cellulose
Mainly in plants (~75% of solid plant material)
Cell layer is cellulose; Starch is the energy source
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Monosaccharides CH2O GLUCOSE
Glucose & fructose most common
Glucose is immediate source of energy for cellular reactions i.e. tissue repair, muscle contractions, nerve transmissions
Body continually supplies glucose to blood from stored compounds in liver (glycogen)
Extremely Important
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Disaccharides (CH2O)2
Short chain of 2 CHO molecules
Lactose (milk sugar); Sucrose (table sugar)
Plants convert their stores of carbohydrates to sucrose for easy transport through the plant
Proteins often have attached disaccharides
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Polysaccharides (CH2O)3
Includes starches, glycogen and celluloseThe most abundant of all carbohydrates
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Starches- from plants, broken down into glucose subunits
Glycogen- storage form of glucose in animal cells, stored in liver and muscle, broken down to release glucose
Cellulose- comprises majority of plant cell wall, insoluble, digested by bacteria in the herbivore digestive tract
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Energy producing nutrient
Found in highest concentration of any nutrient except water in all living organisms
Composed of carbon (C), hydrogen (H), oxygen (O), and nitrogen (N) (and sometimes sulfur (S) and phosphorus (P)
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WHAT FUNCTIONS DO PROTEINS PERFORM
IN THE BODY?
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Functions
Tissue BuildingHormonesGrowthEnzymesImmune SystemBlood cells
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Made up of amino acid (AA) sub-units
Definition:Small organic compound that consists of an amino group (NH2) on one end and a carboxyl group (COOH) on other plus a special group that defines the individual amino acid
AA are linked together by peptide bonds
2 linked bonds = dipeptide
3+ linked bonds = polypeptide
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Synthesized by plants, rumen microorganisms
Non-herbivores get AA in diet
Absorbed in the anterior small intestine
Most protein in plants and animals composed of only 20 amino acids
10 Essential Amino Acids (11 for cats)
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Essential Amino AcidsEssential Amino Acids
Phenylalanine Histidine
Valine Arginine
Tryptophan Lysine
Threonine Leucine
Isoleucine
Methionine Taurine*Cats only
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Requirement for monogastric and avian species is for amino acids
High needs for: young, rapidly growing gestating and lactating animals surgery or trauma certain pathologic conditions
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Protein DeficiencyPoor growth rate in young; weight loss in
adultsExcess sheddingHigh neonate mortality; reduced egg
production, infertilityCauses of Protein DeficiencyCauses of Protein Deficiency
Inadequate food intakeInadequate food intake Poor quality proteinPoor quality protein Maldigestion or malabsorptionMaldigestion or malabsorption Protein losing enteropathy, NephropathyProtein losing enteropathy, Nephropathy Common in catteries and kennels Common in catteries and kennels
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Uncommon due to cost of feedUsed for energy or stored as fatChronic excess stresses kidneysExcretion of urea increases nitrogenous
wastes that build up in bloodstreamWhen urea or other non-protein sources are
fed to ruminants, toxicity and death occur if diet has insufficient carbohydrates
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Energy producing nutrient
Insoluble in water
Include fat, oils, complex sterols
Comprised of carbon (C), hydrogen (H) and oxygen (O)
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Fat is solid at room temperature
Oil is liquid at room temperature
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Functions
InsulationAbsorption of fat-soluble vitamins Hormone synthesisStructure to cell membraneMoisture to skinPalatability
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Accounts for most of body’s stored energy“Fat reserves”
Fat provides twice as much energy as CHO and protein
Source of essential fatty acids Hydrophilic, absorbs water and takes up
more space in the body
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Definition:A chemical unit that occurs naturally, either singly or
combined, and consists of strongly linked carbon and hydrogen atoms in a chain-like structure
Important for lipid-protein structure of cell membrane
Linoleic acid and Linolenic acid are essential for mammalian species. (Both are Omega 6 fatty acids)
Arachadonic acid is required in cats
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Important to brain developmentIam’s - “How to Grow Smart Puppies”™
Beneficial in inflammatory conditions
Common sources found in feeds:Flaxseed, linseed, menhaden fish, animal fat,
evening primrose oil, black currant oil
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Signs of EFA DeficiencySigns of deficiency: scaly skin, necrosis of tail,
reduced reproduction, edema, hemorrhaging, poor feathering in chickens
- Seen in pigs, chickens, calves, dogs, mice and guinea pigs
- Rare in ruminants, despite a fat-free diet, because the microbes produce adequate amounts of EFA
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1. Neutral fats2. Phospholipids3. Sterols4. Carotenoids5. waxes
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Most abundant fat in living thingsIncludes saturated fats and unsaturated fatsSaturated-have no shared carbon bondsUnsaturated- one shared bond between
carbonsPolyunsaturated-two or more shared bonds
between carbons
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Form cell membranes- one hydrophilic end and one hydrophobic end
Fatty acid portion ( tail) is hydrophobic and not water soluble
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Lipids that have no fatty acidsOccur in cell membranesInclude sex hormone ( estrogen, testosterone)Cholesterol, bile salts and sterols,
( biologically important)1.cholesterol- most common sterol in animal
cell tissues2.Bile salts- role in fat digestion in small
intestine
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Red and yellow pigment cells of all plantsClassified as lipids because of insolubility in
water and oily consistencyVitamin A comes from splitting pigment
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Long chain fatty acids tightly packed Firm consistency: repel waterHelp form the cuticle on plants and leavesKeeps feathers of water fowl clean and dry
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Monogastrics1.Primary site is small intestine2.Bile and pancreatic lipase3.If large amount of fat is mobilized for energy,
ketones form and excessive amounts are dumped into urine, milk and lungs
Ruminants- microbes convert unsaturated fats to saturated fats
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Small, organic compounds required by body for normal function
Cofactors in ezymatic reactions
Classified as fat solublefat soluble or water solublewater soluble - Absorbed through variety of ways
Important in milk – especially colostrumcolostrum
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Different for each vitamin
Vision Blood clotting factors Immune defense Epithelial tissue Bone mineralization and
resorption Neural transmission
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Vitamin AVitamin CPotassium
ZincVitamin E
IronMagnesium
CalciumVitamin KSelenium
Vegetable OilTuna, red meat
Citrus, green peppersCarrots, pumpkinBeef, Brazil nutsSardines, milkYogurt, lentils
SoybeansNuts, bananas
Cauliflower, broccoli
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Vitamins A, D, E, K Higher potential for toxicity than water
soluble vitamins
Require fat and bile salts to be absorbed
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Important part of visual proteins
Key for epithelial tissues (skin, lining of body cavities)
Beneficial to immune system
Signs of deficiency: poor coat, retarded growth, anorexia
Signs of toxicity: anorexia, “Cervical spondylosis” in
cats
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Calcium/Phosphorus balanceKey in bone resorption and mineralizationAbsorbed as ergosterol, converted to
calciferol (D2) in skin
Kidneys convert D2 to different compound used for calcium absorption
Signs of deficiency: Rickets, posterior paralysis
Signs of excess: anorexia, kidney stone, FORLs
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Antioxidant “Tocopherol”Alpha tocopherol: active in bodyMixed tocopherol: active in food
Protects cell membranes
Signs of deficiency: sterility in males, steatitis in cats, immunodeficiency
Signs of toxicity: Increased clotting time
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Vital to blood clotting proteins
Synthesized by microbes in ruminant gut
Deficiency is rare in all species except poultry
Signs of deficiency: increased clotting time, hemorrhaging
Sign of excess: minimally toxic
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B-Vitamins, Vitamin C
Minimally toxic, due to excretion from the body in urine
Key as cofactors in enzymatic reactions
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Thiamin (BThiamin (B11)) Pyroxidine (BPyroxidine (B66))
Riboflavin (BRiboflavin (B22)) Biotin (BBiotin (B77))
Niacin (BNiacin (B33)) Folic Acid (BFolic Acid (B99))
Pantothenic acid Pantothenic acid (B(B55))
Cobalamin (BCobalamin (B1212))
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Functions varied among forms of Vitamin B
No single food source has enough vitamin B - must be gained from multiple sources
Riboflavin deficiency is rare
Niacin deficiency results in pellagra with dermatitis; dementia
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Antioxidant
Collagen synthesis
Important in Carnitine synthesis
Signs of deficiency: scurvy Signs of toxicity: rare
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Natural component of animal cells
Most carnitine (~98%) in body lives in skeletal and cardiac muscle
Helps transport fatty acids into cells
Helps build lean tissue
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In nutrition, mineral means all inorganic elements in a food
Major portion of body fluids (electrolytes)
Important in enzymatic reactions
“Ash”
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Macro MineralsMacro Minerals
CalciumCalcium
PhosphorusPhosphorus
SodiumSodium
MagnesiumMagnesium
PotassiumPotassium
ChlorideChloride
SulfurSulfur
Micro MineralsMicro Minerals
ChromiumChromium
CobaltCobalt
CopperCopper
FluorineFluorine
IronIron
IodineIodine
ManganesManganesee
MolybdenuMolybdenumm
NickelNickel
SeleniumSelenium
SiliconSilicon
ZincZinc
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Largest amounts found in skeletal system
Provide structural rigidity
Some functions:Iron is essential part of hemoglobinIodine is component of thyroid
hormoneSodium is key to body’s pH level
Of total minerals in animal’s body…Ca = 46% and P = 29%
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Absorbed from the GI tract
Fat sometimes chelates certain minerals, thereby reducing their availability to animal
Young are more efficient than old at absorbing minerals
Requirements vary by species
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Definition:
“an especially reactive atom or group of atoms that has one or more unpaired electrons; produced in the body by natural biological processes or introduced from an outside source (as tobacco smoke, toxins, or pollutants) and that can damage cells, proteins, and DNA by altering their chemical structure.”
- Merriam-Webster Dictionary
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DNA
Mitochondria
Attack cell membranes,Attack cell membranes,DNA & MitochondriaDNA & Mitochondria mutations in DNAmutations in DNA changes fluidity of changes fluidity of
cell membrane cell membrane
Cause Cause oxidative stressoxidative stress in the bodyin the body
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Creates DNA damageDamage accumulates over course of animal’s lifeInjures cellsResponsible for lots of chronic illnesses
Cancer, Kidney disease, Cardiovascular disease, Cognitive Dysfunction Syndrome, etc
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PollutionRadiation
MetabolismChemicals
Food Additives
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Mitochondria“Powerhouseof the Cell”
DamagedMitochondria
Body’s Defense
sBody’s Defense
s
Body’s Defense
s
Body’s Defense
sXX
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Definition:
“a substance that inhibits oxidation or reactions promoted by … free radicals”
-Merriam-Webster Dictionary
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Inactivate free radicals
Improve cellular efficiency
Work best in conjunction with other antioxidants
(help to regenerate each other)
Some are active in food; some are active in the body
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Fights oxidation in the food
Carotenoids & Flavonoids (from Vitamin A)
Mixed Tocopherols (form of Vitamin E)Vitamin CEthoxyquin
Commonly marketedCommonly marketedin dog and cat foodin dog and cat food
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Fight oxidation in the animal’s cells
Alpha tocopherols (form of Vitamin E)Ascorbic AcidSeleniumLipoic AcidBeta CaroteneCarotenoidsFlavanoids
“Cowboy”
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SMALL ANIMAL NUTRITION: ENERGY CALCULATIONS &
LIFE STAGE/LIFE STYLE NUTRITION