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    Sexual Reproduction of the

    Flowering Plant

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    Review the plant life cycle

    1

    4

    2

    pollen is

    transferred

    3

    After fertilization

    flower withers

    seeds disperse

    and germinate

    into new plant

    seeds develop

    in ovary

    4

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    Structure of the flower

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    Structure of the flower

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    Carpel

    Petal

    Anther

    Filament

    Stamen

    Stigma

    Style

    Ovary

    OvuleSepal

    Structure of the flower

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    Function of floral parts

    Sepal : To protect the

    flower (and to prevent it

    from drying out

    Petals : To attractinsects to the flower for

    pollination

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    Function of floral parts

    Stamen : To produce

    the pollen grains in the

    anthers. (Each pollen

    grain produces twomale gametes, one of

    which can fertilise an

    egg cell)

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    Function of floral parts -Stamen

    Anther

    Produces pollen

    Filament

    Holds the anther in place

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    Function of floral parts

    Carpel : To produce

    the ovules (Each ovule

    contains an egg cell

    inside an embryo sac)

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    Function of floral parts - Carpel

    Stigma

    Where pollen landsafter pollination

    Style

    Pollen travels down this

    Ovary

    Contains ovules

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    Pollination

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    Pollination

    Transfer of pollen from the anther to the stigma of

    a flower of the same species

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    Pollination

    Self pollination

    Transfer of pollen from

    an anther to a stigma

    of the same plant

    Cross pollination

    Transfer of pollen from

    the anther to the stigma

    of a different plant ofthe same species

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    Methods of pollination

    Animal Pollination

    Wind Pollination

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    Petals brightly

    coloured, scented with

    nectaries

    Small amounts of stickypollen

    Anthers inside petals

    Stigmas sticky, inside

    petals

    Adaptations for animal (insect) pollination

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    Petals small, not

    coloured brightly

    Anthers outside petals

    Stigmas large, feathery

    and outside petals

    Pollen Large numbers,

    light, dry and small

    Adaptations for wind pollination

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    Adaptations for wind pollination

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    Fertilisation

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    Fertilisation

    Fertilisation is the fusion of the male (n) and

    female (n) gametes to produce a zygote (2n)

    The pollen grain produces the male gametes

    Embryo sac produces an egg cell and polar

    nuclei

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    The pollen grain

    produces the male

    gametes

    Embryo sacproduces polar

    nuclei and an egg

    cell

    Polar nuclei

    Embryo sac

    Egg cell

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    Stigma

    Style

    Ovary

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    Embryo Sac

    Polar nuclei

    Egg Cell

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    Pollen Grain

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    Pollen Tube

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    Tube Nucleus

    Generative Nucleus

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    Tube nucleus

    disintegrates

    Mitotic division

    of generativenucleus to form

    2 male gametes

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    1 Male gamete fuseswith the 2 polar nuclei

    to form the triploid

    endosperm nucleus1 male gamete

    fuses with the egg

    nucleus to form the

    diploid zygote

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    3N endosperm

    nucleus

    2N Zygote

    Double

    fertilisation

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    Seed formation

    Endospermic & Non-Endospermic

    Monocots & Dicots

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    Seed Formation

    The zygote grows

    repeatedly by mitosis

    to form an embryo

    An embryo consists ofa plumule (future

    shoot), a radical (future

    root) and cotyledons

    (food stores needed forgermination)

    3N

    endosperm

    nucleus

    2N Zygote

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    Seed Formation

    The endosperm

    nucleus (3N) divides

    repeatedly to form the

    endosperm in

    endospermic seeds.

    This endosperm acts as

    a food store for the

    developing seed

    e.g. maize

    3N

    endosperm

    nucleus

    2N Zygote

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    Seed Formation

    In non-endospermic

    seeds the endosperm is

    used up in the early

    stages of seed

    development so the

    food is stored in the

    cotyledons

    e.g. bean

    3N

    endosperm

    nucleus

    2N Zygote

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    Endosperm

    Food storefor

    developing

    embryo

    Embryo

    Plumule,radicle,

    cotyledons

    Integuments, becomes the seed coat

    Seed Formation

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    If all the

    endosperm is

    absorbed by

    the developing

    embryo theseed is a non

    endospermic

    seed e.g. broad

    bean

    Seed Formation

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    If all the

    endosperm isnot absorbed

    by the

    developing

    embryo theseed is an

    endospermic

    seed e.g.

    Maize

    Seed Formation

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    Seed types and structure

    Seed

    embryo

    Plumule (immature shoot)

    Radicle (immature root)

    Cotyledon (food supply

    or seed leaf)

    endosperm Food store

    All seeds

    In some seeds

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    Endosperm

    Seed coat

    (testa)

    Cotyledon

    Plumule will

    develop into a

    new shoot

    Radicle will develop

    into a new root

    Endospermic Seed e.g. Maize

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    Seed coat

    (testa)

    Cotyledon

    Plumule

    Radicle

    Non-Endospermic seed e.g. Broad Bean

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    e.g. Broad Bean e.g. Maize

    Plumule

    Radicle

    Cotyledon

    Endosperm

    Nonendospermic and Endospermic seed

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    Classification of seeds

    Classified according to two features:1. Number of cotyledons (Seed leaves)

    Monocotyledon one cotyledon

    E.g. Maize

    Dicotyledon - Two cotyledons

    E.g. Broad bean

    2. Presence of endosperm Present Endospermic e.g. maize

    Absent Non-endospermic e.g. broad bean

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    Broad BeanNon-Endospermic Dicot

    Testa2 Cotyledons

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    Fruit

    Fruit formation

    Seedless fruitsFruit and seed dispersal

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    Fruit Formation

    The ovule becomes the seed

    The ovary becomes the fruit

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    Fruit Formation

    A fruit isa mature

    ovary that may contain

    seeds

    The process of fruit

    formation is stimulated

    by growth regulators

    produced by the seeds

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    Seedless Fruits

    Can be formed in two ways

    1. Genetically

    Either naturally or by

    special breedingprogrammes

    e.g. seedless oranges

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    Seedless Fruits

    2. Growth regulatorse.g. auxins

    If large amounts ofgrowth regulatorsare sprayed onflowers fruits mayform withoutfertilisation

    e.g. seedlessgrapes

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    Fruit and seed dispersal

    Need for dispersal

    Minimises competition

    for light, water etc.

    Avoids overcrowding Colonises new areas

    Increases chances of

    survival

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    1.

    Wind2. Water

    3. Animal

    4. Self

    Types of dispersal

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    Methods of dispersal

    1. Wind

    Sycamore and ash

    produce fruit with wings

    Dandelions and thistlesproduce fruit with

    parachute devices

    Both help the dispersethe seeds more widely

    using wind

    h d f d l

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    Methods of dispersal

    2. Water

    Light, air filled fruits

    that float away on

    water E.g. coconuts, water

    lilies

    h d f di l

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    Methods of dispersal

    3. Animal

    Edible fruit

    Animals attracted

    to bright colours,smells and food

    Seed passesthrough digestive

    system unharmed E.g. strawberries,

    blackberries, nuts

    M h d f di l

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    Methods of dispersal

    3. Animal

    Sticky fruit

    Fruits with hooks

    that can cling to thehair of an animaland be carriedaway

    E.g. burdock,goose grass

    M h d f di l

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    Methods of dispersal

    4. Self

    Some fruits explode open when they dry out

    and flick the seed away

    E.g. peas and beans

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    Dormancy and germination

    D (d fi i i )

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    Dormancy (definition)

    A resting period when seeds undergo no

    growth and have reduced cell activity or

    metabolism

    D ( d )

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    Dormancy (advantages)

    Plant avoids harsh

    winter conditions

    Gives the embryo

    time to develop Provides time for

    dispersal

    A li i i i l d h i l

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    Application in agriculture and horticulture

    Some seeds need a period of cold before

    they germinate

    It may be necessary to break dormancy in

    some seeds before they are planted foragricultural or horticultural purposes

    This can be done by placing them in the

    fridge before they are planted

    G i i

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    Germination

    The re-growth of the

    embryo after a period

    of dormancy, if the

    environmentalconditions are

    suitable

    G i i F

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    GerminationFactors necessary

    Water

    Oxygen

    Suitable temperature

    Dormancy must be complete

    G i i F

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    GerminationFactors necessary

    Water

    Activates the

    enzymes

    Medium forgermination

    reactions e.g.

    digestion

    Transport medium

    for digested

    products

    G i ti F t

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    GerminationFactors necessary

    Oxygen

    Needed for aerobic

    respiration

    Suitable temperature

    Allows maximum

    enzyme activity

    E t i G i ti

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    Events in Germination

    Digestion

    Of stored food in endosperm and cotyledon

    Respiration

    To produce ATP to drive cell division

    Events in germination cease when the plantsleaves have developed and the plant has

    started to photosynthesise

    E t i G i ti (d t il)

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    Events in Germination (detail)

    Water is absorbed Food reserves are digested

    Digested food is moved to the embryo

    New cells are produced using amino acids Glucose is turned into ATP to drive cell division

    Radicle breaks through the testa

    Plumule emerges above ground

    New leaves begin to photosynthesise

    E t i G r i ti

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    Events in Germination

    Plumule

    Radicle

    Cotyledon

    E nts in G rmin ti n

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    Events in Germination

    Plumule

    Radicle

    Changes in dry weight of seeds during germination

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    Mass drops initially due to respiration of stored food, but then

    begins to increase due to photosynthesis

    Drymassofseed(g)

    Time (days)

    Changes in dry weight of seeds during germination

    Changes in dry weight of seeds during germination

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    Food reserves in endosperm are transferred to the growing embryo

    Drymassofseed(g)

    Time (days)

    Embryo

    Endosperm

    Changes in dry weight of seeds during germination

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    Germination of broad bean (hypogeal)

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    Germination of broad bean (hypogeal)

    Germination of broad bean

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    Seed water is absorbed

    through the micropyle

    Ground

    Germination of broad bean

    Germination of broad bean

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    The testa splits

    Radicle emerges

    Germination of broad bean

    Germination of broad bean

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    Radicle continues to grow

    Plumule emerges

    Germination of broad bean

    Germination of broad bean

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    The plumule is

    hooked to protect

    the leaves at the tip

    Epicotyl

    Germination of broad bean

    Germination of broad bean

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    The plumule

    grows above

    the surface of

    the soil

    Lateral rootsdevelop

    Germination of broad bean

    Germination of broad bean

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    Plumule

    straightens and

    the leaves open

    out

    Throughout Hypogeal

    germination the

    cotyledons remain

    below the ground

    Germination of broad bean

    Germination of sunflower (Epigael)

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    Seed

    water isabsorbed through the

    micropyle

    Germination of sunflower (Epigael)

    Germination of sunflower

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    Radicle

    emerges

    Germination of sunflower

    Germination of sunflower

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    Hypocotyl Hook

    Germination of sunflower

    Seed coat

    discarded Germination of sunflower

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    Radicle grows

    downwards

    discarded

    Cotyledons

    Germination of sunflower

    Germination of sunflower

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    Leaves emerge

    Cotyledons wither

    Germination of sunflower

    In Epigeal germinationthe cotyledons rise

    above the ground

    Learning Check

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    Learning Check

    Outline the main stages of sexualreproduction in plants

    Review the plant life cycle

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    1

    4

    2

    pollen is

    transferred

    3

    After fertilization

    flower withers

    seeds disperse

    and germinate

    into new plant

    seeds develop

    in ovary

    4

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    Asexual Reproductionin Plants

    Vegetative Propagation

    Definition

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    Definition

    Asexual reproduction does not involve the manufacture or union of sex

    cells or gametes e.g. binary fission,

    fragmentation, spore formation and budding It involves only one parent and offspring are

    genetically identical (have the same genetic

    content) to the parent

    Vegetative Propagation

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    Vegetative Propagation

    A form of asexual reproduction in plants

    Does not involve gametes, flowers, seeds or

    fruits

    Offspring are produced by a single plant(genetically identical to parent)

    Can happen naturally or it can be done

    artificially

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    Vegetative Propagation

    Natural

    e.g. runners, tubers, plantlets, bulbs

    What happens?

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    What happens?

    Part of the plant becomes separated from theparent plant and divides by mitosis to grow

    into a new plant

    As a result the offspring are geneticallyidentical to the parent

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    Parts of the parent plant may be speciallymodified for this purpose:

    1. Stem

    2. Root

    3. Leaf

    4. Bud

    1 Modified Stems

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    1. Modified Stems

    Runners

    horizontal, running over

    the soil surface

    terminal bud of therunner sends up new

    shoots

    e.g. strawberry,

    creeping buttercup.

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    Creeping buttercup

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    Modified Stem (continued)

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    ( )

    Stem Tubers

    swollen underground

    stem tips

    buds (eyes) produce

    new shoots

    e.g. potato

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    2 Modified Roots

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    2. Modified Roots

    Root Tuber

    swollen fibrous roots

    the tuber stores food,

    but the new plant

    develops from a side

    bud at the base of the

    old stem

    e.g. dahlia, lessercelandine

    Note:

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    Note:

    Tap Roots e.g. carrotand turnip, are swollen

    roots for food storage in

    biennial plants they

    are not reproductiveorgans

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    4. Modified Buds

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    Bulbs

    A bulb contains an

    underground stem,

    reduced in size Leaves are swollen

    with stored food

    e.g. onion, daffodil,tulip

    4. Modified Buds

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    Bulbs

    The main bud

    (apical bud) will

    grow into a newshoot)

    The side buds

    (lateral buds) will

    also grow into new

    shoots

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    Comparison of reproduction byseed (sexual) and by vegetative

    propagation (asexual)

    Advantage to seed formation

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    Sexual (seed) Asexual (vegetative)

    Cross pollination ensures

    variation (allows evolution)

    No variations can be

    advantage in commercial

    horticulture

    More resistant to disease All plants are of same speciessusceptible to disease

    Dispersal reduces competition Overcrowding and competition

    Seeds can remain dormant and

    survive unfavourable conditions

    No seeds formed no

    dormancy

    Advantage to vegetative propagation

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    Sexual (seed) Asexual (vegetative)

    Complex process Simple process

    Depends on outside

    agents for seed dispersal

    No outside agents

    needed

    Slow growth of young

    plants to maturity

    Rapid growth

    Wasteful e.g. petals,pollen, fruit

    No waste

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    Vegetative propagation

    Artificialused by gardeners to propagate plants

    e.g. cuttings, layering, grafting and budding

    Cuttings

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    Cutt gs

    Parts of a plant (usuallyshoots) removed from

    plant allowed to form

    new roots and leaves

    rooted in water, well-watered compost, or

    rooting powder

    e.g. busy lizzie,

    geranium

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    Grafting

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    Grafting

    Part of one plant (scion)is removed and

    attached to a healthy,

    rooted part of a second

    plant (stock) Useful qualities from

    both plants combined

    into one e.g. rose

    flower and thorn-lessstem

    e.g. apple trees

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    Layering

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    A branch of a plant is bent over and pinned tothe earth at a node

    When roots develop the branch is separated

    from the parent plant. Useful for the propagation of woody plants

    e.g. blackberry, gooseberry.

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    Micropropagation (Tissue Culture) (1/3)

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    Cells removed fromplant and grown as a

    tissue culture in a

    special medium

    Growth regulators andnutrients added so that

    growing cells form a

    group of similar cells

    called a callus

    Micropropagation (Tissue Culture) (2/3)

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    Different growth regulators are then added so thatthis tissue develops into a plantlet

    Plantlet can be divided up again to produce many

    identical plants

    Entire plant can be grown from a small piece ofstem, leaf or root tissue

    Used in mass production of house plants and crops

    such as bananas and strawberries

    Micropropagation (Tissue Culture) (3/3)

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    Provides a largernumber of plants more

    quickly than cuttings.

    Can be used to check

    cells for a particularfeature e.g. resistance

    to chemicals or a

    particular disease

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    Cloning

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    All offspring genetically identical - produced

    asexually

    Clones are produced by mitosis All the offspring from the various methods of

    vegetative reproduction (both natural and

    artificial) mentioned are examples of clones

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    END