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Signal Transduction Society
(STS)
20th Joint Meeting
Signal Transduction
Receptors, Mediators and Genes
Leonardo Hotel Weimar
November 9 - 11, 2016
Table of Contents
1
Table of Contents
Welcome Note 2
Time Table 3
Organizers 5
Sponsors 6
Meeting Information 8
Agenda of the General Assembly of the STS 10
Workshop Program
Workshop A Focus: Cells in Motion 11
Workshop D G Protein-coupled Receptors 12
Workshop E Receptor and Redox Signaling 13
Workshop F Immune Cell Signaling 14
Workshop B Focus: Molecules in Motion 16
Workshop G Signaling in Infection and Inflammation 17
STS/CCS Honorary Medal Award Ceremony 18
Workshop C Focus: Organelles in Motion 19
Workshop H Differentiation, Stress and Death 20
Poster-Session Program
Session I PA1 - PD3 24
Session II PE1 - PL8 24
Addresses of Sponsors 28
Register of Presentations listed by Presenting Authors 30
Welcome Note
2
Dear Colleagues:
It is our pleasure to cordially welcome you to the 20th Joint Meeting 'Signal Transduction - Receptors, Mediators and Genes' in the Leonardo Hotel in Weimar.
This year’s meeting is jointly organized by the STS in association with our long-standing partners, the German Societies for Immunology (DGfI), for Cell Biology (DGZ) and for Pharmacology (DGP) and the Society for Biochemistry and Molecular Biology (GBM). We once again thank the SFB 854 'Molecular Organisation of Cellular Communication in the Immune System' (chair: Burkhart Schraven, Magdeburg) for its contribution. This year, the Netherlands Society for Biochemistry and Molecular Biology (NVBMB) joins the meeting for the first time.
One successful concept in organizing a highly attractive meeting each year is to offer focused symposia presenting state-of-the-art science on changing topics. The special focus of this year’s meeting, 'Cells and Molecules in Motion', was jointly organized by the GBM study groups 'Biochemical Pharmacology and Toxicology' (Klaudia Giehl, Gießen) and 'Receptors and Signal Transduction' (Karlheinz Friedrich, Jena), the DGZ study group 'Signal Transduction' (Ralf Hass, Hannover), and the DGfI study group 'Signal Transduction' (Ingo Schmitz, Braunschweig and Friederike Berberich-Siebelt, Würzburg). We also thank the DGfI study group 'Biology of B Lymphocytes' (Jürgen Wienands, Göttingen) for financial and scientific support. Renowned invited experts will open each workshop with an introductory keynote lecture. All other oral presentations are selected from the submitted scientific contributions. Moreover, the poster sessions provide the opportunity for literally all other participants to introduce their work to the plenum in the framework of the traditional 'one minute-one transparency' presentations.
We are pleased and especially honored to announce the award lecture given by Prof. Dr. Tak Wah Mak (University of Toronto, Canada) who will receive this year’s STS/CCS Honorary Medal for his lifetime contributions and milestone discoveries in the fields of immunology, signal transduction and cancer biology. The laudation will be
given by Klaus Pfeffer from the University of Düsseldorf.
Our particular thanks go to all industrial sponsors who support the STS Meeting 2016. We especially acknowledge the continuous extra support by Biomol GmbH and New England Biolabs GmbH / Cell Signaling Technology by sponsoring the STS-Science-Award and the STS 20th Anniversary Special-NEB/CST-Award, respectively. Without the financial input by our industrial partners, it would not be possible to keep the conference fees at a moderate level. We strongly encourage all participants to visit the sponsors’ exhibition, to participate in the 'Crosstalk Puzzle' and to attend the informative plenary talks by our industrial partners. Please note that the 'Crosstalk Puzzle' raffle will be part of the STS-Anniversary Conference Party. Please be aware that prizes, stipends and awards can only be received in person.
The General Assembly of the Signal Transduction Society will take place on Thursday, November 10th, 2016, starting at 2pm. All members of the STS are cordially invited to discuss the past, presence and future of the Society. Those of you who want to join the STS will be able to do so by registering online at http://www.sigtrans.de.
The aim of the STS to promote and encourage the exchange of scientific ideas and to foster the education of young scientists in the highly dynamic field of cellular signal transduction has been pursued successfully for 20 years now. Thus, we think this is a good reason to celebrate our anniversary with all of you with a Conference Party on Thursday, November 10th after dinner in the lecture hall. Join us, enjoy the evening with the 'Crosstalk Puzzle' raffle to start with, continue on the dance floor with live music presented by the local band ‚Paolo Macho’, and enjoy the STS-cocktail and other beverages in a relaxed atmosphere.
We wish you a very pleasant stay in Weimar, a memorable anniversary party and of course a most interesting, stimulating and successful Signal Transduction Meeting.
Klaudia Giehl, Katharina Hieke-Kubatzky, Ottmar Janssen, Detlef Neumann
20th Joint Meeting Signal Transduction
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Time Table
Wednesday, November 9
08.00 Registration, Poster Mounting, Gathering
08.45 Welcome Note
09.00 Workshop A Focus: Cells in Motion
10.45 Coffee Break, Sponsors’ Exhibition, Poster Preview
11.15 Workshop D G Protein-coupled Receptors
13.00 Lunch Break
14.00 Workshop E Receptor and Redox Signaling
15.30 Coffee Break, Sponsors’ Exhibition, Poster Preview
16.00 Workshop F Immune Cell Signaling
17.45 Coffee Break, Sponsors’ Exhibition, Poster Preview
18.15 Poster Presentation I
PA1 - PD3 'One Minute - One Transparency'
19.00 Poster Discussion, Sponsors' Exhibition
20.00 Dinner
Time Table
4
Thursday, November 10
08.30 Workshop B Focus: Molecules in Motion
10.30 Coffee Break, Sponsors’ Exhibition, Poster Preview
11.00 Workshop G Signaling in Infection and Inflammation
13.00 Lunch Break
14.00 General Assembly of the STS
15.30 Coffee Break, Sponsors’ Exhibition, Poster Preview
16.00 STS/CCS Honorary Medal Award Ceremony
17.45 Coffee Break, Sponsors’ Exhibition, Poster Preview
18.15 Poster Presentation II
PE1 - PL8 'One Minute - One Transparency'
19.00 Poster Discussion, Sponsors' Exhibition
20.00 Dinner, Party, Crosstalk Raffle
Friday, November 11
09.00 Workshop C Focus: Organelles in Motion
10.30 Coffee Break, Sponsors’ Exhibition, Poster Preview
11.00 Workshop H Differentiation, Stress and Death
13.00 Lunch, Poster and Science Awards
15.00 End of Meeting
20th Joint Meeting Signal Transduction
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STSSignal Transduction Society
Gesellschaft für Signaltransduktion
GST
20thJoint Meeting
Signal Transduction:
Receptors, Mediators and Genes
Leonardo Hotel Weimar
November 9 - 11, 2016
Organizers: Klaudia Giehl, Gießen
Katharina Hieke-Kubatzky, Heidelberg
Ottmar Janssen, Kiel
Detlef Neumann, Hannover
Program Committee: Klaudia Giehl, Gießen
Katharina Hieke-Kubatzky, Heidelberg
Ottmar Janssen, Kiel
Frank Entschladen, Witten
Marcus Lettau, Kiel
Workshop Chairpeople
Grant Committee: Klaudia Giehl, Gießen
STS-Council
Program Booklet: Beate Lengl-Janßen, Kiel
Cornelius Bartke, Berlin
Webmaster: Cornelius Bartke, Berlin
STS - Offices: Ute Erhorn, Hannover
Beate Lengl-Janßen, Kiel
Sponsors
6
The 20th Joint Meeting 'Signal Transduction - Receptors, Mediators and Genes' is traditionally
organized as a Joint Meeting of the Signal Transduction Society (STS) with 'Signal Transduction'
study groups of the German Societies for Cell Biology (DGZ), for Immunology (DGfI), for
Pharmacology (DGP) and the Society for Biochemistry and Molecular Biology (GBM). Moreover,
individual workshops are co-organized by the DGfI study group 'Biology of B Lymphocytes', the
SFB 854 'Molecular Organisation of Cellular Communication in the Immune System' and the
Netherlands Society for Biochemistry and Molecular Biology (NVBMB).
As organizers, we are very grateful to all companies supporting the STS Meeting 2016. We
especially acknowledge all companies who support our 20th Anniversary by donating awards and
prizes, travel stipends as well as vouchers for the Conference Party. Thank you very much!
We strongly encourage all participants to meet the representatives at their booths. Without their
constant support and their scientific contributions, the Signal Transduction Meeting series would
not have been possible. For contact information, see page 139.
Sponsors of the STS Meeting 2016 are:
Acris Antibodies GmbH
Adipogen AG
Berthold Technologies GmbH & Co KG
Biomol GmbH
BioVendor GmbH
Biozol Diagnostica Vertrieb GmbH
Cisbio Bioassays
Eurogentec S.A.
Gilson International B.V.
HiSS Diagnostics GmbH
LI-COR Biosciences GmbH
MoBiTec GmbH
Molecular Devices (Deutschland) GmbH
New England Biolabs GmbH / Cell Signaling
Technology Inc.
OLS OMNI Life Science GmbH & Co. KG
PerkinElmer LAS (Germany) GmbH
PromoCell GmbH
Proteintech Europe Ltd
Sarstedt AG&Co
SERVA Electrophoresis GmbH
Sony Europe Ltd
tebu-bio GmbH
Thermo Fisher Scientific Inc.
Wako Chemicals GmbH
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'SILVER SPONSORS'
donate additional travel support for young scientists.
As a good tradition, each year the STS provides 10 travel grants to young fellows to attend the
meeting and present their data to the public. For the anniversary meeting, however, we had 15
applications and started to discuss how to select proper candidates. In parallel, we thought of
attracting some additional travel funding from our industrial partners. We have to admit that we
were quite overwhelmed by the instant and more than positive feedback. The STS council is very
grateful to all 'SILVER SPONSORS' who allow us to award a total of 15 stipends to talented
junior scientists at the General Assembly on Thursday, November 10th, 2016.
The 2016 'SILVER SPONSORS' are:
Meeting Information
8
The 20th Anniversary of the Joint Meeting
'Signal Transduction: Receptors, Mediators and Genes'
In the late 1990s, colleagues from various disciplines of molecular biosciences have become increasingly interested in the developing research area of signal transduction. In 1996, the speaker of the DGfI Study Group 'Signal Transduction' Ottmar Janssen teamed up with his colleague Ralf Hass from the DGZ to improve this situation and in spring 1997, Karlheinz Friedrich from the GBM joined to co-organize the first interdisciplinary conference on Signal Transduction in Germany at the Paul-Ehrlich-Institute in Langen. After 2 successful meetings at the Paul-Ehrlich Institute, the Meeting moved to Berlin and in 2001 found an almost ideal location at the Leonardo (former Hilton) Hotel in Weimar. Main purpose of the STS was and still is the development of an interdisciplinary forum for researchers who are deciphering signal transduction pathways. Although the overall ideas and the general concept of the meetings did not change so much, the STS was able to continuously improve its quality and outreach. We encourage investigators from all areas of signal transduction research to become members of the STS and to strengthen and shape the standing of this fascinating aspect of biomedical research.
To celebrate the 20th anniversary of our meeting series, the STS invites all of you to our Conference Party on Thursday, November 10th after dinner with drinks supported by 'SILVER SPONSOR'
and live music by
Paolo Macho! Live for you on stage
Discobeat, Electro Pop, Ska and Country Feeling. Punkrock by attitude and always very Sha-quedelique by heart!
Sometimes supported by confetti cannons, balloons filled swimming pools or soap bubbles, we don’t have time for Halligalli! We gonna have fun on stage and you all are invited to dance with us. This time Paolo Macho will be supported by Mr Reverend on the turntables playing his best Mixture of Funky Tunes to keep you dancing all night long.
Don’t forget to put on your dancing shoes!
PS: Don't call us Macho, call us Macho. Paolo Macho rhymes with Voll Karacho!
20th Joint Meeting Signal Transduction
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STSSignal Transduction Society
Gesellschaft für Signaltransduktion
GST
For information about the STS, membership applications,
STS membership benefits, upcoming events
and much more, visit our homepage at:
http://www.sigtrans.de
search for:
Signal-Transduction-Society
Agenda of the General Assembly of the STS
10
STSSignal Transduction Society
Gesellschaft für Signaltransduktion
GST
General Assembly of the Signal Transduction Society (STS)
Date: Thursday, November 10, 2016, 14.00 - 15.30
Location: Leonardo Hotel Weimar, Goethe Lecture Hall
President: Klaudia Giehl
Protocol: Frank Entschladen
Agenda:
1. Genehmigung der Tagesordnung / Adoption of the Agenda
2. Genehmigung des Protokolls der Mitgliederversammlung 2015 / Adoption of the
Protocol of the General Assembly 2015
3. Bericht der Präsidentin / President’s Report
4. Bericht zu den Finanzen / Treasurer’s Report
5. Entlastung des Vorstands / Exoneration of the Council
6. Wahlen: STS Vorstands- und Beiratsmitglieder / Elections: STS Council and
Advisory Board members
6.a NEU: Satzungsdurchbrechender Beschluss zur Aussetzung der Vorstandswahl
und zur Verlängerung der Amtszeit der amtierenden Präsidentin und des
amtierenden 2. Vizepräsidenten um 1 Jahr / NEW: Suspension of the elections of
STS council member and extension of the mandate of the president and past
president for 1 year
7. Ehrenmitglieder der STS / Honorary members of the STS
8. STS Meeting 2017
9. Mögliche Weiterführung des Journals CCS durch die STS / Potential adoption of
Cell Communication and Signaling CCS by the STS
10. Verschiedenes / Matters arising
11. Vergabe der STS-Stipendien / Awarding of Travel Stipends
The STS Council
Klaudia Giehl (Gießen), Katharina Hieke-Kubatzky (Heidelberg), Ottmar Janssen
(Kiel), Detlef Neumann (Hannover)
The STS Advisory Board
Friederike Berberich-Siebelt (Würzburg), Frank Entschladen (Witten), Karlheinz
Friedrich (Jena), Arnd Kieser (München), Lars-Oliver Klotz (Jena), Marcus Lettau
(Kiel), Ingo Schmitz (Braunschweig/Magdeburg)
Workshop Program
11
Workshop A
Focus: Cells in Motion
Wednesday, 09.11.2016 Chair: Annette Borchers, Marburg 09.00 – 10.45 Frank Entschladen, Witten
09.00h A1 Keynote:
Molecular mechanisms underlying actin-based cell migration
Faix J. Hannover
09.30h A2 Keynote:
Signaling to actin dynamics in cell and cell-in-cell invasion Grosse R. Marburg
10.00h A3 Akt isoforms in controlling the cell migration of lung and pancreatic
adenocarcinoma cells
Lammert A., Bier J., Jücker M., Giehl K. Gießen
10.15h A4 DigiWest multiplex protein profiling for comprehensive mapping of cell
transduction in materials from multiple species Treindl F., Beiter Y., Breitinger E., Döttinger A., Kling S., Hauessler R., Schneider J., Erdmann G., Sachse C., Templin M.F. Berlin
10.30h A5 Presentation by OLS OMNI Life Science GmbH & Co. KG:
Capture of cells in motion - the xCELLigence impedance-based assays for real-time and label-free measurement of cell proliferation, adhesion and migration: critical evaluation and caveats in data interpretation
Ungefroren H., Ketterlinus R., Schwengberg S. Bremen
10.45h Coffee Break / Sponsors' Exhibition
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Workshop D
G Protein-coupled Receptors
Wednesday, 09.11.2016 Chair: Iris Behrmann, Luxembourg 11.15 – 13.00 Bastian Schirmer, Hannover
11.15h D1 Keynote:
Control of leukocyte migration by distinct signaling complexes
Legler D.F. Kreuzlingen/Switzerland
11.45h D2 The role of 5-HT7 receptor signaling in regulation of CCR7-mediated migration of
dendritic cells and its association with inflammatory diseases of the gastrointestinal tract Holst K., Guseva D., Schindler S., Sixt M., Braun A., Moschovakis L., Chopra H., Pabst O., Förster R., Ponimaskin E. Hannover
12.00h D3 Targeting endothelial barriers in sepsis via sphingosine 1-phosphate (S1P) and
S1P receptors Jeyapaul J., Gräler MH. Jena
12.15h D4 Sphingosine-1-phosphate (S1P) in genotoxic stress-induced protection against
septic shock Weigel C., Schröder N.H., Gräler M.H. Jena
12.30h D5 GIRK channels as ultra-sensitive sensors for GPCR activation in a fluorescence-
based plate format and its application for opioid research Günther T., Dasgupta P., Mann A., Schulz S. Jena
12.45h D6 Presentation by Sony Europe Ltd:
The next level of flow cytometry: Sony spectral analyzers Weissmann S., Piedavent-Salomon M., Dessing M. Braunschweig
13.00h Lunch Break / Sponsors' Exhibition
Workshop Program
13
Workshop E
Receptor and Redox Signaling
Wednesday, 09.11.2016 Chair: Karlheinz Friedrich, Jena 14.00 – 15.30 Guido Posern, Halle/Saale
14.00h E1 Keynote:
Integrating growth factor receptor and redox signaling
Dansen T.B. Utrecht/The Netherlands
14.30h E2 Distinct functional roles of AKT isoforms for migration and metastasis of breast
cancer cells Grottke A., Ewald F., Lange T., Nörz D., Herzberger C., Bach J., Grabinski N., Gräser L., Höppner F., Nashan B., Schumacher U., Jücker M. Hamburg
14.45h E3 BMP2 internalization and transfer to neighboring cells
Alborzinia H., Hortschansky P., Wölfl S. Heidelberg
15.00h E4 Crosstalk between different family members: IL27 recapitulates IFN gamma
responses in HCC cells, but is inhibited by IL6-type cytokines Rolvering C., Zimmer A.D., Kozar I., Hermanns H.M., Letellier E., Vallar L., Nazarov P.V., Nicot N., Ginolhac A., Haan S., Behrmannn I., Haan C. Belvaux/Luxembourg
15.15h E5 Specific but antibody-free absolute quantification of signaling events in cancer
patient tissues using ERLIC and targeted LC-MS Loroch S., Di Conza G., Sickmann A., Mazzone M., Zahedi R.P. Dortmund
15.30h Coffee Break / Sponsors' Exhibition
20th Joint Meeting Signal Transduction
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Workshop F
Immune Cell Signaling
Wednesday, 09.11.2016 Chair: Michael Kracht, Gießen 16.00 – 17.45 Burkhart Schraven, Magdeburg
16.00h F1 Keynote:
How effector T cell invade the central nervous system
Odoardi F. Göttingen
16.30h F2 Microenvironmental interactions between endothelial and lymphoma cells - a role
for the canonical WNT pathway in Hodgkin lymphoma Linke F., Harenberg M., von Bonin F., Arlt A., Jensen O., Klapper W., Nietert M., Beissbarth T., Bryja V., Trümper L., Wilting J., Kube D. Göttingen
16.45h F3 A Cys476Ala Lck mutation alters Lck activity and TCR signaling
Kritikos A., Kästle M., Schraven B., Simeoni L. Magdeburg
17.00h F4 NFATc1 controls the metabolism and effector function of CD8+ T cells
Klein-Hessling S., Muhammad K., Pusch T., Klein M., Flöter J., Schulze A., Müller N., Bopp T., Avots A., Serfling E. Würzburg
17.15h F5 Intracellular role of S100A8/S100A9 during cellular dynamics of phagocytes
Russo A., Wolf M., Roth J., Vogl T. Münster
17.30h F6 Presentation by Proteintech Europe Ltd:
Tips for selecting the right antibody Wagner S., Li J. Manchester/UK
17:45h Coffee Break / Sponsors' Exhibition / Poster Viewing
Workshop Program
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18:15h Poster Presentation I "one minute – one transparency" Posters PA1 – PD3 Chair: Marcus Lettau, Kiel
19:00h Poster Discussion / Sponsors' Exhibition
20:00h Dinner
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Workshop B
Focus: Molecules in Motion
Thursday, 10.11.2016 Chair: Klaudia Giehl, Gießen 08.30 – 10.30 Detlef Neumann, Hannover
08.30h B1 Keynote:
Antigens on the Move – The INs and OUTs in adaptive immunity and viral immune evasion Tampé R. Frankfurt
09.00h B2 Keynote:
Crowding at the ribosomal tunnel exit: when SRP meets the ribosome Sinning I. Heidelberg
09.30h B3 The Wnt receptor PTK7 is dynamically localized in migrating neural crest cells
Berger H., Grund A., Jacob R., Borchers A. Marburg
09.45h B4 Meprin Metalloproteases generate biologically active soluble Interleukin-6
receptor to induce trans-signaling
Arnold P., Boll I., Schumacher N., Schmidt F., Wichert R., Schneppenheim J., Lokau J., Rabe B., Scheller J., Garbers C., Rose-John S., Becker-Pauly C. Kiel
10.00h B5 An optogenetic toolbox for subcellular protein recruitment
Mühlhäuser W.W.D., Emig R., Weber W., Radziwill G. Freiburg
10.15h B6 Presentation by Wako Chemicals GmbH:
A novel affinity purification method to isolate intact exosomes
Funakoshi T., Roeckel D. Neuss
10:30h Coffee Break / Sponsors' Exhibition
Workshop Program
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Workshop G
Signaling in Infection and Inflammation
Thursday, 10.11.2016 Chair: Arnd Kieser, München 11.00 – 13.00 Norbert Reiling, Borstel
11.00h G1 Keynote:
Killing me softly: How cytotoxic T cells kill virus-infected cells in vivo
Förster, R. Hannover
11.30h G2 The murine cytomegalovirus protein M35 is a novel negative regulator of the
type I interferon response Chan B., Magalhaes V.G., Brinkmann M.M. Braunschweig
11.45h G3 Determining the impact of intrinsic immune factors on Cytomegalovirus gene
expression kinetics Rand U., Kubsch F.T., Dag F., Kasmapour B., Cicin-Sain L. Braunschweig
12.00h G4 The Wnt ligand Wnt6 promotes the survival of Mycobacterium tuberculosis in
macrophages by modulating host lipid metabolism
Brandenburg J., Schwudke D., Marwitz S., Goldmann T., Kispert A., Leitges M., Reiling N. Borstel
12.15h G5 Mutual regulation of protein kinase R and suppressor of cytokine signalling 3
Klepsch O., Gross V., Schaper F., Dittrich A. Magdeburg
12.30h G6 Signals and posttranslational modifications regulating the decapping step in
mRNA degradation pathways
Tenekeci U., Poppe M., Beuerlein K., Buro C., Kettner-Buhrow D., Xu M., Chen Z.J., Schmitz M.L., Kracht M. Gießen
12.45h G7 Presentation by New England Biolabs GmbH / Cell Signaling Technology:
PTMScan multiplex analysis of post-translational modifications using immunoprecipitation and subsequent mass spectrometry: expanded coverage of the serine and threonine kinome, and a look into MethylScan applications Textor B. Frankfurt
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13:00h Lunch Break / Sponsors' Exhibition
14:00h STS General Assembly / Sponsors' Exhibition for non-members
15:30h Coffee Break / Sponsors' Exhibition
STS/CCS Honorary Medal Award Ceremony
Thursday, 10.11.2016 Chair: Klaudia Giehl, Gießen 16.00 – 17.45
16.00 Laudatio: Klaus Pfeffer Düsseldorf
16.30 Honorary Award Lecture:
Fire and water are good servants but bad masters
Tak Wah Mak
Toronto/Canada
17.45h Coffee Break / Sponsors' Exhibition / Poster Viewing
18.15h Poster Presentation II "one minute – one transparency" Posters PE1 – PL8 Chair: Silja Wessler, Salzburg/Austria
19.00h Poster Discussion / Sponsors' Exhibition
20.00h Dinner, Party, Crosstalk Raffle
Workshop Program
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Workshop C
Focus: Organelles in Motion
Friday, 11.11.2016 Chair: Jan Faix, Hannover 09.00 – 10.30 Katharina Hieke-Kubatzky, Heidelberg
09.00h C1 Keynote:
Interferon-inducible guanylate binding proteins (GBPs) in immune defense
Pfeffer K. Düsseldorf
09.30h C2 A complex containing neuroplastin and plasma membrane Ca2+ ATPases (PMCA)
controls calcium homeostasis in T and B cells Korthals M., Herrera-Molina R., Smalla K.H., Langnaese K., Kähne T., Klemmer P., Handschuh J., Lehmann A.C., Gundelfinger E.D., Montag D., Thomas U., Fischer K.D. Magdeburg
09.45h C3 Secretory granules in human T-cell populations: Analysis of co-localization and
differential mobilization of cytotoxic effector proteins Lettau M., Armbrust F., Poch T., Drews L., Janssen O. Kiel
10.00h C4 Effect of the hypoxia-activated prodrug TH-302 on Ewing sarcoma cells in vitro
Kühne M., Marx C., Becker S., Voigt A., Spänkuch B., Beck J.F., Sonnemann J. Jena
10.15h C5 Actin and tubulin-based protrusions guide cancer cell extracellular vesicles to
supporting bystander cell in 3D co-culture and in situ
Hansen H.P., Dams M., Barnert S., Schubert R., Dürkop H., Hallek M., Pogge von Strandmann E. Köln
10.30h Coffee Break / Sponsors' Exhibition
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Workshop H
Differentiation, Stress and Death
Friday, 11.11.2016 Chair: Ingo Schmitz, Braunschweig 11.00 – 13.00 Sebastian Wesselborg, Düsseldorf
11.00h H1 Keynote:
The p53 family and its role in stress and death
Moll U. New York/USA
11.30h H2 Screening for modulators of c-FLIP activity
Schwarz L., Wink J., Bilitewski U., Schmitz I. Braunschweig
11.45h H3 Novel insights into the complexity of p66Shc activation
Khalid S., Haller M., Kremser L., Fresser F., Hermann M., Guenther J., Drasche A., Leitges M.L, Giorgio M., Baier G., Lindner H., Troppmair J. Innsbruck/Austria
12.00h H4 Interleukin-6 influences stress-signalling by reducing the expression of the
mTOR-Inhibitor REDD1 in a STAT3-dependent manner Pinno J., Bongartz H., Klepsch O., Wundrack N., Poli V., Schaper F., Dittrich A. Magdeburg
12.15h H5 Optogenetic clustering of CNK1 reveals mechanistical insights in RAF and AKT
signaling controlling cell fate decisions Fischer A., Warscheid B., Weber W., Radziwill G. Freiburg
12.30h H6 The role of MRTF transcription factors in 3D mammary acini formation
Posern G., Seifert A. Halle/Saale
12.45h H7 Altered stem-cell characteristics via the induction of three-dimensional
differentiation of human pancreatic ductal adenocarcinoma (PDAC) A818-6 cells Tawfik D., Zaccagnino A., Szczepanowski M., Klapper W., Kalthoff H., Trauzold A. Kiel
13.00h Lunch / Poster and Science Awards
15.00h End of Meeting
Poster Presentation I
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Poster Presentation I
PA1 - PD3
Wednesday, 09.11.2016, 18.15 – 19.00h
Chair: Marcus Lettau, Kiel
PA1 Bone morphogenetic protein 4 affects invasive tumor cell subpopulation Mihajlovic J., Fischer M., Hochhaus A., Clement J.H. Jena
PA2 Interaction of human mesenchymal stem cells with ovarian cancer cells results in protein and gene expression variation Yang Y.Y., Otte A., Hass R. Shanghai/China
PA3 Direct and indirect interaction of MSC with tumor cells alter mutual functionalities Melzer C., Yang YY., Hass R. Hannover
PA4 Different effects of Rac1 and Rac1b in lung adenocarcinoma cells Seiz J.R., Lohfink D., Scharlibbe L., Heipel M., Giehl K., Menke A. Gießen
PA5 Differential localization and activity of the GTPase isoforms Rac1 and Rac1b Klinke J., Heipel M., Sauer M., Menke A., Giehl K. Gießen
PA6 Reverse engineering signalling networks from perturbation data Dorel M., Klinger B., Sieber A., Blüthgen N. Berlin
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PB1 Inhibition of galectin-3 by plant-derived polysaccharides and glycoproteins impairs metastasis-associated adhesion of pancreatic ductal adenocarcinoma cells to liver endothelium
Helm O., Rätsch T., Heise E.M., Classen B., Sebens S. Kiel
PB2 D120 and K152 within the PH domain of T cell adapter SKAP55 regulate plasma membrane targeting of SKAP55 and LFA-1 affinity modulation in human T lymphocytes Witte A., Meineke B., Sticht J., Kuropka B., Mueller A.J., Freund C., Schraven B., Kliche S. Magdeburg
PB3 A novel family of fluorescent hypoxia sensors reveal strong heterogeneity in tumor hypoxia at the cellular level
Erapaneedi R., Belousov V.V., Schäfers M., Kiefer F. Würzburg
PB4 The posttranslational modification of small GTPases in the interaction with Galectin-8 Meinohl C., Wirth S., Giehl K. Göttingen
PB5 Interrelation of Gab1 and SHP2 in pathogenic Jak2-V617F-induced MAPK signalling Bongartz H., Hessenkemper W., Mandel K., Feller S.M., Schaper F. Magdeburg
PB6 Crosstalks of the PTPIP51 interactome revealed by the novel small molecule LDC3 in Her2 breast cancer cells Dietel E., Wimmer M., Brobeil A. Gießen
PB7 Intercellular signaling via cell surface proteins – stimulus-release of the extracellular nucleotidases, CD73 and Gce1, as GPI-anchored protein / phospholipid complex in rat serum Müller G., Herling A., Tschöp M. Garching-Hochbrück
Poster Presentation I
23
PC1 Differential mobilization of individual cytotoxic effector lysosomes in human
T cells
Armbrust F., Lettau M., Janssen O.
Kiel
PC2 The mycotoxin Phomoxanthone A damages the inner mitochondrial membrane
Böhler P., Stuhldreier F., Schlütermann D., Berleth N., Wallot-Hieke N., Wu W., Frank M., Niemann H., Luyten T., Parys J., Weidtkamp-Peters S., Anand R., A.K. Kondadi A.K., Reichert A., van der Bliek, A.M., Proksch, P., Wesselborg S., Stork B. Düsseldorf
PC3 Characterization of secretory effector vesicles in leukemic T-cell populations Poch T., Lettau M., Janssen O. Kiel
PC4 Initial comparison of secretory effector vesicles from activated human CD4+ and CD8+ T cells Drews L., Lettau M., Janssen O. Kiel
PC5 Comparison of metoprolol and bisoprolol concentrations in simultaneous cerebrospinal fluid and blood serum samples
Sigaroudi A., Kinzig M., Wahl O., Schroeter M., Stelzer C., Fuhr U., Holzgrabe U., Sörgel F. Zurich/Switzerland
PD1 Live and let die: the two faces of Histamine H4-receptor in experimentally induced colitis in mice Schirmer B., Wunschel E., Neumann D. Hannover
PD2 Dynamic heterodimerization of serotonin receptors 5-HT1A and 5-HT7 regulates receptor’s functions Ponimaskin E., Zeug A., Prasad S., Renner U. Hannover
PD3 Exploring the cardio-protective potential of Pasteurella multocida toxin Chauhan C., Hildebrand D., Baur L., Rohde D., Kubatzky K.F. Heidelberg
20th Joint Meeting Signal Transduction
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Poster Presentation II
PE1 - PL8
Thursday, 10.11.2016, 18.15 – 19.00h
Chair: Silja Wessler, Salzburg/Austria
PE1 Synthetic deletion of the Interleukin 23 receptor (IL-23R) stalk region induces ligand-independent, autonomous receptor activation via IL-23R homodimerization Ackfeld T., Hummel T.M., Schönberg M., Scheller J., Floss D.M. Düsseldorf
PE2 A new identified thiol-switch regulates Zap70 stability and activity Thurm C., Simeoni L. Magdeburg
PE3 Membrane-bound Hyper IL-6 – A new designer protein to study IL-6 signaling
Lamertz L., Baran P., Floss D.M., Scheller J. Düsseldorf
PE4 A new biochemical method for rapid inducible Ras inactivation
Vasjari L., Bresan S., Rubio I. Jena
PE5 Expression and signal transduction of chemokine receptors on human neoplastic T cells Dang T.M., Lettau M., Janssen O. Kiel
Poster Presentation II
25
PF1 Tumor cell released Galectin-3 reduces proliferation of γδ T cells
Gonnermann D., Oberg H.-H., Kabelitz D., Wesch D. Kiel
PF2 Role of NBS1-dependent DNA damage response in microglial cell function in neurodegeneration
Schneble N., Wang ZQ. Jena
PF3 Role of the atypical IκB proteins in macrophage activation
Lorscheid S., Kramer D., Schulze-Osthoff K. Tübingen
PF4 Down-regulation of the sphingosine-1-phosphate receptor-2 by EBV LMP1 promotes constitutive Akt signalling and the aberrant expression of BATF in diffuse large B cell lymphoma Vockerodt M., Vrzalikova K., Ibrahim M., Nagy E., Schrader A., Kube D., Wei W., Woodman C., Murray P. Göttingen
PF5 Functional roles of atypical IkappaB family members in macrophages Matthies A., Schmitz I. Braunschweig
PF6 Lck biosensors and FLIM/FRET-measurements in Jurkat T-cells suggest a three step model for TCR-mediated signaling
Kästle M., Reddycherla A., Philipsen L., Hartig R., Kritikos A., Müller A., Simeoni L., Schraven B. Magdeburg
PF7 The role of mitochondria and the mitochondrial protein TCAIM in controlling regulatory T cell function Iwert C., Schumann J., Weigel D., Schliesser U., Sawitzki B. Berlin
PF8 In follicular regulatory T cells NFAT2 is essential for homing to germinal centers Koenig A., Vaeth M., Müller G., Stauss D., Dietz L., Klein-Hessling S., Serfling E., Lipp M., Berberich I., Berberich-Siebelt F. Würzburg
20th Joint Meeting Signal Transduction
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PG1 A regulatory role of the alarmin S100A8/S100A9 for the differentiation and activation of myeloid-derived suppressor cells (MDSC) Kuhlencord M., Zenker S., Roth J., Vogl T. Münster
PG2 The STING-mediated innate immune response to cytosolic DNA is inhibited by the m152 protein of murine cytomegalovirus Stempel M., Chan B., Lisnic V.J., Jonjic S., Brinkmann M.M. Braunschweig
PG3 Dual role of the effector protein ExoY of Pseudomonas aeruginosa in infection Schirmer B., Kloth C., Rajendraprasad G., Tsiavaliaris G., Seifert R. Hannover
PJ1 Effects of different ras inhibitors on growth properties of murine model systems of ras transformation Mixdorf T., Steven A., Stoppe S., Seliger B. Halle/Saale
PK1 Venom toxins affect intracellular signaling in primary sensory neurons - A High Content Screening microscopy approach - van Cann M., Isensee J., Berkut A.A., Kuzmenkov A.I., Vassilveski A.A., Hucho T. Köln
PL1 Laser ablation inductively-coupled plasma mass spectrometry (LA-ICP-MS) in experimental and clinical Wilson’s disease
Uerlings R., Weiskirchen R. Aachen
PL2 μ-Opioid receptor mobility in the plasma membrane is diversely affected by biased ligands
Melkes B., Hejnova L., Moravcova R., Novotny J. Prague/Czech Republic
PL3 The role of signal transducer and activator of transcription 3 (STAT3) in the rat pineal gland Moravcova S., Melkes B., Novotny J., Bendova Z. Prague/Czech Republic
Poster Presentation II
27
PL4 TRH receptor mobility in the plasma membrane is affected by its interaction with its cognate signaling molecules and by cholesterol depletion Moravcova R., Melkes B., Novotny J. Prague/Czech Republic
PL5 The protective effect of high doses of morphine on viability and oxidative state of rat cardiomyoblasts H9c2 Skrabalova J., Karlovska I., Hejnova L., Novotny J. Prague/Czech Republic
PL6 Leukemia and lymphoma cells are remarkably more susceptible to intrinsic apoptosis induced by the mycotoxin Viriditoxin than untransformed hematopoetic stem cells
Stuhldreier F., Liu Y., Jäger P., Geyh S., Berleth N., Böhler P., Schlütermann D., Wallot-Hieke N., Wu W., Proksch P., Haas R., Wesselborg S., Stork B. Düsseldorf
PL7 Communication between bladder cancer cells and stromal fibroblasts: Tumor-cell-derived interleukin-1 alpha regulates expression of malignancy-related cytokines/chemokines in fibroblasts via NF-kB Singh R., Motha V., Grimm S., Berndt A., Friedrich KH. Jena
PL8 Prostate Specific-Membrane Antigen (PSMA) assembles a macromolecular complex regulating growth and survival of prostate cancer cells in vitro and correlating with progression in vivo Perico M.E., Grasso S., Brunelli M., Martignoni G., Munari E., Moiso E., Fracasso G., Cestari T., Naim H.Y., Bronte V., Colombatti M., Ramarli D. Verona/Italy
Addresses of Sponsors
28
Sponsors of the 20th Joint Meeting Signal Transduction
Acris Antibodies GmbH
Schillerstr. 5
32052 Herford
Phone: +49 5221 34606 0
Fax: +49 5221 34606 11
Internet: www.acris-antibodies.com
Adipogen AG
Schützenstrasse 12
CH-4410 Liestal, Switzerland
Phone: +41-61-926-60-40
Fax: +41-61-926-60-49
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Berthold Technologies GmbH & Co KG
Calmbacher Straße 22
75323 Bad Wildbad
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Internet: www.berthold.com
Biomol GmbH
Waidmannstr. 35
22769 Hamburg
Phone: +49 40 85 32 60 0
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Internet: www.biomol.de
BioVendor GmbH
Im Neuenheimer Feld 583
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Biozol Diagnostica Vertrieb GmbH
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Cisbio Bioassays
Parc Marcel Boiteux
30200 Codolet, France
Phone: +33 4 66 79 67 05
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Internet: www.htrf.com
Eurogentec S.A.
Rue Bois Saint-Jean 5
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Gilson International Deutschland
Hoenbergstraße 6
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HiSS Diagnostics GmbH
Güterhallenstr. 3
79106 Freiburg
Phone: +49 761 389 49 0
Fax: +49 761 389 49 20
Internet: www.hiss-dx.de
LI-COR Biosciences GmbH
Siemensstr. 25a
61352 Bad Homburg, Germany
Phone: +49 61 72 17 17 7 71
Fax: +49 61 72 17 17 7 99
Internet: www.licor.com
MoBiTec GmbH
Lotzestraße 22a
37083 Göttingen
Phone: +49 551 70722 0
Fax: +49 551 70722 22
Internet: www.mobitec.com
20th Joint Meeting Signal Transduction
29
Molecular Devices (Germany) GmbH
Bismarckring 39
88400 Biberach an der Riss
Phone: +49 800 665 32860
Fax: +49 800 665 32840
Internet: www.moleculardevices.com
New England Biolabs GmbH / Cell
Signaling Technology Inc.
Brüningstr. 50 – Geb. B852
65926 Frankfurt
Phone: +49 69 305 2314 0
Fax: +49 69 305 2314 9
Internet: www.neb-online.de
OLS OMNI Life Science GmbH & Co. KG
Karl-Ferdinand-Braun-Str. 2
28359 Bremen
Phone: +49 421 27 61 69-0
Fax: +49 421 27 61 69-19
Internet: www.ols-bio.de
PerkinElmer LAS GmbH
Ferdinand-Porsche-Ring 17
63110 Rodgau-Jügesheim
Phone: +49 6106 610 0
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Internet: www.perkinelmer.de
PromoCell GmbH
Sickingenstr. 63/65
69126 Heidelberg
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Internet: www.promocell.com
Proteintech Europe Ltd
4th Floor, 196 Deansgate
Manchester M3 3WF
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Sarstedt AG & Co
Sarstedtstr. 1
51588 Nümbrecht
Phone: +49 2293 305 0
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Internet: www.sarstedt.com
SERVA Electrophoresis GmbH
Carl-Benz-Str. 7
69115 Heidelberg
Phone: +49 6221 13840 0
Fax: +49 6221 13840 10
Internet: www.serva.de
Sony Europe Ltd
Kemperplatz 1
10785 Berlin
Phone: +49 30 41955 1000
Fax: +49 30 41955 2000
Internet: www.sony.de
tebu-bio GmbH
Berliner Str. 255
63607 Offenbach
Phone: +49 69 80 10 13 0
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Internet: www.tebu-bio.com
Thermo Fisher Scientific Inc.
Adenauerallee 113
53113 Bonn
Phone: +49 228 912565 0
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Internet: www.thermoscientific.com
Wako Chemicals GmbH
Fuggerstr. 12
D - 41468 Neuss
Phone: +49 2131 311-0
Fax: +49 2131 311-110
Internet: www.wako-chemicals.de
Register of Presentations listed by Presenting Authors
30
Register of Presentations
Armbrust, F. PC1
Becker-Pauly, C. B4
Behrmannn, I. E4
Bongartz, H. PB5
Böhler, P. PC2
Borchers, A. B3
Brandenburg, J. G4
Brinkmann, M.M. G2
Chauhan, C. PD3
Dang, T.M. PE5
Dansen, T.B. E1
Dietel, E. PB6
Dorel, M. PA6
Erapaneedi, R. PB3
Faix, J. A1
Funakoshi, T. B6
Floss, D.M. PE1
Förster, R. G1
Gonnermann, D. PF1
Grosse, R. A2
Günther, T. D5
Hansen, H.P. C5
Helm, O. PB1
Holst, K. D2
Iwert, C. PF7
Janssen, O. PC3, PC4
Jeyapaul, J. D3
Jücker, M. E2
Kästle, M. PF6
Karlovska, I. PL5
Klepsch, O. G5
Kliche, S. PB2
Klinke, J. PA5
Koenig, A. PF8
Korthals, M. C2
Kracht, M. G6
Kritikos, A. F3
Kube, D. F2
Kühne, M. C4
Lamertz, L. PE3
Lammert, A. A3
Legler, D.F. D1
Lettau, M. C3
Loroch, S. E5
Lorscheid, S. PF3
Mak, T.W. HAL*
Matthies, A. PF5
Meinohl, C. PB4
Melkes, B. PL2
Melzer, C. PA3
Mihajlovic, J. PA1
Mixdorf, T. PJ1
Moll, U. H1
Moravcova, R. PL4
Moravcova, S. PL3
Mühlhäuser, W. B5
Müller, G. PB7
Neumann, D. PD1
Odoardi, F. F1
Pfeffer, K. C1
Ponimaskin, E. PD2
Posern, G. H6
Radziwill, G. H5
Rand, U. G3
Sachse, C. A4
Schaper, F. H4
Schirmer, B. PG3
Schneble, N. PF2
Schwarz, L. H2
Seiz, J.R. PA4
Serfling, E. F4
20th Joint Meeting Signal Transduction
31
Sigaroudi, A. PC5
Singh, R. PL7
Sinning, I. B2
Stempel, M. PG2
Stuhldreier, F. PL6
Tampé, R. B1
Tawfik, D. H7
Textor, B. G7
Thurm, C. PE2
Troppmair, J. H3
Ungefroren, H. A5
van Cann, M. PK1
Vasjari, L. PE4
Vockerodt, M. PF4
Vogl, T. F5, PG1
Wagner, S. F6
Weigel, C. D4
Weiskirchen, R. PL1
Weissmann, S. D6
Wölfl, S. E3
Yang, Y.Y. PA2
HAL* Honorary Award Lecture