silver scurf, black scurf and dry rot · silver scurf, black scurf and dry rot ragnhild nærstad...
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Silver scurf, black scurf
and dry rot
Ragnhild Nærstad
Norwegian Institute for Agricultural and
Environmental Research
Bioforsk
Plant Health and Plant Protection Division
.
14 000 ha -> 350 000 tons = 25 t/ha (10 – 70 t/ha)
3000 growers, 1 – 80 ha of potatoes per grower
Silver scurf Helminthosporium solani
Silver scurf
• Potato is the only known host plant
• Survives as a saprophyte in soil, and multiplies
on senescent plants of many species
• Tubes can become infected in the growing
season, at harvest or during storage
• Needs humid conditions to infect, the infection
speed increases with temperature
• Under optimal conditions the fungus needs
9 hours to penetrate into the cells
Silver scurf
• Infected cells die, loose their pigments and
become filled with air –> silvery lesions
• Increased the water loss in infected tissue ->
shrinking
• Inoculum sources: Seed tubers (and soil)
• PCR results from 2008 - 2009: a lot of
inoculum in seed tubers and low levels in
soil
Control measures – Silver scurf
• Crop rotation + avoid cereals as the last crop before
potatoes
• Shorter growing seasons (presprout seed tubers)+
shorten the period between haulm killing and harvest
• Suppressive soils
• Low plant density
• Dry curing
• Cold storage and no condense
• Seed treatment: Fungazil (imazalil), Maxim
(fludioxonil), Emesto (penflufen), Emesto silver
(penflufen + protioconazol), Serenade (Basillus subtilis)
• In furrow fungicide application: Amistar (azoxystrobin)
Black scurf
Rhizoctonia solani
Sclerotia and
hypha
Black scurf Rhizoctonia solani
(Thanatephorus cucumeris)
• Sunken dark brown lesions on
sprouts, stems, stolons and roots
• Infections in stolons early in the
season -> tuber abortion, new
tubers is developed higher up ->
more small tubers and more green
tubers
Control measures – Black scurf
• Crop rotation, several years without potatoes
• Suppressive soils
• Shorter growing seasons + shorten the period between
haulm killing and harvest -> less sclerotia on tubers
• Dry curing
• Cold storage and no condense
• Seed treatment: Rizolex (tolclofosmethyl), Monceren
(pencycuron), Maxim (fludioxonil), Emesto
(penflufen), Emesto silver (penflufen + protioconazol)
Silver scurf
Silver scurf
Black dot
Plug test - incubation test
- distinguish between different types of scurfs
- visualize latent infection
Occurrence of skin blemish diseases in Norway
after 4 - 6 month of storage, 247 potato lots: Nærstad R, Dees MW, Le VH, Holgado R, Hermansen A
• Silver scurf (Helminthosporium solani) 11-13% (100%)
• Black dot (Colletotrichum coccodes) (80%)
• Common scab (Streptomyces spp.) 6-7% (50-70%)
• Powdery scab (Spongospora subterranean) 0.7-2% (70%)
• Skin spot (Polyscytalum pustulans) 0.2-1.7% (80%)
• Black scurf (Rhizoctonia solani) 0.8% (80%)
Root lesion nematodes
Paratylenchchus spp. in
60% of scabby lesions
Decision making in potato skin blemish
diseases control using real-time diagnostic
methods; Norwegian results from 2009 and
2010
R. Nærstad, A. Hermansen, V.H. Le,
G. Lund, M. Skogen, M.B. Brurberg
Sampling
• 40 locations each year in 2009 and 2010 + questionaries
• From each location:
– Samples of soil and seed tubers at planting
– Samples of soil in August
– Samples of progeny tubers at harvest
• Assessed for the presence of pathogens by real-time PCR
• Tubers were also assed for the presence of pathogens by plug tests
• Pathogens:
• Black dot (Colletotrichum coccodes)
• Skin spot (Polyscytalum pustulans)
• Common scab (Streptomyces spp.)
• Silver scurf (Helminthosporium solani)
• Powdery scab (Spongospora subterranean)
• Black scurf (Rhizoctonia solani)
Real-time PCR protocols
• SCRI protocols:
• Common scab (Cullen and Lees, 2007)
• Powdery scab (van de Graaf et al., 2003)
• Silver scurf (Cullen et al., 2001)
• Black scurf (Lees et al., 2002)
• Black dot (Cullen et al. 2002)
• Fungal pathogens based on ITS regions
• Streptomyces based on nec gene
18S gen 5.8S gen 26S gen
ITS Ribosomale RNA gener:
DNA-analysis
• Soil samples (SCRI protocol)
• Tuber samples
– Peel homogenized in Homex (SCRI protocol)
– Extraction robot (Molgene)
Example of amplification plot
Silver scurf Helminthosporium solani
543210
90
80
70
60
50
40
30
20
10
0
PCR-Silver scurf-progeny tubers
%S
-Silv
er
scu
rf-p
rog
en
y t
ub
ers
2009
2010
year
Scatterplot of %S-Silver scurf-progeny vs PCR-Silver scurf-progeny
• Good correlation between plug-test and PCR results
• High level of inoculum in seed tubers
• Low level of inoculum in soil
• Low correlation between inoculum in seed tubers and progeny tubers
543210
5,6
5,2
4,8
4,4
4,0
PCR-Silver scurf-progeny tubers
PC
R-S
ilve
r scu
rf-s
ee
d t
ub
ers
2009
2010
year
Scatterplot of PCR-Silver scurf-seed tu vs PCR-Silver scurf-progeny
5,65,24,84,44,0
100
80
60
40
20
0
PCR-Silver scurf-seed tubers
%S
-Silv
er
scu
rf-s
ee
d t
ub
ers
Scatterplot of %S-Silver scurf-seed tub vs PCR-Silver scurf-seed tu
Black scurf Rhizoctonia solani
6543210
2,5
2,0
1,5
1,0
0,5
0,0
PCR-black scurf- seed tubers
Scle
roti
a-
Se
ed
tu
be
rs
Scatterplot of Sclerotia- Seed tubers vs PCR-black scurf- seed tubers
• Good correlation between: sclerotia plug-test plug-test PCR results
• Low level of inoculum in soil in spring, a slight increase in the autumn, but did not correlate with the black scurf in the progeny
• Low correlation between inoculum in seed and progeny tubers
6543210
50
40
30
20
10
0
PCR-black scurf- seed tubers
%R
-Bla
ck s
cu
rf-s
ee
d t
ub
ers
Scatterplot of %R-Black scurf-seed tube vs PCR-black scurf- seed tu
9080706050403020100
5
4
3
2
1
0
%R-black scurf-progeny tubers
Scle
roti
a-p
rog
en
y t
ub
ers
2009
2010
Year
Scatterplot of Sclerotia-progeny tubers vs %R-black scurf-progeny t
Conclusion from quantitative PCR
diagnostics of soil and tubers
• Skin spot, powdery scab and black dot:
disease increased with inoculums on the seed
tubers.
• For black dot and powdery scab it also
increased with soil inoculums
• Common scab, silver surf and black scurf:
other factors than inoculums level were more
important for disease incidence in the progeny
tubers
Harvest, curing and storage strategies
3 trials
• 0, 11 and 22 days from haulm killing to
harvest
• 4 harvest dates
• Curing: 70% RH and 90% RH
• Storage: Directly to 4oC and slowly to 4oC
Seed treatment at planting (6 trials/year)
0
10
20
30
40
50
60
% progeny tubers with sclerotia (2005 and 2006)
% plugs with black scurf hyfae (2007)
% plugs with silver scurf (2007)
Control
Rizolex
Maxim
• Both Maxim (fludioxonil) and Rizolex
(tolclofosmethyl) reduces black scurf in the progeny
tubers (sclerotia og hypha)
• Maxim reduces silver scurf in the progeny
0
10
20
30
40
50
60
% plugs with silver scurf % plugs with black scurf hyfae
% plugs with skin spot
Effect of humidity during curing and Amistar in furrow
Control Humid curing
Control Dry curing
Amistar Humid curing
Amistar Dry curing
• All treatments reduced the
amount of R. solani sclerotia
and hypha on the progeny
tubers
• Amistar and Emesto also
reduced the frequency of
silver scurf latent infections
(plug test)
• In 2008 and 2010 there were
attack of black dot at one
location and in these trials
there were less black dot in
the Amistar treatment.
0
10
20
30
40
50
60
70
80
90
100
2008 2009 2010
% reduction of black scurf hyfae
Rizolex
Emesto
Amistar
Emesto dust
Emesto silver
Prestige
-100
-80
-60
-40
-20
0
20
40
60
80
100
2008 2009 2010
% reduction of silver scurf
Rizolex
Emesto
Amistar
Emesto dust
Emesto silver
Prestige
-40
-20
0
20
40
60
80
100
2008 2010
% reduction of black dot
Rizolex
Emesto
Amistar
Emesto dust
Emesto silver
Prestige
Fusarium dry rot
• Important disease of potato affecting tubers in
storage
• Caused by several fungal species in the genus
Fusarium
• Fusarium dry rot of seed tubers can reduce crop
establishment by killing developing potato sprouts
• Crop losses can be up to 25%
Symptoms
• Originate in wounds created by mechanical damages or
desprouting during handling
• Surface lesions appears as dark depressions and wrinkled
areas of skin
• Concentric rings can be observed in case of heavy infection
• Fusarium dry rot can be recognized based on white, pink or
orange colored mycelium proliferation on the tuber surface
Photos: J. Gomez
Fusarium dry rot in Norway
P.H. Jensen, M.B. Brurberg & A. Hermansen • Main goal: Find out which
Fusarium spp. currently causes
dry rot problems in Norway
• How: Potato samples collected
from different regions in Norway
over 3 seasons (2010-2013)
– artificial wounding +
incubation
– isolation
Photo: P. H. Jensen
Results from the growing season 2010 -2011
5 % of the tubers developed rots
Fusarium
Boeremia (Phoma)
Penicillium
Mucor
Cylindrocarbon
49 % of rots were caused by Fusarium
–F. coeruleum (64%)
–F. avenaceum (31%)
–F. culmorum (4%)
–F. cerealis (1%)
–F. sambucinum (1%)
Photo: P. H. Jensen
Fusarium sambucinum Fusarium culmorum
Fusarium coeruleum Fusarium cerealis
Goal
• Develop multiplex real time PCR for
Norwegian dry rots caused by Fusarium
species and gangrene (Boremia foveata)
Monceren FS 250(Pencycuron) Inhibition of cell division, phenylureas,
FRAC Code B4
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Monceren FS250 (Pencycuron in ppm )
Colletotrichum coccodes
No08-Cc-016
No08-Cc-037
No08-Cc-057
No08-Cc-10
No08-Cc-13
No08-Cc-19
No08-Cc-42
No08-Cc-43
No08-Cc-47
No08-Cc-50
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Monceren FS250 (Pencycuron in ppm )
Helminthosporium solani
No08-Hs-059
No08-Hs-060
No08-Hs-062
No08-Hs-067
No08-Hs-020
No08-Hs-021
No08-Hs-027
No08-Hs-029
No08-Hs-035
No08-Hs-041
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Monceren FS250 (Pencycuron in ppm )
Polyscytalum pustulans
No08-Pp-034
No08-Pp-043
No08-Pp-048
No08-Pp-053
No08-Pp-001
No08-Pp-005
No08-Pp-009
No08-Pp-011
No08-Pp-012
No08-Pp-015
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Monceren FS250 (Pencycuron in ppm )
Rhizoctonia solani
No08-Rs-020
No08-Rs-025
No08-Rs-027
No08-Rs-130
No08-Rs-004
No08-Rs-011
No08-Rs-013
No08-Rs-014
No08-Rs-016
No08-Rs-019
Rizolex 50FW (Tolclofosmethyl) Lipid peroxidation, Aromatic Hydrocarbons,
FRAC Code F3
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Rizolex 50 FW(Tolclophosmethyl in ppm )
Colletotrichum coccodes
No08-Cc-016
No08-Cc-037
No08-Cc-057
No08-Cc-10
No08-Cc-13
No08-Cc-19
No08-Cc-42
No08-Cc-43
No08-Cc-47
No08-Cc-50
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Rizolex 50 FW(Tolclophosmethyl in ppm )
Helminthosporium solani
No08-Hs-059
No08-Hs-060
No08-Hs-062
No08-Hs-067
No08-Hs-020
No08-Hs-021
No08-Hs-027
No08-Hs-029
No08-Hs-035
No08-Hs-041
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Rizolex 50 FW(Tolclophosmethyl in ppm )
Polyscytalum pustulans
No08-Pp-034
No08-Pp-043
No08-Pp-048
No08-Pp-053
No08-Pp-001
No08-Pp-005
No08-Pp-009
No08-Pp-011
No08-Pp-012
No08-Pp-015
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Rizolex 50 FW(Tolclophosmethyl in ppm )
Rhizoctonia solani
No08-Rs-020
No08-Rs-025
No08-Rs-027
No08-Rs-130
No08-Rs-004
No08-Rs-011
No08-Rs-013
No08-Rs-014
No08-Rs-016
No08-Rs-019
Maxim 100 FS(fludioxonil) Inhibition of osmotic signal transduction, phenylpyrroles,
FRAC Code E2
0
20
40
60
80
100
120
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Maxim 100 FS (fludioxonil in ppm )
Colletotrichum coccodes
No08-Cc-016
No08-Cc-037
No08-Cc-057
No08-Cc-10
No08-Cc-13
No08-Cc-19
No08-Cc-42
No08-Cc-43
No08-Cc-47
No08-Cc-50
0
20
40
60
80
100
120
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Maxim 100 FS (Fludioxonil in ppm )
Helminthosporium solani
No08-Hs-059
No08-Hs-060
No08-Hs-062
No08-Hs-067
No08-Hs-020
No08-Hs-021
No08-Hs-027
No08-Hs-029
No08-Hs-035
No08-Hs-041
0
20
40
60
80
100
120
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Maxim 100 FS (Fludioxonil in ppm )
Polyscytalum pustulans
No08-Pp-034
No08-Pp-043
No08-Pp-048
No08-Pp-053
No08-Pp-001
No08-Pp-005
No08-Pp-009
No08-Pp-011
No08-Pp-012
No08-Pp-015
0
20
40
60
80
100
120
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Maxim 100 FS (Fludioxonil in ppm )
Rhizoctonia solani
No08-Rs-020
No08-Rs-025
No08-Rs-027
No08-Rs-130
No08-Rs-004
No08-Rs-011
No08-Rs-013
No08-Rs-014
No08-Rs-016
No08-Rs-019
Emesto(Penflufen) Inhibition of respiration complex II, Succinate DeHydrogenase Inhibitors,
FRAC Code C2
0
20
40
60
80
100
120
140
160
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
BYF14182 (Penflufen in ppm )
Colletotrichum coccodes
No08-Cc-016
No08-Cc-037
No08-Cc-057
No08-Cc-10
No08-Cc-13
No08-Cc-19
No08-Cc-42
No08-Cc-43
No08-Cc-47
No08-Cc-50
0
20
40
60
80
100
120
140
160
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
BYF14182 (Penflufen in ppm )
Helminthosporium solani
No08-Hs-059
No08-Hs-060
No08-Hs-062
No08-Hs-067
No08-Hs-020
No08-Hs-021
No08-Hs-027
No08-Hs-029
No08-Hs-035
No08-Hs-041
0
20
40
60
80
100
120
140
160
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
BYF14182 (Penflufen in ppm )
Polyscytalum pustulans
No08-Pp-034
No08-Pp-043
No08-Pp-048
No08-Pp-053
No08-Pp-001
No08-Pp-005
No08-Pp-009
No08-Pp-011
No08-Pp-012
No08-Pp-015
0
20
40
60
80
100
120
140
160
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
BYF14182 (Penflufen in ppm )
Rhizoctonia solani
No08-Rs-020
No08-Rs-025
No08-Rs-027
No08-Rs-130
No08-Rs-004
No08-Rs-011
No08-Rs-013
No08-Rs-014
No08-Rs-016
No08-Rs-019
Fungazil(Imazalil) Inhibition of sterol biosynthesis in membranes, DeMethylation Inhibitors,
FRAC Code G1
0
20
40
60
80
100
120
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Fungazil 100SL (Imazalil in ppm )
Colletotrichum coccodes
No08-Cc-016
No08-Cc-037
No08-Cc-057
No08-Cc-10
No08-Cc-13
No08-Cc-19
No08-Cc-42
No08-Cc-43
No08-Cc-47
No08-Cc-50
0
20
40
60
80
100
120
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Fungazil 100SL (Imazalil in ppm )
Helminthosporium solani
No08-Hs-059
No08-Hs-060
No08-Hs-062
No08-Hs-067
No08-Hs-020
No08-Hs-021
No08-Hs-027
No08-Hs-029
No08-Hs-035
No08-Hs-041
0
20
40
60
80
100
120
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Fungazil 100SL (Imazalil in ppm )
Polyscytalum pustulans
No08-Pp-034
No08-Pp-043
No08-Pp-048
No08-Pp-053
No08-Pp-001
No08-Pp-005
No08-Pp-009
No08-Pp-011
No08-Pp-012
No08-Pp-015
0
20
40
60
80
100
120
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Fungazil (Imazalil in ppm )
Rhizoctonia solani
No08-Rs-020
No08-Rs-025
No08-Rs-027
No08-Rs-130
No08-Rs-004
No08-Rs-011
No08-Rs-013
No08-Rs-014
No08-Rs-016
No08-Rs-019
Proline EC250 (Protiokonazol) Inhibition of sterol biosynthesis in membranes, DeMethylation Inhibitors,
FRAC Code G1
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Proline EC250 (Protiokonazol in ppm )
Colletotrichum coccodes
No08-Cc-016
No08-Cc-037
No08-Cc-057
No08-Cc-10
No08-Cc-13
No08-Cc-19
No08-Cc-42
No08-Cc-43
No08-Cc-47
No08-Cc-50
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Proline EC250 (Protiokonazol in ppm )
Helminthosporium solani
No08-Hs-059
No08-Hs-060
No08-Hs-062
No08-Hs-067
No08-Hs-020
No08-Hs-021
No08-Hs-027
No08-Hs-029
No08-Hs-035
No08-Hs-041
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Proline EC250 (Protiokonazol in ppm )
Polyscytalum pustulans
No08-Pp-034
No08-Pp-043
No08-Pp-048
No08-Pp-053
No08-Pp-001
No08-Pp-005
No08-Pp-009
No08-Pp-011
No08-Pp-012
No08-Pp-015
0
20
40
60
80
100
120
140
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Proline EC250 (Protiokonazol in ppm )
Rhizoctonia solani
No08-Rs-020
No08-Rs-025
No08-Rs-027
No08-Rs-130
No08-Rs-004
No08-Rs-011
No08-Rs-013
No08-Rs-014
No08-Rs-016
No08-Rs-019
Amistar (azoxystrobin) + Salicylhydroxamic acid Inhibition of respiration complex III, Quinone outside inhibitors
FRAC Code C3,
0
25
50
75
100
125
150
175
200
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Amistar (Azoxystrobin in ppm )
Colletotrichum coccodes
No08-Cc-016
No08-Cc-037
No08-Cc-057
No08-Cc-10
No08-Cc-13
No08-Cc-19
No08-Cc-42
No08-Cc-43
No08-Cc-47
No08-Cc-50
0
25
50
75
100
125
150
175
200
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Amistar (Azoxystrobin in ppm )
Helminthosporium solani
No08-Hs-059
No08-Hs-060
No08-Hs-062
No08-Hs-067
No08-Hs-020
No08-Hs-021
No08-Hs-027
No08-Hs-029
No08-Hs-035
No08-Hs-041
0
25
50
75
100
125
150
175
200
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Amistar (Azoxystrobin in ppm )
Polyscytalum pustulans
No08-Pp-034
No08-Pp-043
No08-Pp-048
No08-Pp-053
No08-Pp-001
No08-Pp-005
No08-Pp-009
No08-Pp-011
No08-Pp-012
No08-Pp-015
0
25
50
75
100
125
150
175
200
0,01 0,10 1,00 10,00 100,00
Gro
wth
in %
of
con
tro
l
Amistar (Azoxystrobin in ppm )
Rhizoctonia solani
No08-Rs-020
No08-Rs-025
No08-Rs-027
No08-Rs-130
No08-Rs-004
No08-Rs-011
No08-Rs-013
No08-Rs-014
No08-Rs-016
No08-Rs-019
Application of seed treatment in
autumn or spring ?
The control had silver scurf on 50% of the plugs
and black scurf on 12 % of the plugs =>no
significant differenses between treatments for
black scurf.
0
10
20
30
40
50
60
70
80
90
100
Autumn Spring Autumn + spring
% control of silvers curf in progeny tubers
Imazalil sulfat
Fludioxonil
Copperoxide
Penflufen