simulation results
DESCRIPTION
Simulation Results. Mutation results for MG1655 and O157:H7 E-Coli strains. Detected Translation Signals. MG1655. Detected translation signals. -1. -2. -3. Termination. Codon. Initiation Codon. -4. Average Free Energy (kcal/mol). -5. -6. -7. Shine Dalgarno. Signal. -8. - PowerPoint PPT PresentationTRANSCRIPT
Simulation Results
Mutation results for MG1655 and O157:H7 E-Coli strains
0 50 100 150 200 250 300 350 400 450 500-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Detected translation signals
Coding (translated)
Non coding (arbitrary)
Shine DalgarnoSignal
Initiation Codon
Termination Codon
MG1655Detected Translation Signals
Detected Translation Signals O157:H7
0 50 100 150 200 250 300 350 400 450 500-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Detected translation signals
Non coding (arbitrary)
Coding (translated)
Shine DalgarnoSignal
Initiation Signal
Termination Signal
Mutations• Experimental results obtained by mutating regions of the 3’ end of the
16SrRNA are compared with results obtained by incorporating these mutations in the 16SrRNA based codebook of our model.
• Jacob introduced a point mutation in the 5th position of the 16SrRNA [16]. Specifically, the 5th position in the arrangement illustrated below:
Original UAAGGAGGUGAUC Mutated UAACCUCCUGAUC
• This point mutation consisted in a change of the nucleotide C → U in the ribosome small subunit. This is equivalent to make a mutation from G → A in the complement sequence shown above. The result of this mutation was a reduction in the level of protein synthesis. Fig. 1 and Fig. 2 show how the recognition of the Shine-Dalgarno signal is affected for the Jacob mutation. It can be inferred from the plot that the levels of protein production will be reduced but not completely stopped.
MG1655
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position (101 initiation codon)
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Results with Jacob mutation
No mutaion
G to A
Partial lossdue to mutation
Fig 1
O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position (101 initiation codon)
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Results with Jacob mutation
No mutaion
G to A
Partial Loss
due to mutation
Fig 2
• Hui and De Boer did another published record of the behavior of the protein synthesis under mutations in the 3’ end of the 16SrRNA.
• In this experiment, the mutations were done in positions 4 to 8(GGAGG → CCUCC) and positions 5 to 7 (GAG → UGU).
Original UAAGGAGGUGAUC Mutated 1 UAACCUCCUGAUC Mutated 2 UAACUGUCUGAUC
• The results of both mutations were lethal for the organism in the sense that the production of proteins stopped. After introducing these two mutations, the results showed a complete loss of the SD signal. Hence, it can be inferred that the translation will never take place. This is illustrated in Fig. 3 and Fig. 4.
40 60 80 100 120 140 160-9
-8
-7
-6
-5
-4
-3
-2
-1
Position (101 initiation codon)
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Results with Hui and De Boer mutations
No mutaion
GGAGG to GUGUG
GGAGG to CCUCC
Complete lossdue to mutation
MG1655
Fig 3
40 60 80 100 120 140 160-9
-8
-7
-6
-5
-4
-3
-2
-1
Position (101 initiation codon)
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Results with Hui and De Boer mutations
No mutaion
GGAGG to GUGUG
GGAGG to CCUCC
O157:H7
Fig 4
Point Mutations
• To exploit the model further, point mutations have been introduced in all positions of the last 13 bases of the 16SrRNA molecule in order to study their influence on the process of translation.
• The obtained results are summarized in Table I by quantizing into 5 levels the influence of these mutations on each of the translation signals (SD, initiation, stop). The levels are:
– represents no influence in the recognition of the signal, represents a strong negative influence ↓ a weak influence ↑ a weak positive influence a strong positive influence.
Table I: POINT MUTATIONS IN 16SrRNA
For example, results show how a mutation in position 5 has a strong negative influence in the recognition of the SD signal, just as found in the Jacob investigation.
Remarkable Findings
• A mutation in position 8 has no influence in the detection of the translation signals, probably the reason is that the role of this nucleotide is to introduce spacing at the moment of decoding the mRNA sequence.
• A mutation at position 6 has nearly the same influence as a mutation at position 5.
• A mutation at position 9 affects the recognition of the initiation codon even if it does not affect the SD signal. This could lead to a wrong initiation of translation or a “frame shift”.
• Exactly the central part of the 13 bases (bases 4-8) which influences the SD is missing in eukaryotes. The rest of the sequence that involves AUG and stop codon recognition are still there.
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position (101 initiation codon)
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 1st position (U to A)
No mutaion
1st position (U to A)
MG1655
Mutation in the 1st Position (U to A) (SD ─ , IC ─ , SC ↓ )
1st Position
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 1st position (U to A)
No mutaion
1st position (U to A)
1st Position
Mutation in the 1st Position (U to A) (SD ─ , IC ─ , SC ↓ )
O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 1st position (U to A)
No mutaion
1st position (U to A)
MG1655
Mutation in the 1st Position (U to A) (SD ─ , IC ─ , SC ↓ )
1st Position
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 1st position (U to A)
No mutaion
1st position (U to A)
1st Position
Mutation in the 1st Position (U to A) (SD ─ , IC ─ , SC ↓ )
O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 2nd position (A to U)
No mutaion
2nd position (A to U)
MG1655
Mutation in the 2nd Position (U to A) (SD ─ , IC ─ , SC )
2nd Position
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 2nd position (U to A)
No mutaion
2nd position (A to U)
Mutation in the 2nd Position (U to A) (SD ─ , IC ─ , SC )
2nd Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 2nd position (A to U)
No mutaion
2nd position (A to U)
MG1655
Mutation in the 2nd Position (U to A) (SD ─ , IC ─ , SC )
2nd Position
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 2nd position (U to A)
No mutaion
2nd position (A to U)
Mutation in the 2nd Position (U to A) (SD ─ , IC ─ , SC )
2nd Position O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 3rd position (A to U)
No mutaion
3rd position (A to U)
MG1655
Mutation in the 3rd position (A to U) (SD ─ , IC , SC ↓ )
3rd Position
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 3rd position (A to U)
No mutaion
3rd position (A to U)
O157:H7
Mutation in the 3rd position (A to U) (SD ─ , IC , SC ↓ )
3rd Position
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 3rd position (A to U)
No mutaion
3rd position (A to U)
MG1655
Mutation in the 3rd position (A to U) (SD ─ , IC , SC ↓ )
3rd Position
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 3rd position (A to U)
No mutaion
3rd position (A to U)
O157:H7
Mutation in the 3rd position (A to U) (SD ─ , IC , SC ↓ )
3rd Position
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 4th position (G to C)
No mutaion
4th position (G to C)
MG1655
Mutation in the 4th position (G to C) (SD ↓ , IC , SC ─ )
4th Position
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 4th position (G to C)
No mutaion
4th position (G to C)
Mutation in the 4th position (G to C) (SD ↓ , IC , SC ─ )
4th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 4th position (G to C)
No mutaion
4th position (G to C)
MG1655
Mutation in the 4th position (G to C) (SD ↓ , IC , SC ─ )
4th Position
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 4th position (G to C)
No mutaion
4th position (G to C)
Mutation in the 4th position (G to C) (SD ↓ , IC , SC ─ )
4th Position O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 5th position (G to C)
No mutaion
5th position (G to C)
MG1655
Mutation in the 5th position (G to C) (SD , IC ↓ , SC ─ )
5th Position
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 5th position (G to C)
No mutaion
5th position (G to C)
Mutation in the 5th position (G to C) (SD , IC ↓ , SC ─ )
5th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 5th position (G to C)
No mutaion
5th position (G to C)
MG1655
Mutation in the 5th position (G to C) (SD , IC ↓ , SC ─ )
5th Position
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 5th position (G to C)
No mutaion
5th position (G to C)
Mutation in the 5th position (G to C) (SD , IC ↓ , SC ─ )
5th Position O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 6th position (A to U)
No mutaion
6th position (A to U)
Mutation in the 6th position (A to U) (SD , IC ↓ , SC ─ )
6th Position MG1655
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 6th position (A to U)
No mutaion
6th position (A to U)
Mutation in the 6th position (A to U) (SD , IC ↓ , SC ─ )
6th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 6th position (A to U)
No mutaion
6th position (A to U)
Mutation in the 6th position (A to U) (SD , IC ↓ , SC ─ )
6th Position MG1655
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 6th position (A to U)
No mutaion
6th position (A to U)
Mutation in the 6th position (A to U) (SD , IC ↓ , SC ─ )
6th Position O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 7th position (G to C)
No mutaion
6th position (G to C)
Mutation in the 7th position (G to C) (SD ↓ , IC ↓ , SC ─ )
7th Position MG1655
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 7th position (G to C)
No mutaion
6th position (G to C)
Mutation in the 7th position (G to C) (SD ↓ , IC ↓ , SC ─ )
7th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 7th position (G to C)
No mutaion
6th position (G to C)
Mutation in the 7th position (G to C) (SD ↓ , IC ↓ , SC ─ )
7th Position MG1655
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 7th position (G to C)
No mutaion
6th position (G to C)
Mutation in the 7th position (G to C) (SD ↓ , IC ↓ , SC ─ )
7th Position O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 8th position (G to C)
No mutaion
8th position (G to C)
Mutation in the 8th position (G to C) (SD ─ , IC ─ , SC ─ )
8th Position MG1655
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 8th position (G to C)
No mutaion
8th position (G to C)
Mutation in the 8th position (G to C) (SD ─ , IC ─ , SC ─ )
8th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 8th position (G to C)
No mutaion
8th position (G to C)
Mutation in the 8th position (G to C) (SD ─ , IC ─ , SC ─ )
8th Position MG1655
Mutation in the 8th position (G to C) (SD ─ , IC ─ , SC ─ )
8th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 8th position (G to C)
No mutaion
8th position (G to C)
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 9th position (U to A)
No mutaion
9th position (U to A)
Mutation in the 9th position (U to A) (SD ─ , IC , SC ↓ )
9th Position MG1655
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 9th position (U to A)
No mutaion
9th position (U to A)
Mutation in the 9th position (U to A) (SD ─ , IC , SC ↓ )
9th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 9th position (U to A)
No mutaion
9th position (U to A)
Mutation in the 9th position (U to A) (SD ─ , IC , SC ↓ )
9th Position MG1655
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 9th position (U to A)
No mutaion
9th position (U to A)
Mutation in the 9th position (U to A) (SD ─ , IC , SC ↓ )
9th Position O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 10th position (G to C)
No mutaion
10th position (G to C)
Mutation in the 10th position (U to A) (SD ─ , IC ↓ , SC )
10th Position MG1655
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 10th position (G to C)
No mutaion
10th position (G to C)
Mutation in the 10th position (U to A) (SD ─ , IC ↓ , SC )
10th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 10th position (G to C)
No mutaion
10th position (G to C)
Mutation in the 10th position (U to A) (SD ─ , IC ↓ , SC )
10th Position MG1655
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 10th position (G to C)
No mutaion
10th position (G to C)
Mutation in the 10th position (U to A) (SD ─ , IC ↓ , SC )
10th Position O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 11th position (A to U)
No mutaion
10th position (A to U)
Mutation in the 11th position (A to U) (SD ─ , IC ↓ , SC )
11th Position MG1655
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 11th position (A to U)
No mutaion
10th position (A to U)
Mutation in the 11th position (A to U) (SD ─ , IC ↓ , SC )
11th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 11th position (A to U)
No mutaion
10th position (A to U)
Mutation in the 11th position (A to U) (SD ─ , IC ↓ , SC )
11th Position MG1655
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 11th position (A to U)
No mutaion
10th position (A to U)
Mutation in the 11th position (A to U) (SD ─ , IC ↓ , SC )
11th Position O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 12th position (U to A)
No mutaion
10th position (U to A)
Mutation in the 12th position (U to A) (SD ─ , IC ↓ , SC ─ )
12th Position MG1655
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 12th position (U to A)
No mutaion
10th position (U to A)
Mutation in the 12th position (U to A) (SD ─ , IC ↓ , SC ─ )
12th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 12th position (U to A)
No mutaion
10th position (U to A)
Mutation in the 12th position (U to A) (SD ─ , IC ↓ , SC ─ )
12th Position MG1655
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 12th position (U to A)
No mutaion
10th position (U to A)
Mutation in the 12th position (U to A) (SD ─ , IC ↓ , SC ─ )
12th Position O157:H7
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 13th position (C to G)
No mutaion
10th position (C to G)
Mutation in the 13th position (C to G) (SD ─ , IC , SC )
13th Position MG1655
0 20 40 60 80 100 120 140 160 180 200-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 13th position (C to G)
No mutaion
10th position (C to G)
Mutation in the 13th position (C to G) (SD ─ , IC , SC )
13th Position O157:H7
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 13th position (C to G)
No mutaion
10th position (C to G)
Mutation in the 13th position (C to G) (SD ─ , IC , SC )
13th Position MG1655
390 392 394 396 398 400 402 404 406 408 410-9
-8
-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Mutataion in the 13th position (C to G)
No mutaion
10th position (C to G)
Mutation in the 13th position (C to G) (SD ─ , IC , SC )
13th Position O157:H7
0 50 100 150 200 250 300 350 400 450 500-12
-10
-8
-6
-4
-2
0
2
Minimum Free Energy when the algorithm is applied to a single sequence with using the original table
MG1655
Minimum Free Energy when the algorithm is applied to a single sequence with using a uniform table
0 50 100 150 200 250 300 350 400 450 500-12
-10
-8
-6
-4
-2
0
2
MG1655
0 50 100 150 200 250 300 350 400 450 500-7
-6
-5
-4
-3
-2
-1
Position
Ave
rage
Fre
e E
nerg
y (k
cal/m
ol)
Detected translation signals
Non coding (arbitrary)
Coding (translated)
Shine Dalgarno Signal
Initiation Signal
TerminationSignal
Detected Translation Signals when the algorithm is applied using a uniform table