simulations of visual deprivation in micebblais/pdf/pres_082704b.pdf · 2016. 7. 10. · on the...
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Simulations of Visual Deprivation in MiceSimulations of Visual Deprivation in Mice
Brian BlaisScott Kuindersma
Simulations of Visual Deprivation in MiceSimulations of Visual Deprivation in Mice
●Approximate Mouse Visual SystemApproximate Mouse Visual System●Normal RearingNormal Rearing●Model of DeprivationModel of Deprivation
● Monocular DeprivationMonocular Deprivation● Binocular DeprivationBinocular Deprivation
● On the order of 1000 photoreceptors feeding into 1 ganglion cell (Sterling 1988; Jeon, et al. 1998)
● Not much difference in cell density for mouse or cat retina● Retina/LGN responses show centersurround organization,
with a center diameter around 710o (Stone and Pinto 1993; Grubb and Thompson 2003) for the mouse [cat < 1o]
● mean V1 RF widths between 6o (Gordon et al. 1996) and 14o (Metin et al. 1998)
● Contralateral bias in mouse on the order of 2.5 (functionally)
Approximate Mouse Visual SystemApproximate Mouse Visual System
Natural ImagesNatural Images
6060oo
4040oo
● On the order of 1000 photoreceptors feeding into 1 ganglion cell32x32 photoreceptors input to ganglion cell
● A difference of Gaussians (DOG) retinal filter3:9 center:surround => center diameter ~ 13 pixels
● Resize image13 pixels ~ 7o (mouse)13 pixels ~ 0.5o (cat)
Pixels to PhotoreceptorsPixels to Photoreceptors
Mouse versus Cat Ganglion ResponsesMouse versus Cat Ganglion Responses
Unprocessed Mouse Cat
● Alter image intensityIpsilateral (right) channel image intensity is reduced by a factor of 2.5.
● Cats do not actually have a bias
Implementation of contralateral biasImplementation of contralateral bias
Mouse
Con
traIp
si
Cat (with bias)
Cat with contralateral bias – Normal Rearing (NR)Cat with contralateral bias – Normal Rearing (NR)
nr061504_1
Mouse NRMouse NR
nr081604_3_4
Mouse NRMouse NR
nr081704_3_1
Cat with bias – Monocular Deprivation (MD), High noise (lid suture)Cat with bias – Monocular Deprivation (MD), High noise (lid suture)
Gaussian SD = 0.8
md061604_1_8
Cat with bias – MD, Low noise (TTX)Cat with bias – MD, Low noise (TTX)
Gaussian SD = 0.01
md061604_1_01
MD, High noise (lid suture):● rapid deprivedeye response depression ● delayed nondeprived eye response potentiation
MD, Low noise (TTX): ● little or no deprivedeye response depression● rapid nondeprived eye response potentiation
MD noise dependencyMD noise dependency
Mouse – MD, High noise (lid suture)Mouse – MD, High noise (lid suture)
Gaussian SD = 0.8
md081704_2
Mouse – MD, Low noise (TTX)Mouse – MD, Low noise (TTX)
Gaussian SD = 0.01
md081704_1
● Pattern input into weaker eye● Noise input into stronger eye
Mouse MD Cat RS
(2.5/1 bias, DoG 3:9) (no bias, DoG 1:3)
Mouse MD (contralateral) is similar to Cat RSMouse MD (contralateral) is similar to Cat RS
Monocular Deprivation With Filtered ImagesMonocular Deprivation With Filtered Images
● A filtered image set + noise was applied to the deprived eye.
● These images have● Larger DoG filter● Decreased intensity
Goal: to generate a pattern of depression to delayed potentiation in the deprived channel.
Cat with bias – Filtered Image MDCat with bias – Filtered Image MD
md071504_2
Image SD 0.8, Noise SD 0.1
Cat with bias – Filtered Image MDCat with bias – Filtered Image MD
md071504_4
Image SD 0.8, Noise SD 0.2
Cat with bias – Filtered Image MDCat with bias – Filtered Image MD
md071504_1
Image SD 0.8, Noise SD 0.05
Mouse – Filtered Image MDMouse – Filtered Image MD
md081704_3_1
Image SD 0.8, Noise SD 0.05
Mouse – Filtered Image MDMouse – Filtered Image MD
md081704_4_1
Image SD 0.8, Noise SD 0.1
Effects of noise and retinal filter on MD dynamicsEffects of noise and retinal filter on MD dynamics
noisenoiselevellevel
finalfinaldeprivedeyedeprivedeyeresponseresponse
deprivedeyedeprivedeyeretinal filter sizeretinal filter size
deprivedeyedeprivedeyerecovery delayrecovery delay
Deprivation of the Ipsilateral EyeDeprivation of the Ipsilateral Eye
● Data?
Cat with bias – ipsilateral MD, high noiseCat with bias – ipsilateral MD, high noise
Gaussian SD = 0.8
md081904_4
Cat with bias – ipsilateral MD, low noiseCat with bias – ipsilateral MD, low noise
Gaussian SD = 0.01
md081904_3
Mouse – ipsilateral MD, high noiseMouse – ipsilateral MD, high noise
Gaussian SD = 0.8
md081904_6
Mouse – ipsilateral MD, low noiseMouse – ipsilateral MD, low noise
Gaussian SD = 0.01
md081904_5
Binocular DeprivationBinocular Deprivation
●BD for a week in an otherwise normal mouse BD for a week in an otherwise normal mouse has no effect on the response to either eye. has no effect on the response to either eye.
●I have an interesting twist for you.... [Misha] has I have an interesting twist for you.... [Misha] has found (so far) that subsequent BD completely found (so far) that subsequent BD completely erases the effect of the initial MD.erases the effect of the initial MD.
Mouse – Filtered Image BD following NRMouse – Filtered Image BD following NR
bd082504_4
Image SD 0.7, Noise SD 0.05
Mouse – Filtered Image MD Following MDMouse – Filtered Image MD Following MD
md082704_1
Image SD 0.8, Noise SD 0.05
MD MD
Mouse – Filtered Image BD Following MDMouse – Filtered Image BD Following MD
bd082704_1
Image SD 0.8, Noise SD 0.05
MD BD
SummarySummary
● Reproduce the results of Frenkel/Bear with BCM● Lid suture
● Rapid deprivedeye response depression● Delayed nondeprived eye response potentiation
● TTX● Little or no deprivedeye response depression● Rapid nondeprived eye response potentiation
● Filtered image deprivation● increasing noise level decreases final deprivedeye responses● increasing retinal filter size increases delay of recovery● BD gives no decrease after NR, and gives recovery after MD
● Ipsilateral deprivation● modest increase in the contralateral responses● standard BCMlike noise dependence for ipsi responses
Some Final ThoughtsSome Final Thoughts
● Blurry input patterns, caused by lidsuture, behave much like noise, when competing with nonblurry input patterns in another channel (e.g. MD)
● Small amounts of structure in blurry input patterns can somewhat offset the noiselike behavior, and can result in modest increases in responses
● When the only input is blurry input patterns (e.g. BD), then the situation is more like normal rearing, with (most likely) slower dynamics
Questions/Comments?Questions/Comments?