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CellVesselSingle-Usecustomised bioreactor

November 2014

Scalable platform for mammalian cell cultivation

World first configurable Single-Use-Bioreactor

• CellVesselcomesstandardinOD110,OD130,OD150,OD200,OD250forMagnetic-Stirrer-Table(MST)driveacceptingmostservomotors

• CellVesselisoptionallyavailableinOD110,OD130,OD150,OD200,OD250withHead-Plate-Drive(HPD)acceptingmostservomotors

• CellVesselwillsoonbeavailableinserioussize,suchasOD400

Configuration

Choose your own design =

fully configurable SUB

Scalable platform =

choose from 2 to >75 litre Vessel Volume

Outstanding performance =

>superior mixing capability

Manufactured entirely from

mono-component rigid plastics

Much more information available on www.cercell.com

Shownafewofthe4,000differentcomponentsfromtheConfigurator Tool abletosatisfyanyrequest

What do I get?

Choose what ever hose connections you want

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CERCELL.COM

CellVessel

Basic specifications for CellVessel are:• PolycarbonateandNyloncomponents(nomulti-componentplasticfilm!)

• NumberofstandardPG13.5portsaccordingtotheSUBdiameter

• 6differentdiametersand5differentheights

• Impeller(s)forbi-directionalrotation/up-flowcombinedaxial/radialfluidcirculation

• TheSUBbearingsdesignfor<500rpmfortwomonthcultivationprocess

Specification


Customised5.7litreSUBforHead-Plate-DrivenexttotheRe-Usable-Jacketforheatingpriortheinstallation.

Fully configurable CellVessel´s are created by selecting various optional components via the Configurator Tool• Arangeofimpeller(s),anyrotationorup-flow/down-flow/axial/radialfluidcirculationforanyapplication

•MSTdriveorHPDforconnectiontomostservomotors

• Temperaturecontrolledwithelectricalheatingblankets

• Temperaturecontrolledliquidjacket(RUJorSUJ)

• Variousaerationmethods;suchasmicroporespargers,holespargers,L-spargers,headspacegasexchange

• Tubestatorwithbafflesforaxialvortexmixing,donutshapeflowpatternforimprovedmasstransferandincreasedproductivity

• SeveraldifferentfluidIn&Outandexhaustmethods

• Arangeofsensors,Single-Use-Sensor(SUS),liquidlevel/foamsensors

• VariousconnectorslikefromColder,VPandothers

• DeliveredhighprecisionE-beam(32kGy)irradiatedindualpolyesterfoilbags

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CERCELL.COM

Customised identical 5.7 litre CellVessel

OD150 x 320 mm integrating,L-sparger,45degreetwinblade

Marineimpeller,Single-Use-Jacket.Individualcomponentsillustrated

onpage2.

Agitation in CellVesselImpellers


Impellersareaxialfluiddirectingrotat-ingelementsfordeliveringorabsorbingenergy.Theseelementswithingeneralfewandlargehelicoidalshapedbladesgiveveryhighdegreeofswirlinginavesselortube.Theflowpatterngener-atedinthefluidresembleshelixes.ImpellerdynamicscanbeCFDmodelledbybothBernoulli’sprincipleandNewton’sthirdlaw.

Impellersaredesignedwithindividualbladesattachedtothecentralcore.Describedviaprinciple,diameter,bladeangle,numberofblades.Principlesare:Pitch,Kidney,Marine,Helical

• Outside Diameter, OD mm: 40, 50, 60, 70, 80, 90, 100, 120 and 150

• Blade angle, degree to horizontal plane: 15, 30, 45

• Number of blades: 2, 3, 4

In practice more than 500 different impellers are available from the Configurator Tool

Unlimited range of impellers

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CERCELL.COM

Stators


Two identical 3.0 litre VV CellVessel viaConfigurator Toolequippedwithstator/baffels,100µmporesizemembraneequipedharvestlineandSUJ.DesignedforconnectiontoBiostatPCSviaHPDdriveandGL18connectionsfortemperaturecontrol.

Stators combined with baffles are obstructing vertical ar-ranged plates needed to stop the radial swirl inside the bioreactor and convert the rotational flow to efficient axial mixing.Withoutstators/baffles,thetangentialvelocitiescomingfromanyimpeller(s)causestheentirefluidmasstospincreatingacentralvortex.Thecombinationofthecircularstatortubeandouterbaffleplatess,sotospeak,increasethefrictiontothevesselinnerwallsurface.CellVesselbafflesarestraightflatplatesverticallyandperpendicularorientedexternalfromthecentralstatortubetotheinnersideofthevessel.

Tubestatorwithintegratedexternalbafflesconverttheotherwiselowefficiencyradialmixingtoaxialmixingforimprovedmasstransferandincreasedproductivity.

Tubestatorfurthersupports,gripsalltheverticallyarrangedtubesandsensorsbeneficialintallSUBs.TypicaloneofmanydifferencebetweentraditionalradialmixingandCellVesselCFDdesignedaxialmixingis30%bettermasstransfer.

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CERCELL.COM

A 3 litre CellVessel packed with five different sparger bodies allow the

end user to perform advanced areation

testing

Aeration in CellVessel

Ring sparger


Top class kLa values

Order a customized Test Kit

SelectedfromConfigurator Toolsection5classicalL-spargermountedhorizontalunderimpellerorstraightdownverticalorientedspargers.BothdesignequippedwithE-beam/Gammatolerantpolyeth-ylene(PE),Hydrophobicmembranes:Selectfrom:

•Micro-porousbodieswith15µmpores

•Macro-porousbodieswith100µmpores

•Macro-porousbodieswith250µmpores

• Supermacro-porousbodieswith500µmholes

Ringspargerwithmultipledrilled0.5mmholesintegratedinthebottomisoneofmanyoptions.

Gas in

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CERCELL.COM

Gas outSingle-Use Gas-Coolers combines inner boro silicate glass tube for excellent heat transfer and outer PC tube and cooling liquid connections. Thetransparentconstructionallowtheusertoinspectthefunctionality.

•Small size–ID/OD8/12x270mmglasstube,lowerendinletandoppositeoutletendfittedwithID/OD10/14mmsiliconehose,outletholdingoneormore26cm2surfaceareasterilefilter.Coolingareaof68cm2suitableformax120litre/hour/37°gasvolumecapacity.

•Medium size–ID/OD24/30x300mmglasstube,lowerendinletID/OD18/22mmsiliconehose,andoutletfittedwithID10/14mmsiliconehosepieceand400cm2surfaceareafilter.GlasstubefilledwithRaschig6x6mmglasstubeelements.Coolingarea500cm2suitableformax1200litre/hour/37°gasvolumecapacity.Coolingliquidin/outletbeingG1/8”forID6mmhoseconnection.

SelectedviatheConfigurator Tool


Animpeller/spargertestkitassembledfromarangeofcomponentscouldbeaguideforthedirectiontotakeinanewSUBdesign.

Order a

customized

Test Kit7

CERCELL.COM

Sensors and sampling

The photo show 10 customised CellVessel bio-reactors. In this case the In&Out system is equipped extensively with weldable hoses. ViatheConfigurator Tool youcanchooseanycombinationofhoses,connectorsforfluidspassingIn&OutoftheCellVesselheadplate.AsIn&OutrangeofaccessoriesisbasedonthePG13.5pluganywishispossibletofullfil.

Unlimited possibilities in connections

Configurable CellVessel bioreactor usethesameIn&OutsystemspecificationastheBactoVesselfermenter.

Hereshowntwocustomised3.0litreOD130x225forMSTdrive.ForsmallCellVesseltheMSTsolutionissligthlycheaper,veryfasttoinstallontheMSTandofferaverystableoperation.

Ingeneralthereisroomintheheadplatefor3PG13.5sizesensorsand8selectableliquid/gasportsfromtheIn&Outsystemwithhoseconnections.

Much more information available on

www.cercell.com

Sampling

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CERCELL.COM

Benefits:

• IntegratedSUSreducecontaminationrisk

• Savehoursofpreptimeandlabor,asnoautoclavingorsterilisationisneeded

• EnablesSUSsetuprightonthebenchtop–nobiosafetycabinet/hoodneeded

•Noautoclavingfacilityisrequired

• ChooseSUSviaConfigurator Tool

Single-Use-Sensors (SUS) offer many advantages

Bio mass sensors

• Capacitivesensingtech-nologyallowspreciseon-linemonitoringofthecellmass,viablecelldensityaswellascellphysiologicalstate.

• Userscanalsotrackcellcyclechanges,modelapoptosis,andpredictproteintiterallinrealtime

DO Single-Use-Sensor

• Non-invasiveDOwellforVisiFerm™.Pre-installedwithSUScapintegratedtotransparentPCtube.

• ExtendsDOsensorlife,assensorisneverautoclaved

•DOsensordesignedwithdigitalsignalcommunica-tionthatfitsmostPCS

pH Single-Use-Sensors

• Pre-installed6monthdrystoragetolerantpHSUSsuchasOneFermorFermProbe

• ClassicaldimensionpHsensorwithextendedshelflifetimeofferingmonthsofoperation

• ClassicalpHsignalfitsanyPCS

•Availablecombinedwith°CanalougeoutputfordualdigitalsignalcommunicationviaARCmodule

Single-Use-Sensors


ARC moule for SUS

• Pre-installedpHSUSOneFermVP6designedforconnectiontoARCanalogue/digitalmodule

• Availablecombinedwith°CanalougeoutputfordualdigitalsignalcommunicationviaARC

Express

installation

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CERCELL.COM

Weigth is an important factor


We recommed that you test the oppotunities with the configurable CellVessel SUB.

After you have found the best design and shown ability to bring along all your historical data. Then a frozen design can be offered in numbers and lifted to the cover design shown here.

This injection moulded cover presently available in size OD130 and OD150 offer 3 and 4 PG13.5 sensor ports, one gas in, dual gas exhaust, one set of level detection, and further 10 liquid/gas in/out ports.

Numbers is an important factor

Carrythe18kiloheavyautoclavedtraditionalglass/steel5litreSTRrequiresastrongman.Wherethelessthan2kiloSingle-Use-BioreactorCellVessel5.7litreSUBismuchmoreappropiateforawoman.

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CERCELL.COM

CellVessel heating options

CerCell offer from stock 150x350 mm silicone heating blanket which fits OD130, 150, 200 mm CellVessel and BactoVessel. Power rated at 110 watt from 230 VAC supply. The blanket has steel hugs at each ends and preferably a spring for tight attachment to the circumference of the SUB. If you give us information about the connector your PCS require we can even supply the heating element with connector.


Two identical 2 litre SUB on Magnetic-Stirrer-Tables equipped with 50x380 mm silicone heating blanket. Power rated at 110 watt from 230 VAC supply.

The use of two or more heating blankets allow end user to favor either visibility or vessel inner wall temperature according to needs.

Electrical

heating

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CERCELL.COM

OneofmanyCellVessel´snewconceptsaretomoveweightfromtheSTR,Stirred-Tank-Reactorsmadefromglass/StainlessSteeltostationarycomponents.ThetraditionaljacketedStirred-Tank-Reactorshaswith5.7litreVV(VesselVolume)typicallytheweightof18kilo.Notreallyeasytohandle!TheCellVesselSUFiskeptaslightaspossibleatlessthan2kiloasallweigthismovedintofixedcomponent–theRUJ!

Reduced

turn-around

time

Water connections

For water conveying Process-Control-System (PCS) CerCell offer a stand-alone Re-Usable-Jacket (RUJ) accessory to insert the SUB. For HPD drive only.

Specifications for RUJ:

•Operatingtemperature5-60°C

•Maxjacketoperatingpressure400mBar–RUJequippedwithaspringloadedover-pressure,systemprotectionvalvesetat500mBar

• Recommendedwaterflow1litre/minperlitre/WV(WorkingVolume)

• TwoG3/8”femalethreadforIn/Outadapters:

•Weightranging5-10kilodependingonsize,theweightfacilitatesstablearrangementondesktop.

• Thetransparentrigidplastictubematerialgivesexcellentprocessview.

Heating with Re-Usable-Jacket


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CERCELL.COM

CellVesselequippedwithSIngle-Use-JacketandfemaleG3/8”threadedconnectionsforavarietyofadapters.PhotoshownherewithG3/8”onSUBtoGL18adaptor(inwhite)forredBiostathoseconnection.

Fromlefttorigth:Rectus21toG3/8”coupledkit,G3/8”toOD10barbkit,G3/8”toGL18glassthreadkit,hoseadapterstoGL18kits

For water conveying Process-Control-System (PCS) theoptionalintegratedSingle-Use-Jacket(SUJ)heatexchangerisanefficientoptionfortheCellVesselfamilyofbioreactorsforMSTdrive.

Specifications for SUJ:

• SelectedviatheConfigurator Tool

• VerywellsuitedforMSTdrive

•Operatingtemperature5-60°Cheating/coolingwater

• Recommendedmaxoperatingpressureis500mBar

• Burstpressureat20°Cis<1Bar

• Recommendedcoolingflow1litre/min/WV

• StandardtwoG3/8”femalethreadconnect-ers

• Thetransparentrigidselfsupportiveplasticmaterialgivesexcellentprocessview.

Heating with Single-Use-Jacket

16.4 litre VV CellVessel (OD250x320) viaConfigurator Tool equippedwithSUJ.ThetwofemalethreadedG3/8”connectionsarevisibleontherightsideoftheSUF.



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CERCELL.COM

CellVessel 5.7 litrewithtwoMarineimpellersoperatingat500RPMmountedinRe-Usable-Jacket,equippedwithservomotorsupport,Sky-Supportforthegas-coolerandexhaustfilter,digitalsensorpackage.

Kollmorgen AKM23DservomotordrivingviaHPDinBiostatPCSsetupa5.7litreCellVesselsubmersedinaRUJ.HotstufftocomprehendthesometimesverywarmwatersuppliedfromwaterheatingPCS!


HPD drive• Head-Plate-Drive(HPD)forCellVesselSUBoffercoupling/adapterkitstoservomotorsfromApplikon®,Biostat®,DASGIP®,BJ,NBS,Solaris,Finesse®,BBI-biotechandotherPCSservomotors

• Re-usableservomotoradapterseasilyattachedtotheOD30mmHPDcentrebearingontheCellVesselheadplate

• ForhighloadsorsmallSUBaservomotorsupportishelpfulAgitation

methodsUse you

own PCS

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CERCELL.COM


MST drive•Magnetic-Stirrer-Table(MST)StainlessSteelconstructionfeaturinglonglifeandsilentrunningtoothedrubberbeltonaluminiumsprockets,twolifetimesealeddouble-rowballbearings

•MSTrotorisequippedwith12permanentmagnetsandabletotransfer>100wattpowerdependingontheparticularservomotorcapacity.Farsuf-ficientforanysizeCellVessel

• Dim:390x180x50mm,weightis5,7kilo

•MSTmostsuitableforallCellVesselwithelectricalheat-ingblanketorwithSingle-Use-Jacket(SUJ)forliquidheating

This particular MST is equipped with the B coupling for the variety ofservomotorstakingadvantageofID39-Badapter(latestmodelBiostat).ItsworthtomentionthatworkingwithMSTisveryfastandaconvenientsetup.Themosteconomicalchoiceforendurancework.

Kollmorgen AKM23D servomotordrivingMSTinBiostatsetupandthe11.6litreCellVesselwithstatorforhighefficiencyaxialmixing.

Mostservomotorsfacilitatethesecoupling.


You get drive options

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CERCELL.COM

SUB productsThe “Configurator Tool” can help you design a SUB to your User-Requirement-Specification

CellVessel sizes to select from

Vessel diameter, OD mm 110 130 150 200 250 400

Number of PG13.5 ports 7 7 9 10 10 10

Impeller diameter, OD mm 40-50 50-60 50-70 80-100 100-120 100-150

Vessel Volume, litre

225 mm 2.1 3.0 4.1

320 mm 4.2 5.7 11.6 16.4

420 mm 5.4 7.3 13.3 21.0

500 mm 8.6 15.7 24.8

600 mm 10.2 18.6 29.5 75.6

CerCellApsMalmmosevej19C

DK-2840HolteDenmark

www.cercell.comphone+4545423393

[email protected]

Thank you for your time

browsing this leaflet

You get our attention

Much more information available on

www.cercell.com

Specificationssubjecttochangewithoutnotice.Pleaserefertowww.cercell.com/intellectual-property-rights

regardingIPR,registrationsandtrademark.

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