SINKRONISATION SINKRONISATION

SPECIFIK INSTRUCTIONAL PURPOSESPECIFIK INSTRUCTIONAL PURPOSE

STUDENT CAPABLE TO STUDENT CAPABLE TO SYNCRONISATION ON LIVESTOCKSYNCRONISATION ON LIVESTOCK

INTRODUCTION• Tecnology Function in Farm :

Efficiency of Reproduction Conception Rate Calving Interval Puberty Age Service per Conception

Estrous Snap Make shorter of estrous cycle on a cow

Estrous Syncronisation Make shorter of estrous cycle on a group of livestock

ESTROUS SYNCRONISATIONESTROUS SYNCRONISATION

Make a group livestock estrous together Make a group livestock estrous together Artificial Insemination Artificial Insemination gestation gestation partus of calv with the same age partus of calv with the same age

Efficienci :Efficienci :

- cycle shorter- cycle shorter

- cost of insemination- cost of insemination

- easy maintenance- easy maintenance

For teraphy of disease For teraphy of disease

Analisys SWOT of technology Analisys SWOT of technology estrous sincronisationestrous sincronisation

1.1. STRONGSTRONG

2.2. WEAKNESSWEAKNESS

3.3. OPPORTUNITYOPPORTUNITY

4.4. THREATTHREAT

STRONGSTRONG- IS CHEAP TECHNOLOGYIS CHEAP TECHNOLOGY- NEED COMPETENT PERSONALNEED COMPETENT PERSONAL- NEW OPPORTUNITYNEW OPPORTUNITY- EFEKTIF FOR EFEKTIF FOR ↑↑ OF REPRODUCTION OF REPRODUCTION

EFFICIENCYEFFICIENCYWEAKNESSWEAKNESS

- PRICE OF HORMONE IS EXPENSIVEPRICE OF HORMONE IS EXPENSIVE- LOW OF GESTATIONLOW OF GESTATION- LOW INFORMATIONLOW INFORMATION- HIGHT COST OF OPERATIONAL FOR HIGHT COST OF OPERATIONAL FOR

LITTLE POPULATIONLITTLE POPULATION

OPPORTUNITYOPPORTUNITY

- PERFECTION FOR TECHNOLOGYPERFECTION FOR TECHNOLOGY

- NUMBER OF POPULATION NUMBER OF POPULATION ↑↑

- RERPODUCTION DISTURBANCE RERPODUCTION DISTURBANCE ↑↑

THREATTHREAT

- AI COST AI COST EXPENSIVE EXPENSIVE CHANGE CHANGE NATURAL MATTINGNATURAL MATTING

- TRADITIONAL BEHAVIOURTRADITIONAL BEHAVIOUR

HORMONE APLICATIONHORMONE APLICATION

PROGESTERONEPROGESTERONE

PROSTAGLANDHINE FPROSTAGLANDHINE F22

PRINCIPLEPRINCIPLE

ESTROUS SNAP WITH HORMONE ESTROUS SNAP WITH HORMONE PREPARAT BASED ON FEED BACK PREPARAT BASED ON FEED BACK PRINCIPLE FROM REPRODUCTION PRINCIPLE FROM REPRODUCTION

HORMONEHORMONE

PROGESTERONEPROGESTERONE

PRID (PROGESTIN RELEASE INTRAVAGINAL DEVICE)PRID (PROGESTIN RELEASE INTRAVAGINAL DEVICE)

SPIRAL HUMANSPIRAL HUMANCIDR (CONTROL INTRA VAGINAL DEVICE RELEASE)CIDR (CONTROL INTRA VAGINAL DEVICE RELEASE)PRIVASIS (P4 INTRA VAGINAL SILICONS SPONSPRIVASIS (P4 INTRA VAGINAL SILICONS SPONSIMPLANT CYLASTIC => SYNCROMED B IMPLANT CYLASTIC => SYNCROMED B KB SUSUKKB SUSUK

UNDER SKIN OF NECK / EARUNDER SKIN OF NECK / EARPRINCIPLE : AS CL SINTETICPRINCIPLE : AS CL SINTETIC

IMPLANT IN VAGINA 9-14 DAYSIMPLANT IN VAGINA 9-14 DAYSMIN DOSE 40 mg – 1 gMIN DOSE 40 mg – 1 g

AS DEPOPROVERA => CONTENT P4AS DEPOPROVERA => CONTENT P4THE SURLUS : DIDN’T SEE ESTROUS PHASETHE SURLUS : DIDN’T SEE ESTROUS PHASE

WEAKNESS :WEAKNESS :

DIDN’T COMFORTABLEDIDN’T COMFORTABLE IF CONTRACTION => IT’S GO OUTIF CONTRACTION => IT’S GO OUT BETTER : IMPLANTATION UNDER SKIN OF EARBETTER : IMPLANTATION UNDER SKIN OF EAR IMPLANT IN THE NECK IMPLANT IN THE NECK MAYBE DISAPPEAR MAYBE DISAPPEAR IM ???IM ??? HOW STOPPED?? HOW STOPPED?? SPECIALLY IN SOW SPECIALLY IN SOW BY FOOD DURING 9 DAYS BY FOOD DURING 9 DAYS GOOD ESTROUS GOOD ESTROUS P4 COMBINE WITH ESTRADIOL P4 COMBINE WITH ESTRADIOL

WHY ??? WHY ???

HOW ABOUT MECHANISME HOW ABOUT MECHANISME PROGESTERON APLICATIONPROGESTERON APLICATION PROSTAGLANDIN F2PROSTAGLANDIN F2 APLICATION APLICATION

PROSTAGLANDINE F2PROSTAGLANDINE F2APLICATION IN LUTEAL PHASEAPLICATION IN LUTEAL PHASETHE RULES : THERE IS CL & DIDN’T GRAVIDTHE RULES : THERE IS CL & DIDN’T GRAVIDPG = AS LEMAK, LOW BM = 400 Da So Can Used : I.Ut, PG = AS LEMAK, LOW BM = 400 Da So Can Used : I.Ut,

IM, I.Vul, I.Ovr, I.Vn (ABORTUS INDUKTION)IM, I.Vul, I.Ovr, I.Vn (ABORTUS INDUKTION)

COMMERCE PREPARAT :COMMERCE PREPARAT :

GLANDIN F, GLANDIN F,

LUTELYSE, LUTELYSE,

PROSOLVIN, PROSOLVIN,

PROSTAVETPROSTAVET

Lutheal Phase Foliculer Phase

16 – 17 days 4-5 days

PG WITH 1 X APLICATION17/21 x 100 ekor = 75%In Order 100% with 2 X APLICATION

PG 1 EST PG2 EST

2-3 Hr 9-14 Hr

75%100%

9 Days – 3 Days = 6 Days EARLY OF LUTEAL PHASE11 Days – 3 Days = 8 Days => in LUTEAL PHASE

11 DAYS

COWCOW GOAT/ EWEGOAT/ EWE

IMIM 20-25 mg20-25 mg 7,5 mg7,5 mg

I.UtI.Ut 7,5 mg7,5 mg 2,5 mg2,5 mg

I. VulI. Vul 7,5 mg7,5 mg 2,5 mg2,5 mg

I. OvrI. Ovr 0,2-0,5 mg0,2-0,5 mg --

DOSIS PROSTAGLANDIN F2DOSIS PROSTAGLANDIN F2

UTERUS

TUBAFALLOPII

KORPUS LUTEUM

OVARIUMVENA UTERINA

ARTERI OVARIKA MEMBELIT VENA

VENA ARTERITRANSFER

PG DARI ARTERIMERUSAK KL

SUPEROVULATIONSUPEROVULATION

↑ ↑ COUNT OF OVULATION IN 1 NORMAL COUNT OF OVULATION IN 1 NORMAL ESTROUS PERIODE ESTROUS PERIODE

NEED MANIPULATION TO NEED MANIPULATION TO ↑ ↑ OVUMOVUM

SUPEROVULASI INDUCTION USED SUPEROVULASI INDUCTION USED HORMONE PREPARAT HORMONE PREPARAT

COMBINE FROM GONADOTROPHINE COMBINE FROM GONADOTROPHINE HORMONEHORMONE

INDIVIDUAL RESPONSE INDIVIDUAL RESPONSE INSUPEROVULATION PROGRAME, INSUPEROVULATION PROGRAME,

DEPENDED ON : DEPENDED ON :

POTENTION &HORMONE DOSE POTENTION &HORMONE DOSE HORMON YANG DIBERIKANHORMON YANG DIBERIKAN

COMPARISON OF GONADOTROPHINECOMPARISON OF GONADOTROPHINE

FRECUENCY OF INJECTIONFRECUENCY OF INJECTION

SPECIES ANIMAL; FAMILY; AGE & SPECIES ANIMAL; FAMILY; AGE & FOODFOOD

HORMONE APLICATIONHORMONE APLICATION

COMBINATION OF HORMONECOMBINATION OF HORMONE

FSH & LHFSH & LH

PMSG & hCGPMSG & hCG

PMSG PMSG (PREGNANT MARE SERUM GONADOTROPHINE)(PREGNANT MARE SERUM GONADOTROPHINE)

* MARE GESTATION = 40 -120 DAYS.* MARE GESTATION = 40 -120 DAYS.

WHY?....FOR WHAT?....WHY?....FOR WHAT?....

* < 40 DAYS OR >120 DAYS DIDN’T HAVE PMSG. * < 40 DAYS OR >120 DAYS DIDN’T HAVE PMSG. WHY…?WHY…?

* IN GESTATION NEED P4 * IN GESTATION NEED P4 UNTIL 40 DAYS UNTIL 40 DAYS

ENAUGH FROM CL GRAVIDITATUMENAUGH FROM CL GRAVIDITATUM

IN 40 DAYSIN 40 DAYS NEED PMSG NEED PMSG FSH FSH

THE FOLICLE GROWTH BUT NOT THE FOLICLE GROWTH BUT NOT OVULATION OVULATION LUTEINISATION LUTEINISATION CL CL ASCESORIS ASCESORIS PRODUCE P4 PRODUCE P4

CL GRAVID + CL ASCESORIS CL GRAVID + CL ASCESORIS P4 P4 ↑↑

> 120 DAYS > 120 DAYS FUNCTION OF P4 FROM FUNCTION OF P4 FROM CL ASCESORIS CHANGED FROM CL ASCESORIS CHANGED FROM PLACENTA UNTIL PARTURITIONPLACENTA UNTIL PARTURITION

hCGhCG

(human Chorionic Gonadotrophine)(human Chorionic Gonadotrophine)

hCG hCG LH LH

from : HUMAN URINE IN GRAVID TRIMESTER I from : HUMAN URINE IN GRAVID TRIMESTER I

FUNCTION IN HUMAN : STIMULATION OF GONAD FUNCTION IN HUMAN : STIMULATION OF GONAD IN ORDER TO CL GRAVIDITATUM CONSIST TAKE IN ORDER TO CL GRAVIDITATUM CONSIST TAKE CARECARE

IN SUPEROVULATION : HELP LH ENDOGEN FOR IN SUPEROVULATION : HELP LH ENDOGEN FOR DISOSIASI FOLICLE WALL IN ORDER TO DISOSIASI FOLICLE WALL IN ORDER TO OVULATIONOVULATION

WHEN PMSG INJECTED??WHEN PMSG INJECTED??

IF FSH LOWER IN BLOOD (LUTEAL PHASE) IF FSH LOWER IN BLOOD (LUTEAL PHASE)

9 – 14 DAYS OF ESTROUS CYCLEUS BIRAHI9 – 14 DAYS OF ESTROUS CYCLEUS BIRAHI

IF FSH IF FSH ↑ ↑ DIDN’T BENEFIT DIDN’T BENEFIT

IN ORDER TO KNOW IN ORDER TO KNOW NEED ESTROUS NEED ESTROUS SYNCRONISATIONSYNCRONISATION

ONLY 1 INJECTEDONLY 1 INJECTED

• LONG ACTION = 108 HOURS (5-6 DAYS)LONG ACTION = 108 HOURS (5-6 DAYS)• ESTROGENIK EFECT ESTROGENIK EFECT DISTURB OF DISTURB OF IMPLANTATIONIMPLANTATION

• DOSE 2000 – 3000 IU (FOLIGON)DOSE 2000 – 3000 IU (FOLIGON)

• FOR OVUM PRODUCE FOR OVUM PRODUCE DIDN’T NEED DIDN’T NEED MATTINGMATTING• FOR ET FOR ET NEED MATTING NEED MATTING

• PHYSIOLOGIS CONDITION OF DONOR PHYSIOLOGIS CONDITION OF DONOR MUST THE SAME OF RESIPIENMUST THE SAME OF RESIPIEN

HOW ABOUT PMSG??HOW ABOUT PMSG??

DONOR

RESIPIEN

PG1 11Day PG2 9Day 2Day PG3 3Day EST 7Day Flushing

PMSG

PG1 11 Day PG2 3Day EST 7 Day Transfer

hCG IB Pagi

IB Sore 1/6 12/6 21/6 23/6 26/6 3/7

SUPEROVULATION with PMSG & hCG – Sinkron with PGSUPEROVULATION with PMSG & hCG – Sinkron with PG

DOSE of PMSG : 2000 – 3000 IUDOSE of hCG : 1500 -2000 IU

12/6 21/6 23/6 26/6 3/7

DONOR

RESIPIEN

PRID 9 Day 3 Day EST 7 Day Flushing

PMSG

PRID 9 Day 3 Day EST 7 Day Transfer

hCG IB Pagi

IB Sore

1/6 10/6 13/6 20/6

SUPEROVULATION (PMSG-hCG) - Sinkron (P4)SUPEROVULATION (PMSG-hCG) - Sinkron (P4)

DOSIS PMSG : 2000 – 3000 IUDOSIS Hcg : 1500 -2000 IUDOSIS P4 DALAM PRID = 40 mg – 1 g

1/6 10/6 13/6 20/6

OUT

KOMBINASI PG

BIRAHI LEBIH BAGUS

OUT

WHEN FSH INJECTED??WHEN FSH INJECTED??BOOSTER INJECTEDBOOSTER INJECTED

(4 DAYS: MORNING & EVENING) (4 DAYS: MORNING & EVENING)

EVERY 12 HOURSEVERY 12 HOURS

COUNT 40 mgCOUNT 40 mg

WITH DOSE DECREASE : 6, 5, 4 , 3 mgWITH DOSE DECREASE : 6, 5, 4 , 3 mg

IN ORDER TO KNOW IN ORDER TO KNOW NEED ESTROUS SYNCRONISATION NEED ESTROUS SYNCRONISATION

MASA KERJA PENDEKMASA KERJA PENDEK

S/ TDK BERSIFAT ESTROGENIKS/ TDK BERSIFAT ESTROGENIK

S/ PEROLEHAN EMBRIO DG KWALITAS LEBIH BAIKS/ PEROLEHAN EMBRIO DG KWALITAS LEBIH BAIK

U/ PROD. OVUM TDK PERLU DIKAWINKAN U/ PROD. OVUM TDK PERLU DIKAWINKAN

U/ ET PERLU DIKAWINKANU/ ET PERLU DIKAWINKAN

SYARAT KONDISI FISIOLOGIS DONOR=RESIPIENSYARAT KONDISI FISIOLOGIS DONOR=RESIPIEN

• SHORT ACTION = 12 HOURS SHORT ACTION = 12 HOURS DIDN’T ESTROGENIK EFECT DIDN’T ESTROGENIK EFECT

•KWALITAS EMBRYO IS BETTER GOODKWALITAS EMBRYO IS BETTER GOOD

•FOR OVUM PRODUCE FOR OVUM PRODUCE DIDN’T NEED DIDN’T NEED MATTINGMATTING

• FOR ET FOR ET NEED MATTING NEED MATTING

• PHYSIOLOGIS CONDITION OF DONOR PHYSIOLOGIS CONDITION OF DONOR MUST THE SAME OF RESIPIENMUST THE SAME OF RESIPIEN

HOW ABOUT FSH??HOW ABOUT FSH??

DONOR

RESIPIEN

PG1 11Day PG2 9 Day PG3 3Day EST 7Day Flushing

FSHPAGI

PG1 11 Day PG2 3Day EST 7Day Transfer

LH IB Pagi

IB Sore

1/6 12/6 21/6 22/6 23/6 24/6 27/6 4/7

SUPEROVULATION WITH FSH & LHSUPEROVULATION WITH FSH & LH

FSH I : 6 mgFSH II : 5 mgFSH III : 4 mgFSH IV : 3 MGTOTAL 40 mg

FSHPAGI

FSHPAGI

FSHPAGI

12/6 23/6 27/6 4/7

FSHSORE

FSHSORE

FSHSORE

FSHSORE