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Size Determination of DNA Restriction Fragments Laboratory BIOT 3250 Prof. Javier Cabello

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Page 1: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Size Determination

of DNA Restriction Fragments

Laboratory BIOT 3250

Prof. Javier Cabello

Page 2: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Experiment Objective

Page 3: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Materials

Page 4: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Materials

Page 5: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Requirements

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Concepts

Page 7: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Concepts (cont…)

Page 8: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Concepts (cont…)

Page 9: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Concepts (cont…)

Page 10: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Concepts (cont…)

Page 11: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Concepts (cont…)

Page 12: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Concepts

(cont…)

Page 13: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Concepts (cont…)

Page 14: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Flow Chart

Page 15: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis
Page 16: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis
Page 17: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

EXPERIMENT

Page 18: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis
Page 19: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis
Page 20: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis
Page 21: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Results

Page 22: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Conclusions

?

Page 23: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Questions

Page 24: Size Determination of DNA Restriction Fragmentsbiotecnologiamolecular.weebly.com/uploads/8/7/1/8/... · 2018. 10. 10. · DNA SAMPLES FOR ELECTROPHORESIS The DNA samples for electrophoresis

Reference