Care Specialties

SK-influx®

o Ceramides 1+3+6, cholesterol, fatty acids o Phytosphingosine

1Goldschmidt Personal Care

• A skin identical lipid concentrate

for an improved skin barrier function

• Ideal for dry, sensitive and aging skin

• A skin identical lipid concentrate

for an improved skin barrier function

• Ideal for dry, sensitive and aging skin

Care Specialties

The lipid barrier of the Stratum Corneum

Ceramides(40-65% total lipids)

Stratum Corneum:the "Bricks and Mortar" model

OHHN

O

2Goldschmidt Personal Care

Glucoceramides

OH

OHOH

O

HO

OHOH

OH

HN

OH

O

O

OH

cerabrosidase

Ceramides

40%

Cholesterol

30%

Cholesteryl

sulphate

5%Free fatty acids

25%

Care Specialties

The formation of the lipid barrier

StratumCorneum

Stratum

Multi-lamellar layer of ceramides and other skin lipids

Keratin Fibers

Stratum corneum

3Goldschmidt Personal Care

StratumBasal

StratumGranulosum

Lamellar bodies Stratum granulosum

Lamellar bodies

Care Specialties

Formation of the lipid barrier by the skin

Restoration of the lipid barrier by SK-influx®

SK-influx® mimics the natural formation

of the lipid barrier

Stratum

Applicaton

SK-influx®

4Goldschmidt Personal Care

Stratum

Corneum

Stratum

Granulosum

Care Specialties

Why is SK-influx® so efficient ?

Multi lamellar layers

Ceramides in Liquid

Crystalline form

SK-influx®

formulation

5Goldschmidt Personal Care

formulation

Lipid barrier in

the Stratum Corneum

Care Specialties

Characteristics of SK-influx®

1.5 % Ceramide complex

- Ceramide I 0.001%

- Ceramide III 0.5%

- Ceramide IIIB 0.5%

- Ceramide VI 0.5%

0.5 % Cholesterol

±3.5 % Free fatty acid

Composition SK-influx®

Lipid concentrate 6.0 %Water q.s.100 %Lactylate 10 %

Carbomer 0.3 %

Xanthan gum 0.3 %Methyl paraben 0.3 %

6Goldschmidt Personal Care

0.5% PhytosphingosineMethyl paraben 0.3 %Propyl paraben 0.2 %

Physical properties• Aqueous system

• Multi lamellar layers

• Liquid Crystalline form

Notes• Ceramides, cholesterol and fatty acids act synergistically

• According to scientific literature, Ceramides 1, 3 and 6 are specifically required to restore damaged and sensitive skin

INCI name:Ceramide 3; Ceramide 6 II; Ceramide 1; Phytosphingosine; Cholesterol; Sodium Lauroyl Lactylate; Carbomer; Xanthan Gum

Care Specialties

Designed for instant application

Typical dosages of SK-influx®

Normal skin 1.5 - 5 %Dry / Sensitive skin 3 - 5 %

7Goldschmidt Personal Care

Dry / Sensitive skin 3 - 5 %Aging skin 3 - 5 %

Skin repair / protection 3 - 15 %

• Suitable for a wide range of formulations

• SK-influx should be added to the water phase before the homogenisation step

• Cationic emulsifier systems should be avoided

Care Specialties

Uptake of Ceramide by the skinEx-vivo study

25

30

35

SK-influx® increases the bioavailability of Ceramide by more than 38 %, compared to O/W emulsion

SK-influx® increases the bioavailability of Ceramide by more than 38 %, compared to O/W emulsion

lab

ele

d C

era

mid

e V

I

8Goldschmidt Personal Care

0

5

10

15

20

s1+s2 s3+s4 s5+s6 s7+s8 s9+s10

Propyleneglycol/ ethanol

O/W polyglyceryl ester

O/W ethoxylated sorbitan ester

SK-influx like formulation

Tape strip number

Am

ou

nt

of

C14

lab

ele

d C

era

mid

e V

I

3x50 µµµµl of 4 different formulations with 0.5% of C14 labeled Ceramide VI are applied on isolated stratum corneum

Care Specialties

Repair of damaged barrier functionEx-vivo study

30

40

50

60

% r

ep

air

Addition of Cholesterol and Free fatty acids to Ceramides will have a synergistic effect

Addition of Cholesterol and Free fatty acids to Ceramides will have a synergistic effect

9Goldschmidt Personal Care

0

10

20

30

Vehicle

(based on

SK-influx)

Vehicle +Ceramide III

(based on

SK-influx)

SK-influx®

% r

ep

air

- Ex-vivo skin treated with acetone

- TEWL measured after 20 hours

will have a synergistic effect on the barrier repair of the skinwill have a synergistic effect on the barrier repair of the skin

Care Specialties

Why is Sk-influx essential for aging skin ?

Age

Hands

21 - 30 years31 - 40 years41 - 50 years

Ceramide level

100 %78 %63 %

10Goldschmidt Personal Care

41 - 50 years

Face

21 - 30 years31 - 40 years41 - 50 years

63 %

100 %62 %37 %

N.B. Relative levels of Ceramides 1 through 6 do not change.

Source: A.V. Rawlings et al. in Biocosmetics-Skin aging, IFSCC, vol. I, 31-45 (1993).

Care Specialties

Ceramides missing in the skin

Ceramide 1 , Ceramide 3 , Ceramide 6(Motta S et al., Arch. Dermatol. 130, 452 - 456 (1994))

Ceramide 1 , Ceramide 6

Why is Sk-influx essential for dry and sensitive skin conditions ?

Diseases

Psoriasis

Ichthyosis

11Goldschmidt Personal Care

Ceramide 1 , Ceramide 6(Paige DG et al., Proc. Br. Ass. Dermatol. 25 (1993))

Linoleate in Ceramide 1 (Ceramide 1 through 6 )

(Pershho K et al., J. Invest. Dermatol. 90, 350 - 353 (1988))

Ceramide 3 and 6(Di Nardo A et al., Acta Dermatol. Venereol. 78, 27 - 30 (1998))

Ceramide 3(Di Nardo A et al., Contact Dermatitis 35, 86 - 91 (1996))

Ichthyosis

Acne (vulgaris)

Atopic dermatitis

Surfactant-induced

dermatitis

Care SpecialtiesClinical evaluation- Multi center studyPart 1

0.5

1

1.5

2

2.5

Overall disease severity

Day 0

Week 4

Week 8

12Goldschmidt Personal Care

0

0.5

Allergic

Contact

Dermatitis

Irritant Contact

Dermatitis

Atopic

Dermatitis

Week 8

- Number of patients: Allergic Contact Dermatitis = 35, Irritant Contact Dermatitis = 123, Atopic Dermatitis = 24

- Product: W/O vehicle containing Ceramide 3, cholesterol and fatty acids in a nanoparticules technology.

- Application of the product: 1-2 times a day for maximum 8 weeks

- Evaluation of the symptoms: Randomly by dermatologists at day 0, week 4 and week 8

- Nature of the symptoms: Dryness, desquamation, erythema, pruritis, fissuring and overall severity

- Rating scale: 0=none; 1=mild; 2=moderate; 3=severe

- Number of patients: Allergic Contact Dermatitis = 35, Irritant Contact Dermatitis = 123, Atopic Dermatitis = 24

- Product: W/O vehicle containing Ceramide 3, cholesterol and fatty acids in a nanoparticules technology.

- Application of the product: 1-2 times a day for maximum 8 weeks

- Evaluation of the symptoms: Randomly by dermatologists at day 0, week 4 and week 8

- Nature of the symptoms: Dryness, desquamation, erythema, pruritis, fissuring and overall severity

- Rating scale: 0=none; 1=mild; 2=moderate; 3=severe

E. Berardesca et al., Contact Dermatitis, 2001, 45, 280-285. Evaluation of efficacy of a skin lipid mixture

in patients with irritant contact dermatitis, allergic contact dermatitis or atopic dermatitis: a multicenter study

Care Specialties

Clinical evaluation- Multi center studyPart 2

1

1.5

2

2.5

Allergic Contact Dermatitis

Day 0

Week 4

Week 8

1

1.5

2

Irritant Contact Dermatitis

Day 0

Week 4

Week 8

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0

0.5

1

Dryness Erytheme Fissuring

Week 8

0

0.5

Dryness Erytheme Fissuring

Week 8

- Number of patients: Allergic Contact Dermatitis = 35, Irritant Contact Dermatitis = 123, Atopic Dermatitis = 24

- Product: W/O vehicle containing Ceramide 3, cholesterol and fatty acids in a nanoparticules technology.

- Application of the product: 1-2 times a day for maximum 8 weeks

- Evaluation of the symptoms: Randomly by dermatologists at day 0, week 4 and week 8

- Nature of the symptoms: Dryness, desquamation, erythema, pruritis, fissuring and overall severity

- Rating scale: 0=none; 1=mild; 2=moderate; 3=severe

- Number of patients: Allergic Contact Dermatitis = 35, Irritant Contact Dermatitis = 123, Atopic Dermatitis = 24

- Product: W/O vehicle containing Ceramide 3, cholesterol and fatty acids in a nanoparticules technology.

- Application of the product: 1-2 times a day for maximum 8 weeks

- Evaluation of the symptoms: Randomly by dermatologists at day 0, week 4 and week 8

- Nature of the symptoms: Dryness, desquamation, erythema, pruritis, fissuring and overall severity

- Rating scale: 0=none; 1=mild; 2=moderate; 3=severe

E. Berardesca et al., Contact Dermatitis, 2001, 45, 280-285. Evaluation of efficacy of a skin lipid mixture

in patients with irritant contact dermatitis, allergic contact dermatitis or atopic dermatitis: a multicenter study

Desquamation Pruritis Desquamation Pruritis

Care Specialties

Clinical evaluation- Multi center studyPart 3

1

1.5

2

Atopic Dermatitis

Day 0

Week 4

Week 8

“Balanced lipid mixtures [Ceramides, cholesterol, fatty

acids] are effective in improving the barrier properties and the

clinical condition of the skin in

“Balanced lipid mixtures [Ceramides, cholesterol, fatty

acids] are effective in improving the barrier properties and the

clinical condition of the skin in

14Goldschmidt Personal Care

0

0.5

Dryness Erytheme Fissuring

Week 8

- Number of patients: Allergic Contact Dermatitis = 35, Irritant Contact Dermatitis = 123, Atopic Dermatitis = 24

- Product: W/O vehicle containing Ceramide 3, cholesterol and fatty acids in a nanoparticules technology.

- Application of the product: 1-2 times a day for maximum 8 weeks

- Evaluation of the symptoms: Randomly by dermatologists at day 0, week 4 and week 8

- Nature of the symptoms: Dryness, desquamation, erythema, pruritis, fissuring and overall severity

- Rating scale: 0=none; 1=mild; 2=moderate; 3=severe

- Number of patients: Allergic Contact Dermatitis = 35, Irritant Contact Dermatitis = 123, Atopic Dermatitis = 24

- Product: W/O vehicle containing Ceramide 3, cholesterol and fatty acids in a nanoparticules technology.

- Application of the product: 1-2 times a day for maximum 8 weeks

- Evaluation of the symptoms: Randomly by dermatologists at day 0, week 4 and week 8

- Nature of the symptoms: Dryness, desquamation, erythema, pruritis, fissuring and overall severity

- Rating scale: 0=none; 1=mild; 2=moderate; 3=severe

clinical condition of the skin in contact dermatitis.”

clinical condition of the skin in contact dermatitis.”

E. Berardesca et al., Contact Dermatitis, 2001, 45, 280-285. Evaluation of efficacy of a skin lipid mixture

in patients with irritant contact dermatitis, allergic contact dermatitis or atopic dermatitis: a multicenter study

PruritisDesquamation

Care Specialties

Advantages SK-influx®

• The best available lipid mix:

– Ceramides 1+3+6, cholesterol, fatty acids

– Phytosphingosine (anti-inflammation and anti-microbial properties)

15Goldschmidt Personal Care

• Resembles the structure of the lipid barrier in the skin

– multi-lamellar

– liquid crystalline

• Designed to allow uptake of Ceramides by the skin

• Ideal for dry, sensitive and aging skin