Solutions for

Cancer Metabolism Research

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Assay Kits, Metabolites, and Reagents Specific to Your Pathway of Interest1. Aerobic Glycolysis

2. Fatty Acid Metabolism

3. Mevalonate Pathway

4. Glutamine Metabolism

and More

sigma.com/cancermetabolism

2 Solutions for Cancer Metabolism Research

Advantages:• Rapid and high throughput ready

• Reproducible and supported by industry-leading technical service

• Contain all reagents, probes, and standards required for assay

• Colorimetric and fluorometric formats

• Economical pricing

• Offered with associated metabolites and reagents

METABOLISM ASSAY KITS FROM SIGMA-ALDRICH®

Convenient results you can trust.

For further info visit sigma-aldrich.com/cancermetabolism

3For more information, visit sigma-aldrich.com

Cat. No. NameL2625 l-Lactic Dehydrogenase from bovine heart Type III

L1378 l-Lactic Dehydrogenase from bovine muscle Type X

Cat. No. NameG9545 Anti-GAPDH antibody produced in rabbit, ~1 mg/ml, affinity

isolated antibody, buffered aqueous solution

SAB1100050 Anti-LDHA (216-228) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

SAB4200095 Anti-PKM2 (isoform M2) antibody produced in rabbit, ~1.5 mg/ml, affinity isolated antibody

Cat. No. NameGAGO20 Glucose (GO) Assay Kit, sufficient for 20 tests

GAHK20 Glucose (HK) Assay Kit, sufficient for 20 tests

MAK064 Lactate Assay Kit, sufficient for 100 colorimetric or fluorometric tests

MAK066 Lactate Dehydrogenase Activity Assay Kit, sufficient for 500 colorimetric tests

MAK065 Lactate Assay Kit II, sufficient for 100 colorimetric tests

MAK037 NAD/NADH Quantitation Kit, sufficient for 100 colorimetric tests

MAK039 Acetyl-Coenzyme A Assay Kit, sufficient for 100 fluorometric tests

MAK083 Glucose Uptake Colorimetric Assay Kit, sufficient for 100 colorimetric tests

MAK091 Hexokinase Colorimetric Assay Kit, sufficient for 100 colorimetric tests

MAK071 Pyruvate Assay Kit, sufficient for 100 colorimetric or fluorometric tests

1. AEROBIC GLYCOLYSIS AND THE WARBURG EFFECT

Investigation of the metabolic basis of oncogenesis continues with fervor in the field of cancer research. Although the Warburg effect was discovered in 1924, aerobic glycolysis remains a central pathway of interest in cancer metabolism studies. 1,2

1. Boroughs, L. K. et al. Metabolic Pathways Promoting Cancer Cell Survival and Growth. Nature Cell Bio. 17, 351-359 (2015).

2. Seyfried, T. N. et al. Cancer as a Metaoblic Disease: Implications for Novel Therapeutics. Carcinogenesis, 35, 515-527 (2014)

Metabolites, Inhibitors, and Activators of Aerobic Glycolysis

Proteins Involved in Aerobic Glycolysis

Antibodies Against Proteins Involved in Aerobic Glycolysis

Cat. No. Name DescriptionG7528 d-(+)-Glucose BioXtra, ≥99.5% (GC) Metabolite

L7022 Sodium l-lactate ~98% Metabolite

P5280 Sodium pyruvate powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Metabolite

G7879 d-Glucose 6-phosphate sodium salt Sigma Grade, crystalline

Metabolite

A9978 AICAR ≥98% (HPLC), powder Inhibitors and Activators of the AMPK pathway

D150959 1,1-Dimethylbiguanide hydrochloride 97%

Inhibitors and Activators of the AMPK pathway

P5499 Dorsomorphin ≥98% (HPLC) Inhibitors and Activators of the AMPK pathway

D9533 Deferoxamine mesylate salt powder, ≥92.5% (TLC)

HIF Prolyl-Hydroxylase Inhibitor

D3695 DMOG ≥98% (HPLC) HIF Prolyl-Hydroxylase Inhibitor

D8375 2-Deoxy-d-glucose ≥98% (GC), crystalline

Hexokinase Inhibitor

View our complete cancer metabolism research portfolio at sigma.com/cancermetabolism

Assay Kits for Studying Aerobic Glycolysis Metabolites

4 Solutions for Cancer Metabolism Research

2. FATTY ACID METABOLISM IN CANCEROncogenic progression is often associated with the increased activity and expression of enzymes involved in fatty acid synthesis, such as fatty acid synthase (FASN), acetyl-CoA carboxylase (ACC), and ATP citrate lyase (ACL).

Assay Kits for Studying Fatty Acid Metabolism

Inhibitors of Fatty Acid Biosynthesis Pathways

Metabolites of Fatty Acid Biosynthesis Pathways

Cat. No. NameMAK044 Free Fatty Acid Quantitation Kit, sufficient for 100 colorimetric or

fluorometric tests

MAK039 Acetyl-Coenzyme A Assay Kit, sufficient for 100 fluorometric tests

MAK085 Lipid Peroxidation (MDA) Assay Kit, sufficient for 100 colorimetric or fluorometric tests

MAK086 Acetate Colorimetric Assay Kit, sufficient for 100 colorimetric tests

MAK041 β-Hydroxybutyrate Assay Kit, sufficient for 100 colorimetric tests

Cat. No. NameE4143 (−)-Epigallocatechin gallate ≥95%

Q4951 Quercetin ≥95% (HPLC), solid

C2389 Cerulenin ≥98% (HPLC), from Cephalosporium caerulens

A3145 Apigenin ≥97% (TLC), from parsley, powder

Cat. No. NameA2056 Acetyl coenzyme A sodium salt ≥93% (HPLC), powder

N7505 β-Nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt hydrate ≥97% (HPLC)

O4126 Oxaloacetic acid ≥97%

P9767 Sodium palmitate ≥98.5%

F1506 Sodium fumarate dibasic ≥99%

M4263 Malonyl coenzyme A lithium salt ≥90% (HPLC)

View our complete cancer metabolism research portfolio at sigma.com/cancermetabolism

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Proteins Involved in the Mevalonate PathwayCat. No. NameH7039 3-Hydroxy-3-methylglutaryl-CoA reductase human solution

M6908 Myeloperoxidase from human leukocytes

View our complete cancer metabolism research portfolio at sigma.com/cancermetabolism

Assay Kits for Studying the Mevalonate Pathway

Metabolites, Inhibitors, and Activators of the Mevalonate Pathway

Cat. No. NameMAK043 Cholesterol Quantitation Kit, sufficient for 100 colorimetric or

fluorometric tests

CS1090 HMG-CoA Reductase Assay Kit, sufficient for 30 assays

MAK069 MPO Fluorometric Activity Assay Kit, sufficient for 100 fluorometric tests

Cat. No. Name DescriptionG6025 Geranylgeranyl pyrophosphate

ammonium salt solution, ≥95% (TLC), ~1 mg/mL in methanol: NH4OH (7:3)

Metabolite

F6892 Farnesyl pyrophosphate ammonium salt methanol:ammonia solution, ≥95% (TLC)

Metabolite

S6196 Simvastatin ≥97% (HPLC), solid Inhibitor of HMG-CoA Reductase

M2537 Mevastatin ≥95% (HPLC), powder Inhibitor of HMG-CoA Reductase

SML0223 Zoledronic acid monohydrate ≥98% (HPLC)

Inhibitor of Farnesyl diphosphate synthase

3. MEVALONATE PATHWAY IN CANCERThe mevalonate pathway, associated with a diversity of cellular functions, also continues to be vigorously studied for its role in various types of cancer. 1,2

1. Jiang, P. In vitro and In Vivo Anticancer Effects of Mevalonate Pathway Modulation on Human Cancer Cells. Br. J. Cancer, 111, 1562-1571 (2014).

2. Yeganeh, B. Targeting the Mevalonate Cascade as a New Therapeutic Approach in Heart Disease, Cancer and Pulmonary Disease. Pharmacol. Ther. 143, 87-110 (2014).

NAVIGATE TOWARD METABOLOMIC DISCOVERY.Target your metabolism research with Sigma® Life Science’s comprehensive metabolomic resources.

Bionavigate.Sigma Life Science metabolites, enzymes, separation tools and technologies help you navigate the metabolic pathways to biomarker discovery. Our comprehensive offering of unique isolated and synthetic metabolites includes the amino acid, carbohydrate, lipid and TCA Cycle metabolite libraries.

Now featuring tandem mass spectrometry data from The Scripps Center for Metabolomics METLIN Metabolite Database for several metabolites.

Find metabolites for your research needs sigma.com/metabolomics

6 Solutions for Cancer Metabolism Research

Assay Kits for Studying Glutamine Metabolism

Metabolites, Inhibitors, and Activators of Glutamine Metabolism

Cat. No. NameMAK037 NAD/NADH Quantitation Kit, sufficient for 100 colorimetric tests

AA0100 Ammonia Assay Kit

MAK039 Acetyl-Coenzyme A Assay Kit, sufficient for 100 fluorometric tests

MAK004 Glutamate Assay Kit, sufficient for 100 colorimetric or fluorometric assays

GLN1 Glutamine and Glutamate Determination Kit

Cat. No. Name DescriptionG7513 L-Glutamine solution 200 mM,

solution, sterile-filtered, BioXtra, suitable for cell culture

Metabolite

A2056 Acetyl coenzyme A sodium salt ≥93% (HPLC), powder

Metabolite

G8415 L-Glutamic acid from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-100.5%

Metabolite

D2141 6-Diazo-5-oxo-L-norleucine crystalline

Inhibitor of various glutaminases

C13408 O-(Carboxymethyl)hydroxylamine hemihydrochloride 98%

Inhibitor of Aminobutyrate aminotransferase

View our complete cancer metabolism research portfolio at sigma.com/cancermetabolism

4. GLUTAMINE METABOLISM IN CANCERMany of the signaling pathways that promote oncogenesis also reprogram glutamine metabolism. In particular, cancer cells commonly rely on glutamate anaplerosis to refill the TCA cycle.

New Interactive Metabolomic Pathway ToolNow your metabolites, enzymes, and selected pathways are searchable and interactive!

sigma.com/pathways

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and cutting-edge gene editing technology

• Create diverse and unique compounds with the largest portfolio of building blocks, catalysts and reagents

• Accelerate novel compound discovery with chemically diverse compound arrays and screening libraries

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