• Species:• Leptodea fragilis (wetlands/river mouths)• Pyganodon grandis (wetlands/nearshore/tribs – Lake Erie)• Quadrula quadrula (wetlands/river mouths – Lake Erie)• Lampsilis siliquoidea/radiata (Lake St. Clair drainage only)• Ligumia nasuta (where it can be found 1-2 swab samples/refuge)

• Numbers (site = refuge scale):• Microsats – clips only:

• Q. quadrula (at least 10, but <50/site)• L. fragilis (at least 10, but <50/site)• L. siliquoidea (at least 10, but <50/site)

• mtDNA (m/f lineage) – sacrifice animals• P. grandis (15 males/site)• L. fragilis (15 males/site, subsampled from above) – juveniles?

• Swabs only (endangered species)• L. nasuta (1-2 swabs/site)

Genetic Sampling

• Clipping instructions (more at methods meeting):• pry open shell with blunt end of scalpel/spatula (no blade!)• use thumb or end of pencil or small rubber stopper to

wedge open shell• clip ~0.5 cm2 piece of mantle tissue from shell edge using

surgical scissors (from basic dissection kit – keep them sharp)• clip from ventral-anterior side (avoid siphons)

• Swab samples• as above, but sterile swab 2-3 times along foot – avoid touch

mantle and siphons

Logistics

• Zanatta lab:– 2 grad students + 2 undergrads– 2 teams will join field teams as dedicated

clippers/swabbers• Krebs lab:– Trevor Prescott + undergrad will assist in clipping

• 3 genetics teams to assist other with general field work and will do clipping in field