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SSME-518SSME-518Instructional Technology and Material Instructional Technology and Material

DevelopmentDevelopment

Assignment2

Presented by: Harika Özge KızılsuPresented by: Harika Özge Kızılsu

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OutlineOutline

Introduction to enzymes

How enzyme work Activation energy

Factors effecting enzyme activity Temperature pH Enzyme concentration Substrate concentration

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Introduction to enzymesIntroduction to enzymes

• Enzymes are usually proteins

• Enzymes are catalysts

• In general, names end with suffix “ase”

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Introduction to enzymesIntroduction to enzymes

CatalystCatalyst Time for reactionTime for reaction

Yes 1 second

No 3 x 10 12 years

3 x 1012 years is 500 times the age of the earth!!!!

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Introduction to enzymesIntroduction to enzymes

Enzymes:

• increase the rate of a reaction

• not consumed by the reaction

• act repeatedly to increase the rate of reactions

• are often very “specific” for the substrate

Reactants also called “substrates” of enzyme

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How enzyme workHow enzyme work

• Enzymes physically interact with their substrates

• Substrates bind to the enzyme’s active site

• Enzyme reaction path:

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How enzyme workHow enzyme work

• Lock and Key Model

Substrate fits into an active site perfectly. (key)(key) (lock) (lock)

click to see animation of model

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• Activation energy is:

the energy required to start a reaction.

How enzyme workHow enzyme work

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Factors effecting enzyme activityFactors effecting enzyme activity

Temperature

pH

Enzyme concentration

Substrate concentration

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TemperatureTemperature

36

0 10 20 30 40 50 60 70

Temperature

Rat

e of

rea

ctio

n

C (optimum temperature)

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pHpH

0 1 2 3 4 5 6 7 8 9

pH

Rate

of r

eact

ion

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Enzyme concentrationEnzyme concentration

Enzyme concentration

Rate

of r

eact

ion

Substrate concentration constant

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Substrate concentrationSubstrate concentration

click to learn more

Substrate concentration

Rat

e o

f re

acti

on

Enzyme concentration constant

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Thank YOU Thank YOU

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ReferencesReferences

• http://fig.cox.miami.edu/~cmallery/255/255enz/enzymology.htm

• http://ntri.tamuk.edu/cell/enzyme2.html

• http://www.animationfactory.com/animations/buildings/doors/6038e/