stability test sheet

8/6/2019 Stability Test Sheet

1/14

14-Jul 16-Jul 18-Jul 20-Jul 22-Jul 25-Jul 28-Jul

DAY 0 2 4 6 8 11 14

PARAOXON 23.5 PTE 196.46 193.67 189.98 194.42 203.71 194.73 194.39198.15 191.91 192.91 198.29 203.00 194.91 192.80

Average 197.31 192.79 191.45 196.36 203.36 194.82 193.60

23.5 Control 229.59 230.00 229.20 225.45 228.77 227.95 228.41

4 PTE 198.04 175.09 186.80 205.38 198.19 202.14 190.69

198.96 192.66 194.76 195.18 204.59 197.88 200.13

Average 198.50 183.88 190.78 200.28 201.39 200.01 195.41

4 Control 230.22 230.00 230.19 230.36 229.69 227.82 229.03

-15.5 PTE 191.25 190.45 173.62 202.96 200.00 201.86 199.79

202.84 187.42 191.05 197.84 198.98 200.98 197.07

Average 197.05 188.94 182.34 200.40 199.49 201.42 198.43

-15.5 Control 228.48 231.03 232.38 226.98 229.84 229.47 230.69

PARATHION 23.5 PTE 204.50 190.67 193.72 183.20 202.10 196.80 204.51

197.23 195.85 193.84 202.23 208.75 198.57 201.43 Average 200.87 193.26 193.78 192.72 205.43 197.69 202.97

23.5 Control 233.95 231.30 229.17 225.33 227.47 227.65 227.02

4 PTE 197.78 196.38 195.10 205.60 202.69 204.44 205.70

202.40 194.57 192.47 197.51 196.67 199.43 201.30

Average 200.09 195.48 193.79 201.56 199.68 201.94 203.50

4 Control 229.64 231.81 229.55 231.86 231.23 230.50 230.60

-15.5 PTE 204.30 194.18 181.98 203.55 197.90 209.55 186.71

197.63 185.69 191.01 199.48 186.68 206.12 204.59

Average 200.97 189.94 186.50 201.52 192.29 207.84 195.65


2/14

Notes:

- Day 0 colour tested after 15 min incubation at approx. 5:30pm, thereafter colour allowed to develop over 15 mi- Paraoxon and parathion blue light value in ImageJ used to represent change in yellow colour

- Coumaphos red, green, blue light colour values recorded to detect blue fluoresence change

- For coumaphos the photos taken typically show up a bright white blue fluorescence and in Image J the green

DAY 0 2 4 6 8 11 14

Coumaphos 23.5 PTE 1 1 1 1 1 1 1

(qualitative) 23.5 control 0 0 0 0 0 0 0

4 PTE 1 1 1 1 1 1 1

4 control 0 0 0 0 0 0 0

-15.5 PTE 1 1 1 1 1 1 1

-15.5 control 0 0 0 0 0 0 0

- Using PTE purified from Pseudomonas putida, with pVLT-31 + opdgene


3/14

31-Jul 4-Aug 9-Aug 13-Aug 18-Aug 23-Aug

17 21 26 30 35 40

196.10 197.93 193.85 196.72 196.41 188.04192.66 188.64 199.75 196.09 193.03 188.35

194.38 193.29 196.80 196.41 194.72 188.20

224.54 222.79 226.70 225.05 224.32 226.21

190.32 203.75 188.61 180.75 198.13 184.84

189.32 202.54 191.44 199.48 188.57 187.27

189.82 203.15 190.03 190.12 193.35 186.06

226.92 220.26 224.17 224.68 221.89 218.04

196.33 187.53 192.14 195.60 199.52 174.81

191.97 202.43 196.90 196.72 198.69 187.26

194.15 194.98 194.52 196.16 199.11 181.04

229.15 223.69 226.84 224.75 228.58 221.77

208.39 199.36 199.67 190.40 180.85 191.69

202.02 197.43 197.01 197.73 195.90 -205.21 198.40 198.34 194.07 188.38 191.69

228.31 224.65 226.00 225.48 222.73 231.37

205.31 204.14 200.40 204.23 201.69 180.73

189.30 201.16 201.92 205.20 203.97 191.54

197.31 202.65 201.16 204.72 202.83 186.14

227.72 224.72 220.64 229.29 226.21 225.23

193.31 201.16 189.20 204.19 193.73 173.90

203.22 197.06 196.14 204.75 202.51 -

198.27 199.11 192.67 204.47 198.12 173.90


4/14

in. Day 2 and 4 were incubated longer, approx 30 min

nd blue values max out at 255.

Preformed by Chris Langley and

17 21 26 30 35 40

1 1 1 1 1 1

0 0 0 0 0 0

1 1 1 1 1 1

0 0 0 0 0 0

1 1 1 1 1 1

0 0 0 0 0 0


5/14


6/14

avid Collings beginning July 14th, 2010


7/14


8/14


9/14


10/14


11/14

0 10 2 0 30 4 0

170.00

180.00

190.00

200.00

210.00

220.00

230.00

240.00

Immobilized PTE Stability Test - 1mM Paraoxon

23.5 PTE 4 PTE -15.5 PTE 23.5 Control 4 Control -15.5 Control

Days

Meanblueh

istog

ram

value


12/14

0 10 2 0 30 4 0

170.00

180.00

190.00

200.00

210.00

220.00

230.00

240.00

Immobilized PTE Stability Test - 1mM Parathion

23.5 PTE 4 PTE -15.5 PTE 23.5 Control 4 Control -15.5 Control

Days

Meanbluehistog

ram

va

lue


13/14

0 5 10 15 20 25 30 35

0.00

50.00

100.00

150.00

200.00

250.00

300.00

1mM COUMAPHOS Blue

-15.5 Control Row 36 Row 41 Row 46 Row 42 Row 37

Days

Meanbluedetection


14/14

0 5 10 15 20 25 30 35 40

-0.2

0

0.2

0.4

0.6

0.8

1

1.2

Immobilized PTE Stability Test - 1mM Coumaphos (Qualitative)

23.5 PTE

4 PTE

-15.5 PTE

23.5 control

4 control

-15.5 control

Days

Visiblefluorescenceun

derUVhandheld

stability test sheet

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