stem celltherapy
TRANSCRIPT
The Hematopoietic Stem Cell Therapy for Exploration of Space
Seigo Ohi, PhDAssoc Prof and Molec Geneticist, Depts Biochem & Molec Biol, Genetics &
Hum Genet, Pediatrics & CH, and Center for Sickle Cell Disease, Col of Medicine and Grad School
Howard University and Hospital, Washington, DCSupported by: NASA Institute for Advanced Concepts/USRA
Interplanetary Space Exploration:A) Readiness for catastrophic disorders:
- Hematological and cardiac abnormalities- Immunodeficiency- Muscle loss- bone losses- Neurological disorders - Cancer
B) Countermeasures:- Hematopoietic stem cell therapy (HSCT)- Gene therapy- Others, e.g. drug therapy
C) Robotization: Robotic medicine, e.g. Robotic HSC injection machine
OUR AIMS ARE TO DEVELOP THE HEMATOPOIETIC STEM CELL THERAPY AND GENE
THERAPY FOR ASTRONAUTS TO COUNTERMEASURE
SEVERE/INVASIVE DISORDERS CAUSED BY SPACE ENVIRONMENTS.
Multiple Differentiating Potential of Adult Stem Cells
Adapted from: Vogel G, Science 290:1674 (2000)
Possible Benefits of HSCT• Hematological disorders:
– Space anemia– Immunodeficiency
• Muscle and bone losses• Neurological disorders• Cancer Therapy:
– Bone marrow replacement therapy for blood-born cancer, e.g. leukemia
– High dose chemotherapy
Multiple Differentiating Potential of Adult Stem Cells
Adapted from: Vogel G, Science 290:1674 (2000)
THE PROTOCOL
• Genetically mark HSCs with β-galactosidasegene: Transfection or use of Tg-mice.
• For β-gal Tg-mice: Isolate and expand HSCs; Strain 129S.
• Transplant the HSCs to 129S hindlimb-suspended mouse: Injection into the right leg.
• Isolate tissues from the recipient and stain by X-gal.
• Examine leg tissues for the blue stain.• Fine structural analysis of muscles.
X-gal Staining of Normal- and β-gal-mouse Tissues
A. B. C. D. E. F.Intstn. Kdny. Liver Spln. Heart Lung
PREVENTIVE MEDICINE based on human / robotic synergistic diagnosis and
minor operations:
Hematopoietic Stem Cell Therapy and Gene Therapy
ACKNOWLEDGEMENTS- Howard U.: J. Brezo, A.N. Roach, B.C. Kim
- NASA-NIH: W. Fitzgerald, L. Margolis, J. Zimmerberg
- Medical Col. of Wisconsin: D. Riley
Supported by: NASA Institute for Advanced Concepts/USRA